Archive BacDiveID:10.13145/bacdive16714.20230509.8.1

Strain identifier

BacDive ID: 16714

Type strain:

Species: Thermus aquaticus

Strain Designation: YT-1

Strain history: DSM 625 <-- T. D. Brock YT-1.

NCBI tax ID(s): 271 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 328

BacDive-ID: 16714

DSM-Number: 625

keywords: genome sequence, 16S sequence, Bacteria, thermophilic, Gram-negative

description: Thermus aquaticus YT-1 is a thermophilic, Gram-negative bacterium that was isolated from hot spring.

NCBI tax id

NCBI tax id: 271
Matching level: species

strain history

@ref	history
328	<- T. D. Brock; YT-1 {1975}
67770	DSM 625 <-- T. D. Brock YT-1.

doi: 10.13145/bacdive16714.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/deinococcota
domain: Bacteria
phylum: Deinococcota
class: Deinococci
order: Thermales
family: Thermaceae
genus: Thermus
species: Thermus aquaticus
full scientific name: Thermus aquaticus Brock and Freeze 1969 (Approved Lists 1980)

@ref: 328

domain: Bacteria

phylum: Deinococcus-Thermus

class: Deinococci

order: Thermales

family: Thermaceae

genus: Thermus

species: Thermus aquaticus

full scientific name: Thermus aquaticus Brock and Freeze 1969

strain designation: YT-1

type strain: yes

Morphology

cell morphology

@ref: 69480
gram stain: negative
confidence: 99.963

Culture and growth conditions

culture medium

@ref: 328
name: CASTENHOLZ MEDIUM (DSMZ Medium 86)
growth: yes
link: https://mediadive.dsmz.de/medium/86
composition: Name: CASTENHOLZ MEDIUM (DSMZ Medium 86) Composition: Yeast extract 1.0 g/l Tryptone 1.0 g/l NaNO3 0.689 g/l Na2HPO4 x 2 H2O 0.14 g/l KNO3 0.103 g/l MgSO4 x 7 H2O 0.1 g/l Nitrilotriacetic acid 0.1 g/l CaSO4 x 2 H2O 0.06 g/l NaCl 0.008 g/l MnSO4 x H2O 0.0022 g/l H3BO3 0.0005 g/l ZnSO4 x 7 H2O 0.0005 g/l FeCl3 x 6 H2O 0.00047 g/l CoCl2 x 6 H2O 4.6e-05 g/l CuSO4 x 5 H2O 2.5e-05 g/l Na2MoO4 x 2 H2O 2.5e-05 g/l Distilled water

culture temp

@ref	growth	type	temperature	range	confidence
328	positive	growth	70	thermophilic
67770	positive	growth	70	thermophilic
69480				thermophilic	99.999

Physiology and metabolism

spore formation

@ref	spore formation	confidence
69481	no	100
69480	no	99.708

compound production

@ref	compound
328	restriction endonuclease TflI (TaqI)
20216	Endonuclease Taq I
20216	Endonuclease II

observation

@ref: 67770
observation: quinones: MK-8

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
328	hot spring	Wyoming, Yellowstone Natl. Park	USA	USA	North America
67770	Mushroom Spring	Yellowstone National Park, WY	USA	USA	North America

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)

taxonmaps

@ref: 69479
File name: preview.99_3986.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_87;96_2025;97_2440;98_3018;99_3986&stattab=map
Last taxonomy: Thermus aquaticus subclade
16S sequence: L09663
Sequence Identity:
Total samples: 100
soil counts: 14
aquatic counts: 74
animal counts: 12

Safety information

risk assessment

@ref: 328
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 328
description: Thermus aquaticus strain YT-1 16S ribosomal RNA gene, partial sequence
accession: L09663
length: 1470
database: ena
NCBI tax ID: 271

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Thermus aquaticus strain YT-1	271.14	wgs	patric	271
66792	Thermus aquaticus YT-1	2648501156	draft	img	271
67770	Thermus aquaticus YT-1	GCA_001280255	scaffold	ncbi	271

GC content

@ref	GC-content	method
328	67.4
67770	67.4	Buoyant density centrifugation (BD)

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	no	100	no
motile	no	88.454	no
gram-positive	no	95.115	no
anaerobic	no	94.453	no
halophile	no	85.463	no
spore-forming	no	91.645	no
glucose-util	yes	88.156	no
aerobic	yes	78.382	no
flagellated	no	97.534	no
thermophile	yes	100	no
glucose-ferment	no	86.286	no

External links

@ref: 328

culture collection no.: DSM 625, ATCC 25104, JCM 10724, LMG 8924, NBRC 103206, NCIMB 11243

straininfo link

@ref: 85733
straininfo: 5027

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Metabolism	497233	Regulatory characteristics of phosphoenolpyruvate carboxylase from the extreme thermophile, Thermus aquaticus.	Sundaram TK, Bridger GP	Biochim Biophys Acta	10.1016/0005-2744(79)90160-8	1979	Acetyl Coenzyme A/metabolism, Allosteric Regulation, Carboxy-Lyases/metabolism, Kinetics, Phosphoenolpyruvate Carboxylase/antagonists & inhibitors/metabolism, Thermus/*enzymology	Enzymology
Phylogeny	678024	A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus.	Degryse E, Glansdorff N, Pierard A	Arch Microbiol	10.1007/BF00402307	1978	Aerobiosis, Belgium, Thermus/classification/cytology/physiology, Water Microbiology, *Water Pollution
Metabolism	730757	An isochizomer of TaqI from Thermus thermophilus HB8.	Sato S, Shinomiya T	J Biochem	10.1093/oxfordjournals.jbchem.a132252	1978	Binding Sites, Coliphages, DNA, Viral/metabolism, Endonucleases/isolation & purification/metabolism, Thermus/enzymology	Enzymology
Enzymology	1368580	Molecular cloning and nucleotide sequence of the aminopeptidase T gene of Thermus aquaticus YT-1 and its high-level expression in Escherichia coli.	Motoshima H, Azuma N, Kaminogawa S, Ono M, Minagawa E, Matsuzawa H, Ohta T, Yamauchi K	Agric Biol Chem		1990	Amino Acid Sequence, Aminopeptidases/chemistry/genetics, Bacterial Proteins, Base Sequence, Cloning, Molecular, Codon, Deoxyribonuclease HindIII, Enzyme Stability, Escherichia coli/genetics, Gene Expression, Hot Temperature, Molecular Sequence Data, Molecular Weight, Oligonucleotide Probes, Plasmids, Promoter Regions, Genetic/genetics, Restriction Mapping, Sequence Homology, Nucleic Acid, Thermus/enzymology/genetics	Genetics
Enzymology	1368764	Involvement of NH2-terminal pro-sequence in the production of active aqualysin I (a thermophilic serine protease) in Escherichia coli.	Lee YC, Miyata Y, Terada I, Ohta T, Matsuzawa H	Agric Biol Chem		1991	Amino Acid Sequence, Amino Acids/chemistry, Chromosome Deletion, Cloning, Molecular, Escherichia coli/genetics, Molecular Sequence Data, Mutagenesis, Site-Directed, Plasmids, Protein Precursors/metabolism, Serine Endopeptidases/biosynthesis/genetics/metabolism, Thermus/*enzymology	Genetics
Enzymology	1369078	Purification and characterization of a thermostable carboxypeptidase (carboxypeptidase Taq) from Thermus aquaticus YT-1.	Lee SH, Minagawa E, Taguchi H, Matsuzawa H, Ohta T, Kaminogawa S, Yamauchi K	Biosci Biotechnol Biochem	10.1271/bbb.56.1839	1992	Amino Acid Sequence, Biotechnology, Carboxypeptidases/chemistry/isolation & purification/metabolism, Enzyme Stability, Hydrogen-Ion Concentration, Molecular Sequence Data, Molecular Weight, Oligopeptides/chemistry, Substrate Specificity, Temperature, Thermus/enzymology	Biotechnology
Enzymology	1939005	Molecular cloning and nucleotide sequence of 3-isopropylmalate dehydrogenase gene (leuB) from an extreme thermophile, Thermus aquaticus YT-1.	Kirino H, Oshima T	J Biochem	10.1093/oxfordjournals.jbchem.a123470	1991	3-Isopropylmalate Dehydrogenase, Alcohol Oxidoreductases/chemistry/genetics, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Escherichia coli/enzymology, Hot Temperature, Molecular Sequence Data, Nucleic Acid Hybridization, Thermus/enzymology	Stress
Enzymology	2108962	Enhancement of the thermostability of subtilisin E by introduction of a disulfide bond engineered on the basis of structural comparison with a thermophilic serine protease.	Takagi H, Takahashi T, Momose H, Inouye M, Maeda Y, Matsuzawa H, Ohta T	J Biol Chem	S0021-9258(19)39230-0	1990	Amino Acid Sequence, Bacillus subtilis/enzymology, Base Sequence, Calorimetry, Differential Scanning, Disulfides, Enzyme Stability, Models, Molecular, Molecular Sequence Data, Mutation, Oligonucleotide Probes, Protein Conformation, Protein Denaturation, Sequence Homology, Nucleic Acid, Serine Endopeptidases/genetics/metabolism, Subtilisins/genetics/metabolism, Thermodynamics, Thermus/enzymology	Genetics
Enzymology	2182621	Unique precursor structure of an extracellular protease, aqualysin I, with NH2- and COOH-terminal pro-sequences and its processing in Escherichia coli.	Terada I, Kwon ST, Miyata Y, Matsuzawa H, Ohta T	J Biol Chem	S0021-9258(19)39186-0	1990	Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Bacterial/genetics, Enzyme Precursors/genetics, Escherichia coli/genetics, Gene Expression, Genes, Bacterial, Kinetics, Molecular Sequence Data, Molecular Weight, Oligonucleotide Probes, Plasmids, Protein Conformation, Restriction Mapping, Serine Endopeptidases/genetics/metabolism, Thermus/enzymology/*genetics	Genetics
Enzymology	2185236	Nucleotide sequence and characteristics of the gene for L-lactate dehydrogenase of Thermus aquaticus YT-1 and the deduced amino acid sequence of the enzyme.	Ono M, Matsuzawa H, Ohta T	J Biochem	10.1093/oxfordjournals.jbchem.a123004	1990	Amino Acid Sequence, Base Sequence, Cloning, Molecular, Codon, DNA, Bacterial/genetics, Escherichia coli/genetics, Genes, Bacterial, L-Lactate Dehydrogenase/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Recombinant Proteins, Restriction Mapping, Sequence Homology, Nucleic Acid, Thermus/enzymology/*genetics	Genetics
Enzymology	3142792	Crystallization and preliminary X-ray diffraction studies of intact EF-Tu from Thermus aquaticus YT-1.	Lippmann C, Betzel C, Dauter Z, Wilson K, Erdmann VA	FEBS Lett	10.1016/0014-5793(88)80355-7	1988	Crystallography, GTP-Binding Proteins, Molecular Weight, Peptide Elongation Factor Tu/isolation & purification, Protein Conformation, Thermus/enzymology, X-Ray Diffraction	Phylogeny
Enzymology	3162211	Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1.	Matsuzawa H, Tokugawa K, Hamaoki M, Mizoguchi M, Taguchi H, Terada I, Kwon ST, Ohta T	Eur J Biochem	10.1111/j.1432-1033.1988.tb13809.x	1988	Alkalies, Amino Acid Sequence, Amino Acids/analysis, Catalysis, Hydrogen-Ion Concentration, Hydrolysis, Molecular Weight, Pancreatic Elastase/isolation & purification, Serine Endopeptidases/isolation & purification, Temperature, Thermus/enzymology	Phylogeny
Enzymology	3240997	Determination of the positions of the disulfide bonds in aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT-1.	Kwon ST, Matsuzawa H, Ohta T	J Biochem	10.1093/oxfordjournals.jbchem.a122509	1988	Chromatography, High Pressure Liquid, Disulfides/analysis, Peptide Mapping, Serine Endopeptidases/analysis, Thermus/*enzymology
Enzymology	3286255	Nucleotide sequence of the gene for aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT-1 and characteristics of the deduced primary structure of the enzyme.	Kwon ST, Terada I, Matsuzawa H, Ohta T	Eur J Biochem	10.1111/j.1432-1033.1988.tb14025.x	1988	Amino Acid Sequence, Base Sequence, Cloning, Molecular, Codon, DNA/analysis, Escherichia coli/genetics, Genes, Molecular Sequence Data, Nucleic Acid Hybridization, Serine Endopeptidases/genetics, Thermus/enzymology/genetics	Genetics
Enzymology	3383846	NADH oxidase from the extreme thermophile Thermus aquaticus YT-1. Purification and characterisation.	Cocco D, Rinaldi A, Savini I, Cooper JM, Bannister JV	Eur J Biochem	10.1111/j.1432-1033.1988.tb14093.x	1988	Amino Acids/analysis, Chemical Phenomena, Chemistry, Electrophoresis, Polyacrylamide Gel, Flavins/analysis, Hydrogen Peroxide/metabolism, Hydrogen-Ion Concentration, Multienzyme Complexes/isolation & purification, NADH, NADPH Oxidoreductases/isolation & purification, Oxidation-Reduction, Peroxidases/isolation & purification, Thermus/enzymology	Phylogeny
Phylogeny	3806053	Isolation and preliminary taxonomic studies of Thermus strains isolated from Yellowstone National Park, USA.	Munster MJ, Munster AP, Woodrow JR, Sharp RJ	J Gen Microbiol	10.1099/00221287-132-6-1677	1986	Base Composition, DNA, Bacterial/classification, Thermus/*classification, Water Pollution/analysis, Wyoming
Enzymology	4019440	Fructose 1,6-bisphosphate-dependent L-lactate dehydrogenase from Thermus aquaticus YT-1, an extreme thermophile: activation by citrate and modification reagents and comparison with Thermus caldophilus GK24 L-lactate dehydrogenase.	Machida M, Matsuzawa H, Ohta T	J Biochem	10.1093/oxfordjournals.jbchem.a135132	1985	Amino Acids/analysis, Carboxylic Acids/pharmacology, Chemical Phenomena, Chemistry, Citrates/pharmacology, Enzyme Activation/drug effects, Fructosediphosphates/pharmacology, Hexosediphosphates/pharmacology, Hydrogen-Ion Concentration, Isoelectric Point, L-Lactate Dehydrogenase/metabolism, Molecular Weight, Species Specificity, Thermus/*enzymology	Phylogeny
Enzymology	7150670	[Isolation and properties of DNA polymerase from the extreme thermophilic bacterium Thermus ruber].	Kaledin AS, Sliusarenko AG, Gorodetskii SI	Biokhimiia		1982	Cations, DNA-Directed DNA Polymerase/isolation & purification/metabolism, Hot Temperature, Kinetics, Molecular Weight, Templates, Genetic, Thermus/enzymology	Phylogeny
Enzymology	7378495	[Isolation and properties of DNA polymerase from extreme thermophylic bacteria Thermus aquaticus YT-1].	Kaledin AS, Sliusarenko AG, Gorodetskii SI	Biokhimiia		1980	DNA-Directed DNA Polymerase/isolation & purification/metabolism, Hot Temperature, Kinetics, Magnesium/pharmacology, Manganese/pharmacology, Molecular Weight, Structure-Activity Relationship, Templates, Genetic, Thermus/enzymology	Phylogeny
Enzymology	7549094	Expression of aqualysin I (a thermophilic protease) in soluble form in Escherichia coli under a bacteriophage T7 promoter.	Sakamoto S, Terada I, Iijima M, Ohta T, Matsuzawa H	Biosci Biotechnol Biochem	10.1271/bbb.59.1438	1995	Bacteriophage T7/genetics, Base Sequence, Cloning, Molecular, Escherichia coli/genetics, Isopropyl Thiogalactoside/pharmacology, Molecular Sequence Data, Plasmids, Promoter Regions, Genetic, Recombinant Proteins/genetics, Serine Endopeptidases/drug effects/genetics/metabolism, Solubility, Thermus/enzymology	Genetics
Enzymology	7551039	A gene encoding a thermophilic alkaline serine proteinase from Thermus sp. strain Rt41A and its expression in Escherichia coli.	Munro GK, McHale RH, Saul DJ, Reeves RA, Bergquist PL	Microbiology (Reading)	10.1099/13500872-141-7-1731	1995	Amino Acid Sequence, Base Sequence, Chelating Agents/chemistry, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Enzyme Activation, Enzyme Stability, Escherichia coli/metabolism, Genes, Bacterial, Molecular Sequence Data, Phenylmethylsulfonyl Fluoride/chemistry, Polymerase Chain Reaction, Recombinant Fusion Proteins/biosynthesis, Serine Endopeptidases/biosynthesis/chemistry/genetics/isolation & purification/metabolism, Subtilisins/genetics, Temperature, Thermus/*genetics, Time Factors	Metabolism
Phylogeny	7710285	Polar lipids and fatty acid composition of Thermus strains from New Zealand.	Ferraz AS, Carreto L, Tenreiro S, Nobre MF, da Costa MS	Antonie Van Leeuwenhoek	10.1007/BF00882773	1994	Fatty Acids/analysis, Glycolipids/analysis, Lipids/analysis, New Zealand, Phospholipids/analysis, Phylogeny, Thermus/*chemistry/classification
Enzymology	7765282	Carboxypeptidase Taq, a thermostable zinc enzyme, from Thermus aquaticus YT-1: molecular cloning, sequencing, and expression of the encoding gene in Escherichia coli.	Lee SH, Taguchi H, Yoshimura E, Minagawa E, Kaminogawa S, Ohta T, Matsuzawa H	Biosci Biotechnol Biochem	10.1271/bbb.58.1490	1994	Amino Acid Sequence, Bacterial Proteins, Base Sequence, Binding Sites, Carboxypeptidases/chemistry/genetics/isolation & purification/metabolism, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Escherichia coli/genetics, Gene Expression/genetics, Molecular Sequence Data, Mutagenesis, Site-Directed, Nucleic Acid Hybridization, Plasmids, Thermus/enzymology/genetics, Zinc/analysis	Phylogeny
Enzymology	7765732	Fermentation conditions for efficient production of thermophilic protease in Escherichia coli harboring a plasmid.	Sakamoto S, Terada I, Iijima M, Matsuzawa H, Ohta T	Appl Microbiol Biotechnol	10.1007/BF00173922	1994	Biotechnology, Cloning, Molecular, Culture Media, Enzyme Induction/drug effects, Escherichia coli/drug effects/enzymology/genetics, Fermentation, Glucose/metabolism, Isopropyl Thiogalactoside/pharmacology, Nitrogen/metabolism, Plasmids/genetics, Serine Endopeptidases/biosynthesis/*genetics, Temperature, Thermus/enzymology/genetics	Biotechnology
Enzymology	7896728	Overproduction of Thermus aquaticus DNA polymerase and its structural analysis by ion-spray mass spectrometry.	Ishino Y, Ueno T, Miyagi M, Uemori T, Imamura M, Tsunasawa S, Kato I	J Biochem	10.1093/oxfordjournals.jbchem.a124622	1994	Amino Acid Sequence, Base Sequence, Chromatography, High Pressure Liquid, Cloning, Molecular, DNA-Directed DNA Polymerase/chemistry/genetics, Enzyme Stability, Gene Expression, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Analysis, Spectrometry, Mass, Secondary Ion, Thermus/*enzymology	Genetics
Enzymology	8113181	The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization.	Angov E, Camerini-Otero RD	J Bacteriol	10.1128/jb.176.5.1405-1412.1994	1994	Amino Acid Sequence, Base Sequence, Cloning, Molecular, Codon/genetics, DNA Primers, DNA, Single-Stranded/metabolism, Escherichia coli/metabolism, Gene Expression, Genes, Bacterial, Hot Temperature, Kinetics, Molecular Sequence Data, Rec A Recombinases/biosynthesis/genetics/isolation & purification, Recombinant Proteins/biosynthesis/isolation & purification/metabolism, Restriction Mapping, Sequence Homology, Amino Acid, Thermodynamics, Thermus/*genetics/metabolism	Metabolism
Metabolism	8617781	Identification and characterization of a thermostable MutS homolog from Thermus aquaticus.	Biswas I, Hsieh P	J Biol Chem	10.1074/jbc.271.9.5040	1996	Adenosine Triphosphatases/biosynthesis/chemistry/metabolism, Amino Acid Sequence, Bacterial Proteins/biosynthesis/chemistry/metabolism, Base Sequence, Cloning, Molecular, Conserved Sequence, DNA/chemistry/metabolism, DNA Primers, DNA Repair, DNA-Binding Proteins, Enzyme Stability, Escherichia coli, Escherichia coli Proteins, Gene Expression, Genes, Bacterial, Hot Temperature, Molecular Sequence Data, MutS DNA Mismatch-Binding Protein, Polymerase Chain Reaction, Recombinant Proteins/biosynthesis/isolation & purification/metabolism, Sequence Homology, Amino Acid, Substrate Specificity, Thermus/genetics/metabolism	Enzymology
Enzymology	8679935	Cloning and overexpression of thermostable DNA polymerase gene in Escherichia coli.	Jin C, Liu H, Yang S, Zhang S	Chin J Biotechnol		1995	Base Sequence, Cloning, Molecular, DNA, Bacterial/genetics, DNA-Directed DNA Polymerase/genetics, Electrophoresis, Agar Gel, Electrophoresis, Polyacrylamide Gel, Escherichia coli/genetics, Gene Expression Regulation, Bacterial/genetics, Molecular Sequence Data, Plasmids/genetics, Polymerase Chain Reaction, RNA-Directed DNA Polymerase/genetics, Taq Polymerase, Temperature	Genetics
Enzymology	9163743	Cloning and analysis of the DNA polymerase-encoding gene from Thermus caldophilus GK24.	Kwon ST, Kim JS, Park JH, Kim HK, Lee DS	Mol Cells		1997	Amino Acid Sequence, Amino Acids/analysis, Base Sequence, Cloning, Molecular, Codon/genetics, DNA Primers/genetics, DNA, Bacterial/genetics, DNA-Directed DNA Polymerase/chemistry/genetics, Escherichia coli/enzymology/genetics, Gene Expression, Genes, Bacterial, Genetic Vectors, Molecular Sequence Data, Plasmids, Polymerase Chain Reaction, Restriction Mapping, Sequence Homology, Amino Acid, Thermus/enzymology/*genetics	Genetics
Enzymology	9362117	Cloning of genes of the aminopeptidase T family from Thermus thermophilus HB8 and Bacillus stearothermophilus NCIB8924: apparent similarity to the leucyl aminopeptidase family.	Motoshima H, Minagawa E, Tsukasaki F, Kaminogawa S	Biosci Biotechnol Biochem	10.1271/bbb.61.1710	1997	Amino Acid Sequence, Aminopeptidases/genetics, Bacterial Proteins, Base Sequence, Cloning, Molecular, Culture Media, Escherichia coli/genetics, Fluorometry, Gene Expression Regulation, Enzymologic, Geobacillus stearothermophilus/enzymology/genetics, Leucyl Aminopeptidase/genetics, Molecular Sequence Data, Molecular Weight, Plasmids, Sequence Homology, Amino Acid, Substrate Specificity, Thermus thermophilus/*enzymology/genetics	Cultivation
Metabolism	9418238	Role of the COOH-terminal pro-sequence of aqualysin I (a heat-stable serine protease) in its extracellular secretion by Thermus thermophilus.	Kim DW, Lee YC, Matsuzawa H	FEMS Microbiol Lett	10.1111/j.1574-6968.1997.tb12750.x	1997	Amino Acid Sequence, Bacterial Proteins/genetics/metabolism, Culture Media/chemistry, Gene Deletion, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Immunoblotting, Molecular Sequence Data, Plasmids, Protein Structure, Tertiary, Serine Endopeptidases/chemistry/genetics/metabolism, Thermus thermophilus/enzymology/genetics/metabolism	Genetics
Enzymology	9490070	Purification and characterisation of NADH oxidase from Thermus aquaticus YT-1 and evidence that it functions in a peroxide-reduction system.	Toomey D, Mayhew SG	Eur J Biochem	10.1046/j.1432-1327.1998.2510935.x	1998	Amino Acid Sequence, Chromatography, Affinity, Chromatography, DEAE-Cellulose, Enzyme Stability, Flavin-Adenine Dinucleotide/analysis, Kinetics, Macromolecular Substances, Molecular Sequence Data, Molecular Weight, Multienzyme Complexes/chemistry/isolation & purification/metabolism, NADH, NADPH Oxidoreductases/chemistry/isolation & purification/metabolism, Oxidation-Reduction, Photochemistry, Sequence Alignment, Sequence Homology, Amino Acid, Spectrophotometry, Thermodynamics, Thermus/*enzymology/growth & development	Genetics
