Strain identifier

BacDive ID: 16655

Type strain: Yes

Species: Sulfuracidifex metallicus

Strain history: DSM 6482 <-- K. O. Stetter and G. Huber Kra23.

NCBI tax ID(s): 47303 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2693

BacDive-ID: 16655

DSM-Number: 6482

keywords: genome sequence, 16S sequence, Archaea, thermophilic

description: Sulfuracidifex metallicus DSM 6482 is a thermophilic archaeon that was isolated from solfataric field.

NCBI tax id

  • NCBI tax id: 47303
  • Matching level: species

strain history

@refhistory
2693<- K. O. Stetter, Univ. Regensburg, Germany; Kra 23 <- G. Huber and K. O. Stetter {1985}
67770DSM 6482 <-- K. O. Stetter and G. Huber Kra23.

doi: 10.13145/bacdive16655.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/thermoproteota
  • domain: Archaea
  • phylum: Thermoproteota
  • class: Thermoprotei
  • order: Sulfolobales
  • family: Sulfolobaceae
  • genus: Sulfuracidifex
  • species: Sulfuracidifex metallicus
  • full scientific name: Sulfuracidifex metallicus (Huber and Stetter 1992) Itoh et al. 2020
  • synonyms

    • @ref: 20215
    • synonym: Sulfolobus metallicus

@ref: 2693

domain: Archaea

phylum: Crenarchaeota

class: Thermoprotei

order: Sulfolobales

family: Sulfolobaceae

genus: Sulfuracidifex

species: Sulfuracidifex metallicus

full scientific name: Sulfuracidifex metallicus (Huber and Stetter 1992) Itoh et al. 2020

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 2693
  • name: SULFOLOBUS MEDIUM (DSMZ Medium 88)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/88
  • composition: Name: SULFOLOBUS MEDIUM (DSMZ Medium 88; with strain-specific modifications) Composition: Sulfur powder 4.9505 g/l (NH4)2SO4 1.28713 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l Yeast extract 0.19802 g/l CaCl2 x 2 H2O 0.0693069 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
2693positivegrowth65thermophilic
67770positivegrowth65thermophilic

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
2693solfataric fieldKrafla areaIcelandISLEurope
67770Solfatara in KraflaIcelandISLEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Volcanic

Safety information

risk assessment

  • @ref: 2693
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Sulfolobus metallicus 16S rRNA gene, almost full-length sequenceD855191433ena523847
2693S.metallicus DNA for 16S ribosomal RNA geneX904791395ena523847

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sulfolobus metallicus DSM 6482, JCM 91842681813032draftimg523847
67770Sulfuracidifex metallicus DSM 6482 = JCM 9184GCA_001316045contigncbi523847
67770Sulfuracidifex metallicus DSM 6482 = JCM 9184GCA_009729515contigncbi523847
66792Sulfolobus metallicus DSM 6482 = JCM 9184523847.5wgspatric523847

GC content

@refGC-contentmethod
269338.0
6777037high performance liquid chromatography (HPLC)
6777038thermal denaturation, midpoint method (Tm)

External links

@ref: 2693

culture collection no.: DSM 6482, Kra 23, JCM 9184, NBRC 15436, IFO 15436

straininfo link

  • @ref: 85676
  • straininfo: 48214

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism25488256Use of lectins to in situ visualize glycoconjugates of extracellular polymeric substances in acidophilic archaeal biofilms.Zhang RY, Neu TR, Bellenberg S, Kuhlicke U, Sand W, Vera MMicrob Biotechnol10.1111/1751-7915.121882014Archaea/growth & development/*metabolism/*physiology, Biofilms/*growth & development, Biopolymers/*analysis, Glycoconjugates/*analysis, Iron/metabolism, Lectins/*metabolism, Microscopy, Fluorescence, Protein Binding, Sulfides/metabolism
Metabolism26169631Visualization and analysis of EPS glycoconjugates of the thermoacidophilic archaeon Sulfolobus metallicus.Zhang R, Neu TR, Zhang Y, Bellenberg S, Kuhlicke U, Li Q, Sand W, Vera MAppl Microbiol Biotechnol10.1007/s00253-015-6775-y2015Biofilms/growth & development, Biopolymers/*analysis, Carbohydrates/analysis, Glycoconjugates/*analysis, Lectins/metabolism, Microscopy, Confocal, Protein Binding, Proteins/analysis, Staining and Labeling, Sulfolobus/*chemistry/growth & development/physiology, Sulfur
Metabolism27388200Biofilm formation and interspecies interactions in mixed cultures of thermo-acidophilic archaea Acidianus spp. and Sulfolobus metallicus.Castro C, Zhang R, Liu J, Bellenberg S, Neu TR, Donati E, Sand W, Vera MRes Microbiol10.1016/j.resmic.2016.06.0052016Acidianus/*physiology, Bacterial Adhesion, Biofilms/*growth & development, *Environmental Microbiology, Iron/metabolism, *Microbial Interactions, Microscopy, Confocal, Sulfides/metabolism, Sulfolobus/*physiology, Sulfur/metabolism
Phylogeny31958046Sulfuracidifex tepidarius gen. nov., sp. nov. and transfer of Sulfolobus metallicus Huber and Stetter 1992 to the genus Sulfuracidifex as Sulfuracidifex metallicus comb. nov.Itoh T, Miura T, Sakai HD, Kato S, Ohkuma M, Takashina TInt J Syst Evol Microbiol10.1099/ijsem.0.0039812020Autotrophic Processes, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Japan, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Sulfolobaceae/*classification/isolation & purification, Sulfolobus/*classificationTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2693Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6482)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6482
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
85676Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48214.1StrainInfo: A central database for resolving microbial strain identifiers