Strain identifier
BacDive ID: 16648
Type strain: 
Species: Stygiolobus azoricus
Strain Designation: FC6
Strain history: DSM 6296 <-- A. Segerer and K. O. Stetter FC6.
NCBI tax ID(s): 1313299 (strain), 41675 (species)
version 8.1 (current version)
General
@ref: 2580
BacDive-ID: 16648
DSM-Number: 6296
keywords: genome sequence, 16S sequence, Archaea, anaerobe, spore-forming, hyperthermophilic, Gram-negative
description: Stygiolobus azoricus FC6 is an anaerobe, spore-forming, hyperthermophilic archaeon that was isolated from hot spring.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 41675 | species |
| 1313299 | strain |
strain history
| @ref | history |
|---|---|
| 2580 | <- A. Segerer and K.O. Stetter, FC6 |
| 67770 | DSM 6296 <-- A. Segerer and K. O. Stetter FC6. |
doi: 10.13145/bacdive16648.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Sulfolobales
- family: Sulfolobaceae
- genus: Stygiolobus
- species: Stygiolobus azoricus
- full scientific name: Stygiolobus azoricus Segerer et al. 1991
@ref: 2580
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Sulfolobales
family: Sulfolobaceae
genus: Stygiolobus
species: Stygiolobus azoricus
full scientific name: Stygiolobus azoricus Segerer et al. 1991
strain designation: FC6
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.962
Culture and growth conditions
culture medium
- @ref: 2580
- name: ACIDIANUS MEDIUM (ANAEROBIC) (DSMZ Medium 358a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/358a
- composition: Name: ACIDIANUS MEDIUM (ANAEROBIC) (DSMZ Medium 358a; with strain-specific modifications) Composition: Sulfur 4.9505 g/l (NH4)2SO4 1.28713 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l Yeast extract 0.19802 g/l CaCl2 x 2 H2O 0.0693069 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 2580 | positive | growth | 80 | hyperthermophilic | |
| 67770 | positive | growth | 80 | hyperthermophilic | |
| 69480 | thermophilic | 100 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 2580 | anaerobe | |
| 69480 | anaerobe | 98.445 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | no | 99.923 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country |
|---|---|---|---|---|
| 2580 | hot spring | Sao Miguel island, Furnas | Azores | PRT |
| 67770 | Furnas Caldeiras | São Miguel Island | Azores | PRT |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 2580
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | S.azoricus DNA for 16S ribosomal RNA gene | X90480 | 1416 | ena | 41675 |
| 2580 | Stygiolobus azoricus 16S rRNA gene, almost full-length sequence | D85520 | 1457 | ena | 41675 |
Genome sequences
- @ref: 66792
- description: Stygiolobus azoricus FC6
- accession: GCA_009729035
- assembly level: complete
- database: ncbi
- NCBI tax ID: 41675
GC content
| @ref | GC-content | method |
|---|---|---|
| 2580 | 38.0 | |
| 67770 | 38 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | no | 77.393 | no |
| flagellated | no | 96.045 | no |
| gram-positive | no | 95.239 | no |
| anaerobic | yes | 90.372 | no |
| aerobic | no | 88.78 | yes |
| halophile | no | 79.663 | no |
| spore-forming | no | 96.743 | no |
| thermophile | yes | 100 | yes |
| glucose-util | yes | 85.169 | no |
| glucose-ferment | no | 77.67 | no |
External links
@ref: 2580
culture collection no.: DSM 6296, JCM 9021
straininfo link
- @ref: 85669
- straininfo: 48145
literature
- topic: Phylogeny
- Pubmed-ID: 35951006
- title: Stygiolobus caldivivus sp. nov., a facultatively anaerobic hyperthermophilic archaeon isolated from the Unzen hot spring in Japan.
- authors: Sakai HD, Nakamura K, Kurosawa N
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.005486
- year: 2022
- mesh: Anaerobiosis, Archaea/genetics, Bacteria, Anaerobic/genetics, Bacterial Typing Techniques, Base Composition, DNA, Archaeal/genetics, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Hot Springs, Japan, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sulfolobaceae
- topic2: Transcriptome
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 2580 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6296) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-6296 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 85669 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48145.1 | StrainInfo: A central database for resolving microbial strain identifiers |