Strain identifier

BacDive ID: 167820

Type strain: Yes

Species: Azospirillum thermophilum

NCBI tax ID(s): 2202148 (species)

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General

@ref: 20215

BacDive-ID: 167820

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Azospirillum thermophilum KCTC 62259 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from Hot spring soil sample.

NCBI tax id

  • NCBI tax id: 2202148
  • Matching level: species

doi: 10.13145/bacdive167820.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Azospirillaceae
  • genus: Azospirillum
  • species: Azospirillum thermophilum
  • full scientific name: Azospirillum thermophilum Zhao et al. 2020

@ref: 20215

domain: Bacteria

phylum: Pseudomonadota

class: Alphaproteobacteria

order: Rhodospirillales

family: Azospirillaceae

genus: Azospirillum

species: Azospirillum thermophilum

full scientific name: Azospirillum thermophilum Zhao et al. 2020

type strain: yes

Morphology

cell morphology

  • @ref: 69521
  • gram stain: negative
  • cell length: 1.0-3.0 µm
  • cell width: 0.4-0.7 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 69521
  • colony color: white
  • colony shape: circular
  • incubation period: 3 days
  • medium used: T5 agar

Culture and growth conditions

culture medium

@refnamegrowth
69521T5 agaryes
69521T5yes

culture temp

@refgrowthtypetemperature
69521positivegrowth15.0-50.0
69521positiveoptimum50

culture pH

@refabilitytypepH
69521positivegrowth5.0-7.0
69521positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 69521
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
69521NaClpositivegrowth0.0-3.0 %(w/v)
69521NaClpositiveoptimum0 %(w/v)

observation

@refobservation
69521Positive for milk coagulation and milk peptonization.
69521The polar lipid profile consists of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmehtylethanolamine, two aminolipids and an unidentified phospholipid.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6952153423tween 40-degradation
6952153425tween 60-degradation
6952153426tween 80-degradation
6952129016arginine-hydrolysis
695215291gelatin-hydrolysis
6952128017starch-hydrolysis
69521581435-dehydro-D-gluconate+builds acid from
6952127613amygdalin+builds acid from
6952118305arbutin+builds acid from
6952117057cellobiose+builds acid from
6952128847D-fucose+builds acid from
6952162318D-lyxose+builds acid from
6952116443D-tagatose+builds acid from
6952165327D-xylose+builds acid from
695214853esculin+builds acid from
6952128260galactose+builds acid from
6952124265gluconate+builds acid from
6952117754glycerol+builds acid from
6952118287L-fucose+builds acid from
6952117716lactose+builds acid from
6952117306maltose+builds acid from
6952128053melibiose+builds acid from
69521506227N-acetylglucosamine+builds acid from
6952133942ribose+builds acid from
6952117814salicin+builds acid from
6952117992sucrose+builds acid from
6952127082trehalose+builds acid from
6952132528turanose+builds acid from
6952116651(S)-lactate+carbon source
69521370543-hydroxybutyrate+carbon source
6952130089acetate+carbon source
6952117925alpha-D-glucose+carbon source
6952173706bromosuccinate+carbon source
6952115824D-fructose+carbon source
6952128847D-fucose+carbon source
6952118024D-galacturonic acid+carbon source
6952118391D-gluconate+carbon source
6952115748D-glucuronate+carbon source
6952116899D-mannitol+carbon source
6952117814D-salicin+carbon source
6952117924D-sorbitol+carbon source
6952116551D-trehalose+carbon source
6952116865gamma-aminobutyric acid+carbon source
6952132323glucuronamide+carbon source
6952117464L-galactonic acid gamma-lactone+carbon source
6952115589L-malate+carbon source
69521320055methyl beta-D-glucopyranoside+carbon source
6952117268myo-inositol+carbon source
6952163154N-acetyl-beta-D-mannosamine+carbon source
69521506227N-acetyl-D-glucosamine+carbon source
6952117272propionate+carbon source
6952132528turanose+carbon source
6952153424tween 20+degradation
695214853esculin+hydrolysis
6952115428glycine+nitrogen source
6952117368hypoxanthine+nitrogen source
6952116977L-alanine+nitrogen source
6952116467L-arginine+nitrogen source
6952117196L-asparagine+nitrogen source
6952129985L-glutamate+nitrogen source
6952118019L-lysine+nitrogen source
6952117295L-phenylalanine+nitrogen source
6952117203L-proline+nitrogen source
6952116414L-valine+nitrogen source
6952127897tryptophan+nitrogen source
6952117632nitrate+reduction

metabolite production

@refChebi-IDmetaboliteproduction
6952116136hydrogen sulfideno
6952135581indoleno

enzymes

@refvalueactivityec
69521catalase+1.11.1.6
69521cytochrome oxidase+1.9.3.1
69521urease+3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    69521C16:027.6
    69521C18:1 w7c / C18:1 w6c40.9
    69521C19:0 cyclo w8c11.4
  • type of FA analysis: whole cell analysis
  • incubation medium: T5
  • agar/liquid: agar
  • incubation temperature: 50
  • incubation time: 3
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: HPLC
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 69521
  • sample type: Hot spring soil sample
  • geographic location: Yunnan province
  • country: China
  • origin.country: CHN
  • continent: Asia
  • enrichment culture: T5
  • enrichment culture duration: 14 days
  • enrichment culture temperature: 50

Sequence information

16S sequences

  • @ref: 20215
  • description: Azospirillum thermophilum 16S ribosomal RNA gene, partial sequence
  • accession: MH265951
  • length: 1509
  • database: nuccore
  • NCBI tax ID: 2202148

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
69521Azospirillum thermophilum CFH 70021GCA_003130795completencbi2202148
69521genome sequenceCP029352ncbi2202148
69521genome sequenceCP029353ncbi2202148
69521genome sequenceCP029354ncbi2202148
69521genome sequenceCP029355ncbi2202148

GC content

  • @ref: 69521
  • GC-content: 69.3
  • method: genome sequence analysis

External links

@ref: 20215

culture collection no.: KCTC 62259, CFH 70021, CCTCC AB 2018121

literature

  • topic: Phylogeny
  • Pubmed-ID: 31651377
  • title: Azospirillum thermophilum sp. nov., isolated from a hot spring.
  • authors: Zhao ZL, Ming H, Ding CL, Ji WL, Cheng LJ, Niu MM, Zhang YM, Zhang LY, Meng XL, Nie GX
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003788
  • year: 2020
  • mesh: Azospirillum/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hot Springs/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/url
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
69521Zhuo-li Zhao, Hong Ming, Chen-Long Ding, Wei-Li Ji, Li-Jiao Cheng, Ming-ming Niu, Yan-min Zhang, Ling-Yu Zhang, Xiao-Lin Meng, Guo-Xing NieAzospirillum thermophilum sp. nov., isolated from a hot spring10.1099/ijsem.0.003788