Strain identifier

BacDive ID: 16811

Type strain: Yes

Species: Moorella thermoacetica

Strain Designation: Fontaine

Variant: Isotype of BacDive ID 16812

Strain history: CIP <- 2004, CCUG <- J.R. Andreesen <- L.G. Ljungdahl <- H.G. Wood <- H.A. Barker <- F. Fontaine

NCBI tax ID(s): 1525 (species)

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General

@ref: 265

BacDive-ID: 16811

DSM-Number: 521

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-positive, rod-shaped

description: Moorella thermoacetica Fontaine is an anaerobe, thermophilic, Gram-positive bacterium that was isolated from horse faeces.

NCBI tax id

  • NCBI tax id: 1525
  • Matching level: species

strain history

@refhistory
265<- J. R. Andreesen, Universität Göttingen, Germany <- L. G. Ljungdahl <- H. G. Wood <- H. A. Barker <- F. E. Fontaine, University of Wisconsin, Departments of Biochemistry and Agricultural Bacteriology, Madison, USA
67770E. Uematsu <-- ATCC 35608 <-- J. G. Zeikus strain Fontaine <-- E. McCoy <-- F. G. Fontaine.
123500CIP <- 2004, CCUG <- J.R. Andreesen <- L.G. Ljungdahl <- H.G. Wood <- H.A. Barker <- F. Fontaine

doi: 10.13145/bacdive16811.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Thermoanaerobacterales
  • family: Thermoanaerobacteraceae
  • genus: Moorella
  • species: Moorella thermoacetica
  • full scientific name: Moorella thermoacetica (Fontaine et al. 1942) Collins et al. 1994

  • synonyms

    @refsynonym
    20215Clostridium thermaceticum
    20215Moorella thermoautotrophica
    20215Clostridium thermoautotrophicum
    20215Clostridium thermoaceticum
    20215Clostridium thermautotrophicum

@ref: 265

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Thermoanaerobacteraceae

genus: Moorella

species: Moorella thermoacetica

full scientific name: Moorella thermoacetica (Fontaine et al. 1942) Collins et al. 1994

strain designation: Fontaine

variant: Isotype of BacDive ID 16812

type strain: yes

Morphology

cell morphology

  • @ref: 123500
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 123500

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
265MOORELLA MEDIUM (DSMZ Medium 60)yeshttps://mediadive.dsmz.de/medium/60Name: MOORELLA MEDIUM (DSMZ Medium 60) Composition: Glucose 17.9462 g/l K2HPO4 6.97906 g/l Yeast extract 4.98505 g/l Tryptone 4.98505 g/l KH2PO4 4.48654 g/l Na2CO3 1.99402 g/l (NH4)2SO4 0.498505 g/l L-Cysteine HCl x H2O 0.299103 g/l Na2S x 9 H2O 0.299103 g/l MgSO4 x 7 H2O 0.0997009 g/l Fe(NH4)2(SO4)2 0.0398804 g/l Na2MoO4 x 2 H2O 0.00239283 g/l Sodium resazurin 0.000498505 g/l Na2SeO3 x 5 H2O 0.000149551 g/l Distilled water
32808MEDIUM 224 - for anaerobic bacteria TGVyesDistilled water make up to (1000.000 ml);Glucose (10.000 g);Yeast extract (20.000 g);Resazurin (2.000 mg);Trypto casein soy broth (30.000 g);L-Cysteine (0.500 g);Hemin solution - M00149 (25.000 ml);Vitamins solution - M00850 (10.000 ml)
123500CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
265positivegrowth55thermophilic
32808positivegrowth55thermophilic
67770positivegrowth60thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
265anaerobe
123500anaerobe

compound production

@refcompound
265acetate kinase
265formate dehydrogenase
265formyltetrahydrofolate synthase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12350017108D-arabinose+degradation
12350015824D-fructose+degradation
12350017634D-glucose+degradation
12350065327D-xylose+degradation
12350017057cellobiose-degradation
12350017716lactose-degradation
12350017306maltose-degradation
12350017814salicin-degradation
12350017992sucrose-degradation
1235004853esculin-hydrolysis
12350017632nitrate+reduction
12350016301nitrite-reduction
12350017632nitrate+respiration

metabolite production

  • @ref: 123500
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueecactivity
67770acetate kinase2.7.2.1
123500oxidase-
123500beta-galactosidase3.2.1.23-
123500gelatinase-
123500catalase1.11.1.6-
123500urease3.5.1.5-

