Strain identifier

BacDive ID: 16458

Type strain: Yes

Species: Streptomyces axinellae

Strain Designation: Pol001, P01001

Strain history: CIP <- 2008, M. Schenermayer, Würzburg Univ., Würzburg, Germany <- S.M. Pimentel-Elardo, Würzburg Univ., Würzburg, Germany: strain P01001

NCBI tax ID(s): 552788 (species)

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General

@ref: 15800

BacDive-ID: 16458

DSM-Number: 41948

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Streptomyces axinellae Pol001 is an aerobe, spore-forming, mesophilic bacterium that was isolated from Mediterranean sponge Axinella polypoides.

NCBI tax id

  • NCBI tax id: 552788
  • Matching level: species

strain history

@refhistory
15800<- S. Pimentel-Elardo; Pol001 <- M. Scheuermayer
67770CIP 109838 <-- M. Schenermayer <-- S. M. Pimentel-Elardo Pol001.
116685CIP <- 2008, M. Schenermayer, Würzburg Univ., Würzburg, Germany <- S.M. Pimentel-Elardo, Würzburg Univ., Würzburg, Germany: strain P01001

doi: 10.13145/bacdive16458.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces axinellae
  • full scientific name: Streptomyces axinellae Pimentel-Elardo et al. 2009

@ref: 15800

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces axinellae

full scientific name: Streptomyces axinellae Pimentel-Elardo et al. 2009

strain designation: Pol001, P01001

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
29170positive
116685positiverod-shapedno

pigmentation

  • @ref: 29170
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15800GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
37856MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
15800STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
15800ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
116685CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
15800positivegrowth28mesophilic
29170positivegrowth20-37
29170positiveoptimum30mesophilic
37856positivegrowth30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29170aerobe
116685obligate aerobe

spore formation

  • @ref: 29170
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
29170NaClpositivegrowth0-7.5 %
29170NaClpositiveoptimum1.25 %

observation

@refobservation
29170aggregates in chains
67770quinones: MK-9(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2917021217L-alaninamide+carbon source
2917016449alanine+carbon source
2917018403L-arabitol+carbon source
2917017057cellobiose+carbon source
2917023652dextrin+carbon source
2917028757fructose+carbon source
2917028260galactose+carbon source
291705291gelatin+carbon source
2917024265gluconate+carbon source
291705417glucosamine+carbon source
2917017234glucose+carbon source
2917029987glutamate+carbon source
2917017754glycerol+carbon source
2917017716lactose+carbon source
2917029864mannitol+carbon source
2917037684mannose+carbon source
2917017272propionate+carbon source
2917026546rhamnose+carbon source
2917033942ribose+carbon source
2917017822serine+carbon source
2917053423tween 40+carbon source
2917053426tween 80+carbon source
2917018222xylose+carbon source
11668517632nitrate+reduction
11668516301nitrite-reduction

metabolite production

  • @ref: 116685
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
116685oxidase-
116685catalase+1.11.1.6
116685urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116685-+++-+----++-+-++++-

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentisolation date
15800Mediterranean sponge Axinella polypoidesAxinella polypoidesBanyuls-sur-MerFranceFRAEurope
67770Mediterranean sponge Axinella polypoidesAxinella polypoidesBanyuls-sur-MerFranceFRAEurope
116685Animal, Marine sponge, Axinella polypoidesBanuyls sur merFranceFRAEurope2003

isolation source categories

  • Cat1: #Host
  • Cat2: #Invertebrates (Other)
  • Cat3: #Porifera (Sponges)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
158001Risk group (German classification)
1166851Risk group (French classification)

Sequence information

16S sequences

  • @ref: 15800
  • description: Streptomyces axinellae strain Pol001 16S ribosomal RNA gene, partial sequence
  • accession: EU683612
  • length: 1422
  • database: ena
  • NCBI tax ID: 552788

GC content

@refGC-contentmethod
1580071.0high performance liquid chromatography (HPLC)
2917071
6777071high performance liquid chromatography (HPLC)

External links

@ref: 15800

culture collection no.: DSM 41948, CIP 109838, JCM 16373

straininfo link

  • @ref: 85494
  • straininfo: 362857

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19502329Streptomyces axinellae sp. nov., isolated from the Mediterranean sponge Axinella polypoides (Porifera).Pimentel-Elardo SM, Scheuermayer M, Kozytska S, Hentschel UInt J Syst Evol Microbiol10.1099/ijs.0.007856-02009Animals, Axinella/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, France, Genotype, Mediterranean Sea, Molecular Sequence Data, Phenotype, Phylogeny, Polymerase Chain Reaction/methods, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Streptomyces/*classification/genetics/*isolation & purification/physiologyGenetics
Enzymology22072992New tetromycin derivatives with anti-trypanosomal and protease inhibitory activities.Pimentel-Elardo SM, Buback V, Gulder TA, Bugni TS, Reppart J, Bringmann G, Ireland CM, Schirmeister T, Hentschel UMar Drugs10.3390/md91016822011Animals, Antiprotozoal Agents/isolation & purification/pharmacology, Axinella/microbiology, Cathepsin B/antagonists & inhibitors, Cathepsin L/antagonists & inhibitors, Coronavirus 3C Proteases, Cysteine Endopeptidases/drug effects, Heterocyclic Compounds, 4 or More Rings/*isolation & purification/pharmacology, Leishmania major/drug effects, Magnetic Resonance Spectroscopy, Protease Inhibitors/*isolation & purification/pharmacology, SARS Virus/drug effects, Streptomyces/chemistry, Trypanocidal Agents/*isolation & purification/pharmacology, Trypanosoma brucei brucei/drug effects, Viral Proteins/antagonists & inhibitorsPhylogeny

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15800Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41948)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41948
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29170Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2559428776041
37856Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7626
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
85494Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID362857.1StrainInfo: A central database for resolving microbial strain identifiers
116685Curators of the CIPCollection of Institut Pasteur (CIP 109838)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109838