Strain identifier
BacDive ID: 16718
Type strain:
Species: Thermus thermophilus
Strain Designation: AT-62
Strain history: <- T. Saiki, AT-62 (Thermus flavus)
NCBI tax ID(s): 1316935 (strain), 274 (species)
General
@ref: 355
BacDive-ID: 16718
DSM-Number: 674
keywords: genome sequence, 16S sequence, Bacteria, thermophilic, Gram-negative
description: Thermus thermophilus AT-62 is a thermophilic, Gram-negative bacterium that was isolated from hot spring water.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1316935 | strain |
274 | species |
strain history
- @ref: 355
- history: <- T. Saiki, AT-62 (Thermus flavus)
doi: 10.13145/bacdive16718.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/deinococcota
- domain: Bacteria
- phylum: Deinococcota
- class: Deinococci
- order: Thermales
- family: Thermaceae
- genus: Thermus
- species: Thermus thermophilus
- full scientific name: Thermus thermophilus (ex Oshima and Imahori 1974) Manaia et al. 1995
synonyms
- @ref: 20215
- synonym: Thermus thermophilus
@ref: 355
domain: Bacteria
phylum: Deinococcus-Thermus
class: Deinococci
order: Thermales
family: Thermaceae
genus: Thermus
species: Thermus thermophilus
full scientific name: Thermus thermophilus (ex Oshima and Imahori 1974) Manaia et al. 1995
strain designation: AT-62
type strain: no
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.995
Culture and growth conditions
culture medium
- @ref: 355
- name: THERMUS 162 MEDIUM (DSMZ Medium 878)
- growth: yes
- link: https://mediadive.dsmz.de/medium/878
- composition: Name: THERMUS 162 MEDIUM (DSMZ Medium 878) Composition: Agar 28.0 g/l Na2HPO4 x 12 H2O 4.3 g/l Yeast extract 1.0 g/l Tryptone 1.0 g/l KH2PO4 0.544 g/l MgCl2 x 6 H2O 0.2 g/l Nitrilotriacetic acid 0.1 g/l CaSO4 x 2 H2O 0.04 g/l Fe(III) citrate 0.00122472 g/l MnSO4 x H2O 0.00114 g/l ZnSO4 x 7 H2O 0.00025 g/l H3BO3 0.00025 g/l CoCl2 x 6 H2O 2.25e-05 g/l CuSO4 x 5 H2O 1.25e-05 g/l Na2MoO4 x 2 H2O 1.25e-05 g/l H2SO4 Distilled water
culture temp
@ref | growth | type | temperature | range | confidence |
---|---|---|---|---|---|
355 | positive | growth | 75 | thermophilic | |
69480 | thermophilic | 100 |
Physiology and metabolism
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.926 |
compound production
- @ref: 355
- compound: restriction endonuclease TflI (TaqI)
Isolation, sampling and environmental information
isolation
- @ref: 355
- sample type: hot spring water
- country: Japan
- origin.country: JPN
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Thermal spring |
#Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 355
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20218
- description: T.flavus 16S ribosomal RNA, part
- accession: X58341
- length: 1149
- database: ena
- NCBI tax ID: 274
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Thermus thermophilus ATCC 33923 | 1316935.3 | wgs | patric | 1316935 |
66792 | Thermus thermophilus ATCC 33923 | 2554235155 | draft | img | 1316935 |
66792 | Thermus thermophilus ATCC 33923 | GCA_000401495 | contig | ncbi | 1316935 |
GC content
- @ref: 355
- GC-content: 70.1
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 88.901 | no |
gram-positive | no | 96.267 | no |
anaerobic | no | 95.119 | no |
halophile | no | 85.341 | no |
spore-forming | no | 92.179 | no |
glucose-util | yes | 85.724 | no |
flagellated | no | 97.623 | no |
thermophile | yes | 100 | yes |
aerobic | yes | 79.225 | no |
glucose-ferment | no | 86.71 | no |
External links
@ref: 355
culture collection no.: DSM 674, ATCC 33923
straininfo link
- @ref: 85737
- straininfo: 40765
literature
Pubmed-ID | title | authors | journal | DOI | year | topic | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
16347571 | Resistance of Thermus spp. to Potassium Tellurite. | Chiong M, Barra R, Gonzalez E, Vasquez C | Appl Environ Microbiol | 10.1128/aem.54.2.610-612.1988 | 1988 | |||
23887916 | Genome Sequence of Thermus thermophilus ATCC 33923, a Thermostable Trehalose-Producing Strain. | Jiang L, Lin M, Li X, Cui H, Xu X, Li S, Huang H | Genome Announc | 10.1128/genomeA.00493-13 | 2013 | Genetics | ||
25757335 | Molecular mutagenesis at Tyr-101 of the amylomaltase transcribed from a gene isolated from soil DNA. | Watanasatitarpa S, Rudeekulthamrong P, Krusong K, Srisimarat W, Zimmermann W, Pongsawasdi P, Kaulpiboon J | Prikl Biokhim Mikrobiol | 10.7868/s0555109914030325 | 2014 | Enzymology | Amino Acid Sequence, Bacterial Proteins/chemistry/*genetics/isolation & purification/metabolism, Cloning, Molecular, Cyclization, Cyclodextrins/*biosynthesis, DNA, Bacterial/*genetics/metabolism, Escherichia coli/enzymology/genetics, Glycogen Debranching Enzyme System/chemistry/*genetics/isolation & purification/metabolism, Hydrogen-Ion Concentration, Molecular Sequence Data, *Mutagenesis, Site-Directed, Oligosaccharides/metabolism, Recombinant Proteins/chemistry/genetics/isolation & purification/metabolism, Sequence Homology, Amino Acid, Soil/chemistry, Substrate Specificity, Thermus thermophilus/enzymology/genetics, *Transcription, Genetic, Trisaccharides/metabolism | Metabolism |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
355 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 674) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-674 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
85737 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID40765.1 | StrainInfo: A central database for resolving microbial strain identifiers |