Strain identifier

BacDive ID: 16472

Type strain: Yes

Species: Streptomyces haliclonae

Strain Designation: Sp080513SC-31

Strain history: <- T. Tamura, NBRC; NBRC 105049 <- S. T. Khan; Sp080513SC-31

NCBI tax ID(s): 582855 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 16407

BacDive-ID: 16472

DSM-Number: 41970

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Streptomyces haliclonae Sp080513SC-31 is an aerobe, spore-forming, mesophilic bacterium that was isolated from marine sponge Haliclona sp..

NCBI tax id

  • NCBI tax id: 582855
  • Matching level: species

strain history

  • @ref: 16407
  • history: <- T. Tamura, NBRC; NBRC 105049 <- S. T. Khan; Sp080513SC-31

doi: 10.13145/bacdive16472.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces haliclonae
  • full scientific name: Streptomyces haliclonae Khan et al. 2010

@ref: 16407

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces haliclonae

full scientific name: Streptomyces haliclonae Khan et al. 2010

strain designation: Sp080513SC-31

type strain: yes

Morphology

cell morphology

  • @ref: 29742
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

@refcolony colorincubation periodmedium used
18733Beige (1001)10-14 daysISP 3
18733Beige (1001)10-14 daysISP 4
18733Beige (1001)10-14 daysISP 5
18733Beige (1001)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18733noISP 2
18733noISP 3
18733noISP 4
18733noISP 5
18733noISP 6
18733noISP 7

pigmentation

  • @ref: 29742
  • production: yes

multimedia

  • @ref: 16407
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_41970.jpg
  • caption: Medium 252 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16407GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18733ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18733ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18733ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18733ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
16407positivegrowth28mesophilic
18733positiveoptimum28mesophilic
29742positivegrowth15-37
29742positiveoptimum25-30mesophilic

culture pH

@refabilitytypepHPH range
29742positivegrowth06-09alkaliphile
29742positiveoptimum06-08

Physiology and metabolism

oxygen tolerance

  • @ref: 29742
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29742
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
29742NaClpositivegrowth01-10 %
29742NaClpositiveoptimum02-07 %

observation

  • @ref: 29742
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2974228757fructose+carbon source
2974217234glucose+carbon source
2974229864mannitol+carbon source
2974216634raffinose+carbon source
2974230911sorbitol+carbon source
2974217992sucrose+carbon source

enzymes

@refvalueactivityec
29742acid phosphatase+3.1.3.2
29742alkaline phosphatase+3.1.3.1
29742alpha-galactosidase+3.2.1.22
29742catalase+1.11.1.6
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18733----------+--------

Isolation, sampling and environmental information

isolation

  • @ref: 16407
  • sample type: marine sponge Haliclona sp.
  • host species: Haliclona
  • geographic location: Chiba prefecture, pacific coastline of Tateyama City
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Host#Invertebrates (Other)#Porifera (Sponges)

Safety information

risk assessment

  • @ref: 16407
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16407
  • description: Streptomyces haliclonae gene for 16S rRNA, partial sequence
  • accession: AB473556
  • length: 1459
  • database: ena
  • NCBI tax ID: 582855

GC content

@refGC-contentmethod
1640773high performance liquid chromatography (HPLC)
2974273

External links

@ref: 16407

culture collection no.: DSM 41970, NBRC 105049

straininfo link

  • @ref: 85508
  • straininfo: 369698

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20061489Streptomyces tateyamensis sp. nov., Streptomyces marinus sp. nov. and Streptomyces haliclonae sp. nov., isolated from the marine sponge Haliclona sp.Khan ST, Tamura T, Takagi M, Shin-Ya KInt J Syst Evol Microbiol10.1099/ijs.0.019869-02010Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Haliclona/*microbiology, Japan, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny26755354Streptomyces daqingensis sp. nov., isolated from saline-alkaline soil.Pan T, He H, Li C, Zhao J, Zhang Y, Li J, Wang X, Liu C, Zhang J, Xiang WInt J Syst Evol Microbiol10.1099/ijsem.0.0008872016
Phylogeny33661469Genome mining and description of Streptomyces albidus sp. nov., an endophytic actinobacterium with antibacterial potential.Kaewkla O, Franco CMMAntonie Van Leeuwenhoek10.1007/s10482-021-01539-12021Anti-Bacterial Agents/pharmacology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid, Fatty Acids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Streptomyces/geneticsTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16407Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41970)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41970
18733Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41970.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29742Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2612428776041
68382Automatically annotated from API zym
85508Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID369698.1StrainInfo: A central database for resolving microbial strain identifiers