Strain identifier

BacDive ID: 16718

Type strain: No

Species: Thermus thermophilus

Strain Designation: AT-62

Strain history: <- T. Saiki, AT-62 (Thermus flavus)

NCBI tax ID(s): 1316935 (strain), 274 (species)

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General

@ref: 355

BacDive-ID: 16718

DSM-Number: 674

keywords: genome sequence, 16S sequence, Bacteria, thermophilic, Gram-negative

description: Thermus thermophilus AT-62 is a thermophilic, Gram-negative bacterium that was isolated from hot spring water.

NCBI tax id

NCBI tax idMatching level
1316935strain
274species

strain history

  • @ref: 355
  • history: <- T. Saiki, AT-62 (Thermus flavus)

doi: 10.13145/bacdive16718.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/deinococcota
  • domain: Bacteria
  • phylum: Deinococcota
  • class: Deinococci
  • order: Thermales
  • family: Thermaceae
  • genus: Thermus
  • species: Thermus thermophilus
  • full scientific name: Thermus thermophilus (ex Oshima and Imahori 1974) Manaia et al. 1995
  • synonyms

    • @ref: 20215
    • synonym: Thermus thermophilus

@ref: 355

domain: Bacteria

phylum: Deinococcus-Thermus

class: Deinococci

order: Thermales

family: Thermaceae

genus: Thermus

species: Thermus thermophilus

full scientific name: Thermus thermophilus (ex Oshima and Imahori 1974) Manaia et al. 1995

strain designation: AT-62

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.995

Culture and growth conditions

culture medium

  • @ref: 355
  • name: THERMUS 162 MEDIUM (DSMZ Medium 878)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/878
  • composition: Name: THERMUS 162 MEDIUM (DSMZ Medium 878) Composition: Agar 28.0 g/l Na2HPO4 x 12 H2O 4.3 g/l Yeast extract 1.0 g/l Tryptone 1.0 g/l KH2PO4 0.544 g/l MgCl2 x 6 H2O 0.2 g/l Nitrilotriacetic acid 0.1 g/l CaSO4 x 2 H2O 0.04 g/l Fe(III) citrate 0.00122472 g/l MnSO4 x H2O 0.00114 g/l ZnSO4 x 7 H2O 0.00025 g/l H3BO3 0.00025 g/l CoCl2 x 6 H2O 2.25e-05 g/l CuSO4 x 5 H2O 1.25e-05 g/l Na2MoO4 x 2 H2O 1.25e-05 g/l H2SO4 Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
355positivegrowth75thermophilic
69480thermophilic100

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.926

compound production

  • @ref: 355
  • compound: restriction endonuclease TflI (TaqI)

Isolation, sampling and environmental information

isolation

  • @ref: 355
  • sample type: hot spring water
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 355
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: T.flavus 16S ribosomal RNA, part
  • accession: X58341
  • length: 1149
  • database: ena
  • NCBI tax ID: 274

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thermus thermophilus ATCC 339231316935.3wgspatric1316935
66792Thermus thermophilus ATCC 339232554235155draftimg1316935
66792Thermus thermophilus ATCC 33923GCA_000401495contigncbi1316935

GC content

  • @ref: 355
  • GC-content: 70.1

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno88.901no
gram-positiveno96.267no
anaerobicno95.119no
halophileno85.341no
spore-formingno92.179no
glucose-utilyes85.724no
flagellatedno97.623no
thermophileyes100yes
aerobicyes79.225no
glucose-fermentno86.71no

External links

@ref: 355

culture collection no.: DSM 674, ATCC 33923

straininfo link

  • @ref: 85737
  • straininfo: 40765

literature

Pubmed-IDtitleauthorsjournalDOIyeartopicmeshtopic2
16347571Resistance of Thermus spp. to Potassium Tellurite.Chiong M, Barra R, Gonzalez E, Vasquez CAppl Environ Microbiol10.1128/aem.54.2.610-612.19881988
23887916Genome Sequence of Thermus thermophilus ATCC 33923, a Thermostable Trehalose-Producing Strain.Jiang L, Lin M, Li X, Cui H, Xu X, Li S, Huang HGenome Announc10.1128/genomeA.00493-132013Genetics
25757335Molecular mutagenesis at Tyr-101 of the amylomaltase transcribed from a gene isolated from soil DNA.Watanasatitarpa S, Rudeekulthamrong P, Krusong K, Srisimarat W, Zimmermann W, Pongsawasdi P, Kaulpiboon JPrikl Biokhim Mikrobiol10.7868/s05551099140303252014EnzymologyAmino Acid Sequence, Bacterial Proteins/chemistry/*genetics/isolation & purification/metabolism, Cloning, Molecular, Cyclization, Cyclodextrins/*biosynthesis, DNA, Bacterial/*genetics/metabolism, Escherichia coli/enzymology/genetics, Glycogen Debranching Enzyme System/chemistry/*genetics/isolation & purification/metabolism, Hydrogen-Ion Concentration, Molecular Sequence Data, *Mutagenesis, Site-Directed, Oligosaccharides/metabolism, Recombinant Proteins/chemistry/genetics/isolation & purification/metabolism, Sequence Homology, Amino Acid, Soil/chemistry, Substrate Specificity, Thermus thermophilus/enzymology/genetics, *Transcription, Genetic, Trisaccharides/metabolismMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
355Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 674)https://www.dsmz.de/collection/catalogue/details/culture/DSM-674
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
85737Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40765.1StrainInfo: A central database for resolving microbial strain identifiers