Strain identifier

BacDive ID: 16470

Type strain: Yes

Species: Streptomyces wellingtoniae

Strain Designation: FD 11369

Strain history: <- J.B. Routien, Chas. Pfizer & Co., FD 11369 <- NRRL, [ETH 15973]

NCBI tax ID(s): 476555 (species)

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General

@ref: 9584

BacDive-ID: 16470

DSM-Number: 40632

keywords: 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces wellingtoniae FD 11369 is a spore-forming, mesophilic bacterium that builds an aerial mycelium.

NCBI tax id

  • NCBI tax id: 476555
  • Matching level: species

strain history

  • @ref: 9584
  • history: <- J.B. Routien, Chas. Pfizer & Co., FD 11369 <- NRRL, [ETH 15973]

doi: 10.13145/bacdive16470.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces wellingtoniae
  • full scientific name: Streptomyces wellingtoniae Kumar and Goodfellow 2010

@ref: 9584

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces wellingtoniae

full scientific name: Streptomyces wellingtoniae Kumar and Goodfellow 2010

strain designation: FD 11369

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
18663Jet black (9005)10-14 daysISP 2
18663Purple violet (4007)10-14 daysISP 3
18663Grey brown (8019)10-14 daysISP 4
18663Purple violet (4007)10-14 daysISP 5
18663Brown beige (1011)10-14 daysISP 6
18663Terra brown (8028)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
18663yesAerial MyceliumTraffic grey A (7042)ISP 2
18663yesAerial MyceliumTraffic grey A (7042)ISP 3
18663yesAerial MyceliumTraffic grey A (7042)ISP 4
18663noISP 5
18663noISP 6
18663noISP 7

pigmentation

  • @ref: 29391
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9584GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
9584ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf
18663ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18663ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18663ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18663ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18663ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18663ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
9584positivegrowth28mesophilic
18663positiveoptimum28mesophilic

culture pH

  • @ref: 29391
  • ability: positive
  • type: growth
  • pH: 05-10
  • PH range: alkaliphile

Physiology and metabolism

spore formation

  • @ref: 29391
  • spore formation: yes

compound production

@refcompound
9584hygroscopin A
9584hygroscopin B
9584hygroscopin C
9584hygroscopin A, B, C

halophily

  • @ref: 29391
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 10-13 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1866317234glucose+
1866322599arabinose+
1866317992sucrose+/-
1866318222xylose-
1866317268myo-inositol+
1866329864mannitol+
1866328757fructose+
1866326546rhamnose+
1866316634raffinose+
1866362968cellulose-
2939122599arabinose+carbon source
2939118403L-arabitol+carbon source
2939117057cellobiose+carbon source
2939123652dextrin+carbon source
2939128757fructose+carbon source
2939128260galactose+carbon source
2939129987glutamate+carbon source
2939128087glycogen+carbon source
2939117716lactose+carbon source
2939125017leucine+carbon source
2939117268myo-inositol+carbon source
2939126271proline+carbon source
2939116634raffinose+carbon source
2939126546rhamnose+carbon source
2939133942ribose+carbon source
2939118222xylose+carbon source

enzymes

@refvalueactivityec
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18663++++++---++-+-++++-

Safety information

risk assessment

  • @ref: 9584
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 9584
  • description: Streptomyces wellingtoniae strain DSM 40632 16S ribosomal RNA gene, partial sequence
  • accession: EU170124
  • length: 1449
  • database: ena
  • NCBI tax ID: 476555

GC content

  • @ref: 9584
  • GC-content: 72

External links

@ref: 9584

culture collection no.: DSM 40632, ETH 15973, NRRL B-1503

straininfo link

  • @ref: 85506
  • straininfo: 297027

literature

  • topic: Phylogeny
  • Pubmed-ID: 19656929
  • title: Reclassification of Streptomyces hygroscopicus strains as Streptomyces aldersoniae sp. nov., Streptomyces angustmyceticus sp. nov., comb. nov., Streptomyces ascomycinicus sp. nov., Streptomyces decoyicus sp. nov., comb. nov., Streptomyces milbemycinicus sp. nov. and Streptomyces wellingtoniae sp. nov.
  • authors: Kumar Y, Goodfellow M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.012161-0
  • year: 2009
  • mesh: Bacterial Typing Techniques, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/analysis, Genes, rRNA, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Soil Microbiology, Species Specificity, Streptomyces/*classification/genetics/metabolism/physiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
9584Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40632)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40632
18663Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40632.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29391Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2579828776041
68382Automatically annotated from API zym
85506Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297027.1StrainInfo: A central database for resolving microbial strain identifiers