Strain identifier
BacDive ID: 16797
Type strain:
Species: Caldanaerobacter uzonensis
Strain Designation: K67
Strain history: <- I. V. Kozina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; K67 <- E. A. Bonch-Osmolovskaya {1998}
NCBI tax ID(s): 447594 (species)
General
@ref: 7777
BacDive-ID: 16797
DSM-Number: 18923
keywords: 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, rod-shaped
description: Caldanaerobacter uzonensis K67 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from terrestrial hot spring.
NCBI tax id
- NCBI tax id: 447594
- Matching level: species
strain history
- @ref: 7777
- history: <- I. V. Kozina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; K67 <- E. A. Bonch-Osmolovskaya {1998}
doi: 10.13145/bacdive16797.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Caldanaerobacter
- species: Caldanaerobacter uzonensis
- full scientific name: Caldanaerobacter uzonensis Kozina et al. 2010
@ref: 7777
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Caldanaerobacter
species: Caldanaerobacter uzonensis
full scientific name: Caldanaerobacter uzonensis Kozina et al. 2010
strain designation: K67
type strain: yes
Morphology
cell morphology
- @ref: 29399
- cell length: 2.5 µm
- cell width: 0.4 µm
- cell shape: rod-shaped
Culture and growth conditions
culture medium
- @ref: 7777
- name: CARBOXYDOTHERMUS MEDIUM (HETEROTROPH) (DSMZ Medium 508)
- growth: yes
- link: https://mediadive.dsmz.de/medium/508
- composition: Name: CARBOXYDOTHERMUS MEDIUM (HETEROTROPH) (DSMZ Medium 508; with strain-specific modifications) Composition: D-Glucose 1.97628 g/l Na2CO3 0.988142 g/l MgCl2 x 6 H2O 0.513834 g/l KCl 0.326087 g/l NH4Cl 0.326087 g/l KH2PO4 0.326087 g/l Na2S x 9 H2O 0.296443 g/l CaCl2 x 2 H2O 0.286561 g/l Yeast extract 0.0494071 g/l Na2-EDTA 0.00494071 g/l H3BO3 0.00296443 g/l CoCl2 x 6 H2O 0.00197628 g/l FeSO4 x 7 H2O 0.00197628 g/l ZnSO4 x 7 H2O 0.000988142 g/l Sodium resazurin 0.000494071 g/l MnCl2 x 4 H2O 0.000296443 g/l Pyridoxine hydrochloride 0.000296443 g/l Na2MoO4 x 2 H2O 0.000296443 g/l Nicotinic acid 0.000197628 g/l Thiamine-HCl x 2 H2O 0.000197628 g/l NiCl2 x 6 H2O 0.000197628 g/l CuCl2 x 2 H2O 9.88142e-05 g/l Calcium pantothenate 9.88142e-05 g/l Vitamin B12 9.88142e-05 g/l p-Aminobenzoic acid 7.90514e-05 g/l Calcium D-(+)-pantothenate 4.94071e-05 g/l Riboflavin 4.94071e-05 g/l (DL)-alpha-Lipoic acid 4.94071e-05 g/l Thiamine HCl 4.94071e-05 g/l Folic acid 1.97628e-05 g/l D-(+)-biotin 1.97628e-05 g/l Biotin 1.97628e-05 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
7777 | positive | growth | 70 | thermophilic |
29399 | positive | growth | 50-75 | thermophilic |
29399 | positive | optimum | 69 | thermophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
29399 | positive | growth | 4.8-8 |
29399 | positive | optimum | 6.8 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
7777 | anaerobe |
29399 | anaerobe |
spore formation
- @ref: 29399
- spore formation: yes
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
29399 | NaCl | positive | growth | 0-2 % |
29399 | NaCl | positive | optimum | 0.5 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
29399 | 22599 | arabinose | + | carbon source |
29399 | 37684 | mannose | + | carbon source |
29399 | 17992 | sucrose | + | carbon source |
29399 | 18222 | xylose | + | carbon source |
metabolite production
- @ref: 29399
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: yes
Isolation, sampling and environmental information
isolation
- @ref: 7777
- sample type: terrestrial hot spring
- geographic location: Kamchatka Peninsula, Uzon Caldera (N 54° 49' E 160° 01')
- country: Russia
- origin.country: RUS
- continent: Asia
- latitude: 54.8167
- longitude: 160.017
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Thermal spring |
#Environmental | #Terrestrial | |
#Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_46468.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_101;96_21189;97_26421;98_33594;99_46468&stattab=map
- Last taxonomy: Caldanaerobacter uzonensis subclade
- 16S sequence: EF195126
- Sequence Identity:
- Total samples: 33
- soil counts: 1
- aquatic counts: 26
- animal counts: 6
Safety information
risk assessment
- @ref: 7777
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 7777
- description: Caldanaerobacter uzonensis strain K67 16S ribosomal RNA gene, partial sequence
- accession: EF195126
- length: 1417
- database: ena
- NCBI tax ID: 447594
GC content
@ref | GC-content | method |
---|---|---|
7777 | 34.2±0.5 | thermal denaturation, midpoint method (Tm) |
29399 | 34.2 |
External links
@ref: 7777
culture collection no.: DSM 18923, VKM B-2408
straininfo link
- @ref: 85813
- straininfo: 405663
literature
- topic: Phylogeny
- Pubmed-ID: 19671728
- title: Caldanaerobacter uzonensis sp. nov., an anaerobic, thermophilic, heterotrophic bacterium isolated from a hot spring.
- authors: Kozina IV, Kublanov IV, Kolganova TV, Chernyh NA, Bonch-Osmolovskaya EA
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.012328-0
- year: 2009
- mesh: Bacteria, Anaerobic/classification/*genetics/isolation & purification/metabolism, Cell Size, Hexoses/metabolism, Hot Springs/*microbiology, Hydrogen-Ion Concentration, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
7777 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18923) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-18923 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
29399 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25806 | 28776041 | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
85813 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID405663.1 | StrainInfo: A central database for resolving microbial strain identifiers |