Strain identifier

BacDive ID: 16416

Type strain: Yes

Species: Streptomyces lunalinharesii

Strain Designation: RCQ1071

Strain history: CIP <- 2005, R. F. de Souza, UFRJ Univ., Rio de Janeiro, Brazil: strain RCQ1071

NCBI tax ID(s): 333384 (species)

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General

@ref: 10538

BacDive-ID: 16416

DSM-Number: 41876

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Streptomyces lunalinharesii RCQ1071 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from acid orthic ferralsol under cerrado vegetation.

NCBI tax id

  • NCBI tax id: 333384
  • Matching level: species

strain history

@refhistory
10538<- R. F. de Souza <- R. C. Gomes; RCQ1071
383392005, R. F. de Souza, Rio de Janeiro, Brazil: strain RCQ1071
67770CIP 108852 <-- R. F. de Souza RCQ1071.
120674CIP <- 2005, R. F. de Souza, UFRJ Univ., Rio de Janeiro, Brazil: strain RCQ1071

doi: 10.13145/bacdive16416.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces lunalinharesii
  • full scientific name: Streptomyces lunalinharesii de Souza et al. 2008

@ref: 10538

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces lunalinharesii

full scientific name: Streptomyces lunalinharesii de Souza et al. 2008

strain designation: RCQ1071

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
32609positiverod-shaped
120674positiverod-shapedno

colony morphology

@refcolony colorincubation periodmedium used
18727Broom beige10-14 daysISP 2
18727Beige (1001)10-14 daysISP 3
18727Beige (1001)10-14 daysISP 4
18727Beige (1001)10-14 daysISP 5
18727Corn yellow (1006)10-14 daysISP 6
18727Corn yellow (1006)10-14 daysISP 7
120674

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
18727yesAerial MyceliumBasalt grey (7012)ISP 2
18727yesAerial MyceliumBasalt grey (7012)ISP 3
18727yesAerial MyceliumBasalt grey (7012)ISP 4
18727yesAerial MyceliumCream (9001)ISP 5
18727yesAerial MyceliumCream (9001)ISP 6
18727yesAerial MyceliumCream (9001)ISP 7

pigmentation

  • @ref: 32609
  • production: yes

multimedia

  • @ref: 10538
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_41876.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10538GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18727ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18727ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18727ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18727ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18727ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18727ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
38339MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
120674CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
120674CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
10538positivegrowth28mesophilic
18727positiveoptimum28mesophilic
32609positivegrowth45thermophilic
32609positiveoptimum45thermophilic
38339positivegrowth30mesophilic
67770positivegrowth28mesophilic
120674positivegrowth10-37
120674nogrowth41thermophilic
120674nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32609aerobe
120674facultative anaerobe

spore formation

  • @ref: 32609
  • spore formation: yes

halophily

  • @ref: 120674
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

observation

  • @ref: 32609
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3260922599arabinose+carbon source
3260926546rhamnose+carbon source
3260917992sucrose+carbon source
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
12067416947citrate+carbon source
1206744853esculin+hydrolysis
120674606565hippurate+hydrolysis
12067417632nitrate-reduction
12067416301nitrite-reduction
12067417632nitrate+respiration

metabolite production

  • @ref: 120674
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12067415688acetoin-
12067417234glucose-

enzymes

@refvalueactivityec
32609urease+3.5.1.5
120674oxidase-
120674beta-galactosidase-3.2.1.23
120674alcohol dehydrogenase-1.1.1.1
120674gelatinase-
120674amylase+
120674DNase+
120674caseinase+3.4.21.50
120674catalase+1.11.1.6
120674tween esterase-
120674gamma-glutamyltransferase+2.3.2.2
120674lecithinase-
120674lipase-
120674lysine decarboxylase-4.1.1.18
120674ornithine decarboxylase-4.1.1.17
120674phenylalanine ammonia-lyase-4.3.1.24
120674protease+
120674tryptophan deaminase-
120674urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18727+++++++++++---++++-
120674+++++++--++---++++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120674+/-+/---+/---+/--+/-+/-+/-+/----+/-+/----+/-+/-+/-+/-+/-+/-+/----+/----+/-+/-------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120674+++++----+--------++-----------+++---+--+------++++--++----++++---------++-+-++++---+-+-++++-++-+++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
10538acid orthic ferralsol under cerrado vegetationCentral PlateauBrazilBRAMiddle and South America
67770Acid orthic ferralsol under cerrado vegetationBrazilBRAMiddle and South America
120674Environment, Acid orthic soil ferralsol under cerrado vegetationBrazilBRAMiddle and South America

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants
#Condition#Acidic

taxonmaps

  • @ref: 69479
  • File name: preview.99_2970.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_103;97_110;98_119;99_2970&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: DQ094838
  • Sequence Identity:
  • Total samples: 1706
  • soil counts: 1460
  • aquatic counts: 61
  • animal counts: 112
  • plant counts: 73

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
105381Risk group (German classification)
1206741Risk group (French classification)

Sequence information

16S sequences

  • @ref: 10538
  • description: Streptomyces lunalinharesii strain RCQ1071 16S ribosomal RNA gene, partial sequence
  • accession: DQ094838
  • length: 1480
  • database: ena
  • NCBI tax ID: 333384

External links

@ref: 10538

culture collection no.: DSM 41876, ATCC BAA 1231, CIP 108852, JCM 16374

straininfo link

  • @ref: 85460
  • straininfo: 297711

literature

  • topic: Phylogeny
  • Pubmed-ID: 19060056
  • title: Streptomyces lunalinharesii sp. nov., a chitinolytic streptomycete isolated from cerrado soil in Brazil.
  • authors: de Souza RF, Coelho RR, Macrae A, Soares RM, Nery Dda C, Semedo LT, Alviano CS, Gomes RC
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65768-0
  • year: 2008
  • mesh: Brazil, Chitin/*metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Species Specificity, Streptomyces/*classification/genetics/*physiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
10538Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41876)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41876
18727Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41876.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32609Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2882228776041
38339Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6528
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
85460Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297711.1StrainInfo: A central database for resolving microbial strain identifiers
120674Curators of the CIPCollection of Institut Pasteur (CIP 108852)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108852