Strain identifier

BacDive ID: 16650

Type strain: Yes

Species: Saccharolobus solfataricus

Strain Designation: P1

Strain history: DSM 1616 <-- W. Zillig P1.

NCBI tax ID(s): 2287 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 831

BacDive-ID: 16650

DSM-Number: 1616

keywords: genome sequence, 16S sequence, Archaea, thermophilic

description: Saccharolobus solfataricus P1 is a thermophilic archaeon that was isolated from volcanic hot spring.

NCBI tax id

  • NCBI tax id: 2287
  • Matching level: species

strain history

@refhistory
831<- Replacement from W. Zillig (<- W. Zillig, P1)
67770DSM 1616 <-- W. Zillig P1.

doi: 10.13145/bacdive16650.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/thermoproteota
  • domain: Archaea
  • phylum: Thermoproteota
  • class: Thermoprotei
  • order: Sulfolobales
  • family: Sulfolobaceae
  • genus: Saccharolobus
  • species: Saccharolobus solfataricus
  • full scientific name: Saccharolobus solfataricus (Zillig et al. 1980) Sakai and Kurosawa 2018
  • synonyms

    • @ref: 20215
    • synonym: Sulfolobus solfataricus

@ref: 831

domain: Archaea

phylum: Crenarchaeota

class: Thermoprotei

order: Sulfolobales

family: Sulfolobaceae

genus: Saccharolobus

species: Saccharolobus solfataricus

full scientific name: Saccharolobus solfataricus (Zillig et al. 1980) Sakai and Kurosawa 2018

strain designation: P1

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 831
  • name: SACCHAROLOBUS SOLFATARICUS MEDIUM (DSMZ Medium 182)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/182
  • composition: Name: SACCHAROLOBUS SOLFATARICUS MEDIUM (DSMZ Medium 182) Composition: KH2PO4 3.1 g/l (NH4)2SO4 2.5 g/l Casamino acids 1.0 g/l Yeast extract 1.0 g/l CaCl2 x 2 H2O 0.25 g/l MgSO4 x 7 H2O 0.2 g/l Na2B4O7 x 10 H2O 0.0045 g/l MnCl2 x 4 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.00022 g/l CuCl2 x 2 H2O 5e-05 g/l VOSO4 x 2 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l CoSO4 x 7 H2O 1e-05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
831positivegrowth70thermophilic
67770positivegrowth80hyperthermophilic

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
831volcanic hot springCampi flegrei, Pisciarelli near AgnanoItalyITAEurope
67770Volcanic hot spring at Pisciarelli SolfataraItalyITAEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Environmental#Terrestrial#Volcanic
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 831
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Sulfolobus solfataricus 16S rRNA gene complete sequence and 23S rRNA gene 5' regionD264902488ena2287
20218S.solfataricus DNA for 16S ribosomal RNA gene, strain DSM 1616X904781436ena2287
831Saccharolobus solfataricus strain IFO 15331 16S ribosomal RNA, partial sequenceNR_0291271495nuccore2287

Genome sequences

  • @ref: 67770
  • description: Saccharolobus solfataricus P1
  • accession: GCA_900079115
  • assembly level: complete
  • database: ncbi
  • NCBI tax ID: 2287

GC content

@refGC-contentmethod
83136.0
6777034high performance liquid chromatography (HPLC)
6777040Buoyant density centrifugation (BD)
6777033.7thermal denaturation, midpoint method (Tm)
6777033.7high performance liquid chromatography (HPLC)
6777036.1high performance liquid chromatography (HPLC)
6777036thermal denaturation, midpoint method (Tm)
6777034thermal denaturation, midpoint method (Tm)

External links

@ref: 831

culture collection no.: DSM 1616, ATCC 35091, JCM 8930, IFO 15158, IFO 15331, NBRC 15158, NBRC 15331

straininfo link

  • @ref: 85671
  • straininfo: 40867

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7592428Isolation and cloning of a protein-serine/threonine phosphatase from an archaeon.Leng J, Cameron AJ, Buckel S, Kennelly PJJ Bacteriol10.1128/jb.177.22.6510-6517.19951995Amino Acid Sequence, Base Sequence, Cloning, Molecular, Conserved Sequence, Escherichia coli/genetics, Genes, Bacterial/*genetics, Molecular Sequence Data, Phosphoprotein Phosphatases/biosynthesis/*genetics/*isolation & purification, Phylogeny, Polymerase Chain Reaction/methods, Sequence Alignment, Sequence Analysis, Sequence Analysis, DNA, Sulfolobus/*enzymology/geneticsEnzymology
Enzymology16466637Purifications and characterizations of a ferredoxin and its related 2-oxoacid:ferredoxin oxidoreductase from the hyperthermophilic archaeon, Sulfolobus solfataricus P1.Park YJ, Yoo CB, Choi SY, Lee HBJ Biochem Mol Biol10.5483/bmbrep.2006.39.1.0462006Amino Acid Sequence, *Archaeal Proteins/chemistry/genetics/isolation & purification/metabolism, *Ferredoxins, Hydrogen-Ion Concentration, Molecular Sequence Data, Molecular Weight, *Pyruvate Synthase/chemistry/genetics/isolation & purification/metabolism, Sequence Homology, Amino Acid, Substrate Specificity, *Sulfolobus solfataricus/chemistry/enzymology, TemperaturePhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
831Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1616)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1616
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
85671Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40867.1StrainInfo: A central database for resolving microbial strain identifiers