Strain identifier
BacDive ID: 16694
Type strain:
Species: Thermosyntropha lipolytica
Strain Designation: JW/VS-265
Strain history: <- J. Wiegel, University of Georgia, Department of Microbiology, Athens, USA; JW/VS-265 <- V. A. Svetlichnyi {1995}
NCBI tax ID(s): 1123382 (strain), 54294 (species)
General
@ref: 4157
BacDive-ID: 16694
DSM-Number: 11003
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-positive
description: Thermosyntropha lipolytica JW/VS-265 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from sediment.
NCBI tax id
NCBI tax id | Matching level |
---|---|
54294 | species |
1123382 | strain |
strain history
- @ref: 4157
- history: <- J. Wiegel, University of Georgia, Department of Microbiology, Athens, USA; JW/VS-265 <- V. A. Svetlichnyi {1995}
doi: 10.13145/bacdive16694.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Syntrophomonadaceae
- genus: Thermosyntropha
- species: Thermosyntropha lipolytica
- full scientific name: Thermosyntropha lipolytica Svetlitshnyi et al. 1996
@ref: 4157
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Syntrophomonadaceae
genus: Thermosyntropha
species: Thermosyntropha lipolytica
full scientific name: Thermosyntropha lipolytica Svetlitshnyi et al. 1996
strain designation: JW/VS-265
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 99.225
Culture and growth conditions
culture medium
- @ref: 4157
- name: THERMOSYNTROPHA MEDIUM (DSMZ Medium 731)
- growth: yes
- link: https://mediadive.dsmz.de/medium/731
- composition: Name: THERMOSYNTROPHA MEDIUM (DSMZ Medium 731) Composition: Yeast extract 9.8912 g/l NaHCO3 2.96736 g/l Na2CO3 2.96736 g/l NH4Cl 0.98912 g/l Na2S x 9 H2O 0.49456 g/l K2HPO4 0.296736 g/l KCl 0.296736 g/l MgCl2 x 6 H2O 0.296736 g/l L-Cysteine HCl x H2O 0.148368 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l ZnSO4 x 7 H2O 0.00178042 g/l CoSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Biotin 1.97824e-05 g/l Folic acid 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water
culture temp
@ref | growth | type | temperature | range | confidence |
---|---|---|---|---|---|
4157 | positive | growth | 60 | thermophilic | |
69480 | thermophilic | 99.503 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
4157 | anaerobe | |
69480 | anaerobe | 99.6 |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | yes | 100 |
69480 | yes | 95.001 |
compound production
- @ref: 4157
- compound: lipase
Isolation, sampling and environmental information
isolation
- @ref: 4157
- sample type: sediment
- geographic location: Lake Bogoria
- country: Kenya
- origin.country: KEN
- continent: Africa
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Sediment |
#Environmental | #Terrestrial | #Sediment |
taxonmaps
- @ref: 69479
- File name: preview.99_169550.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4449;96_63283;97_82075;98_111160;99_169550&stattab=map
- Last taxonomy: Thermosyntropha lipolytica subclade
- 16S sequence: NR_026356
- Sequence Identity:
- Total samples: 3
- aquatic counts: 3
Safety information
risk assessment
- @ref: 4157
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 4157
- description: Thermosyntropha lipolytica strain JW265 16S ribosomal RNA, partial sequence
- accession: NR_026356
- length: 1458
- database: nuccore
- NCBI tax ID: 54294
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Thermosyntropha lipolytica DSM 11003 | GCA_900129805 | contig | ncbi | 1123382 |
66792 | Thermosyntropha lipolytica DSM 11003 | 1123382.5 | wgs | patric | 1123382 |
66792 | Thermosyntropha lipolytica DSM 11003 | 2585428064 | draft | img | 1123382 |
GC content
- @ref: 4157
- GC-content: 43.6
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
motile | yes | 80.587 | no |
flagellated | no | 90.656 | no |
gram-positive | no | 86.196 | no |
anaerobic | yes | 98.652 | yes |
aerobic | no | 95.656 | no |
halophile | no | 88.364 | no |
spore-forming | yes | 70.242 | no |
glucose-util | yes | 69.285 | no |
thermophile | yes | 99.479 | no |
glucose-ferment | no | 86.112 | no |
External links
@ref: 4157
culture collection no.: DSM 11003, ATCC 700317
straininfo link
- @ref: 85713
- straininfo: 43876
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Metabolism | 8863447 | Thermosyntropha lipolytica gen. nov., sp. nov., a lipolytic, anaerobic, alkalitolerant, thermophilic bacterium utilizing short- and long-chain fatty acids in syntrophic coculture with a methanogenic archaeum. | Svetlitshnyi V, Rainey F, Wiegel J | Int J Syst Bacteriol | 10.1099/00207713-46-4-1131 | 1996 | Bacteria, Anaerobic/*metabolism, Base Composition, Base Sequence, Coculture Techniques, DNA, Ribosomal/chemistry, Fatty Acids/*metabolism, Fatty Acids, Volatile/metabolism, Hydrogen-Ion Concentration, Methanobacterium/*metabolism, Molecular Sequence Data, Temperature | Genetics |
Enzymology | 17933930 | Purification and characterization of two highly thermophilic alkaline lipases from Thermosyntropha lipolytica. | Salameh MA, Wiegel J | Appl Environ Microbiol | 10.1128/AEM.01509-07 | 2007 | Bacteria, Anaerobic/*enzymology, Bacterial Proteins/chemistry/isolation & purification/*metabolism, Catalysis/drug effects, Edetic Acid/pharmacology, Electrophoresis, Polyacrylamide Gel, Enzyme Activation/drug effects, Enzyme Stability, Hydrogen-Ion Concentration, Ions/pharmacology, Isoenzymes/chemistry/isolation & purification/metabolism, Lipase/chemistry/isolation & purification/*metabolism, Molecular Sequence Data, Molecular Weight, Substrate Specificity, Temperature | Phylogeny |
Enzymology | 19593631 | Synthesis of fatty acid esters and diacylglycerols at elevated temperatures by alkalithermophilic lipases from Thermosyntropha lipolytica. | Salameh MA, Wiegel J | J Ind Microbiol Biotechnol | 10.1007/s10295-009-0610-3 | 2009 | Diglycerides/*metabolism, Enzyme Stability, Fatty Acids/*metabolism, Gram-Positive Bacteria/*enzymology, *Hot Temperature, Lipase/*isolation & purification/*metabolism | Metabolism |
20361033 | Effects of Detergents on Activity, Thermostability and Aggregation of Two Alkalithermophilic Lipases from Thermosyntropha lipolytica. | Salameh MA, Wiegel J | Open Biochem J | 10.2174/1874091X01004010022 | 2010 | |||
Phylogeny | 21571939 | Thermosyntropha tengcongensis sp. nov., a thermophilic bacterium that degrades long-chain fatty acids syntrophically. | Zhang F, Liu X, Dong X | Int J Syst Evol Microbiol | 10.1099/ijs.0.033456-0 | 2011 | Bacteria, Anaerobic/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, Coculture Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Gram-Positive Bacteria/genetics/metabolism, Hot Springs/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Metabolism |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
4157 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11003) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11003 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
85713 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID43876.1 | StrainInfo: A central database for resolving microbial strain identifiers |