Strain identifier

BacDive ID: 16658

Type strain: Yes

Species: Syntrophus buswellii

Strain Designation: DM-2

Variant: Isotype of BacDive ID 16660 & 16662

Strain history: <- M.P. Bryant, DM-2

NCBI tax ID(s): 43774 (species)

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General

@ref: 1167

BacDive-ID: 16658

DSM-Number: 102352

keywords: Bacteria, anaerobe, mesophilic

description: Syntrophus buswellii DM-2 is an anaerobe, mesophilic bacterium that was isolated from muncipal primary anaerobic digester.

NCBI tax id

  • NCBI tax id: 43774
  • Matching level: species

strain history

@refhistory
20218<- M.P. Bryant, DM-2
1167<- M.P. Bryant, DM-2

doi: 10.13145/bacdive16658.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Syntrophobacterales
  • family: Syntrophaceae
  • genus: Syntrophus
  • species: Syntrophus buswellii
  • full scientific name: Syntrophus buswellii Mountfort et al. 1984

@ref: 1167

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Syntrophobacterales

family: Syntrophaceae

genus: Syntrophus

species: Syntrophus buswellii

full scientific name: Syntrophus buswellii Mountfort et al. 1984

strain designation: DM-2

variant: Isotype of BacDive ID 16660 & 16662

type strain: yes

Morphology

colony morphology

  • @ref: 1167
  • incubation period: 8-14 days

Culture and growth conditions

culture medium

  • @ref: 1167
  • name: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/212
  • composition: Name: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications) Composition: Na2SO4 2.79163 g/l Na-benzoate 1.99402 g/l Na2CO3 1.49551 g/l Trypticase peptone 0.997008 g/l KH2PO4 0.498505 g/l NaCl 0.398803 g/l NH4Cl 0.398803 g/l MgCl2 x 6 H2O 0.329013 g/l Na2S x 9 H2O 0.299103 g/l L-Cysteine HCl x H2O 0.299103 g/l CaCl2 x 2 H2O 0.0498505 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498505 g/l NaOH 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l Nicotinic acid 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Calcium pantothenate 9.97009e-05 g/l Vitamin B12 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Distilled water

culture temp

  • @ref: 1167
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1167
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 1167
  • sample type: muncipal primary anaerobic digester
  • geographic location: Illinois, Urbana
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Biodegradation
  • Cat3: #Anaerobic digestor

Safety information

risk assessment

  • @ref: 1167
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 1167

culture collection no.: DSM 102352, DSM 2612A

straininfo link

  • @ref: 85679
  • straininfo: 405491

literature

  • topic: Metabolism
  • Pubmed-ID: 8867639
  • title: Activation and degradation of benzoate, 3-phenylpropionate and crotonate by Syntrophus buswellii strain GA. Evidence for electron-transport phosphorylation during crotonate respiration.
  • authors: Auburger G, Winter J
  • journal: Appl Microbiol Biotechnol
  • DOI: 10.1007/BF00178623
  • year: 1996
  • mesh: Bacteria, Anaerobic/enzymology/*metabolism, Benzoates/*metabolism, Benzoic Acid, Biodegradation, Environmental, Cell Membrane/chemistry, Crotonates/*metabolism, Cytochrome b Group/analysis, Cytochrome c Group/analysis, Cytoplasm/chemistry, Desulfovibrio/metabolism, Electron Transport, Models, Chemical, Phenylpropionates/*metabolism, Phosphorylation
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1167Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102352)https://www.dsmz.de/collection/catalogue/details/culture/DSM-102352
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
85679Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID405491.1StrainInfo: A central database for resolving microbial strain identifiers