Strain identifier
BacDive ID: 16736
Type strain: 
Species: Thermithiobacillus tepidarius
Strain history: <- D. P. Kelly <- A. P. Wood, Univ. Warwick, Coventry, U.K. {1983}
NCBI tax ID(s): 1123368 (strain), 929 (species)
version 8.1 (current version)
General
@ref: 1309
BacDive-ID: 16736
DSM-Number: 3134
keywords: genome sequence, 16S sequence, Bacteria, thermophilic, motile
description: Thermithiobacillus tepidarius DSM 3134 is a thermophilic, motile bacterium that was isolated from thermal sulfur spring.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 929 | species |
| 1123368 | strain |
strain history
- @ref: 1309
- history: <- D. P. Kelly <- A. P. Wood, Univ. Warwick, Coventry, U.K. {1983}
doi: 10.13145/bacdive16736.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Acidithiobacillia
- order: Acidithiobacillales
- family: Thermithiobacillaceae
- genus: Thermithiobacillus
- species: Thermithiobacillus tepidarius
- full scientific name: Thermithiobacillus tepidarius (Wood and Kelly 1985) Kelly and Wood 2000
synonyms
- @ref: 20215
- synonym: Thiobacillus tepidarius
@ref: 1309
domain: Bacteria
phylum: Proteobacteria
class: Acidithiobacillia
order: Acidithiobacillales
family: Thermithiobacillaceae
genus: Thermithiobacillus
species: Thermithiobacillus tepidarius
full scientific name: Thermithiobacillus tepidarius (Wood and Kelly 1985) Kelly and Wood 2000
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 97.576 | |
| 69480 | 100 | negative |
Culture and growth conditions
culture medium
- @ref: 1309
- name: THERMITHIOBACILLUS MEDIUM (DSMZ Medium 333)
- growth: yes
- link: https://mediadive.dsmz.de/medium/333
- composition: Name: THERMITHIOBACILLUS MEDIUM (DSMZ Medium 333) Composition: Agar 10.0 g/l K2HPO4 4.0 g/l KH2PO4 4.0 g/l K2S4O6 3.0 g/l MgSO4 x 7 H2O 0.8 g/l Na2-EDTA 0.5 g/l NH4Cl 0.4 g/l NaOH 0.11 g/l ZnSO4 x 7 H2O 0.11 g/l CaCl2 x 2 H2O 0.0734 g/l FeSO4 x 7 H2O 0.05 g/l MnCl2 x 4 H2O 0.025 g/l CoCl2 x 6 H2O 0.005 g/l (NH4)6Mo7O24 x 4 H2O 0.005 g/l CuSO4 x 5 H2O 0.002 g/l Bromocresol purple Distilled water
culture temp
- @ref: 1309
- growth: positive
- type: growth
- temperature: 43
- range: thermophilic
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 100 |
Isolation, sampling and environmental information
isolation
- @ref: 1309
- sample type: thermal sulfur spring
- geographic location: Great Roman Bath at Bath
- country: United Kingdom
- origin.country: GBR
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Sulfuric |
taxonmaps
- @ref: 69479
- File name: preview.99_3972.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_163;96_2019;97_2431;98_3007;99_3972&stattab=map
- Last taxonomy: Thermithiobacillus tepidarius subclade
- 16S sequence: AJ459801
- Sequence Identity:
- Total samples: 708
- soil counts: 178
- aquatic counts: 388
- animal counts: 117
- plant counts: 25
Safety information
risk assessment
- @ref: 1309
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Thiobacillus tepidarius 16S rRNA sequence | M79424 | 708 | ena | 929 |
| 20218 | Thiobacillus tepidarius 16S rRNA sequence | M79425 | 311 | ena | 929 |
| 1309 | Thermithiobacillus tepidarius 16S rRNA gene, strain DSM 3134 | AJ459801 | 1475 | ena | 929 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermithiobacillus tepidarius DSM 3134 | GCA_000423825 | scaffold | ncbi | 1123368 |
| 66792 | Thermithiobacillus tepidarius DSM 3134 | 1123368.4 | wgs | patric | 1123368 |
| 66792 | Thermithiobacillus tepidarius DSM 3134 | 2523533554 | draft | img | 1123368 |
GC content
- @ref: 1309
- GC-content: 66.8
- method: sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | yes | 89.729 | no |
| flagellated | no | 56.834 | no |
| gram-positive | no | 98.542 | no |
| anaerobic | no | 96.983 | no |
| aerobic | no | 73.077 | no |
| halophile | no | 80.151 | no |
| spore-forming | no | 94.13 | no |
| thermophile | yes | 77.066 | yes |
| glucose-util | yes | 51.444 | no |
| glucose-ferment | no | 87.699 | no |
External links
@ref: 1309
culture collection no.: DSM 3134, ATCC 43215
straininfo link
- @ref: 85754
- straininfo: 93015
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 26873326 | Thermithiobacillus plumbiphilus sp. nov., a sulfur-oxidizing bacterium isolated from lead sulfide. | Watanabe T, Miura A, Shinohara A, Kojima H, Fukui M | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000972 | 2016 | Autotrophic Processes, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gammaproteobacteria/*classification/genetics/isolation & purification, *Lead, Oxidation-Reduction, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sulfides, Sulfur/*chemistry, Sulfur-Reducing Bacteria/*classification/genetics/isolation & purification | Enzymology |
| 27708749 | Permanent draft genome of Thermithiobaclillus tepidarius DSM 3134(T), a moderately thermophilic, obligately chemolithoautotrophic member of the Acidithiobacillia. | Boden R, Hutt LP, Huntemann M, Clum A, Pillay M, Palaniappan K, Varghese N, Mikhailova N, Stamatis D, Reddy T, Ngan CY, Daum C, Shapiro N, Markowitz V, Ivanova N, Woyke T, Kyrpides N | Stand Genomic Sci | 10.1186/s40793-016-0188-0 | 2016 | |||
| 27777647 | Erratum to: Permanent draft genome of Thermithiobacillus tepidarius DSM 3134(T), a moderately thermophilic, obligately chemolithoautotrophic member of the Acidithiobacillia. | Boden R, Hutt LP, Huntemann M, Clum A, Pillay M, Palaniappan K, Varghese N, Mikhailova N, Stamatis D, Reddy T, Ngan CY, Daum C, Shapiro N, Markowitz V, Ivanova N, Woyke T, Kyrpides N | Stand Genomic Sci | 10.1186/s40793-016-0197-z | 2016 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1309 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3134) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3134 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 85754 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID93015.1 | StrainInfo: A central database for resolving microbial strain identifiers |