Strain identifier
BacDive ID: 16653
Type strain:
Species: Saccharolobus solfataricus
Strain Designation: MT-4
Strain history: <- A. Gambacorta <- A. Gambacorta and M. De Rosa, MT-4 ("Caldariella acidophila").
NCBI tax ID(s): 2287 (species)
General
@ref: 2333
BacDive-ID: 16653
DSM-Number: 5833
keywords: Archaea, hyperthermophilic
description: Saccharolobus solfataricus MT-4 is a hyperthermophilic archaeon that was isolated from solfatara.
NCBI tax id
- NCBI tax id: 2287
- Matching level: species
strain history
- @ref: 2333
- history: <- A. Gambacorta <- A. Gambacorta and M. De Rosa, MT-4 ("Caldariella acidophila").
doi: 10.13145/bacdive16653.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Sulfolobales
- family: Sulfolobaceae
- genus: Saccharolobus
- species: Saccharolobus solfataricus
- full scientific name: Saccharolobus solfataricus (Zillig et al. 1980) Sakai and Kurosawa 2018
synonyms
- @ref: 20215
- synonym: Sulfolobus solfataricus
@ref: 2333
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Sulfolobales
family: Sulfolobaceae
genus: Saccharolobus
species: Saccharolobus solfataricus
full scientific name: Saccharolobus solfataricus (Zillig et al. 1980) Sakai and Kurosawa 2018
strain designation: MT-4
type strain: no
Culture and growth conditions
culture medium
- @ref: 2333
- name: SACCHAROLOBUS (MT-4) MEDIUM (DSMZ Medium 182a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/182a
- composition: Name: SACCHAROLOBUS (MT-4) MEDIUM (DSMZ Medium 182a) Composition: KH2PO4 3.1 g/l (NH4)2SO4 2.5 g/l Yeast extract 2.0 g/l CaCl2 x 2 H2O 0.25 g/l MgSO4 x 7 H2O 0.2 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
culture temp
- @ref: 2333
- growth: positive
- type: growth
- temperature: 85
- range: hyperthermophilic
Physiology and metabolism
compound production
@ref | compound |
---|---|
2333 | alcohol dehydrogenase |
2333 | aspartate aminotransferase |
2333 | 2-keto-3 deoxygluconate |
2333 | ß galactosidase |
2333 | glucose |
2333 | glucose dehydrogenase |
2333 | malic enzyme |
2333 | propylamine transferase |
2333 | trehalose |
Isolation, sampling and environmental information
isolation
- @ref: 2333
- sample type: solfatara
- geographic location: Naples, Agnano, Pisciarelli
- country: Italy
- origin.country: ITA
- continent: Europe
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Volcanic
Safety information
risk assessment
- @ref: 2333
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
GC content
- @ref: 2333
- GC-content: 39.0
External links
@ref: 2333
culture collection no.: DSM 5833
straininfo link
- @ref: 85674
- straininfo: 48016
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Enzymology | 1592111 | Isolation of a thermostable enzyme catalyzing disulfide bond formation from the archaebacterium Sulfolobus solfataricus. | Guagliardi A, Cerchia L, De Rosa M, Rossi M, Bartolucci S | FEBS Lett | 10.1016/0014-5793(92)80470-2 | 1992 | Amino Acid Sequence, Catalysis, Chromatography, Liquid, Disulfides/*metabolism, Electrophoresis, Polyacrylamide Gel, Enzyme Activation, Enzyme Stability, Hot Temperature, Molecular Sequence Data, Oxidation-Reduction, Oxidoreductases/isolation & purification/*metabolism, Oxidoreductases Acting on Sulfur Group Donors, Ribonucleases/metabolism, Sulfolobus/*enzymology | Metabolism |
Enzymology | 2121622 | Isolation and sequencing of a new beta-galactosidase-encoding archaebacterial gene. | Cubellis MV, Rozzo C, Montecucchi P, Rossi M | Gene | 10.1016/0378-1119(90)90472-4 | 1990 | Amino Acid Sequence, Archaea/enzymology/*genetics, Base Sequence, Cloning, Molecular, Codon/genetics, DNA, Bacterial/genetics/isolation & purification, *Genes, Bacterial, Molecular Sequence Data, Oligonucleotide Probes, Restriction Mapping, beta-Galactosidase/*genetics | Phylogeny |
