Strain identifier

BacDive ID: 1677

Type strain: Yes

Species: Methylorosula polaris

Strain Designation: V-022

Strain history: <- L. V. Vasilyeva, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; V-022

NCBI tax ID(s): 519061 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 16093

BacDive-ID: 1677

DSM-Number: 22001

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped

description: Methylorosula polaris V-022 is an aerobe, Gram-negative, motile bacterium that was isolated from acidic tundra wetland soil.

NCBI tax id

  • NCBI tax id: 519061
  • Matching level: species

strain history

  • @ref: 16093
  • history: <- L. V. Vasilyeva, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; V-022

doi: 10.13145/bacdive1677.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Beijerinckiaceae
  • genus: Methylorosula
  • species: Methylorosula polaris
  • full scientific name: Methylorosula polaris Berestovskaya et al. 2012

@ref: 16093

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Beijerinckiaceae

genus: Methylorosula

species: Methylorosula polaris

full scientific name: Methylorosula polaris Berestovskaya et al. 2012

strain designation: V-022

type strain: yes

Morphology

cell morphology

  • @ref: 29133
  • gram stain: negative
  • cell length: 1.3-4.5 µm
  • cell width: 0.5-0.6 µm
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 29133
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 16093
  • name: METHYLOROSULA MEDIUM V-022 (DSMZ Medium 1403)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1403
  • composition: Name: METHYLOROSULA MEDIUM V-022 (DSMZ Medium 1403) Composition: KH2PO4 0.07 g/l (NH4)2SO4 0.05 g/l Na2-EDTA 0.0005 g/l FeSO4 x 7 H2O 0.0002 g/l H3BO3 3e-05 g/l CoCl2 x 6 H2O 2e-05 g/l ZnSO4 x 7 H2O 1e-05 g/l MgCl2 x 6 H2O 3e-06 g/l Na2MoO4 x 2 H2O 3e-06 g/l NiCl2 x 6 H2O 2e-06 g/l CaCl2 x 2 H2O 1e-06 g/l Methanol Distilled water

culture temp

@refgrowthtypetemperature
16093positivegrowth20-25
29133positivegrowth4-30
29133positiveoptimum20-25

culture pH

@refabilitytypepHPH range
29133positivegrowth4.0-7.8acidophile
29133positiveoptimum5.5-6

Physiology and metabolism

oxygen tolerance

  • @ref: 29133
  • oxygen tolerance: aerobe

halophily

  • @ref: 29133
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <2 %

Isolation, sampling and environmental information

isolation

  • @ref: 16093
  • sample type: acidic tundra wetland soil
  • geographic location: Vorkuta
  • country: Russia
  • origin.country: RUS
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Environmental#Terrestrial#Wetland (Swamp)
#Condition#Acidic
#Climate#Cold#Tundra

taxonmaps

  • @ref: 69479
  • File name: preview.99_161475.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_841;97_78649;98_106255;99_161475&stattab=map
  • Last taxonomy: Methylorosula polaris subclade
  • 16S sequence: EU586035
  • Sequence Identity:
  • Total samples: 224
  • soil counts: 154
  • aquatic counts: 10
  • animal counts: 54
  • plant counts: 6

Safety information

risk assessment

  • @ref: 16093
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16093
  • description: Methylorosula polaris strain V-022 16S ribosomal RNA gene, partial sequence
  • accession: EU586035
  • length: 1413
  • database: ena
  • NCBI tax ID: 519061

GC content

  • @ref: 16093
  • GC-content: 65.2

External links

@ref: 16093

culture collection no.: DSM 22001, VKM B-2485

straininfo link

  • @ref: 71324
  • straininfo: 403167

literature

  • topic: Phylogeny
  • Pubmed-ID: 21551336
  • title: Methylorosula polaris gen. nov., sp. nov., an aerobic, facultatively methylotrophic psychrotolerant bacterium from tundra wetland soil.
  • authors: Berestovskaya JJ, Kotsyurbenko OR, Tourova TP, Kolganova TV, Doronina NV, Golyshin PN, Vasilyeva LV
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.007005-0
  • year: 2011
  • mesh: Aerobiosis, Base Composition, Beijerinckiaceae/*classification/genetics/*isolation & purification/physiology, Carbon/*metabolism, Cluster Analysis, Cold Temperature, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Energy Metabolism, Hydrogen-Ion Concentration, Locomotion, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, *Soil Microbiology, Wetlands
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16093Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22001)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22001
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29133Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2555828776041
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71324Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID403167.1StrainInfo: A central database for resolving microbial strain identifiers