Strain identifier

BacDive ID: 16417

Type strain: Yes

Species: Streptomyces polyantibioticus

Strain Designation: SPR

Strain history: <- P. R. Meyers

NCBI tax ID(s): 1144882 (strain), 340148 (species)

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General

@ref: 12198

BacDive-ID: 16417

DSM-Number: 44925

keywords: 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces polyantibioticus SPR is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil from riverbanks.

NCBI tax id

NCBI tax idMatching level
1144882strain
340148species

strain history

  • @ref: 12198
  • history: <- P. R. Meyers

doi: 10.13145/bacdive16417.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces polyantibioticus
  • full scientific name: Streptomyces polyantibioticus Le Roes-Hill and Meyers 2009

@ref: 12198

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces polyantibioticus

full scientific name: Streptomyces polyantibioticus le Roes-Hill and Meyers 2009

strain designation: SPR

type strain: yes

Morphology

cell morphology

  • @ref: 29098
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
19888Mahagony brown10-14 daysISP 2
19888Pure white10-14 daysISP 3
19888Signal white10-14 daysISP 4
19888Graphite black10-14 daysISP 5
19888Black brown10-14 daysISP 6
1988810-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19888yesAerial MyceliumCreamISP 2
19888yesAerial MyceliumCreamISP 3
19888yesAerial MyceliumCreamISP 4
19888noISP 5
19888noISP 6
19888yesAerial MyceliumBlack brownISP 7

pigmentation

  • @ref: 29098
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12198STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
12198GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19888ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19888ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19888ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19888ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19888ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19888ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
12198TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
12198positivegrowth28mesophilic
19888positiveoptimum28mesophilic
29098positivegrowth04-30
29098positiveoptimum17psychrophilic

culture pH

  • @ref: 29098
  • ability: positive
  • type: growth
  • pH: <4.3

Physiology and metabolism

spore formation

  • @ref: 29098
  • spore formation: yes

halophily

  • @ref: 29098
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <7 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2909829016arginine+carbon source
2909817057cellobiose+carbon source
2909828757fructose+carbon source
2909828260galactose+carbon source
2909817234glucose+carbon source
2909827570histidine+carbon source
29098182404-hydroxy-L-proline+carbon source
2909817716lactose+carbon source
2909837684mannose+carbon source
2909828053melibiose+carbon source
2909828044phenylalanine+carbon source
2909816634raffinose+carbon source
2909833942ribose+carbon source
2909817814salicin+carbon source
2909817822serine+carbon source
2909826986threonine+carbon source
2909827082trehalose+carbon source
2909853426tween 80+carbon source
2909818222xylose+carbon source
290984853esculin+hydrolysis
2909817632nitrate+reduction

metabolite production

  • @ref: 29098
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

enzymes

@refvalueactivityec
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19888+++-++---++-+-++++-

Isolation, sampling and environmental information

isolation

  • @ref: 12198
  • sample type: soil from riverbanks
  • geographic location: Umgeni River, KwaZulu-Natal Province
  • country: South Africa
  • origin.country: ZAF
  • continent: Africa

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#River (Creek)
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 12198
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 12198
  • description: Streptomyces polyantibioticus strain SPR 16S ribosomal RNA gene, partial sequence
  • accession: DQ141528
  • length: 1459
  • database: ena
  • NCBI tax ID: 1144882

GC content

@refGC-contentmethod
1219874.4thermal denaturation, midpoint method (Tm)
2909874.4

External links

@ref: 12198

culture collection no.: DSM 44925, NRRL B-24448

straininfo link

  • @ref: 85461
  • straininfo: 286032

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19502306Streptomyces polyantibioticus sp. nov., isolated from the banks of a river.le Roes-Hill M, Meyers PRInt J Syst Evol Microbiol10.1099/ijs.0.006171-02009*Antibiosis, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Gram-Negative Bacteria/*growth & development, Gram-Positive Bacteria/*growth & development, Microbial Sensitivity Tests/methods, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Rivers, Sequence Analysis, DNA, *Soil Microbiology, South Africa, Species Specificity, Streptomyces/*classification/genetics/isolation & purification/physiologyGenetics
Phylogeny28185026Streptomyces aridus sp. nov., isolated from a high altitude Atacama Desert soil and emended description of Streptomyces noboritoensis Isono et al. 1957.Idris H, Labeda DP, Nouioui I, Castro JF, Del Carmen Montero-Calasanz M, Bull AT, Asenjo JA, Goodfellow MAntonie Van Leeuwenhoek10.1007/s10482-017-0838-22017Altitude, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Desert Climate, Fatty Acids/analysis, Microscopy, Electron, Scanning, Multilocus Sequence Typing, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification/physiologyEnzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
12198Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44925)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44925
19888Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44925.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29098Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2552728776041
68382Automatically annotated from API zym
85461Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID286032.1StrainInfo: A central database for resolving microbial strain identifiers