Strain identifier

BacDive ID: 16801

Type strain: Yes

Species: Tepidanaerobacter acetatoxydans

Strain Designation: Re1

Strain history: A. Schnürer Re1.

NCBI tax ID(s): 499229 (species)

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General

@ref: 15965

BacDive-ID: 16801

DSM-Number: 21804

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Tepidanaerobacter acetatoxydans Re1 is an anaerobe, mesophilic bacterium that was isolated from silage of alfalfa in a stirred laboratory reactor.

NCBI tax id

  • NCBI tax id: 499229
  • Matching level: species

strain history

@refhistory
15965<- A. Schnürer, Swedish Univ. Agric. Sci., Uppsala, Sweden; Re1
67770A. Schnürer Re1.

doi: 10.13145/bacdive16801.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Thermosediminibacterales
  • family: Tepidanaerobacteraceae
  • genus: Tepidanaerobacter
  • species: Tepidanaerobacter acetatoxydans
  • full scientific name: Tepidanaerobacter acetatoxydans Westerholm et al. 2011

@ref: 15965

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Tepidanaerobacteraceae

genus: Tepidanaerobacter

species: Tepidanaerobacter acetatoxydans

full scientific name: Tepidanaerobacter acetatoxydans Westerholm et al. 2011

strain designation: Re1

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 15965
  • name: TEPIDANAEROBACTER ACETATOXYDANS MEDIUM (DSMZ Medium 1301)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1301
  • composition: Name: TEPIDANAEROBACTER ACETATOXYDANS MEDIUM (DSMZ Medium 1301) Composition: D-Glucose 1.8 g/l Na2CO3 1.5 g/l L-Cysteine HCl x H2O 0.5 g/l Na2HPO4 0.43 g/l KH2PO4 0.41 g/l Na2S x 9 H2O 0.3 g/l NH4Cl 0.3 g/l NaCl 0.3 g/l Yeast extract 0.2 g/l CaCl2 x 2 H2O 0.11 g/l MgCl2 x 6 H2O 0.1 g/l FeCl2 x 4 H2O 0.002 g/l Na2-EDTA 0.0005 g/l NaOH 0.0005 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Pyridoxine hydrochloride 2e-05 g/l (DL)-alpha-Lipoic acid 1e-05 g/l Riboflavin 1e-05 g/l p-Aminobenzoic acid 1e-05 g/l Calcium D-(+)-pantothenate 1e-05 g/l Thiamine HCl 1e-05 g/l AlCl3 1e-05 g/l Nicotinic acid 1e-05 g/l H3BO3 6e-06 g/l Folic acid 4e-06 g/l Na2WO4 x 2 H2O 4e-06 g/l Biotin 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 2e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15965positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 15965
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15965silage of alfalfa in a stirred laboratory reactorUppsalaSwedenSWEEurope
67770Two ammonium-enriched mesophilic methanogenic processes

isolation source categories

Cat1Cat2Cat3
#Engineered#Bioreactor
#Engineered#Laboratory
#Host#Plants#Herbaceous plants (Grass,Crops)

taxonmaps

  • @ref: 69479
  • File name: preview.99_6228.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_560;96_2965;97_3646;98_4614;99_6228&stattab=map
  • Last taxonomy: Tepidanaerobacter acetatoxydans subclade
  • 16S sequence: EU386163
  • Sequence Identity:
  • Total samples: 4897
  • soil counts: 174
  • aquatic counts: 806
  • animal counts: 3804
  • plant counts: 113

Sequence information

16S sequences

  • @ref: 15965
  • description: Tepidanaerobacter acetatoxydans Re1 16S ribosomal RNA gene, partial sequence
  • accession: EU386163
  • length: 1458
  • database: ena
  • NCBI tax ID: 1209989

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Tepidanaerobacter acetatoxydans Re1GCA_000213235completencbi1209989
66792Tepidanaerobacter acetatoxydans Re1GCA_000328765completencbi1209989
66792Tepidanaerobacter acetatoxydans Re11209989.3completepatric1209989
66792Tepidanaerobacter acetatoxydans Re12540341113completeimg1209989

GC content

  • @ref: 15965
  • GC-content: 38.4
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes77.233no
flagellatedno86.326no
gram-positiveno74.876no
anaerobicyes98.97no
halophileno88.778no
spore-formingyes56.68no
thermophileyes95.716no
glucose-utilyes90.969no
aerobicno93.868yes
glucose-fermentyes66.05no

External links

@ref: 15965

culture collection no.: DSM 21804, JCM 16047

literature

  • topic: Phylogeny
  • Pubmed-ID: 21498020
  • title: Tepidanaerobacter acetatoxydans sp. nov., an anaerobic, syntrophic acetate-oxidizing bacterium isolated from two ammonium-enriched mesophilic methanogenic processes.
  • authors: Westerholm M, Roos S, Schnurer A
  • journal: Syst Appl Microbiol
  • DOI: 10.1016/j.syapm.2010.11.018
  • year: 2011
  • mesh: Acetates/metabolism, Anaerobiosis, Base Composition, Carbon/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Formate-Tetrahydrofolate Ligase/genetics, Gram-Positive Bacteria/*classification/genetics/*isolation & purification/physiology, Hot Temperature, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Phylogeny, Quaternary Ammonium Compounds/metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
15965Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21804)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21804
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta