Strain identifier
BacDive ID: 16647
Type strain: 
Species: Metallosphaera prunae
Strain history: <- K. O. Stetter; Ron 12/II <- T. Fuchs and H. Huber
NCBI tax ID(s): 47304 (species)
version 8.1 (current version)
General
@ref: 3760
BacDive-ID: 16647
DSM-Number: 10039
keywords: genome sequence, 16S sequence, Archaea, thermophilic
description: Metallosphaera prunae DSM 10039 is a thermophilic archaeon that was isolated from smoldering slag heap, uranium mine.
NCBI tax id
- NCBI tax id: 47304
- Matching level: species
strain history
- @ref: 3760
- history: <- K. O. Stetter; Ron 12/II <- T. Fuchs and H. Huber
doi: 10.13145/bacdive16647.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Sulfolobales
- family: Sulfolobaceae
- genus: Metallosphaera
- species: Metallosphaera prunae
- full scientific name: Metallosphaera prunae Fuchs et al. 1996
@ref: 3760
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Sulfolobales
family: Sulfolobaceae
genus: Metallosphaera
species: Metallosphaera prunae
full scientific name: Metallosphaera prunae Fuchs et al. 1996
type strain: yes
Culture and growth conditions
culture medium
- @ref: 3760
- name: SULFOLOBUS MEDIUM (DSMZ Medium 88)
- growth: yes
- link: https://mediadive.dsmz.de/medium/88
- composition: Name: SULFOLOBUS MEDIUM (DSMZ Medium 88; with strain-specific modifications) Composition: Sulfur powder 4.9505 g/l (NH4)2SO4 1.28713 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l Yeast extract 0.19802 g/l CaCl2 x 2 H2O 0.0693069 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water
culture temp
- @ref: 3760
- growth: positive
- type: growth
- temperature: 75
- range: thermophilic
Isolation, sampling and environmental information
isolation
- @ref: 3760
- sample type: smoldering slag heap, uranium mine
- geographic location: Ronneburg
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Other | #Mine |
| #Engineered | #Waste | #Solid waste |
| #Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 3760
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 3760
- description: M.prunae DNA for 16S ribosomal RNA gene
- accession: X90482
- length: 1420
- database: ena
- NCBI tax ID: 47304
Genome sequences
- @ref: 66792
- description: Metallosphaera prunae strain Ron 12/II
- accession: 47304.5
- assembly level: complete
- database: patric
- NCBI tax ID: 47304
GC content
| @ref | GC-content | method |
|---|---|---|
| 3760 | 47 | high performance liquid chromatography (HPLC) |
| 3760 | 45 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| gram-positive | no | 96.56 | no |
| anaerobic | yes | 85.562 | no |
| halophile | no | 84.604 | no |
| spore-forming | no | 96.48 | no |
| glucose-util | yes | 81.131 | no |
| aerobic | no | 79.397 | no |
| flagellated | no | 97.744 | no |
| thermophile | yes | 100 | yes |
| motile | no | 84.313 | no |
| glucose-ferment | no | 83.207 | no |
External links
@ref: 3760
culture collection no.: DSM 10039, Ron 12/II
straininfo link
- @ref: 85668
- straininfo: 48738
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 11523893 | Metallosphaera sedula TA-2, a calditoglycerocaldarchaeol deletion strain of a thermoacidophilic archaeon. | Itoh YH, Kurosawa N, Uda I, Sugai A, Tanoue S, Itoh T, Horiuchi T, Itoh T | Extremophiles | 10.1007/s007920100195 | 2001 | Adaptation, Biological, Diglycerides/genetics, Gene Deletion, Glycolipids/genetics, *Sulfolobales/genetics/growth & development/metabolism, Temperature | |
| Phylogeny | 21057050 | Metallosphaera cuprina sp. nov., an acidothermophilic, metal-mobilizing archaeon. | Liu LJ, You XY, Guo X, Liu SJ, Jiang CY | Int J Syst Evol Microbiol | 10.1099/ijs.0.026591-0 | 2010 | Aerobiosis, Base Composition, Carboxylic Acids/*metabolism, Chemoautotrophic Growth, China, Cluster Analysis, DNA, Archaeal/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Flagella/physiology, Hot Springs/*microbiology, Hydrogen-Ion Concentration, Lipids/analysis, Locomotion, Metals/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfolobaceae/*classification/genetics/*isolation & purification/physiology, Sulfur Compounds/metabolism, Temperature | Metabolism |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 3760 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10039) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-10039 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 85668 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48738.1 | StrainInfo: A central database for resolving microbial strain identifiers |