Strain identifier

BacDive ID: 16815

Type strain: No

Species: Moorella thermoacetica

Strain Designation: ET-5a

Strain history: <- H. L. Drake, University of Bayreuth, Department of Ecological Microbiology, Bayreuth, Germany; ET-5a <- H. L. Drake and A. Goessner {1998}

NCBI tax ID(s): 1525 (species)

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General

@ref: 4823

BacDive-ID: 16815

DSM-Number: 12797

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic

description: Moorella thermoacetica ET-5a is an anaerobe, thermophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 1525
  • Matching level: species

strain history

  • @ref: 4823
  • history: <- H. L. Drake, University of Bayreuth, Department of Ecological Microbiology, Bayreuth, Germany; ET-5a <- H. L. Drake and A. Goessner {1998}

doi: 10.13145/bacdive16815.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Moorellales
  • family: Moorellaceae
  • genus: Moorella
  • species: Moorella thermoacetica
  • full scientific name: Moorella thermoacetica (Fontaine et al. 1942) Collins et al. 1994
  • synonyms

    @refsynonym
    20215Moorella thermoautotrophica
    20215Clostridium thermoaceticum
    20215Clostridium thermautotrophicum
    20215Clostridium thermoautotrophicum
    20215Clostridium thermaceticum

@ref: 4823

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Thermoanaerobacteraceae

genus: Moorella

species: Moorella thermoacetica

full scientific name: Moorella thermoacetica (Fontaine et al. 1942) Collins et al. 1994

strain designation: ET-5a

type strain: no

Culture and growth conditions

culture medium

  • @ref: 4823
  • name: SPOROMUSA ACIDOVORANS MEDIUM (DSMZ Medium 311c)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/311c
  • composition: Name: SPOROMUSA ACIDOVORANS MEDIUM (DSMZ Medium 311c) Composition: D-Fructose 4.97512 g/l NaCl 2.23881 g/l Yeast extract 1.99005 g/l Casitone 1.99005 g/l Na2CO3 0.995025 g/l MgSO4 x 7 H2O 0.497512 g/l NH4Cl 0.497512 g/l K2HPO4 0.348259 g/l Na2S x 9 H2O 0.298507 g/l L-Cysteine HCl x H2O 0.298507 g/l CaCl2 x 2 H2O 0.248756 g/l KH2PO4 0.228856 g/l HCl 0.00248756 g/l FeSO4 x 7 H2O 0.00199005 g/l FeCl2 x 4 H2O 0.00149254 g/l NaOH 0.000497512 g/l Sodium resazurin 0.000497512 g/l CoCl2 x 6 H2O 0.000189055 g/l Pyridoxine hydrochloride 9.95025e-05 g/l MnCl2 x 4 H2O 9.95025e-05 g/l ZnCl2 6.96517e-05 g/l p-Aminobenzoic acid 4.97512e-05 g/l Calcium D-(+)-pantothenate 4.97512e-05 g/l Nicotinic acid 4.97512e-05 g/l Riboflavin 4.97512e-05 g/l Thiamine HCl 4.97512e-05 g/l (DL)-alpha-Lipoic acid 4.97512e-05 g/l Na2MoO4 x 2 H2O 3.58209e-05 g/l NiCl2 x 6 H2O 2.38806e-05 g/l Biotin 1.99005e-05 g/l Folic acid 1.99005e-05 g/l H3BO3 5.97015e-06 g/l Na2WO4 x 2 H2O 3.9801e-06 g/l Na2SeO3 x 5 H2O 2.98507e-06 g/l CuCl2 x 2 H2O 1.99005e-06 g/l Vitamin B12 9.95025e-07 g/l Distilled water

culture temp

  • @ref: 4823
  • growth: positive
  • type: growth
  • temperature: 55

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
4823anaerobe
69480anaerobe91.873

Isolation, sampling and environmental information

isolation

  • @ref: 4823
  • sample type: soil
  • country: Egypt
  • origin.country: EGY
  • continent: Africa

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_94.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15934;96_75;97_78;98_85;99_94&stattab=map
  • Last taxonomy: Moorella thermoacetica
  • 16S sequence: AJ242494
  • Sequence Identity:
  • Total samples: 595
  • soil counts: 87
  • aquatic counts: 257
  • animal counts: 219
  • plant counts: 32

Safety information

risk assessment

  • @ref: 4823
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4823
  • description: Moorella thermoacetica 16S rRNA gene, strain ET-5a
  • accession: AJ242494
  • length: 1557
  • database: nuccore
  • NCBI tax ID: 1525

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Moorella thermoacetica DSM 12797GCA_001874085contigncbi1525
66792Moorella thermoacetica strain DSM 127971525.7wgspatric1525
66792Moorella thermoacetica DSM 127972703719210draftimg1525

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno78no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes54.422no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)yes91.873no
69480spore-formingspore-formingAbility to form endo- or exosporesyes73.994no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no92.578yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cyes78.525yes
69480flagellatedmotile2+Ability to perform flagellated movementyes69.462no

External links

@ref: 4823

culture collection no.: DSM 12797

straininfo link

  • @ref: 85827
  • straininfo: 49361

literature

  • topic: Phylogeny
  • Pubmed-ID: 10543831
  • title: Thermicanus aegyptius gen. nov., sp. nov., isolated from oxic soil, a fermentative microaerophile that grows commensally with the thermophilic acetogen Moorella thermoacetica.
  • authors: Gossner AS, Devereux R, Ohnemuller N, Acker G, Stackebrandt E, Drake HL
  • journal: Appl Environ Microbiol
  • DOI: 10.1128/AEM.65.11.5124-5133.1999
  • year: 1999
  • mesh: Aerobiosis, Anaerobiosis, Cellobiose/metabolism, DNA, Ribosomal/genetics, Egypt, Fermentation, Genes, Bacterial, Gram-Positive Bacteria/*classification/*growth & development/ultrastructure, Hot Temperature, Microscopy, Electron, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, *Soil Microbiology
  • topic2: Stress

Reference

@idauthorscataloguedoi/urltitle
4823Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12797)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12797
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
85827Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49361.1StrainInfo: A central database for resolving microbial strain identifiers