Strain identifier

BacDive ID: 16653

Type strain: No

Species: Saccharolobus solfataricus

Strain Designation: MT-4

Strain history: <- A. Gambacorta <- A. Gambacorta and M. De Rosa, MT-4 ("Caldariella acidophila").

NCBI tax ID(s): 2287 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2333

BacDive-ID: 16653

DSM-Number: 5833

keywords: Archaea, hyperthermophilic

description: Saccharolobus solfataricus MT-4 is a hyperthermophilic archaeon that was isolated from solfatara.

NCBI tax id

  • NCBI tax id: 2287
  • Matching level: species

strain history

  • @ref: 2333
  • history: <- A. Gambacorta <- A. Gambacorta and M. De Rosa, MT-4 ("Caldariella acidophila").

doi: 10.13145/bacdive16653.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/thermoproteota
  • domain: Archaea
  • phylum: Thermoproteota
  • class: Thermoprotei
  • order: Sulfolobales
  • family: Sulfolobaceae
  • genus: Saccharolobus
  • species: Saccharolobus solfataricus
  • full scientific name: Saccharolobus solfataricus (Zillig et al. 1980) Sakai and Kurosawa 2018
  • synonyms

    • @ref: 20215
    • synonym: Sulfolobus solfataricus

@ref: 2333

domain: Archaea

phylum: Crenarchaeota

class: Thermoprotei

order: Sulfolobales

family: Sulfolobaceae

genus: Saccharolobus

species: Saccharolobus solfataricus

full scientific name: Saccharolobus solfataricus (Zillig et al. 1980) Sakai and Kurosawa 2018

strain designation: MT-4

type strain: no

Culture and growth conditions

culture medium

  • @ref: 2333
  • name: SACCHAROLOBUS (MT-4) MEDIUM (DSMZ Medium 182a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/182a
  • composition: Name: SACCHAROLOBUS (MT-4) MEDIUM (DSMZ Medium 182a) Composition: KH2PO4 3.1 g/l (NH4)2SO4 2.5 g/l Yeast extract 2.0 g/l CaCl2 x 2 H2O 0.25 g/l MgSO4 x 7 H2O 0.2 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water

culture temp

  • @ref: 2333
  • growth: positive
  • type: growth
  • temperature: 85
  • range: hyperthermophilic

Physiology and metabolism

compound production

@refcompound
2333alcohol dehydrogenase
2333aspartate aminotransferase
23332-keto-3 deoxygluconate
2333ß galactosidase
2333glucose
2333glucose dehydrogenase
2333malic enzyme
2333propylamine transferase
2333trehalose

Isolation, sampling and environmental information

isolation

  • @ref: 2333
  • sample type: solfatara
  • geographic location: Naples, Agnano, Pisciarelli
  • country: Italy
  • origin.country: ITA
  • continent: Europe

