General

@ref: 1630

BacDive-ID: 16715

DSM-Number: 4250

keywords: Bacteria, thermophilic

description: Thermus sp. IB-21 is a thermophilic bacterium that was isolated from submarine hot spring.

NCBI tax id

• NCBI tax id: 275
• Matching level: species

strain history

• @ref: 1630
• history: <- G.A. Alfredsson, Univ. of Iceland, IB-21

doi: 10.13145/bacdive16715.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/deinococcota
• domain: Bacteria
• phylum: Deinococcota
• class: Deinococci
• order: Thermales
• family: Thermaceae
• genus: Thermus
• species: Thermus sp.
• full scientific name: Thermus Brock and Freeze 1969 (Approved Lists 1980)

@ref: 1630

domain: Bacteria

phylum: Deinococcus-Thermus

class: Deinococci

order: Thermales

family: Thermaceae

genus: Thermus

species: Thermus sp.

full scientific name: Thermus sp.

strain designation: IB-21

type strain: no

Culture and growth conditions

culture medium

• @ref: 1630
• name: MODIFIED THERMUS 162 MEDIUM (DSMZ Medium 630)
• growth: yes
• link: https://mediadive.dsmz.de/medium/630
• composition: Name: MODIFIED THERMUS 162 MEDIUM (DSMZ Medium 630) Composition: Agar 28.0 g/l Na2HPO4 x 12 H2O 4.3 g/l Yeast extract 2.5 g/l Tryptone 2.5 g/l KH2PO4 0.544 g/l MgCl2 x 6 H2O 0.2 g/l CaSO4 x 2 H2O 0.04 g/l Nitrilotriacetic acid 0.0064 g/l Fe(III) citrate 0.00122472 g/l FeCl2 x 4 H2O 0.0005 g/l MnCl2 x 4 H2O 0.00025 g/l CoCl2 x 4 H2O 0.00015 g/l CuCl2 x 2 H2O 2.5e-05 g/l Na2MoO4 x 2 H2O 2.5e-05 g/l H3BO3 1e-05 g/l NiCl2 x 6 H2O 1e-05 g/l Distilled water

culture temp

• @ref: 1630
• growth: positive
• type: growth
• temperature: 65
• range: thermophilic

Isolation, sampling and environmental information

isolation

• @ref: 1630
• sample type: submarine hot spring
• country: Iceland
• origin.country: ISL
• continent: Europe

isolation source categories

External links

@ref: 1630

culture collection no.: DSM 4250

straininfo link

• @ref: 85734
• straininfo: 47817

literature

• topic: Enzymology
• Pubmed-ID: 15748760
• title: Three forms of thermostable lactose-hydrolase from Thermus sp. IB-21: cloning, expression, and enzyme characterization.
• authors: Kang SK, Cho KK, Ahn JK, Bok JD, Kang SH, Woo JH, Lee HG, You SK, Choi YJ
• journal: J Biotechnol
• DOI: 10.1016/j.jbiotec.2004.07.019
• year: 2005
• mesh: Amino Acid Sequence, Cloning, Molecular/methods, Enzyme Activation, Enzyme Stability, Gene Expression Regulation, Bacterial/physiology, Glycoside Hydrolases/*chemistry/genetics/isolation & purification/*metabolism, Hydrogen-Ion Concentration, Isoenzymes/analysis/biosynthesis/chemistry/genetics, Kinetics, Lactose/*chemistry/*metabolism, Molecular Sequence Data, Protein Engineering/*methods, Recombinant Proteins/biosynthesis/chemistry/isolation & purification, Temperature, beta-Galactosidase/chemistry/genetics/isolation & purification/metabolism, beta-Glucosidase/chemistry/genetics/isolation & purification/metabolism
• topic2: Phylogeny

Reference