Strain identifier

BacDive ID: 16626

Type strain: Yes

Species: Sphaerisporangium flaviroseum

Strain history: <- Y Cao, Yunnan Inst., China

NCBI tax ID(s): 509199 (species)

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General

@ref: 15457

BacDive-ID: 16626

DSM-Number: 45170

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Sphaerisporangium flaviroseum DSM 45170 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 509199
  • Matching level: species

strain history

@refhistory
15457<- S.-K. Tang, YIM; YIM 48771 <- Y.-R. Cao et al.
67770DSM 45170 <-- S.-K. Tang <-- Y.-R. Cao et al. YIM 48771.
67771<- Y Cao, Yunnan Inst., China

doi: 10.13145/bacdive16626.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Streptosporangiaceae
  • genus: Sphaerisporangium
  • species: Sphaerisporangium flaviroseum
  • full scientific name: Sphaerisporangium flaviroseum Cao et al. 2009

@ref: 15457

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptosporangiaceae

genus: Sphaerisporangium

species: Sphaerisporangium flaviroseum

full scientific name: Sphaerisporangium flaviroseum Cao et al. 2009

type strain: yes

Morphology

cell morphology

@refgram stain
29096positive
67771positive

colony morphology

@refcolony colorincubation periodmedium used
19919Zinc yellow10-14 daysISP 6
19919Light ivory10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19919noISP 6
19919noISP 7

pigmentation

  • @ref: 29096
  • production: yes

multimedia

  • @ref: 15457
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45170.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15457GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19919ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19919ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
15457ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
15457STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15457positivegrowth28mesophilic
19919positiveoptimum28mesophilic
67770positivegrowth28mesophilic
67771positivegrowth28mesophilic

culture pH

@refabilitytypepH
29096positivegrowth06-08
29096positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29096aerobe
67771aerobe

spore formation

  • @ref: 29096
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
29096NaClpositivegrowth<1 %
29096NaClpositiveoptimum1 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4), MK-9(H2), MK-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1991917234glucose+
1991922599arabinose+/-
1991917992sucrose+/-
1991918222xylose+/-
1991917268myo-inositol+/-
1991929864mannitol+
1991928757fructose+
1991926546rhamnose+/-
1991916634raffinose-
1991962968cellulose+/-
2909622599arabinose+carbon source
2909629016arginine+carbon source
2909623652dextrin+carbon source
2909628757fructose+carbon source
2909633984fucose+carbon source
2909628260galactose+carbon source
2909617234glucose+carbon source
2909617716lactose+carbon source
2909617306maltose+carbon source
2909629864mannitol+carbon source
2909637684mannose+carbon source
2909628053melibiose+carbon source
2909628044phenylalanine+carbon source
2909626271proline+carbon source
2909616634raffinose+carbon source
2909626546rhamnose+carbon source
2909633942ribose+carbon source
2909617822serine+carbon source
2909617992sucrose+carbon source
2909616199urea+carbon source
2909618222xylose+carbon source
290964853esculin+hydrolysis

enzymes

@refvalueactivityec
29096acid phosphatase+3.1.3.2
29096alkaline phosphatase+3.1.3.1
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase+3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19919+++++++++++++-+++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15457soilHunan ProvinceChinaCHNAsia
67771From Hunan ProvinceChinaCHNAsia
67770Soil from virgin forest at Jinbian RivuletHunan ProvinceChinaCHNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 15457
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15457
  • description: Sphaerisporangium flaviroseum strain YIM 48771 16S ribosomal RNA gene, partial sequence
  • accession: EU499338
  • length: 1400
  • database: ena
  • NCBI tax ID: 509199

GC content

@refGC-contentmethod
1545767.1high performance liquid chromatography (HPLC)
2909667.1

External links

@ref: 15457

culture collection no.: DSM 45170, BCC 39201, JCM 16908, KCTC 19393, TBRC 576, YIM 48771

straininfo link

  • @ref: 85647
  • straininfo: 400048

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19542141Sphaerisporangium flaviroseum sp. nov. and Sphaerisporangium album sp. nov., isolated from forest soil in China.Cao YR, Jiang Y, Xu LH, Jiang CLInt J Syst Evol Microbiol10.1099/ijs.0.006130-02009Actinomycetales/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Species Specificity, *TreesGenetics
Phylogeny25963939Sphaerisporangium corydalis sp. nov., isolated from the root of Corydalis yanhusuo L.Wang X, Liu C, Cheng J, Zhang Y, Ma Z, Li L, Wang X, Xiang WAntonie Van Leeuwenhoek10.1007/s10482-015-0471-x2015Actinobacteria/*classification/genetics/*isolation & purification/physiology, Aerobiosis, Cell Wall/chemistry, China, Cluster Analysis, Corydalis/*microbiology, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, Phylogeny, Plant Roots/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analysisGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15457Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45170)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45170
19919Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45170.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29096Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2552528776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68382Automatically annotated from API zym
85647Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID400048.1StrainInfo: A central database for resolving microbial strain identifiers