Archive BacDiveID:10.13145/bacdive16439.20230509.8.1

Strain identifier

BacDive ID: 16439

Type strain:

Species: Streptomyces scopuliridis

Strain history: <- J. B. Olson; RB72 <- M. H. Farris

NCBI tax ID(s): 1440053 (strain), 452529 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 10449

BacDive-ID: 16439

DSM-Number: 41917

keywords: 16S sequence, Bacteria, mesophilic

description: Streptomyces scopuliridis DSM 41917 is a mesophilic bacterium that builds an aerial mycelium and was isolated from woodland bluff soil.

NCBI tax id

NCBI tax id	Matching level
452529	species
1440053	strain

strain history

@ref: 10449
history: <- J. B. Olson; RB72 <- M. H. Farris

doi: 10.13145/bacdive16439.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/actinomycetota
domain: Bacteria
phylum: Actinomycetota
class: Actinomycetes
order: Kitasatosporales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces scopuliridis
full scientific name: Streptomyces scopuliridis Farris et al. 2011

@ref: 10449

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces scopuliridis

full scientific name: Streptomyces scopuliridis Farris et al. 2011

type strain: yes

Morphology

colony morphology

@ref	colony color	incubation period	medium used
21418	Light ivory (1015)	10-14 days	ISP 2
21418		10-14 days	ISP 3
21418	Colorless	10-14 days	ISP 4
21418		10-14 days	ISP 5
21418	Colorless	10-14 days	ISP 6
21418		10-14 days	ISP 7
21418	Light ivory (1015)	10-14 days	suter with tyrosine
21418	Ivory (1014)	10-14 days	Suter without tyrosine

multicellular morphology

@ref	forms multicellular complex	complex name	complex color	medium name
21418	yes	Aerial Mycelium	Pure white (9010)	ISP 2
21418	yes	Aerial Mycelium	Traffic white (9016)	ISP 3
21418	yes	Aerial Mycelium	Traffic white (9016)	ISP 4
21418	yes	Aerial Mycelium	Signal white (9003)	ISP 5
21418	no			ISP 6
21418	yes	Aerial Mycelium	Pure white (9010)/ signal white (9003)	ISP 7
21418	yes	Aerial Mycelium	Traffic white (9016)	Suter with tyrosine
21418	no			Suter without tyrosine

multimedia

@ref	multimedia content	intellectual property rights	caption
10449	https://www.dsmz.de/microorganisms/photos/DSM_41917-1.jpg	© Leibniz-Institut DSMZ
10449	https://www.dsmz.de/microorganisms/photos/DSM_41917.jpg	© Leibniz-Institut DSMZ	Medium 65 28°C

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
10449	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	yes	https://mediadive.dsmz.de/medium/65	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
21418	ISP 2	yes		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
21418	ISP 3	yes		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
21418	ISP 4	yes		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
21418	ISP 5	yes		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
21418	ISP 6	yes		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
21418	ISP 7	yes		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
21418	suter with tyrosine	yes		Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
21418	Suter without tyrosine	yes		Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
10449	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)	yes	https://mediadive.dsmz.de/medium/84	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
10449	STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)	yes	https://mediadive.dsmz.de/medium/252	Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water

culture temp

@ref: 10449
growth: positive
type: growth
temperature: 28
range: mesophilic

Physiology and metabolism

compound production

@ref: 10449
compound: bacteriocin

halophily

@ref: 21418
salt: NaCl
growth: positive
tested relation: maximum
concentration: 7.5 %

metabolite utilization

@ref	Chebi-ID	metabolite	utilization activity	kind of utilization tested
21418	17234	glucose	+
21418	22599	arabinose	+
21418	17992	sucrose	+
21418	18222	xylose	+
21418	17268	myo-inositol	+
21418	37684	mannose	-
21418	28757	fructose	+
21418	26546	rhamnose	-
21418	16634	raffinose	+
21418	62968	cellulose	-
68379	17632	nitrate	-	reduction
68379	4853	esculin	-	hydrolysis
68379	16199	urea	+	hydrolysis
68379	5291	gelatin	+	hydrolysis
68379	17634	D-glucose	-	fermentation
68379	16988	D-ribose	-	fermentation
68379	65327	D-xylose	-	fermentation
68379	16899	D-mannitol	-	fermentation
68379	17306	maltose	-	fermentation
68379	17716	lactose	-	fermentation
68379	17992	sucrose	-	fermentation
68379	28087	glycogen	-	fermentation

enzymes

@ref	value	activity	ec
68379	gelatinase	+
68379	urease	+	3.5.1.5
68379	beta-glucosidase	-	3.2.1.21
68379	alpha-glucosidase	+	3.2.1.20
68379	beta-galactosidase	-	3.2.1.23
68379	beta-glucuronidase	-	3.2.1.31
68379	alkaline phosphatase	+	3.1.3.1
68379	pyrrolidonyl arylamidase	-	3.4.19.3
68379	pyrazinamidase	-	3.5.1.B15
68382	alkaline phosphatase	+	3.1.3.1
68382	esterase (C 4)	+
68382	lipase (C 14)	-
68382	leucine arylamidase	+	3.4.11.1
68382	valine arylamidase	+
68382	trypsin	+	3.4.21.4
68382	alpha-chymotrypsin	+	3.4.21.1
68382	acid phosphatase	+	3.1.3.2
68382	naphthol-AS-BI-phosphohydrolase	+
68382	alpha-galactosidase	-	3.2.1.22
68382	beta-galactosidase	-	3.2.1.23
68382	beta-glucuronidase	-	3.2.1.31
68382	alpha-glucosidase	+	3.2.1.20
68382	beta-glucosidase	+	3.2.1.21
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52
68382	alpha-mannosidase	+	3.2.1.24
68382	alpha-fucosidase	-	3.2.1.51

