Strain identifier
BacDive ID: 16646
Type strain: 
Species: Metallosphaera hakonensis
Strain Designation: HO1-1
Strain history: S. Takayanagi HO 1-1.
NCBI tax ID(s): 1293036 (strain), 79601 (species)
version 8.1 (current version)
General
@ref: 3197
BacDive-ID: 16646
DSM-Number: 7519
keywords: genome sequence, 16S sequence, Archaea, anaerobe, spore-forming, thermophilic, Gram-negative
description: Metallosphaera hakonensis HO1-1 is an anaerobe, spore-forming, thermophilic archaeon that was isolated from hot spring.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 79601 | species |
| 1293036 | strain |
strain history
| @ref | history |
|---|---|
| 3197 | <- S. Takayanagi; HO1-1 <- S. Takayanagi and K. Sugimori {1985} |
| 67770 | S. Takayanagi HO 1-1. |
doi: 10.13145/bacdive16646.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Sulfolobales
- family: Sulfolobaceae
- genus: Metallosphaera
- species: Metallosphaera hakonensis
- full scientific name: Metallosphaera hakonensis (Takayanagi et al. 1996) Kurosawa et al. 2003
synonyms
- @ref: 20215
- synonym: Sulfolobus hakonensis
@ref: 3197
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Sulfolobales
family: Sulfolobaceae
genus: Metallosphaera
species: Metallosphaera hakonensis
full scientific name: Metallosphaera hakonensis (Takayanagi et al. 1996) Kurosawa et al. 2003
strain designation: HO1-1
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.847
colony morphology
- @ref: 3197
- incubation period: 3-7 days
Culture and growth conditions
culture medium
- @ref: 3197
- name: SULFOLOBUS MEDIUM (DSMZ Medium 88)
- growth: yes
- link: https://mediadive.dsmz.de/medium/88
- composition: Name: SULFOLOBUS MEDIUM (DSMZ Medium 88) Composition: (NH4)2SO4 1.28713 g/l Yeast extract 0.990099 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l CaCl2 x 2 H2O 0.0693069 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 3197 | positive | growth | 65 | thermophilic | |
| 67770 | positive | growth | 70 | thermophilic | |
| 69480 | thermophilic | 100 |
Physiology and metabolism
oxygen tolerance
- @ref: 69480
- oxygen tolerance: anaerobe
- confidence: 98.651
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | no | 99.936 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 3197 | hot spring | Kanagawa, Ohwakudani area, Hakone Natl. Park | Japan | JPN | Asia |
| 67770 | Lava field at Owakudani | Hakone | Japan | JPN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_194387.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17817;96_68846;97_93095;98_126653;99_194387&stattab=map
- Last taxonomy: Metallosphaera hakonensis subclade
- 16S sequence: D86414
- Sequence Identity:
- Total samples: 7
- aquatic counts: 7
Safety information
risk assessment
- @ref: 3197
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Metallosphaera hakonensis gene for 16S rRNA, partial sequence | D86414 | 1461 | ena | 79601 |
| 3197 | Sulfolobus sp. H 16S rRNA | D14052 | 1446 | ena | 359275 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Metallosphaera hakonensis JCM 8857 = DSM 7519 HO1-1 | GCA_003201675 | complete | ncbi | 1293036 |
| 66792 | Metallosphaera hakonensis JCM 8857 = DSM 7519 | GCA_001315825 | contig | ncbi | 1293036 |
| 66792 | Metallosphaera hakonensis JCM 8857 | 2681813025 | draft | img | 1293036 |
| 66792 | Metallosphaera hakonensis strain HO1-1 | 79601.6 | complete | patric | 79601 |
| 66792 | Metallosphaera hakonensis JCM 8857 | 1293036.3 | wgs | patric | 1293036 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 3197 | 46.2 | high performance liquid chromatography (HPLC) |
| 67770 | 38.4 | Buoyant density centrifugation (BD) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| flagellated | no | 97.706 | no |
| flagellated | no | 97.706 | no |
| gram-positive | no | 94.411 | no |
| gram-positive | no | 94.411 | no |
| anaerobic | yes | 80.071 | no |
| anaerobic | yes | 80.071 | no |
| halophile | no | 82.332 | no |
| halophile | no | 82.332 | no |
| spore-forming | no | 95.964 | no |
| spore-forming | no | 95.964 | no |
| glucose-util | yes | 85.132 | no |
| glucose-util | yes | 85.132 | no |
| aerobic | no | 75.164 | no |
| aerobic | no | 75.164 | no |
| thermophile | yes | 100 | no |
| thermophile | yes | 100 | no |
| motile | no | 83.611 | no |
| motile | no | 83.611 | no |
| glucose-ferment | no | 81.218 | no |
| glucose-ferment | no | 81.218 | no |
External links
@ref: 3197
culture collection no.: DSM 7519, JCM 8857, ATCC 51241, IAM 14250
straininfo link
- @ref: 85667
- straininfo: 42873
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 13130056 | Reclassification of Sulfolobus hakonensis Takayanagi et al. 1996 as Metallosphaera hakonensis comb. nov. based on phylogenetic evidence and DNA G+C content. | Kurosawa N, Itoh YH, Itoh T | Int J Syst Evol Microbiol | 10.1099/ijs.0.02716-0 | 2003 | Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Ferrous Compounds/metabolism, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sulfolobaceae/*classification/genetics/metabolism, Sulfolobus/*classification/genetics/metabolism, Tetrathionic Acid/metabolism | Metabolism |
| Phylogeny | 21057050 | Metallosphaera cuprina sp. nov., an acidothermophilic, metal-mobilizing archaeon. | Liu LJ, You XY, Guo X, Liu SJ, Jiang CY | Int J Syst Evol Microbiol | 10.1099/ijs.0.026591-0 | 2010 | Aerobiosis, Base Composition, Carboxylic Acids/*metabolism, Chemoautotrophic Growth, China, Cluster Analysis, DNA, Archaeal/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Flagella/physiology, Hot Springs/*microbiology, Hydrogen-Ion Concentration, Lipids/analysis, Locomotion, Metals/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfolobaceae/*classification/genetics/*isolation & purification/physiology, Sulfur Compounds/metabolism, Temperature | Metabolism |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 3197 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7519) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-7519 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 85667 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42873.1 | StrainInfo: A central database for resolving microbial strain identifiers |