Strain identifier
BacDive ID: 16720
Type strain: 
Species: Thermus thermophilus
Strain Designation: RQ-1
Strain history: <- M. da Costa <- M. A. Santos
NCBI tax ID(s): 274 (species)
version 8.1 (current version)
General
@ref: 3525
BacDive-ID: 16720
DSM-Number: 9247
keywords: Bacteria, hyperthermophilic
description: Thermus thermophilus RQ-1 is a hyperthermophilic bacterium that was isolated from hot spring, cliff.
NCBI tax id
- NCBI tax id: 274
- Matching level: species
strain history
- @ref: 3525
- history: <- M. da Costa <- M. A. Santos
doi: 10.13145/bacdive16720.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/deinococcota
- domain: Bacteria
- phylum: Deinococcota
- class: Deinococci
- order: Thermales
- family: Thermaceae
- genus: Thermus
- species: Thermus thermophilus
- full scientific name: Thermus thermophilus (ex Oshima and Imahori 1974) Manaia et al. 1995
synonyms
- @ref: 20215
- synonym: Thermus thermophilus
@ref: 3525
domain: Bacteria
phylum: Deinococcus-Thermus
class: Deinococci
order: Thermales
family: Thermaceae
genus: Thermus
species: Thermus thermophilus
full scientific name: Thermus thermophilus (ex Oshima and Imahori 1974) Manaia et al. 1995
strain designation: RQ-1
type strain: no
Culture and growth conditions
culture medium
- @ref: 3525
- name: THERMUS 162 MEDIUM (DSMZ Medium 878)
- growth: yes
- link: https://mediadive.dsmz.de/medium/878
- composition: Name: THERMUS 162 MEDIUM (DSMZ Medium 878) Composition: Agar 28.0 g/l Na2HPO4 x 12 H2O 4.3 g/l Yeast extract 1.0 g/l Tryptone 1.0 g/l KH2PO4 0.544 g/l MgCl2 x 6 H2O 0.2 g/l Nitrilotriacetic acid 0.1 g/l CaSO4 x 2 H2O 0.04 g/l Fe(III) citrate 0.00122472 g/l MnSO4 x H2O 0.00114 g/l ZnSO4 x 7 H2O 0.00025 g/l H3BO3 0.00025 g/l CoCl2 x 6 H2O 2.25e-05 g/l CuSO4 x 5 H2O 1.25e-05 g/l Na2MoO4 x 2 H2O 1.25e-05 g/l H2SO4 Distilled water
culture temp
- @ref: 3525
- growth: positive
- type: growth
- temperature: 80
- range: hyperthermophilic
Isolation, sampling and environmental information
isolation
- @ref: 3525
- sample type: hot spring, cliff
- geographic location: Ribeira Quente, S. Miguel
- country: Portugal
- origin.country: PRT
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Environmental | #Terrestrial | #Geologic |
| #Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 3525
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
External links
@ref: 3525
culture collection no.: DSM 9247
straininfo link
- @ref: 85739
- straininfo: 48601
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 14526004 | Osmotic adaptation of Thermus thermophilus RQ-1: lesson from a mutant deficient in synthesis of trehalose. | Silva Z, Alarico S, Nobre A, Horlacher R, Marugg J, Boos W, Mingote AI, da Costa MS | J Bacteriol | 10.1128/JB.185.20.5943-5952.2003 | 2003 | *Adaptation, Physiological, Amino Acid Sequence, Culture Media, *Gene Deletion, Gene Expression Regulation, Bacterial, Glucosyltransferases/genetics/metabolism, Heat-Shock Response, Molecular Sequence Data, Osmotic Pressure, Phosphoric Monoester Hydrolases/genetics/metabolism, Sequence Analysis, DNA, Thermus thermophilus/*enzymology/genetics/*growth & development/physiology, Trehalose/*metabolism | Cultivation |
| Enzymology | 15455210 | Trehalose biosynthesis in Thermus thermophilus RQ-1: biochemical properties of the trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase. | Silva Z, Alarico S, da Costa MS | Extremophiles | 10.1007/s00792-004-0421-4 | 2004 | Adenosine Diphosphate/chemistry, Catalysis, Cloning, Molecular, Cobalt/chemistry, Dose-Response Relationship, Drug, Electrophoresis, Polyacrylamide Gel, Escherichia coli/enzymology/metabolism, Glucose/chemistry, Glucosyltransferases/*chemistry, Guanosine Diphosphate/chemistry, Hot Temperature, Hydrogen-Ion Concentration, Kinetics, Magnesium/chemistry, Mutation, Phosphoric Monoester Hydrolases/*chemistry, Phosphorylation, Recombinant Proteins/chemistry, Sugar Phosphates/*chemistry, Temperature, Thermus thermophilus/*enzymology, Time Factors, Trehalose/*analogs & derivatives/*biosynthesis/chemistry, Uridine Diphosphate/chemistry, Uridine Diphosphate Glucose/chemistry | Stress |
| Biotechnology | 17361428 | High yield of mannosylglycerate production by upshock fermentation and bacterial milking of trehalose-deficient mutant Thermus thermophilus RQ-1. | Egorova K, Grudieva T, Morinez C, Kube J, Santos H, da Costa MS, Antranikian G | Appl Microbiol Biotechnol | 10.1007/s00253-007-0915-y | 2007 | Fermentation/*physiology, Glyceric Acids, Mannose/*analogs & derivatives/biosynthesis, Osmotic Pressure, Thermus thermophilus/*genetics/*metabolism, Trehalose/*deficiency | Metabolism |
| Metabolism | 17726574 | Mannosylglycerate is essential for osmotic adjustment in Thermus thermophilus strains HB27 and RQ-1. | Alarico S, Empadinhas N, Mingote A, Simoes C, Santos MS, da Costa MS | Extremophiles | 10.1007/s00792-007-0106-x | 2007 | Adaptation, Physiological, Amino Acids/metabolism, Bacterial Proteins/genetics/metabolism, Cloning, Molecular, Glyceric Acids/*metabolism, Mannose/*analogs & derivatives/metabolism, Mannosyltransferases/genetics/metabolism, Mutation, Sodium Chloride/metabolism, Thermus thermophilus/enzymology/genetics/growth & development/*metabolism, Trehalose/metabolism, *Water-Electrolyte Balance | Enzymology |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 3525 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9247) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-9247 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 85739 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48601.1 | StrainInfo: A central database for resolving microbial strain identifiers |