Enzymology	9882104	P1 specificity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1-substituted derivatives of Streptomyces subtilisin inhibitor.	Tanaka T, Matsuzawa H, Kojima S, Kumagai I, Miura K, Ohta T	Biosci Biotechnol Biochem	10.1271/bbb.62.2035	1998	Bacterial Proteins/pharmacology, Binding Sites, Kinetics, Oligopeptides/metabolism, Serine Endopeptidases/metabolism, Serine Proteinase Inhibitors/pharmacology, Substrate Specificity, Thermus/enzymology	Metabolism
Enzymology	9918492	Recombinant His-tagged DNA polymerase. II. Cloning and purification of Thermus aquaticus recombinant DNA polymerase (Stoffel fragment).	Dabrowski S, Kur J	Acta Biochim Pol		1998	Amino Acid Sequence, Base Sequence, Chromatography, Affinity, Cloning, Molecular, DNA, Bacterial, Electrophoresis, Polyacrylamide Gel, Genetic Vectors, Histidine/chemistry, Molecular Sequence Data, Recombinant Proteins/chemistry/genetics/isolation & purification, Taq Polymerase/chemistry/genetics/isolation & purification, Thermus/*enzymology	Genetics
Enzymology	10076001	Identification, cloning and expression of p25, an AT-rich DNA-binding protein from the extreme thermophile, Thermus aquaticus YT-1.	Du X, Pene JJ	Nucleic Acids Res	10.1093/nar/27.7.1690	1999	Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Bacterial/metabolism, DNA-Binding Proteins/genetics/isolation & purification/metabolism, Electrophoresis, Polyacrylamide Gel, Escherichia coli/genetics, Molecular Sequence Data, Protein Binding, Sequence Homology, Amino Acid, Temperature, Thermus/genetics	Metabolism
Enzymology	10089430	Crystallization and preliminary crystallographic analysis of an NADH oxidase that functions in peroxide reduction in Thermus aquaticus YT-1.	Mac Sweeney A, D'Arcy A, Higgins TM, Mayhew SG, Toomey D, Walsh MA	Acta Crystallogr D Biol Crystallogr	10.1107/S090744499800941X	1999	Crystallization, Crystallography, X-Ray, Enzyme Stability, Gram-Positive Bacteria/enzymology, Multienzyme Complexes/chemistry/isolation & purification/metabolism, NADH, NADPH Oxidoreductases/chemistry/isolation & purification/metabolism, Oxidation-Reduction, Peroxides/metabolism, Salmonella typhimurium/enzymology, Temperature, Thermus/*enzymology	Metabolism
Enzymology	10348901	Identification and designing of the S3 site of aqualysin I, a thermophilic subtilisin-related serine protease.	Tanaka T, Matsuzawa H, Ohta T	J Biochem	10.1093/oxfordjournals.jbchem.a022380	1999	Amino Acid Sequence, Base Sequence, Catalytic Domain/genetics, DNA Primers/genetics, Drug Design, Kinetics, Models, Molecular, Mutagenesis, Site-Directed, Oligopeptides, Protein Conformation, Serine Endopeptidases/*chemistry/genetics/metabolism, Substrate Specificity, Subtilisins/chemistry/genetics/metabolism, Thermus/enzymology/genetics	Metabolism
Enzymology	10446366	Weakly bound calcium ions involved in the thermostability of aqualysin I, a heat-stable subtilisin-type protease of Thermus aquaticus YT-1.	Lin SJ, Yoshimura E, Sakai H, Wakagi T, Matsuzawa H	Biochim Biophys Acta	10.1016/s0167-4838(99)00140-5	1999	Binding Sites, Calcium/chemistry/pharmacology, Calcium Chloride, Chelating Agents, Circular Dichroism, Edetic Acid, Enzyme Stability/drug effects, Hot Temperature, Metals, Protein Denaturation, Resins, Synthetic, Serine Endopeptidases/chemistry, Thermodynamics, Thermus/*enzymology	Stress
Metabolism	11027666	Requirement for the COOH-terminal pro-sequence in the translocation of aqualysin I across the cytoplasmic membrane in Escherichia coli.	Kim DW, Matsuzawa H	Biochem Biophys Res Commun	10.1006/bbrc.2000.3657	2000	Cell Membrane/metabolism, Enzyme Activation, Escherichia coli/cytology/metabolism, Mutation/genetics, Protein Conformation, Protein Precursors/chemistry/genetics/metabolism, Protein Processing, Post-Translational, Protein Sorting Signals/genetics/physiology, Protein Transport, Recombinant Proteins/chemistry/metabolism, Serine Endopeptidases/chemistry/genetics/metabolism, Thermus/*enzymology	Enzymology
