Strain identifier

BacDive ID: 16741

Type strain: Yes

Species: Laceyella sacchari

Strain Designation: A 978

Strain history: <- BCRC <- JCM <- KCC A-0214 <- CUB 618 <- J Lacey, A978

NCBI tax ID(s): 37482 (species)

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General

@ref: 10976

BacDive-ID: 16741

DSM-Number: 43356

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, pigmented, human pathogen

description: Laceyella sacchari A 978 is an aerobe, spore-forming, thermophilic human pathogen that has a Yellow-brown pigmentation and was isolated from bagasse.

NCBI tax id

  • NCBI tax id: 37482
  • Matching level: species

strain history

@refhistory
10976<- T. Cross, CUB <- J. Lacey, A 978
67770KCC A-0137 <-- J. Lacey A978.
67771<- BCRC <- JCM <- KCC A-0214 <- CUB 618 <- J Lacey, A978

doi: 10.13145/bacdive16741.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Thermoactinomycetaceae
  • genus: Laceyella
  • species: Laceyella sacchari
  • full scientific name: Laceyella sacchari (Lacey 1971) Yoon et al. 2005

  • synonyms

    @refsynonym
    20215Thermoactinomyces sacchari
    20215Thermoactinomyces thalpophilus

@ref: 10976

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Thermoactinomycetaceae

genus: Laceyella

species: Laceyella sacchari

full scientific name: Laceyella sacchari (Lacey 1971) Yoon et al. 2005

strain designation: A 978

type strain: yes

Morphology

cell morphology

@refgram stain
22925positive
67771positive

pigmentation

  • @ref: 22924
  • production: yes
  • name: Yellow-brown

multimedia

  • @ref: 10976
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43356.jpg
  • caption: Medium 554 50°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10976N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://mediadive.dsmz.de/medium/554Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
10976GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
22925CYCyes
10976CZAPEK PEPTONE AGAR (DSMZ Medium 83)yeshttps://mediadive.dsmz.de/medium/83Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
10976positivegrowth50thermophilic
22923positivegrowth55thermophilic
22923nogrowth28mesophilic
22924positivemaximum65thermophilic
22924positiveminimum35mesophilic
22925positiveminimum30mesophilic
22925positivegrowth55thermophilic
45050positivegrowth50thermophilic
67770positivegrowth50thermophilic
67771positivegrowth50thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
45050aerobe
67771aerobe

spore formation

  • @ref: 22925
  • spore description: Endospores are produced on sporophores up to 3 µm long
  • type of spore: endospore
  • spore formation: yes

compound production

@refcompound
22925Melanin
22926Hydrogen sulfide

halophily

@refsaltgrowthtested relationconcentration
22923NaClnogrowth2 %(w/v)
22925NaClnogrowth5 %(w/v)
22925NaClinconsistentgrowth1 %(w/v)

