Strain identifier

BacDive ID: 164197

Type strain: Yes

Species: Alteribacter natronophilus

Strain Designation: M30

Strain history: R. J. Menes; Univ. de la República, Uruguay; M30.

NCBI tax ID(s): 2583810 (species)

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General

@ref: 67770

BacDive-ID: 164197

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, moderately halophilic, heterotroph, spore-forming, mesophilic, Gram-positive, colony-forming

description: Alteribacter natronophilus M30 is an obligate aerobe, moderately halophilic, heterotroph bacterium that forms circular colonies and was isolated from Sediment at Lake Magadi, a soda lake.

NCBI tax id

  • NCBI tax id: 2583810
  • Matching level: species

strain history

  • @ref: 67770
  • history: R. J. Menes; Univ. de la República, Uruguay; M30.

doi: 10.13145/bacdive164197.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Alteribacter
  • species: Alteribacter natronophilus
  • full scientific name: Alteribacter natronophilus (Menes et al. 2020) Gupta et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus natronophilus

@ref: 67770

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Alteribacter

species: Alteribacter natronophilus

full scientific name: Alteribacter natronophilus (Menes et al. 2020) Gupta et al. 2020

strain designation: M30

type strain: yes

Morphology

cell morphology

@refgram stainconfidencemotilitycell lengthcell widthcell shape
69480positive100
6948093.336yes
69523negativeno1.5-2.5 µm0.5-0.8 µmrod-shaped

colony morphology

  • @ref: 69523
  • colony size: 2.0-3.0 mm
  • colony color: beige
  • colony shape: circular
  • incubation period: 2 days
  • medium used: Trypticase Soy Agar (TSA)

Culture and growth conditions

culture medium

@refnamegrowthcomposition
69523Trypticase Soy Agar (TSA)yes2.0% NaCl w/v, pH 10.0
69523Trypticase Soy Agar (TSA)nopH 7.0-7.5, NaCl 0% (w/v)
69523Trypticase Soy Agar (TSA)yespH 10, NaCl 2.0% (w/v)
69523Nutrient agar (NA)nopH 7.0-7.5, NaCl 0% (w/v)
69523R2AnopH 7.0-7.5, NaCl 0% (w/v)
69523Nutrient brothnopH 7.0-7.5, NaCl 0% (w/v)
69523plate count AgaryespH 10, NaCl 2.0% (w/v)
69523plate count agarnopH 7.0-7.5, NaCl 0% (w/v)
69523Nutrient agar (NA)yespH 10, NaCl 2.0% (w/v)
69523R2AyespH 10, NaCl 2.0% (w/v)
69523nutrient brothyespH 10, NaCl 2.0% (w/v)

culture temp

@refgrowthtypetemperaturerange
67770positivegrowth28mesophilic
69523positivegrowth12.0-43.0
69523positiveoptimum25.0-30.0mesophilic

culture pH

@refabilitytypepH
69523positivegrowth8.0-12.0
69523positiveoptimum9.5-10.0

Physiology and metabolism

oxygen tolerance

  • @ref: 69523
  • oxygen tolerance: obligate aerobe

nutrition type

  • @ref: 69523
  • type: heterotroph

spore formation

@refspore formationconfidencespore descriptiontype of spore
69480yes100
69481yes99
69523yescentral, ellipsoidal endospores in unswollen sporangiaendospore

halophily

@refhalophily levelsaltgrowthtested relationconcentration
69523moderately halophilicNaClpositivegrowth0.5-15.0 %(w/v)
69523NaClpositiveoptimum5 %(w/v)