Enzymology	11237691	Molecular cloning, expression, purification, and characterization of fructose-1,6-bisphosphate aldolase from Thermus aquaticus.	Sauve V, Sygusch J	Protein Expr Purif	10.1006/prep.2000.1380	2001	Amino Acid Sequence, Cloning, Molecular, Enzyme Stability, Escherichia coli, Fructose-Bisphosphate Aldolase/chemistry/genetics/isolation & purification/metabolism, Kinetics, Molecular Sequence Data, Protein Structure, Quaternary, Recombinant Proteins/chemistry/isolation & purification/metabolism, Scattering, Radiation, Sequence Alignment, Sequence Analysis, DNA, Temperature, Thermus/*enzymology/genetics	Phylogeny
Enzymology	12111745	Independently expressed N-terminal pro-domain of aqualysin I precursor complements the folding of its mature domain to active form in Escherichia coli.	Kim JY, Choi YL, Cho YS, Kim CH, Lee YC	J Basic Microbiol	10.1002/1521-4028(200206)42:3<181::AID-JOBM181>3.0.CO;2-8	2002	Amino Acid Sequence, Electrophoresis, Polyacrylamide Gel, Escherichia coli/enzymology/genetics, Genetic Complementation Test, Molecular Sequence Data, Mutagenesis, Site-Directed, Plasmids, Promoter Regions, Genetic, Protein Folding, Protein Precursors/chemistry/metabolism, Protein Sorting Signals, Serine Endopeptidases/chemistry/genetics/*metabolism, Structure-Activity Relationship	Genetics
Enzymology	12767813	High-level expression, secretion, and purification of the thermostable aqualysin I from Thermus aquaticus YT-1 in Pichia pastoris.	Oledzka G, Dabrowski S, Kur J	Protein Expr Purif	10.1016/s1046-5928(03)00060-3	2003	Amino Acid Sequence, DNA Primers/genetics, Enzyme Stability, Hot Temperature, Molecular Sequence Data, Pichia/enzymology/genetics, Plasmids/genetics, Recombinant Proteins/biosynthesis/isolation & purification/metabolism, Serine Endopeptidases/biosynthesis/isolation & purification/metabolism, Thermus/enzymology/genetics	Stress
Biotechnology	15195968	Quantification of intra- and extra-cellular thermophilic lipase/esterase production by Thermus sp.	Dominguez A, Sanroman A, Fucinos P, Rua ML, Pastrana L, Longo MA	Biotechnol Lett	10.1023/b:bile.0000024092.27943.75	2004	Biotechnology/methods, Cell Proliferation, Esterases/chemistry, Esters, Fatty Acids/chemistry, Hydrolysis, Lipase/chemistry, Substrate Specificity, Temperature, Thermus/*enzymology, Thermus thermophilus/enzymology, Time Factors	Enzymology
Enzymology	15299524	Purification, crystallization and preliminary X-ray investigation of aqualysin I, a heat-stable serine protease.	Green PR, Oliver JD, Strickland LC, Toerner DR, Matsuzawa H, Ohta T	Acta Crystallogr D Biol Crystallogr	10.1107/S0907444992012083	1993
Pathogenicity	16348594	Production and Extracellular Secretion of Aqualysin I (a Thermophilic Subtilisin-Type Protease) in a Host-Vector System for Thermus thermophilus.	Touhara N, Taguchi H, Koyama Y, Ohta T, Matsuzawa H	Appl Environ Microbiol	10.1128/aem.57.11.3385-3387.1991	1991		Biotechnology
Enzymology	17169970	Role of proline residues in conferring thermostability on aqualysin I.	Sakaguchi M, Matsuzaki M, Niimiya K, Seino J, Sugahara Y, Kawakita M	J Biochem	10.1093/jb/mvm025	2006	Amino Acid Sequence, Enzyme Stability, Kinetics, Molecular Sequence Data, Mutation, Proline/metabolism, Protein Conformation, Serine Endopeptidases/chemistry/genetics/metabolism, Temperature, Thermus/enzymology	Genetics
Enzymology	18216068	Role of disulphide bonds in a thermophilic serine protease aqualysin I from Thermus aquaticus YT-1.	Sakaguchi M, Takezawa M, Nakazawa R, Nozawa K, Kusakawa T, Nagasawa T, Sugahara Y, Kawakita M	J Biochem	10.1093/jb/mvn007	2008	Bacterial Proteins/chemistry/genetics, Caseins/metabolism, Cystine/chemistry, Enzyme Stability, Mutation, Serine Endopeptidases/chemistry/genetics, Temperature, Thermus/enzymology	Metabolism
Metabolism	18623530	Thermophilic biodegradation of BTEX by two Thermus Species.	Chen CI, Taylor RT	Biotechnol Bioeng	10.1002/bit.260480609	1995
Enzymology	18685213	Construction of an expression system for aqualysin I in Escherichia coli that gives a markedly improved yield of the enzyme protein.	Sakaguchi M, Niimiya K, Takezawa M, Toki T, Sugahara Y, Kawakita M	Biosci Biotechnol Biochem	10.1271/bbb.80132	2008	Escherichia coli/enzymology/genetics, Gene Expression/genetics, Plasmids/genetics, Recombinant Fusion Proteins/genetics/metabolism, Serine Endopeptidases/genetics/isolation & purification/*metabolism	Metabolism
