Strain identifier

BacDive ID: 16436

Type strain: No

Species: Streptomyces hygroscopicus subsp. enhygrus

Strain history: CIP <- 2000, IFO <- NRRL <- The Upjohn Co.: strain UC 2397

NCBI tax ID(s): 285529 (subspecies)

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General

@ref: 10447

BacDive-ID: 16436

DSM-Number: 41913

keywords: 16S sequence, obligate aerobe, mesophilic, Gram-positive, filament-shaped

description: Streptomyces hygroscopicus subsp. enhygrus DSM 41913 is an obligate aerobe, mesophilic, Gram-positive Actinobacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 285529
  • Matching level: subspecies

strain history

@refhistory
10447<- M. Goodfellow, Univ. Newcastle <- The Upjohn Company; UC 2397 <- L. E. Johnson and A. Dietz
120017CIP <- 2000, IFO <- NRRL <- The Upjohn Co.: strain UC 2397

doi: 10.13145/bacdive16436.20230509.8.1

Name and taxonomic classification

@ref: 10447

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces hygroscopicus subsp. enhygrus

full scientific name: Streptomyces hygroscopicus subsp. enhygrus Dietz

type strain: no

Morphology

cell morphology

  • @ref: 120017
  • gram stain: positive
  • cell shape: filament-shaped
  • motility: no

colony morphology

  • @ref: 120017

multimedia

  • @ref: 10447
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_41913.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10447GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
40442MEDIUM 128 - for Nocardia brevicatena, Nocardia flavorosea subsp. fusca and Streptomyces ambofaciensyesDistilled water make up to (1000.000 ml);Maltose (10.000 g);Agar (20.000g);Yeast extract (1.000 g);Beef extract (1.000 g);Casamino acids (2.000 g)
10447ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
10447STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
120017CIP Medium 129yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=129

culture temp

@refgrowthtypetemperaturerange
40442positivegrowth30mesophilic
10447positivegrowth28mesophilic
120017positivegrowth25-37mesophilic
120017nogrowth10psychrophilic
120017nogrowth41thermophilic
120017nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120017
  • oxygen tolerance: obligate aerobe

compound production

  • @ref: 10447
  • compound: scopafungin

halophily

@refsaltgrowthtested relationconcentration
120017NaClpositivegrowth0-4 %
120017NaClnogrowth6 %
120017NaClnogrowth8 %
120017NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120017esculin+hydrolysis4853
120017hippurate+hydrolysis606565
120017nitrate-reduction17632
120017nitrite-reduction16301
120017nitrate-respiration17632

antibiotic resistance

  • @ref: 120017
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 120017
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12001715688acetoin-
12001717234glucose-

enzymes

@refvalueactivityec
120017oxidase-
120017beta-galactosidase+3.2.1.23
120017alcohol dehydrogenase-1.1.1.1
120017gelatinase+/-
120017amylase-
120017DNase-
120017caseinase+3.4.21.50
120017catalase+1.11.1.6
120017tween esterase+
120017gamma-glutamyltransferase+2.3.2.2
120017lecithinase-
120017lipase+
120017lysine decarboxylase-4.1.1.18
120017ornithine decarboxylase-4.1.1.17
120017phenylalanine ammonia-lyase-4.3.1.24
120017protease-
120017tryptophan deaminase-
120017urease+3.5.1.5

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120017-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120017+++++-+-+++++++++++++++++-+----+-+------++-++---++++-+++-+-++---------+-++-----++-------+++++++----

Isolation, sampling and environmental information

isolation

@refsample type
10447soil
120017Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 120017
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces hygroscopicus subsp. enhygrus gene for 16S rRNA, partial sequence, strain: NBRC 13978AB1845581478ena285529
20218Streptomyces castelarensis strain NRRL 3664 sub-species enhygrus 16S ribosomal RNA gene, partial sequenceDQ4425101484ena285529
20218Streptomyces castelarensis strain NRRL 3664 16S ribosomal RNA gene, partial sequenceEF4087341450ena260811

External links

@ref: 10447

culture collection no.: DSM 41913, JCM 4870, KCC S-0870, NBRC 13978, NRRL 3664, UC 2397, CIP 106835, IFO 13978

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10447Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41913)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41913
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40442Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19009
68371Automatically annotated from API 50CH acid
120017Curators of the CIPCollection of Institut Pasteur (CIP 106835)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106835