Strain identifier

BacDive ID: 16796

Type strain: Yes

Species: Caldanaerobacter subterraneus subsp. tengcongensis

Strain Designation: MB4

Strain history: Y. Ma MB4.

NCBI tax ID(s): 119072 (subspecies)

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General

@ref: 5778

BacDive-ID: 16796

DSM-Number: 15242

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic

description: Caldanaerobacter subterraneus subsp. tengcongensis MB4 is an anaerobe, thermophilic bacterium that was isolated from mixed water and sediment sample.

NCBI tax id

  • NCBI tax id: 119072
  • Matching level: subspecies

strain history

@refhistory
5778<- JCM; MB4
67770Y. Ma MB4.

doi: 10.13145/bacdive16796.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Thermoanaerobacterales
  • family: Thermoanaerobacteraceae
  • genus: Caldanaerobacter
  • species: Caldanaerobacter subterraneus subsp. tengcongensis
  • full scientific name: Caldanaerobacter subterraneus subsp. tengcongensis (Xue et al. 2001) Fardeau et al. 2004

  • synonyms

    • @ref: 20215
    • synonym: Thermoanaerobacter tengcongensis

@ref: 5778

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Thermoanaerobacteraceae

genus: Caldanaerobacter

species: Caldanaerobacter subterraneus subsp. tengcongensis

full scientific name: Caldanaerobacter subterraneus subsp. tengcongensis (Xue et al. 2001) Fardeau et al. 2004

strain designation: MB4

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 5778
  • name: THERMOANAEROBACTER KREATINOPHILUS MEDIUM (DSMZ Medium 965)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/965
  • composition: Name: THERMOANAEROBACTER KREATINOPHILUS MEDIUM (DSMZ Medium 965) Composition: Starch 9.90099 g/l Na2S2O3 x 5 H2O 4.9505 g/l Tryptone 1.9802 g/l NH4Cl 0.990099 g/l Yeast extract 0.990099 g/l MgCl2 x 6 H2O 0.49505 g/l L-Cysteine HCl x H2O 0.49505 g/l KH2PO4 0.29703 g/l K2HPO4 0.29703 g/l KCl 0.19802 g/l MgSO4 x 7 H2O 0.029703 g/l Nitrilotriacetic acid 0.0148515 g/l NaCl 0.00990099 g/l MnSO4 x H2O 0.00495049 g/l CoSO4 x 7 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.00178218 g/l CaCl2 x 2 H2O 0.000990099 g/l FeSO4 x 7 H2O 0.000990099 g/l Sodium resazurin 0.00049505 g/l NiCl2 x 6 H2O 0.00029703 g/l AlK(SO4)2 x 12 H2O 0.00019802 g/l Na2MoO4 x 2 H2O 9.90099e-05 g/l CuSO4 x 5 H2O 9.90099e-05 g/l H3BO3 9.90099e-05 g/l Na2WO4 x 2 H2O 3.9604e-06 g/l Na2SeO3 x 5 H2O 2.9703e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
5778positivegrowth75thermophilic
67770positivegrowth75thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 5778
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5778mixed water and sediment sampleYunnan Province, Tengcong, hot springChinaCHNAsia
67770Hot springTengcongChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_3868.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_101;96_976;97_1136;98_1355;99_3868&stattab=map
  • Last taxonomy: Caldanaerobacter
  • 16S sequence: AF209708
  • Sequence Identity:
  • Total samples: 3
  • aquatic counts: 2
  • animal counts: 1

Safety information

risk assessment

  • @ref: 5778
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
5778Thermoanaerobacter tengcongensis 16S ribosomal RNA gene, partial sequenceAF2097081526ena119072
67770Caldanaerobacter subterraneus subsp. tengcongensis gene for 16S ribosomal RNA, partial sequenceAB5972591496ena119072

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Caldanaerobacter subterraneus subsp. tengcongensis MB4GCA_000007085completencbi273068
66792Caldanaerobacter subterraneus subsp. tengcongensis MB4GCA_024807135contigncbi273068

GC content

@refGC-contentmethod
577837sequence analysis
6777033thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes81.831no
flagellatedno85.627no
gram-positiveno64.656no
anaerobicyes99.219yes
halophileno97.056no
spore-formingyes88.816no
glucose-utilyes92.688no
aerobicno98.518yes
thermophileyes100yes
glucose-fermentyes53.45no

External links

@ref: 5778

culture collection no.: DSM 15242, CGMCC AS 1.2430, JCM 11007, CGMCC 1.2430, NBRC 100824

straininfo link

  • @ref: 85812
  • straininfo: 101000

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11491330Thermoanaerobacter tengcongensis sp. nov., a novel anaerobic, saccharolytic, thermophilic bacterium isolated from a hot spring in Tengcong, China.Xue Y, Xu Y, Liu Y, Ma Y, Zhou PInt J Syst Evol Microbiol10.1099/00207713-51-4-13352001Anaerobiosis, Bacillaceae/*classification/genetics/*isolation & purification/metabolism, Base Composition, Base Sequence, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fresh Water/microbiology, Hot Temperature, Microscopy, Electron, Molecular Sequence Data, Phylogeny, Species SpecificityStress
Phylogeny15023962Isolation from oil reservoirs of novel thermophilic anaerobes phylogenetically related to Thermoanaerobacter subterraneus: reassignment of T. subterraneus, Thermoanaerobacter yonseiensis, Thermoanaerobacter tengcongensis and Carboxydibrachium pacificum to Caldanaerobacter subterraneus gen. nov., sp. nov., comb. nov. as four novel subspecies.Fardeau ML, Salinas MB, L'Haridon S, Jeanthon C, Verhe F, Cayol JL, Patel BKC, Garcia JL, Ollivier BInt J Syst Evol Microbiol10.1099/ijs.0.02711-02004Anaerobiosis, Australia, Bacteria, Anaerobic/*classification/isolation & purification, Base Composition, Base Sequence, Clostridium/*classification/genetics/isolation & purification, DNA Primers, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fossil Fuels/*microbiology, France, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/geneticsEnzymology

Reference

@idauthorscataloguedoi/urltitle
5778Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15242)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15242
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
85812Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID101000.1StrainInfo: A central database for resolving microbial strain identifiers