Strain identifier

BacDive ID: 16547

Type strain: Yes

Species: Nonomuraea angiospora

Strain Designation: LIA-0443

Culture col. no.: DSM 43173, 3479 , IFO 13155, KCC A-0109, NBRC 13155

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 10841

BacDive-ID: 16547

DSM-Number: 43173

keywords: Bacteria, 16S sequence, genome sequence, mesophilic

description: Nonomuraea angiospora LIA-0443 is a mesophilic bacterium that builds an aerial mycelium and was isolated from soil, a vineyard in the Georgian.

strain history: <- KCC (Actinomadura angiospora) <- Yu.E. Konev, LIA <- R. A. Zhukova, 3479/30

doi: 10.13145/bacdive16547.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Streptosporangiales
  • family: Streptosporangiaceae
  • genus: Nonomuraea
  • species: Nonomuraea angiospora
  • full scientific name: Nonomuraea angiospora corrig. (Zhukova et al. 1968) Zhang et al. 1998
  • synonyms

    @refsynonym
    20215Micropolyspora angiospora
    20215Microtetraspora angiospora

@ref: 10841

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptosporangiaceae

genus: Nonomuraea

species: Nonomuraea angiospora

full scientific name: Nonomuraea angiospora (Zhukova et al. 1968) Zhang et al. 1998

strain designation: LIA-0443

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19944Beige (1001)10-14 daysISP 2
19944Colorless10-14 daysISP 3
19944Lemon yellow (1012)10-14 daysISP 4
19944Lemon yellow (1012)10-14 daysISP 5
19944Beige (1001)10-14 daysISP 6
19944Green brown (8000)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19944yesAerial MyceliumSparse/ whiteISP 2
19944yesAerial MyceliumWhiteISP 3
19944yesAerial MyceliumWhiteISP 4
19944noISP 5
19944noISP 6
19944noISP 7

multimedia

  • @ref: 10841
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43173.jpg
  • caption: Medium 65 28°C
  • license or copyright: (C) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10841GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
19944ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19944ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19944ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19944ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19944ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19944ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33114MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)

culture temp

@refgrowthtypetemperaturerange
19944positiveoptimum37mesophilic
10841positivegrowth28mesophilic
33114positivegrowth30mesophilic

Physiology and metabolism

halophily

  • @ref: 19944
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19944+-+-+--++-++--+-+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19944-+++--+---+

Isolation, sampling and environmental information

isolation

  • @ref: 10841
  • sample type: soil, a vineyard in the Georgian
  • country: USSR
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Vineyard
#Environmental#Terrestrial#Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
199441German classification
108411Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Microtetraspora angiospora 16S rRNA gene
  • accession: U48843
  • length: 1411
  • database: ena
  • NCBI tax ID: 46172

Genome sequences

  • @ref: 66792
  • description: Nonomuraea angiospora DSM 43173
  • accession: 2873472684
  • assembly level: permanent
  • database: img
  • NCBI tax ID: 46172

External links

@ref: 10841

culture collection no.: DSM 43173, 3479, IFO 13155, KCC A-0109, NBRC 13155

straininfo link

@refpassport
20218http://www.straininfo.net/strains/167999
20218http://www.straininfo.net/strains/168000
20218http://www.straininfo.net/strains/168001
20218http://www.straininfo.net/strains/333421

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10841Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43173)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43173
19944Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43173.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33114Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4784
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)