Strain identifier
BacDive ID: 16796
Type strain: 
Species: Caldanaerobacter subterraneus subsp. tengcongensis
Strain Designation: MB4
Strain history: Y. Ma MB4.
NCBI tax ID(s): 119072 (subspecies)
version 9.3 (current version)
General
@ref: 5778
BacDive-ID: 16796
DSM-Number: 15242
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic
description: Caldanaerobacter subterraneus subsp. tengcongensis MB4 is an anaerobe, thermophilic bacterium that was isolated from mixed water and sediment sample.
NCBI tax id
- NCBI tax id: 119072
- Matching level: subspecies
strain history
| @ref | history |
|---|---|
| 5778 | <- JCM; MB4 |
| 67770 | Y. Ma MB4. |
doi: 10.13145/bacdive16796.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Caldanaerobacter
- species: Caldanaerobacter subterraneus subsp. tengcongensis
- full scientific name: Caldanaerobacter subterraneus subsp. tengcongensis (Xue et al. 2001) Fardeau et al. 2004
synonyms
- @ref: 20215
- synonym: Thermoanaerobacter tengcongensis
@ref: 5778
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Caldanaerobacter
species: Caldanaerobacter subterraneus subsp. tengcongensis
full scientific name: Caldanaerobacter subterraneus subsp. tengcongensis (Xue et al. 2001) Fardeau et al. 2004
strain designation: MB4
type strain: yes
Culture and growth conditions
culture medium
- @ref: 5778
- name: THERMOANAEROBACTER KREATINOPHILUS MEDIUM (DSMZ Medium 965)
- growth: yes
- link: https://mediadive.dsmz.de/medium/965
- composition: Name: THERMOANAEROBACTER KREATINOPHILUS MEDIUM (DSMZ Medium 965) Composition: Starch 9.90099 g/l Na2S2O3 x 5 H2O 4.9505 g/l Tryptone 1.9802 g/l NH4Cl 0.990099 g/l Yeast extract 0.990099 g/l MgCl2 x 6 H2O 0.49505 g/l L-Cysteine HCl x H2O 0.49505 g/l KH2PO4 0.29703 g/l K2HPO4 0.29703 g/l KCl 0.19802 g/l MgSO4 x 7 H2O 0.029703 g/l Nitrilotriacetic acid 0.0148515 g/l NaCl 0.00990099 g/l MnSO4 x H2O 0.00495049 g/l CoSO4 x 7 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.00178218 g/l CaCl2 x 2 H2O 0.000990099 g/l FeSO4 x 7 H2O 0.000990099 g/l Sodium resazurin 0.00049505 g/l NiCl2 x 6 H2O 0.00029703 g/l AlK(SO4)2 x 12 H2O 0.00019802 g/l Na2MoO4 x 2 H2O 9.90099e-05 g/l CuSO4 x 5 H2O 9.90099e-05 g/l H3BO3 9.90099e-05 g/l Na2WO4 x 2 H2O 3.9604e-06 g/l Na2SeO3 x 5 H2O 2.9703e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 5778 | positive | growth | 75 |
| 67770 | positive | growth | 75 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 5778 | anaerobe | |
| 125438 | anaerobe | 94.823 |
| 125439 | anaerobe | 99.9 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 5778 | mixed water and sediment sample | Yunnan Province, Tengcong, hot spring | China | CHN | Asia |
| 67770 | Hot spring | Tengcong | China | CHN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_3868.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_101;96_976;97_1136;98_1355;99_3868&stattab=map
- Last taxonomy: Caldanaerobacter
- 16S sequence: AF209708
- Sequence Identity:
- Total samples: 3
- aquatic counts: 2
- animal counts: 1
Safety information
risk assessment
- @ref: 5778
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 5778 | Thermoanaerobacter tengcongensis 16S ribosomal RNA gene, partial sequence | AF209708 | 1526 | nuccore | 119072 |
| 67770 | Caldanaerobacter subterraneus subsp. tengcongensis gene for 16S ribosomal RNA, partial sequence | AB597259 | 1496 | nuccore | 119072 |
| 124043 | Caldanaerobacter subterraneus subsp. tengcongensis partial 16S rRNA gene, strain DSM 15242, clone 1 | LN831306 | 1707 | nuccore | 119072 |
| 124043 | Caldanaerobacter subterraneus subsp. tengcongensis partial 16S rRNA gene, strain DSM 15242, clone 2 | LN831307 | 1643 | nuccore | 119072 |
| 124043 | Caldanaerobacter subterraneus subsp. tengcongensis partial 16S rRNA gene, strain DSM 15242, clone 3 | LN831308 | 1628 | nuccore | 119072 |
