Strain identifier
BacDive ID: 16472
Type strain:
Species: Streptomyces haliclonae
Strain Designation: Sp080513SC-31
Strain history: <- T. Tamura, NBRC; NBRC 105049 <- S. T. Khan; Sp080513SC-31
NCBI tax ID(s): 582855 (species)
General
@ref: 16407
BacDive-ID: 16472
DSM-Number: 41970
keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped
description: Streptomyces haliclonae Sp080513SC-31 is an aerobe, spore-forming, mesophilic bacterium that was isolated from marine sponge Haliclona sp..
NCBI tax id
- NCBI tax id: 582855
- Matching level: species
strain history
- @ref: 16407
- history: <- T. Tamura, NBRC; NBRC 105049 <- S. T. Khan; Sp080513SC-31
doi: 10.13145/bacdive16472.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces haliclonae
- full scientific name: Streptomyces haliclonae Khan et al. 2010
@ref: 16407
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces haliclonae
full scientific name: Streptomyces haliclonae Khan et al. 2010
strain designation: Sp080513SC-31
type strain: yes
Morphology
cell morphology
- @ref: 29742
- gram stain: positive
- cell shape: rod-shaped
- motility: yes
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
18733 | Beige (1001) | 10-14 days | ISP 3 |
18733 | Beige (1001) | 10-14 days | ISP 4 |
18733 | Beige (1001) | 10-14 days | ISP 5 |
18733 | Beige (1001) | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | medium name |
---|---|---|
18733 | no | ISP 2 |
18733 | no | ISP 3 |
18733 | no | ISP 4 |
18733 | no | ISP 5 |
18733 | no | ISP 6 |
18733 | no | ISP 7 |
pigmentation
- @ref: 29742
- production: yes
multimedia
- @ref: 16407
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_41970.jpg
- caption: Medium 252 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
16407 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
18733 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
18733 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
18733 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
18733 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
16407 | positive | growth | 28 | mesophilic |
18733 | positive | optimum | 28 | mesophilic |
29742 | positive | growth | 15-37 | |
29742 | positive | optimum | 25-30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
29742 | positive | growth | 06-09 | alkaliphile |
29742 | positive | optimum | 06-08 |
Physiology and metabolism
oxygen tolerance
- @ref: 29742
- oxygen tolerance: aerobe
spore formation
- @ref: 29742
- spore formation: yes
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
29742 | NaCl | positive | growth | 01-10 % |
29742 | NaCl | positive | optimum | 02-07 % |
observation
- @ref: 29742
- observation: aggregates in chains
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
29742 | 28757 | fructose | + | carbon source |
29742 | 17234 | glucose | + | carbon source |
29742 | 29864 | mannitol | + | carbon source |
29742 | 16634 | raffinose | + | carbon source |
29742 | 30911 | sorbitol | + | carbon source |
29742 | 17992 | sucrose | + | carbon source |
enzymes
@ref | value | activity | ec |
---|---|---|---|
29742 | acid phosphatase | + | 3.1.3.2 |
29742 | alkaline phosphatase | + | 3.1.3.1 |
29742 | alpha-galactosidase | + | 3.2.1.22 |
29742 | catalase | + | 1.11.1.6 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
18733 | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 16407
- sample type: marine sponge Haliclona sp.
- host species: Haliclona
- geographic location: Chiba prefecture, pacific coastline of Tateyama City
- country: Japan
- origin.country: JPN
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Marine |
#Host | #Invertebrates (Other) | #Porifera (Sponges) |
Safety information
risk assessment
- @ref: 16407
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16407
- description: Streptomyces haliclonae gene for 16S rRNA, partial sequence
- accession: AB473556
- length: 1459
- database: ena
- NCBI tax ID: 582855
GC content
@ref | GC-content | method |
---|---|---|
16407 | 73 | high performance liquid chromatography (HPLC) |
29742 | 73 |
External links
@ref: 16407
culture collection no.: DSM 41970, NBRC 105049
straininfo link
- @ref: 85508
- straininfo: 369698
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 20061489 | Streptomyces tateyamensis sp. nov., Streptomyces marinus sp. nov. and Streptomyces haliclonae sp. nov., isolated from the marine sponge Haliclona sp. | Khan ST, Tamura T, Takagi M, Shin-Ya K | Int J Syst Evol Microbiol | 10.1099/ijs.0.019869-0 | 2010 | Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Haliclona/*microbiology, Japan, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
Phylogeny | 26755354 | Streptomyces daqingensis sp. nov., isolated from saline-alkaline soil. | Pan T, He H, Li C, Zhao J, Zhang Y, Li J, Wang X, Liu C, Zhang J, Xiang W | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000887 | 2016 | ||
Phylogeny | 33661469 | Genome mining and description of Streptomyces albidus sp. nov., an endophytic actinobacterium with antibacterial potential. | Kaewkla O, Franco CMM | Antonie Van Leeuwenhoek | 10.1007/s10482-021-01539-1 | 2021 | Anti-Bacterial Agents/pharmacology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid, Fatty Acids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Streptomyces/genetics | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
16407 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41970) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-41970 | |||
18733 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM41970.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
29742 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26124 | 28776041 | |
68382 | Automatically annotated from API zym | |||||
85508 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID369698.1 | StrainInfo: A central database for resolving microbial strain identifiers |