observation

@refobservation
67770quinones: MK-9, MK-8
67771quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2292317634D-glucose+builds acid from
2292316024D-mannose+builds acid from
2292363150D-rhamnose-builds acid from
2292316991dna+hydrolysis
2292317754glycerol+builds acid from
2292317368hypoxanthine+hydrolysis
2292317306maltose+builds acid from
2292318186tyrosine+hydrolysis
2292316199urea+hydrolysis
2292415824D-fructose+growth
2292416899D-mannitol+growth
229245291gelatin-hydrolysis
2292415428glycine+growth
2292417368hypoxanthine-hydrolysis
2292418019L-lysine-growth
2292417203L-proline-growth
2292462345L-rhamnose-growth
2292417716lactose+growth
2292428017starch+hydrolysis
2292427082trehalose+growth
2292516708adenine-hydrolysis
2292518305arbutin+/-hydrolysis
22925casein+hydrolysis
2292562968cellulose-growth
2292562968cellulose-hydrolysis
2292517029chitin+/-hydrolysis
2292515824D-fructose+growth
2292517634D-glucose+growth
2292516899D-mannitol+growth
2292516024D-mannose+/-growth
2292516634raffinose-growth
2292565327D-xylose-growth
2292516991dna+hydrolysis
229254767elastin+hydrolysis
229254853esculin+/-hydrolysis
229255291gelatin+hydrolysis
2292516235guanine-hydrolysis
2292517368hypoxanthine-hydrolysis
2292530849L-arabinose+/-growth
2292562345L-rhamnose-growth
2292517268myo-inositol-growth
2292533697rna+hydrolysis
2292528017starch+hydrolysis
2292517992sucrose+/-growth
2292553424tween 20+hydrolysis
2292553423tween 40+hydrolysis
2292553425tween 60+hydrolysis
2292553426tween 80+hydrolysis
2292518186tyrosine+/-hydrolysis
2292515318xanthine-hydrolysis
2292622599arabinose+/-growth
229265291gelatin-hydrolysis
2292628757fructose+growth
2292617234glucose+growth
2292615428glycine-growth
2292617203L-proline-growth
2292662345L-rhamnose-growth
2292617115L-serine-growth
2292616857L-threonine-growth
2292617716lactose+growth
2292617306maltose+growth
2292629864mannitol+growth
2292616634raffinose+/-growth
2292627082trehalose+growth
2292618186tyrosine+hydrolysis
2292617632nitrate-reduction

metabolite production

@refChebi-IDmetaboliteproduction
2292589634melaninyes
2292616136hydrogen sulfideyes

enzymes

@refvalueactivityec
22923urease+3.5.1.5
22924gelatinase-
22926gelatinase-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
10976bagasse
22925sugar cane, self-heated sugar cane bagasse, filter press muds, sugar mills and soil
45050Sugar cane bagasseTrinidadTrinidad and TobagoTTOMiddle and South America
67770Sugar cane bagasseTrinidad, West IndiesTrinidad and TobagoTTONorth America
67771From sugar cane bagasseTrinidad, West IndiesTrinidad and TobagoTTOMiddle and South America

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Decomposing plant
#Engineered#Industrial#Industrial production
#Environmental#Terrestrial#Mud (Sludge)
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)

taxonmaps

  • @ref: 69479
  • File name: preview.99_11727.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_284;96_5917;97_7121;98_8795;99_11727&stattab=map
  • Last taxonomy: Laceyella
  • 16S sequence: AJ251779
  • Sequence Identity:
  • Total samples: 13670
  • soil counts: 7909
  • aquatic counts: 1320
  • animal counts: 2143
  • plant counts: 2298

Safety information

risk assessment

  • @ref: 10976
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Thermoactinomyces sacchari partial 16S rRNA geneAJ2517791377ena37482
20218Thermoactinomyces sacchari KCTC 9790 16S ribosomal RNA gene, complete sequenceAF1387371459ena37482

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Laceyella sacchari strain DSM 4335637482.3wgspatric37482
66792Laceyella sacchari DSM 433562784746774draftimg37482
66792Laceyella sacchari JCM 31372784132157draftimg37482
67770Laceyella sacchari DSM 43356GCA_004343255scaffoldncbi37482

GC content

@refGC-contentmethod
1097648high performance liquid chromatography (HPLC)
6777148.0high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes88.879yes
anaerobicno98.737yes
halophileno89.261no
spore-formingyes95.62yes
glucose-utilyes92.364yes
aerobicyes92.77yes
motileno80.201no
flagellatedno90.796no
thermophileyes87.593no
glucose-fermentno92.538no