API coryne

@ref	NIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG
21418	-	-	-	+	-	-	+	+/-	-	+	+	-	-	-	-	-	-	-	-

API zym

@ref	Alkaline phosphatase	Esterase	Esterase Lipase	Lipase	Leucine arylamidase	Valine arylamidase	Cystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
21418	+	+	+/-	-	+	+	+/-	+	+	+	+	-	-	-	+	+	+	+	-

Isolation, sampling and environmental information

isolation

@ref: 10449
sample type: woodland bluff soil
geographic location: Alabama, Lynn, Rainbow Bluff
country: USA
origin.country: USA
continent: North America

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

taxonmaps

@ref: 69479
File name: preview.99_6592.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_109;98_2818;99_6592&stattab=map
Last taxonomy: Streptomyces
16S sequence: EF657884
Sequence Identity:
Total samples: 54
soil counts: 16
animal counts: 2
plant counts: 36

Safety information

risk assessment

@ref	biosafety level	biosafety level comment
10449	1	Risk group (German classification)
21418	L1	German classification

Sequence information

16S sequences

@ref: 10449
description: Streptomyces scopuliridis RB72 16S ribosomal RNA gene, partial sequence
accession: EF657884
length: 1376
database: ena
NCBI tax ID: 1440053

GC content

@ref: 10449
GC-content: 70.3
method: sequence analysis

External links

@ref: 10449

culture collection no.: DSM 41917, NRRL B-24574, RB 72

straininfo link

@ref: 85478
straininfo: 397664

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	20870885	Streptomyces scopuliridis sp. nov., a bacteriocin-producing soil streptomycete.	Farris MH, Duffy C, Findlay RH, Olson JB	Int J Syst Evol Microbiol	10.1099/ijs.0.023192-0	2010	Alabama, Bacterial Typing Techniques, Bacteriocins/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Streptomyces/classification/genetics/isolation & purification/metabolism	Metabolism
Phylogeny	24984801	Streptomyces lushanensis sp. nov., a novel actinomycete with anti-cyanobacterial activity.	Zhang BH, Cheng J, Chen W, Li HQ, Yang JY, Park DJ, Kim CJ, Shen R, Duan YQ, Li WJ	J Antibiot (Tokyo)	10.1038/ja.2014.85	2014	Anti-Bacterial Agents/isolation & purification/pharmacology, Base Composition, China, Cyanobacteria/drug effects, DNA, Bacterial/genetics, Fatty Acids/chemistry/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry/isolation & purification, Phylogeny, RNA, Ribosomal, 16S/genetics, Soil Microbiology, Spores, Bacterial/chemistry, Streptomyces/*classification/genetics/isolation & purification	Enzymology
Phylogeny	25663056	Streptomyces maoxianensis sp. nov., a novel actinomycete isolated from soil in Maoxian, China.	Guan X, Liu C, Zhao J, Fang B, Zhang Y, Li L, Jin P, Wang X, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-015-0403-9	2015	Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Molecular Sequence Data, Phospholipids/chemistry/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Soil Microbiology, Streptomyces/classification/genetics/*isolation & purification/metabolism	Metabolism
Phylogeny	26608172	Streptomyces formicae sp. nov., a novel actinomycete isolated from the head of Camponotus japonicus Mayr.	Bai L, Liu C, Guo L, Piao C, Li Z, Li J, Jia F, Wang X, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-015-0628-7	2015	Animals, Ants/microbiology, China, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Head/microbiology, Molecular Sequence Data, Phylogeny, Streptomyces/classification/genetics/isolation & purification	Genetics
Phylogeny	30993571	Streptomyces corynorhini sp. nov., isolated from Townsend's big-eared bats (Corynorhinus townsendii).	Hamm PS, Caimi NA, Northup DE, Valdez EW, Buecher DC, Dunlap CA, Labeda DP, Porras-Alfaro A	Antonie Van Leeuwenhoek	10.1007/s10482-019-01261-z	2019	Animals, Bacterial Typing Techniques, Base Composition, Chiroptera/microbiology, Metabolic Networks and Pathways/genetics, Microscopy, Electron, Scanning, Multilocus Sequence Typing, New Mexico, Nucleic Acid Hybridization, Phylogeny, Spores, Bacterial/ultrastructure, Streptomyces/classification/genetics/*isolation & purification/physiology, Whole Genome Sequencing	Enzymology
Phylogeny	31674894	Streptomyces paludis sp. nov., isolated from an alpine wetland soil.	Zhao J, Tang X, Li K, Guo Y, Feng M, Gao J	Int J Syst Evol Microbiol	10.1099/ijsem.0.003821	2020	Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Multilocus Sequence Typing, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Streptomyces/classification/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry, Wetlands	Transcriptome
Phylogeny	33783591	Streptomyces liangshanensis sp. nov., a novel actinomycete isolated from rhizosphere soil of Fagopyrum tataricum.	Guo YH, Tang XK, Hu SR, Zhou ML, Gao J	Arch Microbiol	10.1007/s00203-021-02294-7	2021	Actinobacteria/genetics, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fagopyrum/microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, Soil Microbiology, Species Specificity, *Streptomyces/classification/genetics	Transcriptome

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
10449	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41917)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-41917
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
21418	Wink, J.		http://www.dsmz.de/microorganisms/wink_pdf/DSM41917.pdf	Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg		10.1093/nar/gkaa1025	BRENDA, the ELIXIR core data resource in 2021: new developments and updates	Nucleic Acids Res. 49: D498-D508 2020	33211880
68379				Automatically annotated from API Coryne
68382				Automatically annotated from API zym
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
85478	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID397664.1	StrainInfo: A central database for resolving microbial strain identifiers