Enzymology | 3108257 | Malic enzyme from archaebacterium Sulfolobus solfataricus. Purification, structure, and kinetic properties. | Bartolucci S, Rella R, Guagliardi A, Raia CA, Gambacorta A, De Rosa M, Rossi M | J Biol Chem | S0021-9258(18)47628-4 | 1987 | Amino Acids/analysis, Archaea/*enzymology, Bacteria/*enzymology, Cations, Divalent, Enzyme Stability, Escherichia coli/enzymology, Kinetics, Macromolecular Substances, Malate Dehydrogenase/*isolation & purification/metabolism, Molecular Weight, Thermodynamics | Phylogeny |
Enzymology | 3142524 | Oxalacetate decarboxylase and pyruvate carboxylase activities, and effect of sulfhydryl reagents in malic enzyme from Sulfolobus solfataricus. | Guagliardi A, Moracci M, Manco G, Rossi M, Bartolucci S | Biochim Biophys Acta | 10.1016/0167-4838(88)90287-7 | 1988 | Archaea/*enzymology, Bacteria/*enzymology, Carboxy-Lyases/*metabolism, Cations, Divalent/pharmacology, Hydrogen-Ion Concentration, Kinetics, Malate Dehydrogenase/antagonists & inhibitors/*metabolism, Malates/metabolism, NAD/pharmacology, NADP/pharmacology, Oxaloacetates/metabolism, Pyruvate Carboxylase/*metabolism, Substrate Specificity, Sulfhydryl Reagents/pharmacology | Metabolism |
Enzymology | 8484908 | Exo-glucosidase activity and substrate specificity of the beta-glycosidase isolated from the extreme thermophile Sulfolobus solfataricus. | Nucci R, Moracci M, Vaccaro C, Vespa N, Rossi M | Biotechnol Appl Biochem | 1993 | Carbohydrate Sequence, Chromatography, Gel, Chromatography, Ion Exchange, Glycoside Hydrolases/antagonists & inhibitors/isolation & purification/*metabolism, Kinetics, Molecular Sequence Data, Substrate Specificity, Sulfolobus/*enzymology | Genetics | |
Genetics | 9654137 | Cloning and sequencing of ISC1041 from the archaeon Sulfolobus solfataricus MT-4, a new member of the IS30 family of insertion elements. | Ammendola S, Politi L, Scandurra R | FEBS Lett | 10.1016/s0014-5793(98)00530-4 | 1998 | Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA Primers, *DNA Transposable Elements, Evolution, Molecular, Genome, Bacterial, HIV Integrase/chemistry/genetics, HIV-1/genetics, Molecular Sequence Data, *Multigene Family, Phylogeny, Polymerase Chain Reaction, Sequence Alignment, Sequence Homology, Amino Acid, Sulfolobus/classification/*genetics, Transposases/chemistry/*genetics | Enzymology |
Metabolism | 11064336 | Effective production of a thermostable alpha-glucosidase from Sulfolobus solfataricus in Escherichia coli exploiting a microfiltration bioreactor. | Schiraldi C, Martino A, Acone M, Di Lernia I, Di Lazzaro A, Marulli F, Generoso M, Carteni M, De Rosa M | Biotechnol Bioeng | 10.1002/1097-0290(20001220)70:6<670::aid-bit9>3.0.co;2-7 | 2000 | *Bioreactors, Biotechnology/*instrumentation/methods, Culture Media/metabolism, Escherichia coli/*metabolism, Fermentation, Lactose/metabolism, Plasmids/metabolism, Sulfolobus/*enzymology, Temperature, Thiogalactosides/metabolism, Time Factors, alpha-Glucosidases/*biosynthesis | Biotechnology |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
2333 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5833) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5833 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
85674 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48016.1 | StrainInfo: A central database for resolving microbial strain identifiers |