Enzymology	19233950	Stimulation of expression of a silica-induced protein (Sip) in Thermus thermophilus by supersaturated silicic acid.	Doi K, Fujino Y, Inagaki F, Kawatsu R, Tahara M, Ohshima T, Okaue Y, Yokoyama T, Iwai S, Ogata S	Appl Environ Microbiol	10.1128/AEM.02387-08	2009	Amino Acid Sequence, Bacterial Proteins/biosynthesis/chemistry, DNA, Bacterial/chemistry/genetics, Electrophoresis, Gel, Two-Dimensional, Geologic Sediments/microbiology, Hot Springs, Japan, Molecular Sequence Data, Molecular Weight, Phylogeny, Proteome/analysis, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Silicic Acid/metabolism, Thermus thermophilus/drug effects/growth & development/isolation & purification/metabolism	Phylogeny
Phylogeny	19628590	Thermus islandicus sp. nov., a mixotrophic sulfur-oxidizing bacterium isolated from the Torfajokull geothermal area.	Bjornsdottir SH, Petursdottir SK, Hreggvidsson GO, Skirnisdottir S, Hjorleifsdottir S, Arnfinnsson J, Kristjansson JK	Int J Syst Evol Microbiol	10.1099/ijs.0.007013-0	2009	DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Iceland, Molecular Sequence Data, Oxidation-Reduction, Phospholipids/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Sulfur/metabolism, Thermus/classification/genetics/*isolation & purification/metabolism	Metabolism
Pathogenicity	21454596	A novel exopolysaccharide from the biofilm of Thermus aquaticus YT-1 induces the immune response through Toll-like receptor 2.	Lin MH, Yang YL, Chen YP, Hua KF, Lu CP, Sheu F, Lin GH, Tsay SS, Liang SM, Wu SH	J Biol Chem	10.1074/jbc.M110.200113	2011	Adjuvants, Immunologic/pharmacology, Animals, Biofilms, Carbohydrate Conformation, HEK293 Cells, Humans, Interleukin-6/genetics/immunology, Macrophage Activation/drug effects/immunology, Macrophages/immunology, Mice, Mice, Knockout, Myeloid Differentiation Factor 88/genetics/immunology, Polysaccharides, Bacterial/immunology/pharmacology, Thermus/physiology, Toll-Like Receptor 2/genetics/immunology, Tumor Necrosis Factor-alpha/genetics/immunology
Enzymology	25136511	Highly conserved salt bridge stabilizes a proteinase K subfamily enzyme, Aqualysin I, from Thermus aquaticus YT-1.	Sakaguchi M, Osaku K, Maejima S, Ohno N, Sugahara Y, Oyama F, Kawakita M	AMB Express	10.1186/s13568-014-0059-2	2014
Enzymology	27392689	Stability of Thermostable Enzyme, Aqualysin I ; a Subtilisin-type Serine Protease from Thermus aquaticus YT-1.	Tanaka T, Matsuzawa H, Ohta T	Biosci Biotechnol Biochem	10.1271/bbb.62.1806	1998
	27393070	Substrate Specificity of Aqualysin I Altered by an Organic Solvent, DMSO.	Tanaka T, Matsuzawa H, Ohta T	Biosci Biotechnol Biochem	10.1271/bbb.63.446	1999
	27393587	Substrate Specificity of Aqualysin I, a Bacterial Thermophilic Alkaline Serine Protease from Thermus aquaticus YT-1: Comparison with Proteinase K, Subtilisin BPN' and Subtilisin Carlsberg.	Tanaka T, Matsuzawa H, Ohta T	Biosci Biotechnol Biochem	10.1271/bbb.62.2161	1998
Metabolism	28911108	The third restriction-modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities.	Skowron PM, Anton BP, Czajkowska E, Zebrowska J, Sulecka E, Krefft D, Jezewska-Frackowiak J, Zolnierkiewicz O, Witkowska M, Morgan RD, Wilson GG, Fomenkov A, Roberts RJ, Zylicz-Stachula A	Nucleic Acids Res	10.1093/nar/gkx599	2017	Amino Acid Sequence, Bacterial Proteins/genetics/metabolism, Cloning, Molecular, DNA Cleavage, Deoxyribonucleases, Type II Site-Specific/genetics/metabolism, Escherichia coli/genetics/metabolism, Gene Expression, Isoenzymes/genetics/metabolism, Molecular Weight, Nucleotide Motifs, Plasmids/chemistry/metabolism, Recombinant Proteins/genetics/metabolism, Sequence Alignment, Sequence Homology, Amino Acid, Substrate Specificity, Thermus/*enzymology/genetics	Enzymology

Reference

@id	authors	catalogue	doi/url	title
328	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 625)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-625
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216	Curators of the HKI		http://www.leibniz-hki.de/de/	Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481	Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch		10.21203/rs.3.rs-2527258/v1	Predictions based on the model GenomeNet Sporulation v. 1
85733	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID5027.1	StrainInfo: A central database for resolving microbial strain identifiers