Strain identifier
BacDive ID: 1677
Type strain:
Species: Methylorosula polaris
Strain Designation: V-022
Strain history: <- L. V. Vasilyeva, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; V-022
NCBI tax ID(s): 519061 (species)
General
@ref: 16093
BacDive-ID: 1677
DSM-Number: 22001
keywords: 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped
description: Methylorosula polaris V-022 is an aerobe, Gram-negative, motile bacterium that was isolated from acidic tundra wetland soil.
NCBI tax id
- NCBI tax id: 519061
- Matching level: species
strain history
- @ref: 16093
- history: <- L. V. Vasilyeva, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; V-022
doi: 10.13145/bacdive1677.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Beijerinckiaceae
- genus: Methylorosula
- species: Methylorosula polaris
- full scientific name: Methylorosula polaris Berestovskaya et al. 2012
@ref: 16093
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Beijerinckiaceae
genus: Methylorosula
species: Methylorosula polaris
full scientific name: Methylorosula polaris Berestovskaya et al. 2012
strain designation: V-022
type strain: yes
Morphology
cell morphology
- @ref: 29133
- gram stain: negative
- cell length: 1.3-4.5 µm
- cell width: 0.5-0.6 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 29133
- production: yes
Culture and growth conditions
culture medium
- @ref: 16093
- name: METHYLOROSULA MEDIUM V-022 (DSMZ Medium 1403)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1403
- composition: Name: METHYLOROSULA MEDIUM V-022 (DSMZ Medium 1403) Composition: KH2PO4 0.07 g/l (NH4)2SO4 0.05 g/l Na2-EDTA 0.0005 g/l FeSO4 x 7 H2O 0.0002 g/l H3BO3 3e-05 g/l CoCl2 x 6 H2O 2e-05 g/l ZnSO4 x 7 H2O 1e-05 g/l MgCl2 x 6 H2O 3e-06 g/l Na2MoO4 x 2 H2O 3e-06 g/l NiCl2 x 6 H2O 2e-06 g/l CaCl2 x 2 H2O 1e-06 g/l Methanol Distilled water
culture temp
@ref | growth | type | temperature |
---|---|---|---|
16093 | positive | growth | 20-25 |
29133 | positive | growth | 4-30 |
29133 | positive | optimum | 20-25 |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
29133 | positive | growth | 4.0-7.8 | acidophile |
29133 | positive | optimum | 5.5-6 |
Physiology and metabolism
oxygen tolerance
- @ref: 29133
- oxygen tolerance: aerobe
halophily
- @ref: 29133
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: <2 %
Isolation, sampling and environmental information
isolation
- @ref: 16093
- sample type: acidic tundra wetland soil
- geographic location: Vorkuta
- country: Russia
- origin.country: RUS
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Soil |
#Environmental | #Terrestrial | #Wetland (Swamp) |
#Condition | #Acidic | |
#Climate | #Cold | #Tundra |
taxonmaps
- @ref: 69479
- File name: preview.99_161475.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_841;97_78649;98_106255;99_161475&stattab=map
- Last taxonomy: Methylorosula polaris subclade
- 16S sequence: EU586035
- Sequence Identity:
- Total samples: 224
- soil counts: 154
- aquatic counts: 10
- animal counts: 54
- plant counts: 6
Safety information
risk assessment
- @ref: 16093
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16093
- description: Methylorosula polaris strain V-022 16S ribosomal RNA gene, partial sequence
- accession: EU586035
- length: 1413
- database: ena
- NCBI tax ID: 519061
GC content
- @ref: 16093
- GC-content: 65.2
External links
@ref: 16093
culture collection no.: DSM 22001, VKM B-2485
straininfo link
- @ref: 71324
- straininfo: 403167
literature
- topic: Phylogeny
- Pubmed-ID: 21551336
- title: Methylorosula polaris gen. nov., sp. nov., an aerobic, facultatively methylotrophic psychrotolerant bacterium from tundra wetland soil.
- authors: Berestovskaya JJ, Kotsyurbenko OR, Tourova TP, Kolganova TV, Doronina NV, Golyshin PN, Vasilyeva LV
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.007005-0
- year: 2011
- mesh: Aerobiosis, Base Composition, Beijerinckiaceae/*classification/genetics/*isolation & purification/physiology, Carbon/*metabolism, Cluster Analysis, Cold Temperature, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Energy Metabolism, Hydrogen-Ion Concentration, Locomotion, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, *Soil Microbiology, Wetlands
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
16093 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22001) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-22001 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
29133 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25558 | 28776041 | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
71324 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID403167.1 | StrainInfo: A central database for resolving microbial strain identifiers |