Strain identifier
BacDive ID: 16557
Type strain:
Species: Nonomuraea roseoviolacea
Strain Designation: A-5
Strain history: CIP <- 2001, CCM <- KCC, Actinomadura roseoviolacea, H. Nonomura: strain A-5
NCBI tax ID(s): 103837 (species)
General
@ref: 10815
BacDive-ID: 16557
DSM-Number: 43144
keywords: 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive, rod-shaped
description: Nonomuraea roseoviolacea A-5 is a spore-forming, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from soil.
NCBI tax id
- NCBI tax id: 103837
- Matching level: species
strain history
@ref | history |
---|---|
10815 | <- KCC <- H. Nonomura, FYU, A-5 |
67770 | KCC A-0145 <-- H. Nonomura A-5. |
122261 | CIP <- 2001, CCM <- KCC, Actinomadura roseoviolacea, H. Nonomura: strain A-5 |
doi: 10.13145/bacdive16557.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Streptosporangiales
- family: Streptosporangiaceae
- genus: Nonomuraea
- species: Nonomuraea roseoviolacea
- full scientific name: Nonomuraea roseoviolacea corrig. (Nonomura and Ohara 1971) Zhang et al. 1998
synonyms
@ref synonym 20215 Actinomadura roseoviolacea 20215 Nonomuria roseoviolacea 20215 Microtetraspora roseoviolacea
@ref: 10815
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptosporangiaceae
genus: Nonomuraea
species: Nonomuraea roseoviolacea subsp. roseoviolacea
full scientific name: Nonomuraea roseoviolacea subsp. roseoviolacea (Nonomura and Ohara 1971) Gyobu and Miyadoh 2001
strain designation: A-5
type strain: yes
Morphology
cell morphology
- @ref: 122261
- gram stain: positive
- cell shape: rod-shaped
- motility: no
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19547 | Ruby red | 10-14 days | ISP 2 |
19547 | Red | 10-14 days | ISP 3 |
19547 | Red | 10-14 days | ISP 4 |
19547 | Orange | 10-14 days | ISP 5 |
19547 | Brown orange | 10-14 days | ISP 6 |
19547 | Brown orange | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
19547 | yes | Aerial Mycelium | White | ISP 2 |
19547 | yes | Aerial Mycelium | White | ISP 3 |
19547 | yes | Aerial Mycelium | White | ISP 4 |
19547 | no | ISP 5 | ||
19547 | no | ISP 6 | ||
19547 | no | ISP 7 |
multimedia
- @ref: 10815
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43144.jpg
- caption: Medium 65 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
10815 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
19547 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
19547 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
19547 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
19547 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
19547 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
19547 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
40605 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
10815 | CZAPEK PEPTONE AGAR (DSMZ Medium 83) | yes | https://mediadive.dsmz.de/medium/83 | Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water |
122261 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
10815 | positive | growth | 28 | mesophilic |
19547 | positive | optimum | 37 | mesophilic |
40605 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
122261 | positive | growth | 5-41 |
Physiology and metabolism
spore formation
- @ref: 19547
- spore description: Formation of spore chains: closed spirals, spore surface: smooth, sporangia: pseudosporangia
- type of spore: spore
- spore formation: yes
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
19547 | NaCl | positive | maximum | 2.5 % |
122261 | NaCl | positive | growth | 0 % |
122261 | NaCl | no | growth | 2 % |
122261 | NaCl | no | growth | 4 % |
122261 | NaCl | no | growth | 6 % |
122261 | NaCl | no | growth | 8 % |
122261 | NaCl | no | growth | 10 % |
observation
- @ref: 67770
- observation: quinones: MK-9(H4), MK-9(H4), MK-9(H2), MK-9(III,VIII-H4)
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | + | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | + | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | + | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | - | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | + | builds acid from | 17057 |
68371 | salicin | + | builds acid from | 17814 |
68371 | esculin | - | builds acid from | 4853 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | + | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | - | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | + | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | - | builds acid from | 16024 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | + | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | + | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | L-arabinose | + | builds acid from | 30849 |
68371 | D-arabinose | + | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | + | builds acid from | 17754 |
68368 | gelatin | + | hydrolysis | 5291 |
68368 | tryptophan | - | energy source | 27897 |
68368 | urea | + | hydrolysis | 16199 |
68368 | citrate | - | assimilation | 16947 |
68368 | ornithine | + | degradation | 18257 |
68368 | lysine | + | degradation | 25094 |
