Strain identifier
BacDive ID: 16645
Type strain: 
Species: Metallosphaera sedula
Strain Designation: TH2
Strain history: <- K.O. Stetter and G. Huber, TH2
NCBI tax ID(s): 399549 (strain), 43687 (species)
version 8.1 (current version)
General
@ref: 1995
BacDive-ID: 16645
DSM-Number: 5348
keywords: genome sequence, 16S sequence, Archaea, thermophilic
description: Metallosphaera sedula TH2 is a thermophilic archaeon that was isolated from hot water pond.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 399549 | strain |
| 43687 | species |
strain history
- @ref: 1995
- history: <- K.O. Stetter and G. Huber, TH2
doi: 10.13145/bacdive16645.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Sulfolobales
- family: Sulfolobaceae
- genus: Metallosphaera
- species: Metallosphaera sedula
- full scientific name: Metallosphaera sedula Huber et al. 1989
@ref: 1995
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Sulfolobales
family: Sulfolobaceae
genus: Metallosphaera
species: Metallosphaera sedula
full scientific name: Metallosphaera sedula Huber et al. 1989
strain designation: TH2
type strain: yes
Morphology
colony morphology
- @ref: 1995
- incubation period: 3-7 days
Culture and growth conditions
culture medium
- @ref: 1995
- name: SULFOLOBUS MEDIUM (DSMZ Medium 88)
- growth: yes
- link: https://mediadive.dsmz.de/medium/88
- composition: Name: SULFOLOBUS MEDIUM (DSMZ Medium 88; with strain-specific modifications) Composition: None 19.802 g/l (NH4)2SO4 1.28713 g/l Sulfur powder 0.49505 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l CaCl2 x 2 H2O 0.0693069 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water
culture temp
- @ref: 1995
- growth: positive
- type: growth
- temperature: 65
- range: thermophilic
Isolation, sampling and environmental information
isolation
- @ref: 1995
- sample type: hot water pond
- geographic location: Naples, Pisciarelli Solfatara
- country: Italy
- origin.country: ITA
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Pond (small) |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_187187.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17817;96_68846;97_89491;98_121771;99_187187&stattab=map
- Last taxonomy: Metallosphaera
- 16S sequence: D26491
- Sequence Identity:
- Total samples: 30
- aquatic counts: 29
- animal counts: 1
Safety information
risk assessment
- @ref: 1995
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Metallosphaera sedula 16S rRNA gene complete sequence and 23S rRNA gene 5' region | D26491 | 2849 | ena | 399549 |
| 20218 | M.sedula DNA for 16S ribosomal RNA gene | X90481 | 1416 | ena | 399549 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Metallosphaera sedula DSM 5348 | 640427120 | complete | img | 399549 |
| 66792 | Metallosphaera sedula DSM 5348 | 399549.15 | complete | patric | 399549 |
GC content
- @ref: 1995
- GC-content: 45.0
External links
@ref: 1995
culture collection no.: DSM 5348, ATCC 51363
straininfo link
- @ref: 85666
- straininfo: 42950
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 11523893 | Metallosphaera sedula TA-2, a calditoglycerocaldarchaeol deletion strain of a thermoacidophilic archaeon. | Itoh YH, Kurosawa N, Uda I, Sugai A, Tanoue S, Itoh T, Horiuchi T, Itoh T | Extremophiles | 10.1007/s007920100195 | 2001 | Adaptation, Biological, Diglycerides/genetics, Gene Deletion, Glycolipids/genetics, *Sulfolobales/genetics/growth & development/metabolism, Temperature | |
| Genetics | 18083856 | The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism. | Auernik KS, Maezato Y, Blum PH, Kelly RM | Appl Environ Microbiol | 10.1128/AEM.02019-07 | 2007 | Amino Acid Sequence, Archaeal Proteins/genetics/*metabolism, Gene Expression Regulation, Archaeal, *Genome, Archaeal, *Hot Temperature, Iron/*metabolism, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Oxidation-Reduction, *Sequence Analysis, DNA, Sulfates/*metabolism, *Sulfolobaceae/classification/genetics/metabolism | Metabolism |
| Phylogeny | 21057050 | Metallosphaera cuprina sp. nov., an acidothermophilic, metal-mobilizing archaeon. | Liu LJ, You XY, Guo X, Liu SJ, Jiang CY | Int J Syst Evol Microbiol | 10.1099/ijs.0.026591-0 | 2010 | Aerobiosis, Base Composition, Carboxylic Acids/*metabolism, Chemoautotrophic Growth, China, Cluster Analysis, DNA, Archaeal/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Flagella/physiology, Hot Springs/*microbiology, Hydrogen-Ion Concentration, Lipids/analysis, Locomotion, Metals/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfolobaceae/*classification/genetics/*isolation & purification/physiology, Sulfur Compounds/metabolism, Temperature | Metabolism |
| Metabolism | 29692683 | Inorganic Polyphosphate, Exopolyphosphatase, and Pho84-Like Transporters May Be Involved in Copper Resistance in Metallosphaera sedula DSM 5348(T). | Rivero M, Torres-Paris C, Munoz R, Cabrera R, Navarro CA, Jerez CA | Archaea | 10.1155/2018/5251061 | 2018 | Acid Anhydride Hydrolases/*metabolism, Copper/metabolism/*toxicity, Cytoplasmic Granules/metabolism, *Drug Resistance, Microbial, Gene Expression Profiling, Membrane Transport Proteins/*metabolism, Mutagenesis, Site-Directed, Polyphosphates/*metabolism, Sulfolobaceae/*drug effects/*enzymology/genetics/metabolism, Trace Elements/metabolism/toxicity | Enzymology |
| 32195286 | Identified Hybrid tRNA Structure Genes in Archaeal Genome. | Mandal UR, Das SS, Chattopadhyay B, Sahoo S | Iran J Biotechnol | 10.29252/ijb.2254 | 2019 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1995 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5348) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5348 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 85666 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42950.1 | StrainInfo: A central database for resolving microbial strain identifiers |