Isolation, sampling and environmental information

isolation

@refsample type
265horse faeces
67770Horse feces
123500Animal, Horse faeces

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Equidae (Horse)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_94.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15934;96_75;97_78;98_85;99_94&stattab=map
  • Last taxonomy: Moorella thermoacetica
  • 16S sequence: AB572912
  • Sequence Identity:
  • Total samples: 595
  • soil counts: 87
  • aquatic counts: 257
  • animal counts: 219
  • plant counts: 32

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
2651Risk group (German classification)
1235001Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Moorella thermoacetica strain DSM 521 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequence; and tRNA-Ile and tRNA-Ala genes, complete sequenceDQ192547522ena1525
20218Moorella thermoacetica gene for 16S ribosomal RNA, partial sequence, strain: JCM 9319AB5729121529ena1525
265Moorella thermoacetica strain DSM 521 16S ribosomal RNA gene, partial sequenceAY6566751430ena1525

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Moorella thermoacetica DSM 521GCA_001267405completencbi1525
66792Moorella thermoacetica strain ATCC 356081525.21wgspatric1525
66792Moorella thermoacetica strain DSM 5211525.3completepatric1525
66792Moorella thermoacetica DSM 5212834890540completeimg1525
66792Moorella thermoacetica DSM 5212627853541completeimg1525
66792Moorella thermoacetica DSM 5212596583675draftimg1525
67770Moorella thermoacetica ATCC 35608GCA_008121885contigncbi1525

GC content

  • @ref: 265
  • GC-content: 56.0
  • method: sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes88.536no
flagellatedno93.618no
gram-positiveno69.108no
anaerobicyes99.049no
aerobicno96.416yes
halophileno95.611no
spore-formingyes94.068no
thermophileyes100yes
glucose-utilyes86.314no
glucose-fermentno71.381no

External links

@ref: 265

culture collection no.: DSM 521, ATCC 35608, VPI 12954, JCM 9319, KCTC 5000, VPI 12984, CIP 108294

straininfo link

  • @ref: 85824
  • straininfo: 41191

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism18378590Old acetogens, new light.Drake HL, Gossner AS, Daniel SLAnn N Y Acad Sci10.1196/annals.1419.0162008Acetyl Coenzyme A/*metabolism, Carbon Dioxide/metabolism, Carbon Radioisotopes, Clostridium/classification/genetics/*metabolism, Energy Metabolism, Evolution, Molecular, Oxidation-Reduction, Phylogeny, WoodPhylogeny
Metabolism18854977Atypical one-carbon metabolism of an acetogenic and hydrogenogenic Moorella thermoacetica strain.Jiang B, Henstra AM, Paulo PL, Balk M, van Doesburg W, Stams AJArch Microbiol10.1007/s00203-008-0435-x2008Acetates/*metabolism, Bioreactors/microbiology, Carbon/*metabolism, Gram-Positive Bacteria/classification/genetics/*isolation & purification/*metabolism, Hydrogen/*metabolism, Methanol/metabolism, Molecular Sequence Data, PhylogenyPhylogeny
Genetics26450731Complete Genome Sequence of the Type Strain of the Acetogenic Bacterium Moorella thermoacetica DSM 521T.Poehlein A, Bengelsdorf FR, Esser C, Schiel-Bengelsdorf B, Daniel R, Durre PGenome Announc10.1128/genomeA.01159-152015

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
265Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 521)https://www.dsmz.de/collection/catalogue/details/culture/DSM-521
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32808Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5900
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
85824Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID41191.1StrainInfo: A central database for resolving microbial strain identifiers
123500Curators of the CIPCollection of Institut Pasteur (CIP 108294)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108294