Strain identifier

BacDive ID: 16694

Type strain: Yes

Species: Thermosyntropha lipolytica

Strain Designation: JW/VS-265

Strain history: <- J. Wiegel, University of Georgia, Department of Microbiology, Athens, USA; JW/VS-265 <- V. A. Svetlichnyi {1995}

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4157

BacDive-ID: 16694

DSM-Number: 11003

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-positive

description: Thermosyntropha lipolytica JW/VS-265 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from sediment.

NCBI tax id

NCBI tax idMatching level
54294species
1123382strain

strain history

  • @ref: 4157
  • history: <- J. Wiegel, University of Georgia, Department of Microbiology, Athens, USA; JW/VS-265 <- V. A. Svetlichnyi {1995}

doi: 10.13145/bacdive16694.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Syntrophomonadaceae
  • genus: Thermosyntropha
  • species: Thermosyntropha lipolytica
  • full scientific name: Thermosyntropha lipolytica Svetlitshnyi et al. 1996

@ref: 4157

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Syntrophomonadaceae

genus: Thermosyntropha

species: Thermosyntropha lipolytica

full scientific name: Thermosyntropha lipolytica Svetlitshnyi et al. 1996

strain designation: JW/VS-265

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 99.225

Culture and growth conditions

culture medium

  • @ref: 4157
  • name: THERMOSYNTROPHA MEDIUM (DSMZ Medium 731)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/731
  • composition: Name: THERMOSYNTROPHA MEDIUM (DSMZ Medium 731) Composition: Yeast extract 9.8912 g/l NaHCO3 2.96736 g/l Na2CO3 2.96736 g/l NH4Cl 0.98912 g/l Na2S x 9 H2O 0.49456 g/l K2HPO4 0.296736 g/l KCl 0.296736 g/l MgCl2 x 6 H2O 0.296736 g/l L-Cysteine HCl x H2O 0.148368 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l ZnSO4 x 7 H2O 0.00178042 g/l CoSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Biotin 1.97824e-05 g/l Folic acid 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
4157positivegrowth60thermophilic
69480thermophilic99.503

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
4157anaerobe
69480anaerobe99.6

spore formation

@refspore formationconfidence
69481yes100
69480yes95.001

compound production

  • @ref: 4157
  • compound: lipase

Isolation, sampling and environmental information

isolation

  • @ref: 4157
  • sample type: sediment
  • geographic location: Lake Bogoria
  • country: Kenya
  • origin.country: KEN
  • continent: Africa

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_169550.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4449;96_63283;97_82075;98_111160;99_169550&stattab=map
  • Last taxonomy: Thermosyntropha lipolytica subclade
  • 16S sequence: NR_026356
  • Sequence Identity:
  • Total samples: 3
  • aquatic counts: 3

Safety information

risk assessment

  • @ref: 4157
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4157
  • description: Thermosyntropha lipolytica strain JW265 16S ribosomal RNA, partial sequence
  • accession: NR_026356
  • length: 1458
  • database: nuccore
  • NCBI tax ID: 54294

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thermosyntropha lipolytica DSM 11003GCA_900129805contigncbi1123382
66792Thermosyntropha lipolytica DSM 110031123382.5wgspatric1123382
66792Thermosyntropha lipolytica DSM 110032585428064draftimg1123382

GC content

  • @ref: 4157
  • GC-content: 43.6
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes80.587no
flagellatedno90.656no
gram-positiveno86.196no
anaerobicyes98.652yes
aerobicno95.656no
halophileno88.364no
spore-formingyes70.242no
glucose-utilyes69.285no
thermophileyes99.479no
glucose-fermentno86.112no

External links

@ref: 4157

culture collection no.: DSM 11003, ATCC 700317

straininfo link

  • @ref: 85713
  • straininfo: 43876

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism8863447Thermosyntropha lipolytica gen. nov., sp. nov., a lipolytic, anaerobic, alkalitolerant, thermophilic bacterium utilizing short- and long-chain fatty acids in syntrophic coculture with a methanogenic archaeum.Svetlitshnyi V, Rainey F, Wiegel JInt J Syst Bacteriol10.1099/00207713-46-4-11311996Bacteria, Anaerobic/*metabolism, Base Composition, Base Sequence, Coculture Techniques, DNA, Ribosomal/chemistry, Fatty Acids/*metabolism, Fatty Acids, Volatile/metabolism, Hydrogen-Ion Concentration, Methanobacterium/*metabolism, Molecular Sequence Data, TemperatureGenetics
Enzymology17933930Purification and characterization of two highly thermophilic alkaline lipases from Thermosyntropha lipolytica.Salameh MA, Wiegel JAppl Environ Microbiol10.1128/AEM.01509-072007Bacteria, Anaerobic/*enzymology, Bacterial Proteins/chemistry/isolation & purification/*metabolism, Catalysis/drug effects, Edetic Acid/pharmacology, Electrophoresis, Polyacrylamide Gel, Enzyme Activation/drug effects, Enzyme Stability, Hydrogen-Ion Concentration, Ions/pharmacology, Isoenzymes/chemistry/isolation & purification/metabolism, Lipase/chemistry/isolation & purification/*metabolism, Molecular Sequence Data, Molecular Weight, Substrate Specificity, TemperaturePhylogeny
Enzymology19593631Synthesis of fatty acid esters and diacylglycerols at elevated temperatures by alkalithermophilic lipases from Thermosyntropha lipolytica.Salameh MA, Wiegel JJ Ind Microbiol Biotechnol10.1007/s10295-009-0610-32009Diglycerides/*metabolism, Enzyme Stability, Fatty Acids/*metabolism, Gram-Positive Bacteria/*enzymology, *Hot Temperature, Lipase/*isolation & purification/*metabolismMetabolism
20361033Effects of Detergents on Activity, Thermostability and Aggregation of Two Alkalithermophilic Lipases from Thermosyntropha lipolytica.Salameh MA, Wiegel JOpen Biochem J10.2174/1874091X010040100222010
Phylogeny21571939Thermosyntropha tengcongensis sp. nov., a thermophilic bacterium that degrades long-chain fatty acids syntrophically.Zhang F, Liu X, Dong XInt J Syst Evol Microbiol10.1099/ijs.0.033456-02011Bacteria, Anaerobic/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, Coculture Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Gram-Positive Bacteria/genetics/metabolism, Hot Springs/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4157Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11003)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11003
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
85713Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID43876.1StrainInfo: A central database for resolving microbial strain identifiers