Strain identifier
BacDive ID: 16498
Type strain:
Species: Streptomyces pratens
Strain Designation: BK138
Strain history: CGMCC 4.5800 <-- B.-Y. Kim et al. BK138.
NCBI tax ID(s): 887456 (species)
General
@ref: 18063
BacDive-ID: 16498
DSM-Number: 42061
keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive
description: Streptomyces pratens BK138 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil of a hay meadow.
NCBI tax id
- NCBI tax id: 887456
- Matching level: species
strain history
@ref | history |
---|---|
18063 | <- S. W. Kwon, KACC; KACC 20904 |
67770 | CGMCC 4.5800 <-- B.-Y. Kim et al. BK138. |
doi: 10.13145/bacdive16498.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces pratens
- full scientific name: Streptomyces pratens Kim et al. 2012
@ref: 18063
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces pratens
full scientific name: Streptomyces pratens Kim et al. 2012
strain designation: BK138
type strain: yes
Morphology
cell morphology
- @ref: 30450
- gram stain: positive
colony morphology
@ref | colony color | medium used |
---|---|---|
69335 | Ivory (1014) | ISP 2 |
69335 | Ivory (1014) | ISP 5 |
69335 | Light ivory (1015), oyster white (1013) | ISP 7 |
69335 | Ochre yellow (1024) | ISP 6 |
69335 | Oyster white (1013) | ISP 4 |
69335 | Sand yellow (1002) | suter without tyrosine |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
69335 | yes | Aerial mycelium | Traffic white (9016) | ISP 2 |
69335 | yes | Aerial mycelium | Yellow grey (7034), traffic white (9016) | ISP 3 |
69335 | yes | Aerial mycelium | Grey white (9002) | ISP 4 |
69335 | yes | Aerial mycelium | ISP 5 | |
69335 | yes | Aerial mycelium | ISP 6 | |
69335 | yes | Aerial mycelium | ISP 7 | |
69335 | yes | Aerial mycelium | Traffic white (9016) | suter with tyrosine |
69335 | no | Aerial mycelium | suter without tyrosine |
pigmentation
@ref | production | name |
---|---|---|
30450 | yes | |
69335 | no | soluble pigment |
multimedia
@ref | multimedia content | caption | intellectual property rights |
---|---|---|---|
18063 | https://www.dsmz.de/microorganisms/photos/DSM_42061.jpg | Medium 65 28°C | © Leibniz-Institut DSMZ |
69335 | DSM_42061_image3.jpeg | Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7) | Helmholtz-Zentrum für Infektionsforschung GmbH |
69335 | DSM_42061_image4.jpeg | Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7) | Helmholtz-Zentrum für Infektionsforschung GmbH |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
18063 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
18063 | STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) | yes | https://mediadive.dsmz.de/medium/252 | Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
18063 | positive | growth | 28 | mesophilic |
30450 | positive | growth | 10-37 | |
67770 | positive | growth | 28 | mesophilic |
culture pH
- @ref: 30450
- ability: positive
- type: growth
- pH: 05-09
- PH range: alkaliphile
Physiology and metabolism
oxygen tolerance
- @ref: 30450
- oxygen tolerance: aerobe
spore formation
- @ref: 30450
- spore formation: yes
halophily
- @ref: 69335
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-2.5 %
observation
- @ref: 67770
- observation: quinones: MK-9 (H6, MK-9(H8)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
69335 | 22599 | arabinose | + | growth |
30450 | 18403 | L-arabitol | + | carbon source |
30450 | 17306 | maltose | + | carbon source |
30450 | 28053 | melibiose | + | carbon source |
30450 | 30911 | sorbitol | + | carbon source |
30450 | 4853 | esculin | + | hydrolysis |
69335 | 62968 | cellulose | - | growth |
69335 | 28757 | fructose | +/- | growth |
69335 | 17234 | glucose | + | growth |
69335 | 17268 | inositol | + | growth |
69335 | 37684 | mannose | + | growth |
69335 | 16634 | raffinose | + | growth |
69335 | 26546 | rhamnose | + | growth |
69335 | 17992 | sucrose | +/- | growth |
69335 | 18222 | xylose | + | growth |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | - | hydrolysis |
68379 | 16199 | urea | - | hydrolysis |
68379 | 5291 | gelatin | + | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
30450 | gelatinase | + | |
68379 | gelatinase | + | |
68379 | urease | - | 3.5.1.5 |
68379 | beta-glucosidase | - | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | + | 3.2.1.20 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | + | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | + | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69335 | - | - | - | + | - | +/- | + | - | - | - | + | - | - | - | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69335 | + | +/- | +/- | - | + | + | + | - | - | + | + | - | + | - | + | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
18063 | soil of a hay meadow | Northumberland, Cockle Park Experimental Farm | United Kingdom | GBR | Europe |
67770 | Soil of a hay meadow at Cockle Park Experimental Farm | Ulgham, Morpeth, Northumberland | United Kingdom | GBR | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Agriculture | #Meadow |
#Environmental | #Terrestrial | #Soil |
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
Safety information
risk assessment
- @ref: 18063
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 18063
- description: Streptomyces pratens partial 16S rRNA gene, type strain BK138T
- accession: FR692098
- length: 1458
- database: ena
- NCBI tax ID: 887456
GC content
@ref | GC-content | method |
---|---|---|
18063 | 73.2 | thermal denaturation, midpoint method (Tm) |
30450 | 73.2 |
External links
@ref: 18063
culture collection no.: DSM 42061, CGMCC 4.5800, KACC 20904, NRRL B-59131, JCM 19679
straininfo link
- @ref: 85531
- straininfo: 401949
literature
- topic: Phylogeny
- Pubmed-ID: 21984681
- title: Streptomyces herbaceus sp. nov., Streptomyces incanus sp. nov. and Streptomyces pratens sp. nov., isolated from the soil of a hay meadow.
- authors: Kim BY, Rong X, Zucchi TD, Bonda ANV, Huang Y, Goodfellow M
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.036384-0
- year: 2011
- mesh: Bacterial Typing Techniques, DNA, Bacterial/genetics, Genotype, Microscopy, Electron, Scanning, Molecular Sequence Data, Phenotype, *Phylogeny, Poaceae, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil, *Soil Microbiology, Species Specificity, Spores, Bacterial/ultrastructure, Streptomyces/*classification/genetics/isolation & purification
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
18063 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42061) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-42061 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
30450 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26785 | 28776041 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68379 | Automatically annotated from API Coryne | |||||
68382 | Automatically annotated from API zym | |||||
69335 | Wink, J. | https://cdn.dsmz.de/wink/DSM%2042061.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
85531 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID401949.1 | StrainInfo: A central database for resolving microbial strain identifiers |