Strain identifier
BacDive ID: 16802
Type strain: 
Species: Thermanaeromonas toyohensis
Strain Designation: ToBE
Strain history: K. Mori strain ToBE.
NCBI tax ID(s): 698762 (strain), 161154 (species)
version 8.1 (current version)
General
@ref: 5376
BacDive-ID: 16802
DSM-Number: 14490
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic
description: Thermanaeromonas toyohensis ToBE is an anaerobe, thermophilic bacterium that was isolated from geothermal aquifer at a depth of 550m.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 698762 | strain |
| 161154 | species |
strain history
| @ref | history |
|---|---|
| 5376 | <- K. Mori, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan; ToBE <- K. Mori {2001} |
| 67770 | K. Mori strain ToBE. |
doi: 10.13145/bacdive16802.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Thermanaeromonas
- species: Thermanaeromonas toyohensis
- full scientific name: Thermanaeromonas toyohensis Mori et al. 2002
@ref: 5376
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Thermanaeromonas
species: Thermanaeromonas toyohensis
full scientific name: Thermanaeromonas toyohensis Mori et al. 2002
strain designation: ToBE
type strain: yes
Morphology
multimedia
- @ref: 66793
- multimedia content: EM_DSM_14490_1.jpg
- caption: electron microscopic image
- intellectual property rights: © HZI/Manfred Rohde
Culture and growth conditions
culture medium
- @ref: 5376
- name: THERMOANAEROMONAS MEDIUM (DSMZ Medium 963)
- growth: yes
- link: https://mediadive.dsmz.de/medium/963
- composition: Name: THERMOANAEROMONAS MEDIUM (DSMZ Medium 963) Composition: NaHCO3 4.9456 g/l Na2S2O3 x 5 H2O 1.22651 g/l K2HPO4 0.771513 g/l KH2PO4 0.74184 g/l Yeast extract 0.49456 g/l D-Glucose 0.49456 g/l NH4Cl 0.49456 g/l L-Cysteine HCl x H2O 0.24728 g/l MgSO4 x 7 H2O 0.24728 g/l Nitrilotriacetic acid 0.126607 g/l Na3-EDTA 0.0395648 g/l NaCl 0.0098912 g/l FeSO4 x 7 H2O 0.0098912 g/l FeCl2 x 4 H2O 0.0098912 g/l CaCl2 x 2 H2O 0.00098912 g/l MnCl2 x 4 H2O 0.00098912 g/l ZnCl2 0.00098912 g/l NiCl2 x 6 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l Na2WO4 x 2 H2O 0.000395648 g/l Na2SeO3 x 5 H2O 0.000296736 g/l CoCl2 x 6 H2O 0.000296736 g/l Na2MoO4 x 2 H2O 0.000296736 g/l CuCl2 0.000197824 g/l H3BO3 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Vitamin B12 9.8912e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 5376 | positive | growth | 70 | thermophilic |
| 67770 | positive | growth | 70 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 5376
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 5376 | geothermal aquifer at a depth of 550m | Hokkaido, Toyoha Mine | Japan | JPN | Asia |
| 67770 | Geothermal water at Toyoha Mine | Hokkaido | Japan | JPN | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #Groundwater
Safety information
risk assessment
- @ref: 5376
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 5376
- description: Thermoanaerobacter sp. ToBE gene for 16S rRNA
- accession: AB062280
- length: 1506
- database: ena
- NCBI tax ID: 698762
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermanaeromonas toyohensis ToBE | GCA_900176005 | chromosome | ncbi | 698762 |
| 66792 | Thermanaeromonas toyohensis ToBE, DSM 14490 | 2503538017 | complete | img | 698762 |
GC content
- @ref: 5376
- GC-content: 49.6
- method: high performance liquid chromatography (HPLC)
External links
@ref: 5376
culture collection no.: DSM 14490, JCM 11376, NBRC 101528
straininfo link
- @ref: 85817
- straininfo: 100998
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 12361273 | Thermanaeromonas toyohensis gen. nov., sp. nov., a novel thermophilic anaerobe isolated from a subterranean vein in the Toyoha Mines. | Mori K, Hanada S, Maruyama A, Marumo K | Int J Syst Evol Microbiol | 10.1099/00207713-52-5-1675 | 2002 | Bacteria, Anaerobic/*classification/genetics/*isolation & purification/metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fresh Water/microbiology, Hot Temperature, Hydrogen-Ion Concentration, Japan, Microscopy, Electron, Mining, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as Topic | Stress |
| Phylogeny | 17220449 | Desulfovirgula thermocuniculi gen. nov., sp. nov., a thermophilic sulfate-reducer isolated from a geothermal underground mine in Japan. | Kaksonen AH, Spring S, Schumann P, Kroppenstedt RM, Puhakka JA | Int J Syst Evol Microbiol | 10.1099/ijs.0.64655-0 | 2007 | DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Gram-Positive Endospore-Forming Bacteria/chemistry/*classification/genetics/isolation & purification, *Hot Temperature, Japan, *Mining, Molecular Sequence Data, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfates/*metabolism | Stress |
| Phylogeny | 26541283 | Thermanaeromonas burensis sp. nov., a thermophilic anaerobe isolated from a subterranean clay environment. | Gam ZBA, Daumas S, Casalot L, Bartoli-Joseph M, Necib S, Linard Y, Labat M | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000739 | 2015 | Aluminum Silicates, Bacteria, Anaerobic/classification/genetics/isolation & purification, Base Composition, Clay, DNA, Bacterial/genetics, Fatty Acids/chemistry, Firmicutes/*classification/genetics/isolation & purification, France, Fresh Water/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Enzymology |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 5376 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14490) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14490 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66793 | Mukherjee et al. | 10.1038/nbt.3886 | GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life | 35: 676-683 2017 | 28604660 | |
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 85817 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID100998.1 | StrainInfo: A central database for resolving microbial strain identifiers |