Strain identifier

BacDive ID: 16557

Type strain: Yes

Species: Nonomuraea roseoviolacea

Culture col. no.: DSM 43144, ATCC 27297, KCC A-0145

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 10815

BacDive-ID: 16557

DSM-Number: 43144

keywords: spore-forming, Bacteria, 16S sequence, mesophilic

description: Nonomuraea roseoviolacea DSM 43144 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

strain history: <- KCC <- H. Nonomura, FYU, A-5

doi: 10.13145/bacdive16557.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Streptosporangiales
  • family: Streptosporangiaceae
  • genus: Nonomuraea
  • species: Nonomuraea roseoviolacea
  • full scientific name: Nonomuraea roseoviolacea corrig. (Nonomura and Ohara 1971) Zhang et al. 1998
  • synonyms

    @refsynonym
    20215Actinomadura roseoviolacea
    20215Microtetraspora roseoviolacea

@ref: 10815

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptosporangiaceae

genus: Nonomuraea

species: Nonomuraea roseoviolacea

full scientific name: Nonomuraea roseoviolacea (Nonomura and Ohara 1971) Zhang et al. 1998

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19547Ruby red10-14 daysISP 2
19547Red10-14 daysISP 3
19547Red10-14 daysISP 4
19547Orange10-14 daysISP 5
19547Brown orange10-14 daysISP 6
19547Brown orange10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19547yesAerial MyceliumWhiteISP 2
19547yesAerial MyceliumWhiteISP 3
19547yesAerial MyceliumWhiteISP 4
19547noISP 5
19547noISP 6
19547noISP 7

multimedia

  • @ref: 10815
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43144.jpg
  • caption: Medium 65 28°C
  • license or copyright: (C) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10815GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
19547ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19547ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19547ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19547ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19547ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19547ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
40605MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)

culture temp

@refgrowthtypetemperaturerange
10815positivegrowth28mesophilic
19547positiveoptimum37mesophilic
40605positivegrowth37mesophilic

Physiology and metabolism

spore formation

  • @ref: 19547
  • spore description: Formation of spore chains: closed spirals, spore surface: smooth, sporangia: pseudosporangia
  • type of spore: spore
  • spore formation: yes

halophily

  • @ref: 19547
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19547++++++-+-++++-+-+++

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19547-+++--+--++

Isolation, sampling and environmental information

isolation

  • @ref: 10815
  • sample type: soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
108151Risk group (German classification)
195471Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Nonomuraea roseoviolacea gene for 16S ribosomal RNA, partial sequence, strain:IFO 14098TAB0399591453ena103837
20218Nonomuraea roseoviolacea gene for 16S rRNA, partial sequenceAB0431011472ena103837

External links

@ref: 10815

culture collection no.: DSM 43144, ATCC 27297, KCC A-0145

straininfo link

@refpassport
20218http://www.straininfo.net/strains/153503
20218http://www.straininfo.net/strains/105151
20218http://www.straininfo.net/strains/153510

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10815Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43144)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43144
19547Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43144.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40605Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19108