Strain identifier

BacDive ID: 16498

Type strain: Yes

Species: Streptomyces pratens

Strain Designation: BK138

Strain history: CGMCC 4.5800 <-- B.-Y. Kim et al. BK138.

NCBI tax ID(s): 887456 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 18063

BacDive-ID: 16498

DSM-Number: 42061

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Streptomyces pratens BK138 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil of a hay meadow.

NCBI tax id

  • NCBI tax id: 887456
  • Matching level: species

strain history

@refhistory
18063<- S. W. Kwon, KACC; KACC 20904
67770CGMCC 4.5800 <-- B.-Y. Kim et al. BK138.

doi: 10.13145/bacdive16498.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces pratens
  • full scientific name: Streptomyces pratens Kim et al. 2012

@ref: 18063

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces pratens

full scientific name: Streptomyces pratens Kim et al. 2012

strain designation: BK138

type strain: yes

Morphology

cell morphology

  • @ref: 30450
  • gram stain: positive

colony morphology

@refcolony colormedium used
69335Ivory (1014)ISP 2
69335Ivory (1014)ISP 5
69335Light ivory (1015), oyster white (1013)ISP 7
69335Ochre yellow (1024)ISP 6
69335Oyster white (1013)ISP 4
69335Sand yellow (1002)suter without tyrosine

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69335yesAerial myceliumTraffic white (9016)ISP 2
69335yesAerial myceliumYellow grey (7034), traffic white (9016)ISP 3
69335yesAerial myceliumGrey white (9002)ISP 4
69335yesAerial myceliumISP 5
69335yesAerial myceliumISP 6
69335yesAerial myceliumISP 7
69335yesAerial myceliumTraffic white (9016)suter with tyrosine
69335noAerial myceliumsuter without tyrosine

pigmentation

@refproductionname
30450yes
69335nosoluble pigment

multimedia

@refmultimedia contentcaptionintellectual property rights
18063https://www.dsmz.de/microorganisms/photos/DSM_42061.jpgMedium 65 28°C© Leibniz-Institut DSMZ
69335DSM_42061_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69335DSM_42061_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18063GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18063STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
18063positivegrowth28mesophilic
30450positivegrowth10-37
67770positivegrowth28mesophilic

culture pH

  • @ref: 30450
  • ability: positive
  • type: growth
  • pH: 05-09
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 30450
  • oxygen tolerance: aerobe

spore formation

  • @ref: 30450
  • spore formation: yes

halophily

  • @ref: 69335
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-2.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9 (H6, MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6933522599arabinose+growth
3045018403L-arabitol+carbon source
3045017306maltose+carbon source
3045028053melibiose+carbon source
3045030911sorbitol+carbon source
304504853esculin+hydrolysis
6933562968cellulose-growth
6933528757fructose+/-growth
6933517234glucose+growth
6933517268inositol+growth
6933537684mannose+growth
6933516634raffinose+growth
6933526546rhamnose+growth
6933517992sucrose+/-growth
6933518222xylose+growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
30450gelatinase+
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69335---+-+/-+---+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69335++/-+/--+++--++-+-+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
18063soil of a hay meadowNorthumberland, Cockle Park Experimental FarmUnited KingdomGBREurope
67770Soil of a hay meadow at Cockle Park Experimental FarmUlgham, Morpeth, NorthumberlandUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Meadow
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)

Safety information

risk assessment

  • @ref: 18063
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18063
  • description: Streptomyces pratens partial 16S rRNA gene, type strain BK138T
  • accession: FR692098
  • length: 1458
  • database: ena
  • NCBI tax ID: 887456

GC content

@refGC-contentmethod
1806373.2thermal denaturation, midpoint method (Tm)
3045073.2

External links

@ref: 18063

culture collection no.: DSM 42061, CGMCC 4.5800, KACC 20904, NRRL B-59131, JCM 19679

straininfo link

  • @ref: 85531
  • straininfo: 401949

literature

  • topic: Phylogeny
  • Pubmed-ID: 21984681
  • title: Streptomyces herbaceus sp. nov., Streptomyces incanus sp. nov. and Streptomyces pratens sp. nov., isolated from the soil of a hay meadow.
  • authors: Kim BY, Rong X, Zucchi TD, Bonda ANV, Huang Y, Goodfellow M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.036384-0
  • year: 2011
  • mesh: Bacterial Typing Techniques, DNA, Bacterial/genetics, Genotype, Microscopy, Electron, Scanning, Molecular Sequence Data, Phenotype, *Phylogeny, Poaceae, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil, *Soil Microbiology, Species Specificity, Spores, Bacterial/ultrastructure, Streptomyces/*classification/genetics/isolation & purification
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
18063Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42061)https://www.dsmz.de/collection/catalogue/details/culture/DSM-42061
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30450Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2678528776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69335Wink, J.https://cdn.dsmz.de/wink/DSM%2042061.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
85531Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID401949.1StrainInfo: A central database for resolving microbial strain identifiers