observation

@refobservation
67770quinones: MK-7, MK-6
69523The polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and one unidentified phospholipid.
69523The cell-wall peptidoglycan contains meso-diaminopimelic acid.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6952317234glucose-anaerobic growth
695232-oxogluconate-builds acid from
6952327613amygdalin-builds acid from
6952318305arbutin-builds acid from
6952317057cellobiose-builds acid from
6952317108D-arabinose-builds acid from
6952318333D-arabitol-builds acid from
6952315824D-fructose-builds acid from
6952328847D-fucose-builds acid from
6952362318D-lyxose-builds acid from
6952316988D-ribose-builds acid from
6952317924D-sorbitol-builds acid from
6952316443D-tagatose-builds acid from
6952365327D-xylose-builds acid from
6952317113erythritol-builds acid from
6952316813galactitol-builds acid from
6952328066gentiobiose-builds acid from
6952324265gluconate-builds acid from
6952328087glycogen-builds acid from
6952315443inulin-builds acid from
6952330849L-arabinose-builds acid from
6952318403L-arabitol-builds acid from
6952318287L-fucose-builds acid from
6952362345L-rhamnose-builds acid from
6952317266L-sorbose-builds acid from
6952365328L-xylose-builds acid from
6952317716lactose-builds acid from
695236731melezitose-builds acid from
69523320061methyl alpha-D-glucopyranoside-builds acid from
6952343943methyl alpha-D-mannoside-builds acid from
6952374863methyl beta-D-xylopyranoside-builds acid from
6952317268myo-inositol-builds acid from
69523506227N-acetylglucosamine-builds acid from
6952316634raffinose-builds acid from
6952315963ribitol-builds acid from
6952317814salicin-builds acid from
6952332528turanose-builds acid from
6952317151xylitol-builds acid from
69523168102-oxoglutarate-carbon source
6952330089acetate-carbon source
6952317968butyrate-carbon source
6952317057cellobiose-carbon source
6952316947citrate-carbon source
6952318333D-arabitol-carbon source
6952315824D-fructose-carbon source
6952315740formate-carbon source
6952315428glycine-carbon source
6952330849L-arabinose-carbon source
6952317561L-cysteine-carbon source
6952318050L-glutamine-carbon source
6952318019L-lysine-carbon source
6952362345L-rhamnose-carbon source
69523506227N-acetylglucosamine-carbon source
6952317272propionate-carbon source
6952316634raffinose-carbon source
6952329806fumarate-electron acceptor
6952317632nitrate-electron acceptor
6952316301nitrite-electron acceptor
6952316991dna-hydrolysis
6952361995lecithin-hydrolysis
6952353424tween 20-hydrolysis
6952353425tween 60-hydrolysis
6952353426tween 80-hydrolysis
6952316199urea-hydrolysis
6952315428glycine-nitrogen source
6952317561L-cysteine-nitrogen source
6952318050L-glutamine-nitrogen source
6952318019L-lysine-nitrogen source
6952317632nitrate-reduction
6952316301nitrite-reduction
69523581435-dehydro-D-gluconate+builds acid from
6952312936D-galactose+builds acid from
6952317634D-glucose+builds acid from
6952316899D-mannitol+builds acid from
6952316024D-mannose+builds acid from
69523esculin hydrolysate+builds acid from
6952317754glycerol+builds acid from
6952317306maltose+builds acid from
6952328053melibiose+builds acid from
6952328017starch+builds acid from
6952317992sucrose+builds acid from
6952327082trehalose+builds acid from
6952312936D-galactose+carbon source
6952317634D-glucose+carbon source
6952316899D-mannitol+carbon source
6952365327D-xylose+carbon source
6952330921glutarate+carbon source
6952317754glycerol+carbon source
6952328087glycogen+carbon source
6952316977L-alanine+carbon source
6952316467L-arginine+carbon source
6952317196L-asparagine+carbon source
6952329991L-aspartate+carbon source
6952317191L-isoleucine+carbon source
6952315729L-ornithine+carbon source
6952317295L-phenylalanine+carbon source
6952317115L-serine+carbon source
6952316828L-tryptophan+carbon source
6952316414L-valine+carbon source
6952325115malate+carbon source
6952317306maltose+carbon source
6952328053melibiose+carbon source
6952327082trehalose+carbon source
69523casein+hydrolysis
695234853esculin+hydrolysis
695235291gelatin+hydrolysis
6952328017starch+hydrolysis
6952316977L-alanine+nitrogen source
6952316467L-arginine+nitrogen source
6952317196L-asparagine+nitrogen source
6952329991L-aspartate+nitrogen source
6952317191L-isoleucine+nitrogen source
6952315729L-ornithine+nitrogen source
6952317295L-phenylalanine+nitrogen source
6952317115L-serine+nitrogen source
6952316828L-tryptophan+nitrogen source
6952316414L-valine+nitrogen source
6952318186tyrosine+/-hydrolysis

