Strain identifier

BacDive ID: 16480

Type strain: Yes

Species: Streptomyces youssoufiensis

Strain Designation: X4

Strain history: DSM 41920 <-- Y. Ouhdouch X4 <-- H. Hamdali.

NCBI tax ID(s): 654447 (species)

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General

@ref: 10660

BacDive-ID: 16480

DSM-Number: 41920

keywords: 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces youssoufiensis X4 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from Moroccan rock phosphate mine.

NCBI tax id

  • NCBI tax id: 654447
  • Matching level: species

strain history

@refhistory
10660<- Y. Ouhdouch, Univ. Cadi Ayyad, Marrakech, Morocco; X4 <- H. Hamdali
67770DSM 41920 <-- Y. Ouhdouch X4 <-- H. Hamdali.

doi: 10.13145/bacdive16480.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces youssoufiensis
  • full scientific name: Streptomyces youssoufiensis Hamdali et al. 2011

@ref: 10660

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces youssoufiensis

full scientific name: Streptomyces youssoufiensis Hamdali et al. 2011

strain designation: X4

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
21385Broom yellow (1032)10-14 daysISP 2
21385Ivory (1014)10-14 daysISP 3
2138510-14 daysISP 4
21385Broom yellow (1032)10-14 daysISP 5
21385Dahlia yellow (1033)10-14 daysISP 7
21385Golden yellow (1004)10-14 daysSuter with tyrosine
21385Saffron yellow (1017)10-14 daysSuter without tyrosine

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
21385yesAerial MyceliumTraffic white (9016)ISP 2
21385noISP 3
21385yesAerial MyceliumTraffic yellow (1023)ISP 4
21385yesAerial MyceliumPure white (9010)ISP 5
21385yesAerial MyceliumCreamISP 7
21385yesAerial MyceliumCream (9001)Suter with tyrosine
21385yesAerial MyceliumSignal yellow (9003)Suter without tyrosine

pigmentation

  • @ref: 29864
  • production: no

multimedia

  • @ref: 10660
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_41920.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10660GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
21385ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
21385ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
21385ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
21385ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
21385ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
21385Suter with tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
21385Suter without tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
10660positivegrowth28mesophilic
29864positivegrowth28-42
29864positiveoptimum35mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
29864positivegrowth05-10alkaliphile
29864positiveoptimum7.5

Physiology and metabolism

spore formation

  • @ref: 29864
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
21385NaClpositivemaximum2.5 %
29864NaClpositivegrowth<7 %

observation

  • @ref: 29864
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2138517234glucose+
2138522599arabinose-
2138517992sucrose-
2138518222xylose-
2138517268myo-inositol-
2138537684mannose+
2138528757fructose-
2138526546rhamnose-
2138516634raffinose+
2138562968cellulose-
2986417057cellobiose+carbon source
2986428757fructose+carbon source
2986417234glucose+carbon source
2986417716lactose+carbon source
2986417306maltose+carbon source
2986429864mannitol+carbon source
2986437684mannose+carbon source
2986417992sucrose+carbon source
2986417632nitrate+reduction
6837917632nitrate-reduction
683794853esculin+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
21385--++----++/-+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
21385+++-+++/-++++-+-+--+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
10660Moroccan rock phosphate mineYoussoufiaMoroccoMARAfrica
67770Phosphate mine in Youssoufia100 km north of MarrakeshMoroccoMARAfrica

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Other
  • Cat3: #Mine

Safety information

risk assessment

  • @ref: 10660
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 10660
  • description: Streptomyces youssoufiensis 16S rRNA gene, type strain DSM 41920
  • accession: FN421338
  • length: 1518
  • database: ena
  • NCBI tax ID: 654447

External links

@ref: 10660

culture collection no.: DSM 41920, CCMM B709, JCM 18307

straininfo link

  • @ref: 85515
  • straininfo: 381918

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20525811Streptomyces youssoufiensis sp. nov., isolated from a Moroccan phosphate mine.Hamdali H, Virolle MJ, von Jan M, Sproer C, Klenk HP, Ouhdouch YInt J Syst Evol Microbiol10.1099/ijs.0.023036-02010DNA, Bacterial/genetics, Fatty Acids/metabolism, Mining, Molecular Sequence Data, Morocco, Phosphates/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification/metabolismMetabolism
Phylogeny25030519Streptomyces zagrosensis sp. nov., isolated from soil.Mohammadipanah F, Hamedi J, Sproer C, Rohde M, Montero-Calasanz MDC, Klenk HPInt J Syst Evol Microbiol10.1099/ijs.0.064527-02014Bacterial Typing Techniques, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Iran, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purificationGenetics
Phylogeny36512155Diversity of actinomycete and their metabolites isolated from Howz Soltan Lake, Iran.Salehghamari E, Moradi M, Sardabi M, Etesami SA, Hassani GH, Hosseini M, Taheri F, Eshrati Z, Tahmaseb M, Irian S, Amoozegar MAArch Microbiol10.1007/s00203-022-03364-02022Humans, *Actinobacteria, RNA, Ribosomal, 16S/genetics/metabolism, Lakes/microbiology, Phylogeny, Iran, ActinomycesPathogenicity

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
10660Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41920)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41920
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
21385Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41920.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
29864Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2623828776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
85515Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID381918.1StrainInfo: A central database for resolving microbial strain identifiers