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Volcanic

Safety information

risk assessment

  • @ref: 2333
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

GC content

  • @ref: 2333
  • GC-content: 39.0

External links

@ref: 2333

culture collection no.: DSM 5833

straininfo link

  • @ref: 85674
  • straininfo: 48016

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1592111Isolation of a thermostable enzyme catalyzing disulfide bond formation from the archaebacterium Sulfolobus solfataricus.Guagliardi A, Cerchia L, De Rosa M, Rossi M, Bartolucci SFEBS Lett10.1016/0014-5793(92)80470-21992Amino Acid Sequence, Catalysis, Chromatography, Liquid, Disulfides/*metabolism, Electrophoresis, Polyacrylamide Gel, Enzyme Activation, Enzyme Stability, Hot Temperature, Molecular Sequence Data, Oxidation-Reduction, Oxidoreductases/isolation & purification/*metabolism, Oxidoreductases Acting on Sulfur Group Donors, Ribonucleases/metabolism, Sulfolobus/*enzymologyMetabolism
Enzymology2121622Isolation and sequencing of a new beta-galactosidase-encoding archaebacterial gene.Cubellis MV, Rozzo C, Montecucchi P, Rossi MGene10.1016/0378-1119(90)90472-41990Amino Acid Sequence, Archaea/enzymology/*genetics, Base Sequence, Cloning, Molecular, Codon/genetics, DNA, Bacterial/genetics/isolation & purification, *Genes, Bacterial, Molecular Sequence Data, Oligonucleotide Probes, Restriction Mapping, beta-Galactosidase/*geneticsPhylogeny
Enzymology3108257Malic enzyme from archaebacterium Sulfolobus solfataricus. Purification, structure, and kinetic properties.Bartolucci S, Rella R, Guagliardi A, Raia CA, Gambacorta A, De Rosa M, Rossi MJ Biol ChemS0021-9258(18)47628-41987Amino Acids/analysis, Archaea/*enzymology, Bacteria/*enzymology, Cations, Divalent, Enzyme Stability, Escherichia coli/enzymology, Kinetics, Macromolecular Substances, Malate Dehydrogenase/*isolation & purification/metabolism, Molecular Weight, ThermodynamicsPhylogeny
Enzymology3142524Oxalacetate decarboxylase and pyruvate carboxylase activities, and effect of sulfhydryl reagents in malic enzyme from Sulfolobus solfataricus.Guagliardi A, Moracci M, Manco G, Rossi M, Bartolucci SBiochim Biophys Acta10.1016/0167-4838(88)90287-71988Archaea/*enzymology, Bacteria/*enzymology, Carboxy-Lyases/*metabolism, Cations, Divalent/pharmacology, Hydrogen-Ion Concentration, Kinetics, Malate Dehydrogenase/antagonists & inhibitors/*metabolism, Malates/metabolism, NAD/pharmacology, NADP/pharmacology, Oxaloacetates/metabolism, Pyruvate Carboxylase/*metabolism, Substrate Specificity, Sulfhydryl Reagents/pharmacologyMetabolism
Enzymology8484908Exo-glucosidase activity and substrate specificity of the beta-glycosidase isolated from the extreme thermophile Sulfolobus solfataricus.Nucci R, Moracci M, Vaccaro C, Vespa N, Rossi MBiotechnol Appl Biochem1993Carbohydrate Sequence, Chromatography, Gel, Chromatography, Ion Exchange, Glycoside Hydrolases/antagonists & inhibitors/isolation & purification/*metabolism, Kinetics, Molecular Sequence Data, Substrate Specificity, Sulfolobus/*enzymologyGenetics
Genetics9654137Cloning and sequencing of ISC1041 from the archaeon Sulfolobus solfataricus MT-4, a new member of the IS30 family of insertion elements.Ammendola S, Politi L, Scandurra RFEBS Lett10.1016/s0014-5793(98)00530-41998Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA Primers, *DNA Transposable Elements, Evolution, Molecular, Genome, Bacterial, HIV Integrase/chemistry/genetics, HIV-1/genetics, Molecular Sequence Data, *Multigene Family, Phylogeny, Polymerase Chain Reaction, Sequence Alignment, Sequence Homology, Amino Acid, Sulfolobus/classification/*genetics, Transposases/chemistry/*geneticsEnzymology
Metabolism11064336Effective production of a thermostable alpha-glucosidase from Sulfolobus solfataricus in Escherichia coli exploiting a microfiltration bioreactor.Schiraldi C, Martino A, Acone M, Di Lernia I, Di Lazzaro A, Marulli F, Generoso M, Carteni M, De Rosa MBiotechnol Bioeng10.1002/1097-0290(20001220)70:6<670::aid-bit9>3.0.co;2-72000*Bioreactors, Biotechnology/*instrumentation/methods, Culture Media/metabolism, Escherichia coli/*metabolism, Fermentation, Lactose/metabolism, Plasmids/metabolism, Sulfolobus/*enzymology, Temperature, Thiogalactosides/metabolism, Time Factors, alpha-Glucosidases/*biosynthesisBiotechnology

Reference

@idauthorscataloguedoi/urltitle
2333Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5833)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5833
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
85674Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48016.1StrainInfo: A central database for resolving microbial strain identifiers