| 124043 | Caldanaerobacter subterraneus subsp. tengcongensis partial 16S rRNA gene, strain DSM 15242, clone 4 | LN831309 | 1607 | nuccore | 119072 |
| 124043 | Caldanaerobacter subterraneus subsp. tengcongensis partial 16S rRNA gene, strain DSM 15242, clone 5 | LN831310 | 1605 | nuccore | 119072 |
| 124043 | Caldanaerobacter subterraneus subsp. tengcongensis partial 16S rRNA gene, strain DSM 15242, clone 6 | LN831311 | 1668 | nuccore | 119072 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | GCA_000007085 | complete | ncbi | 273068 |
| 66792 | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | GCA_024807135 | contig | ncbi | 273068 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 5778 | 37 | sequence analysis |
| 67770 | 33 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 55.691 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 94.823 | yes |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 96.266 | yes |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 67.857 | no |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | yes | 73.42 | yes |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | yes | 73.451 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | yes | 67.6 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | yes | 68.5 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | variable | 73.9 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | anaerobe | 99.9 |
External links
@ref: 5778
culture collection no.: DSM 15242, CGMCC AS 1.2430, JCM 11007, CGMCC 1.2430, NBRC 100824
straininfo link
- @ref: 85812
- straininfo: 101000
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 11491330 | Thermoanaerobacter tengcongensis sp. nov., a novel anaerobic, saccharolytic, thermophilic bacterium isolated from a hot spring in Tengcong, China. | Xue Y, Xu Y, Liu Y, Ma Y, Zhou P | Int J Syst Evol Microbiol | 10.1099/00207713-51-4-1335 | 2001 | Anaerobiosis, Bacillaceae/*classification/genetics/*isolation & purification/metabolism, Base Composition, Base Sequence, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fresh Water/microbiology, Hot Temperature, Microscopy, Electron, Molecular Sequence Data, Phylogeny, Species Specificity | Stress |
| Phylogeny | 15023962 | Isolation from oil reservoirs of novel thermophilic anaerobes phylogenetically related to Thermoanaerobacter subterraneus: reassignment of T. subterraneus, Thermoanaerobacter yonseiensis, Thermoanaerobacter tengcongensis and Carboxydibrachium pacificum to Caldanaerobacter subterraneus gen. nov., sp. nov., comb. nov. as four novel subspecies. | Fardeau ML, Salinas MB, L'Haridon S, Jeanthon C, Verhe F, Cayol JL, Patel BKC, Garcia JL, Ollivier B | Int J Syst Evol Microbiol | 10.1099/ijs.0.02711-0 | 2004 | Anaerobiosis, Australia, Bacteria, Anaerobic/*classification/isolation & purification, Base Composition, Base Sequence, Clostridium/*classification/genetics/isolation & purification, DNA Primers, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fossil Fuels/*microbiology, France, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics | Enzymology |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 5778 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15242) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15242 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 85812 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID101000.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
| 124043 | Isabel Schober, Julia Koblitz | Data extracted from sequence databases, automatically matched based on designation and taxonomy | ||
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | |
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | https://github.com/GenomeNet/deepG | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 |