External links

@ref: 10976

culture collection no.: DSM 43356, ATCC 27375, CBS 701.70, CUB 618, IFO 13920, KCTC 9790, NBRC 13920, NCIB 10486, NCTC 10721, CCUG 7967, CBS 7001.70, JCM 3137, BCRC 13341, CCM 3274, CECT 5332, IMET 9713, JCM 3214, KACC 20089, LMG 21518, LMG 21673, MTCC 955, NCIMB 10486, NRRL B-16981, PCM 2375, VKM Ac-1360, VTT E-011981, PCM 237

straininfo link

  • @ref: 85759
  • straininfo: 45798

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10843048Phylogenetic analysis of the genus Thermoactinomyces based on 16S rDNA sequences.Yoon JH, Park YHInt J Syst Evol Microbiol10.1099/00207713-50-3-10812000DNA, Bacterial/analysis/genetics, DNA, Ribosomal/analysis/genetics, Micromonosporaceae/*classification/genetics, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, DNAGenetics
Phylogeny11034503DNA-DNA relatedness among Thermoactinomyces species: Thermoactinomyces candidus as a synonym of Thermoactinomyces vulgaris and Thermoactinomyces thalpophilus as a synonym of Thermoactinomyces sacchari.Yoon JH, Shin YK, Park YHInt J Syst Evol Microbiol10.1099/00207713-50-5-19052000DNA, Bacterial/*genetics, Micromonosporaceae/*classification/*genetics, *Nucleic Acid Hybridization
Phylogeny19915116Laceyella tengchongensis sp. nov., a thermophile isolated from soil of a volcano.Zhang J, Tang SK, Zhang YQ, Yu LY, Klenk HP, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.011767-02009Bacteria/*classification/genetics/*isolation & purification/metabolism, China, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Hot Temperature, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Volcanic Eruptions/*analysisMetabolism
Phylogeny24048866Thermoactinomyces daqus sp. nov., a thermophilic bacterium isolated from high-temperature Daqu.Yao S, Liu Y, Zhang M, Zhang X, Li H, Zhao T, Xin C, Xu L, Zhang B, Cheng CInt J Syst Evol Microbiol10.1099/ijs.0.055509-02013Alcoholic Beverages/*microbiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Fermentation, Hot Temperature, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spores, Bacterial, Thermoactinomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryStress
Phylogeny31310191Thermoflavimicrobium daqui sp. nov., a thermophilic microbe isolated from Moutai-flavour Daqu.Li DN, Huang W, Qiu SYInt J Syst Evol Microbiol10.1099/ijsem.0.0035282019Alcoholic Beverages/*microbiology, Bacillales/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spores, Bacterial, Vitamin K 2/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10976Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43356)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43356
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22923Sarah N. Buss, Jocelyn A. Cole, George E. Hannett, Elizabeth J. Nazarian, Leah Nazarian, An Coorevits, Anita Van Landschoot, Paul De Vos, Peter Schumann, Kimberlee A. Musser, William J. Wolfgang10.1099/ijs.0.050914-0Hazenella coriacea gen. nov., sp. nov., isolated from clinical specimensIJSEM 63: 4087-4093 201323728377
22924Jing Zhang, Shu-Kun Tang, Yu-Qin Zhang, Li-Yan Yu, Hans-Peter Klenk, Wen-Jun Li10.1099/ijs.0.011767-0Laceyella tengchongensis sp. nov., a thermophile isolated from soil of a volcanoIJSEM 60: 2226-2230 201019915116
22925Jung-Hoon Yoon, In-Gi Kim, Yong-Kook Shin, Yong-Ha Park10.1099/ijs.0.63203-0Proposal of the genus Thermoactinomyces sensu stricto and three new genera, Laceyella, Thermoflavimicrobium and Seinonella, on the basis of phenotypic, phylogenetic and chemotaxonomic analysesIJSEM 55: 395-400 200515653907
22926Su Yao, Yang Liu, Mingjuan Zhang, Xin Zhang, Hong Li, Ting Zhao, Chunhui Xin, Ling Xu, Bolin Zhang, Chi Cheng10.1099/ijs.0.055509-0Thermoactinomyces daqus sp. nov., a thermophilic bacterium isolated from high-temperature DaquIJSEM 64: 206-210 201424048866
45050Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 7967)https://www.ccug.se/strain?id=7967
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
85759Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45798.1StrainInfo: A central database for resolving microbial strain identifiers