metabolite production

  • @ref: 69523
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 69523
  • Chebi-ID: 35581
  • metabolite: indole
  • voges-proskauer-test: -
  • methylred-test: -
  • citrate test: -

enzymes

@refvalueactivityec
69523alkaline phosphatase+3.1.3.1
69523cellulase+3.2.1.4
69523esterase Lipase (C 8)+
69523leucine arylamidase+3.4.11.1
69523alpha-chymotrypsin+3.4.21.1
69523alpha-glucosidase+3.2.1.20
69523beta-galactosidase+3.2.1.23
69523endo-1,4-beta-xylanase+3.2.1.8
69523acid phosphatase-3.1.3.2
69523cystine arylamidase-3.4.11.3
69523esterase (C 4)-
69523lipase (C 14)-
69523alpha-fucosidase-3.2.1.51
69523alpha-galactosidase-3.2.1.22
69523beta-glucosidase-3.2.1.21
69523beta-glucuronidase-3.2.1.31
69523lipase-
69523naphthol-AS-BI-phosphohydrolase-
69523N-acetyl-beta-glucosaminidase-3.2.1.52
69523alpha-mannosidase-3.2.1.24
695232-nitrophenyl beta-D-galactopyranoside+3.2.1.52
69523phenylalanine deaminase-4.3.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling datelatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
67770Sediment at Lake Magadi, a soda lakeArusha regionTanzaniaTZAAfrica
69523sediment sample of upper 3.0-5.0 cm layer of Lake MagadiLake Magadi, Arusha regionTanzaniaTZAAfrica2006-3.183335.5333TSAsupplemented with 2% NaCl, pH 102 days30

Sequence information

16S sequences

  • @ref: 67770
  • description: Bacillus sp. (in: Bacteria) strain M30 16S ribosomal RNA gene, partial sequence
  • accession: MG189964
  • length: 1399
  • database: ena
  • NCBI tax ID: 2583810

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
67770Alteribacter natronophilus M30GCA_006007885scaffoldncbi2583810
69523genome sequenceVCRO01000000ncbi2583810

GC content

@refGC-contentmethod
6777048.9genome sequence analysis
6952348.89genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes99no
motileyes76.498no
flagellatedno50no
gram-positiveyes89.009no
anaerobicno97.694no
aerobicyes85.106no
halophileyes91.785no
spore-formingyes88.605no
thermophileno93.813yes
glucose-utilyes88.434no
glucose-fermentno93.6no

External links

@ref: 67770

culture collection no.: JCM 32118, CGMCC 1.16739, MCC 3010

literature

  • topic: Phylogeny
  • Pubmed-ID: 31613745
  • title: Bacillus natronophilus sp. nov., an alkaliphilic bacterium isolated from a soda lake.
  • authors: Menes RJ, Machin EV, Iriarte A, Langleib M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003792
  • year: 2020
  • mesh: *Alkalies, Bacillus/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Lakes/chemistry/*microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Tanzania, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/url
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
69523Rodolfo Javier Menes, Eliana Valentina Machin, Andrés Iriarte, Mauricio LangleibBacillus natronophilus sp. nov., an alkaliphilic bacterium isolated from a soda lake10.1099/ijsem.0.003792