68368 | arginine | + | hydrolysis | 29016 |
122261 | esculin | - | hydrolysis | 4853 |
122261 | nitrate | + | reduction | 17632 |
122261 | nitrite | + | reduction | 16301 |
antibiotic resistance
- @ref: 122261
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
67770 | 49322 | anthracycline antibiotic | yes |
68368 | 15688 | acetoin | yes |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
122261 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test | methylred-test |
---|---|---|---|---|---|
68368 | 15688 | acetoin | + | ||
68368 | 35581 | indole | - | ||
122261 | 15688 | acetoin | - | ||
122261 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68368 | gelatinase | + | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | + | 3.5.1.5 |
68368 | ornithine decarboxylase | + | 4.1.1.17 |
68368 | lysine decarboxylase | + | 4.1.1.18 |
68368 | arginine dihydrolase | + | 3.5.3.6 |
68368 | beta-galactosidase | - | 3.2.1.23 |
122261 | oxidase | - | |
122261 | beta-galactosidase | + | 3.2.1.23 |
122261 | alcohol dehydrogenase | - | 1.1.1.1 |
122261 | gelatinase | - | |
122261 | amylase | - | |
122261 | DNase | - | |
122261 | caseinase | - | 3.4.21.50 |
122261 | catalase | + | 1.11.1.6 |
122261 | tween esterase | - | |
122261 | gamma-glutamyltransferase | + | 2.3.2.2 |
122261 | lecithinase | - | |
122261 | lipase | - | |
122261 | lysine decarboxylase | - | 4.1.1.18 |
122261 | ornithine decarboxylase | - | 4.1.1.17 |
122261 | phenylalanine ammonia-lyase | - | 4.3.1.24 |
122261 | protease | - | |
122261 | tryptophan deaminase | - | |
122261 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | Control |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19547 | + | + | + | + | + | + | - | + | - | + | + | + | + | - | + | - | + | + | + | |
122261 | + | - | - | - | + | - | - | - | - | + | + | - | - | - | + | - | + | - | - | - |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
---|---|---|---|---|---|---|---|---|---|---|---|
19547 | - | + | + | + | - | - | + | - | - | + | + |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
122261 | + | - | + | + | +/- | - | - | + | - | + | + | +/- | - | - | + | - | - | - | - | - | + | + | - | - | - | + | + | + | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
10815 | soil | ||||
67770 | Soil, dry-heated | Yamanashi Pref. | Japan | JPN | Asia |
122261 | Environment, Soil | Japan | JPN | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
10815 | 1 | Risk group (German classification) |
19547 | 1 | Risk group (German classification) |
122261 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Nonomuraea roseoviolacea gene for 16S ribosomal RNA, partial sequence, strain:IFO 14098T | AB039959 | 1453 | ena | 103837 |
20218 | Nonomuraea roseoviolacea gene for 16S rRNA, partial sequence | AB043101 | 1472 | ena | 103837 |
GC content
@ref | GC-content | method |
---|---|---|
67770 | 68.5 | thermal denaturation, midpoint method (Tm) |
67770 | 68 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 10815
culture collection no.: DSM 43144, ATCC 27297, KCC A-0145, JCM 3145, BCRC 13406, CBS 260.72, CCM 3491, CGMCC 4.1072, CIP 106924, IFO 14098, IMET 9751, IMSNU 20098, KCTC 9251, KCTC 9283, MTCC 1396, NBRC 14098, NCIMB 11117, NRRL B-16127, VKM Ac-909, CCRC 13406, VKM Ac-90
straininfo link
- @ref: 85585
- straininfo: 38920
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 11411710 | Proposal to transfer Actinomadura carminata to a new subspecies of the genus Nonomuraea as Nonomuraea roseoviolacea subsp. carminata comb. nov. | Gyobu Y, Miyadoh S | Int J Syst Evol Microbiol | 10.1099/00207713-51-3-881 | 2001 | Actinomycetales/*classification/physiology/ultrastructure, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fermentation, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics | Genetics |
Phylogeny | 24801152 | Nonomuraea muscovyensis sp. nov., isolated from soil. | Ozdemir-Kocak F, Isik K, Veyisoglu A, Tatar D, Sahin N | Int J Syst Evol Microbiol | 10.1099/ijs.0.061291-0 | 2014 | Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, *Soil Microbiology, Trees/*microbiology, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
Phylogeny | 33595432 | Nonomuraea montanisoli sp. nov., isolated from mountain forest soil. | Chanama S, Suriyachadkun C, Chanama M | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004695 | 2021 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
10815 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43144) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-43144 | |||
19547 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM43144.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
40605 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/19108 | ||||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
85585 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID38920.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
122261 | Curators of the CIP | Collection of Institut Pasteur (CIP 106924) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106924 |