Strain identifier

BacDive ID: 16797

Type strain: Yes

Species: Caldanaerobacter uzonensis

Strain Designation: K67

Strain history: <- I. V. Kozina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; K67 <- E. A. Bonch-Osmolovskaya {1998}

NCBI tax ID(s): 447594 (species)

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General

@ref: 7777

BacDive-ID: 16797

DSM-Number: 18923

keywords: 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, rod-shaped

description: Caldanaerobacter uzonensis K67 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from terrestrial hot spring.

NCBI tax id

  • NCBI tax id: 447594
  • Matching level: species

strain history

  • @ref: 7777
  • history: <- I. V. Kozina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; K67 <- E. A. Bonch-Osmolovskaya {1998}

doi: 10.13145/bacdive16797.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Thermoanaerobacterales
  • family: Thermoanaerobacteraceae
  • genus: Caldanaerobacter
  • species: Caldanaerobacter uzonensis
  • full scientific name: Caldanaerobacter uzonensis Kozina et al. 2010

@ref: 7777

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Thermoanaerobacteraceae

genus: Caldanaerobacter

species: Caldanaerobacter uzonensis

full scientific name: Caldanaerobacter uzonensis Kozina et al. 2010

strain designation: K67

type strain: yes

Morphology

cell morphology

  • @ref: 29399
  • cell length: 2.5 µm
  • cell width: 0.4 µm
  • cell shape: rod-shaped

Culture and growth conditions

culture medium

  • @ref: 7777
  • name: CARBOXYDOTHERMUS MEDIUM (HETEROTROPH) (DSMZ Medium 508)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/508
  • composition: Name: CARBOXYDOTHERMUS MEDIUM (HETEROTROPH) (DSMZ Medium 508; with strain-specific modifications) Composition: D-Glucose 1.97628 g/l Na2CO3 0.988142 g/l MgCl2 x 6 H2O 0.513834 g/l KCl 0.326087 g/l NH4Cl 0.326087 g/l KH2PO4 0.326087 g/l Na2S x 9 H2O 0.296443 g/l CaCl2 x 2 H2O 0.286561 g/l Yeast extract 0.0494071 g/l Na2-EDTA 0.00494071 g/l H3BO3 0.00296443 g/l CoCl2 x 6 H2O 0.00197628 g/l FeSO4 x 7 H2O 0.00197628 g/l ZnSO4 x 7 H2O 0.000988142 g/l Sodium resazurin 0.000494071 g/l MnCl2 x 4 H2O 0.000296443 g/l Pyridoxine hydrochloride 0.000296443 g/l Na2MoO4 x 2 H2O 0.000296443 g/l Nicotinic acid 0.000197628 g/l Thiamine-HCl x 2 H2O 0.000197628 g/l NiCl2 x 6 H2O 0.000197628 g/l CuCl2 x 2 H2O 9.88142e-05 g/l Calcium pantothenate 9.88142e-05 g/l Vitamin B12 9.88142e-05 g/l p-Aminobenzoic acid 7.90514e-05 g/l Calcium D-(+)-pantothenate 4.94071e-05 g/l Riboflavin 4.94071e-05 g/l (DL)-alpha-Lipoic acid 4.94071e-05 g/l Thiamine HCl 4.94071e-05 g/l Folic acid 1.97628e-05 g/l D-(+)-biotin 1.97628e-05 g/l Biotin 1.97628e-05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
7777positivegrowth70thermophilic
29399positivegrowth50-75thermophilic
29399positiveoptimum69thermophilic

culture pH

@refabilitytypepH
29399positivegrowth4.8-8
29399positiveoptimum6.8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
7777anaerobe
29399anaerobe

spore formation

  • @ref: 29399
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
29399NaClpositivegrowth0-2 %
29399NaClpositiveoptimum0.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2939922599arabinose+carbon source
2939937684mannose+carbon source
2939917992sucrose+carbon source
2939918222xylose+carbon source

metabolite production

  • @ref: 29399
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

Isolation, sampling and environmental information

isolation

  • @ref: 7777
  • sample type: terrestrial hot spring
  • geographic location: Kamchatka Peninsula, Uzon Caldera (N 54° 49' E 160° 01')
  • country: Russia
  • origin.country: RUS
  • continent: Asia
  • latitude: 54.8167
  • longitude: 160.017

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Environmental#Terrestrial
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_46468.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_101;96_21189;97_26421;98_33594;99_46468&stattab=map
  • Last taxonomy: Caldanaerobacter uzonensis subclade
  • 16S sequence: EF195126
  • Sequence Identity:
  • Total samples: 33
  • soil counts: 1
  • aquatic counts: 26
  • animal counts: 6

Safety information

risk assessment

  • @ref: 7777
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7777
  • description: Caldanaerobacter uzonensis strain K67 16S ribosomal RNA gene, partial sequence
  • accession: EF195126
  • length: 1417
  • database: ena
  • NCBI tax ID: 447594

GC content

@refGC-contentmethod
777734.2±0.5thermal denaturation, midpoint method (Tm)
2939934.2

External links

@ref: 7777

culture collection no.: DSM 18923, VKM B-2408

straininfo link

  • @ref: 85813
  • straininfo: 405663

literature

  • topic: Phylogeny
  • Pubmed-ID: 19671728
  • title: Caldanaerobacter uzonensis sp. nov., an anaerobic, thermophilic, heterotrophic bacterium isolated from a hot spring.
  • authors: Kozina IV, Kublanov IV, Kolganova TV, Chernyh NA, Bonch-Osmolovskaya EA
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.012328-0
  • year: 2009
  • mesh: Bacteria, Anaerobic/classification/*genetics/isolation & purification/metabolism, Cell Size, Hexoses/metabolism, Hot Springs/*microbiology, Hydrogen-Ion Concentration, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7777Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18923)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18923
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29399Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2580628776041
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
85813Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID405663.1StrainInfo: A central database for resolving microbial strain identifiers