Strain identifier

BacDive ID: 16406

Type strain: Yes

Species: Streptomyces durmitorensis

Strain Designation: MS 405

Strain history: DSM 41863 <-- M. Savic MS405.

NCBI tax ID(s): 319947 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 10643

BacDive-ID: 16406

DSM-Number: 41863

keywords: 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive

description: Streptomyces durmitorensis MS 405 is a spore-forming, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 319947
  • Matching level: species

strain history

@refhistory
10643<- M. Savic; MS 405
67770DSM 41863 <-- M. Savic MS405.

doi: 10.13145/bacdive16406.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces durmitorensis
  • full scientific name: Streptomyces durmitorensis Savic et al. 2007

@ref: 10643

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces durmitorensis

full scientific name: Streptomyces durmitorensis Savic et al. 2007

strain designation: MS 405

type strain: yes

Morphology

cell morphology

  • @ref: 32070
  • gram stain: positive

colony morphology

@refcolony colorincubation periodmedium used
19824Cream10-14 daysISP 2
19824Cream10-14 daysISP 3
19824Grey white10-14 daysISP 4
19824Cream10-14 daysISP 5
19824Cream10-14 daysISP 6
19824Cream10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19824yesAerial MyceliumCreamISP 2
19824yesAerial MyceliumCreamISP 3
19824yesAerial MyceliumCreamISP 4
19824yesAerial MyceliumGolden yellowISP 5
19824noISP 6
19824noISP 7

pigmentation

  • @ref: 32070
  • production: yes

multimedia

  • @ref: 10643
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_41863.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10643GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19824ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19824ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19824ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19824ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19824ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19824ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
10643ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
10643STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
10643positivegrowth28mesophilic
32070positivegrowth10-37
32070positiveoptimum30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 32070
  • spore formation: yes

compound production

  • @ref: 10643
  • compound: immunosuppressor

halophily

@refsaltgrowthtested relationconcentration
32070NaClpositivegrowth0-9 %
32070NaClpositiveoptimum4.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1982417234glucose+
1982422599arabinose+
1982417992sucrose-
1982418222xylose+
1982417268myo-inositol+
1982429864mannitol+
1982428757fructose+
1982426546rhamnose+
1982416634raffinose+
1982462968cellulose+
3207017057cellobiose+carbon source
3207028757fructose+carbon source
3207028260galactose+carbon source
3207017234glucose+carbon source
3207017754glycerol+carbon source
3207029864mannitol+carbon source
3207037684mannose+carbon source
3207028053melibiose+carbon source
3207016634raffinose+carbon source
3207026546rhamnose+carbon source
3207017992sucrose+carbon source
3207027082trehalose+carbon source
3207018222xylose+carbon source
3207017632nitrate+reduction

enzymes

@refvalueactivityec
32070catalase+1.11.1.6
32070gelatinase+
32070urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19824+++-++-++++++-++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
10643soilNational Park DurmitorSerbia and MontenegroSRBEurope
67770Soil at the Durmitor National ParkMontenegroMNEEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
106431Risk group (German classification)
198241Risk group (German classification)

Sequence information

16S sequences

  • @ref: 10643
  • description: Streptomyces durmitorensis strain MS405 16S ribosomal RNA gene, partial sequence
  • accession: DQ067287
  • length: 1517
  • database: ena
  • NCBI tax ID: 319947

GC content

  • @ref: 10643
  • GC-content: 72

External links

@ref: 10643

culture collection no.: DSM 41863, CIP 108995, JCM 16011

straininfo link

  • @ref: 85453
  • straininfo: 297705

literature

  • topic: Phylogeny
  • Pubmed-ID: 17766883
  • title: Streptomyces durmitorensis sp. nov., a producer of an FK506-like immunosuppressant.
  • authors: Savic M, Bratic I, Vasiljevic B
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64913-0
  • year: 2007
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA, Ribosomal Spacer/chemistry/genetics, Genes, rRNA, Immunosuppressive Agents/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymorphism, Genetic, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, *Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification/metabolism, Yugoslavia
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
10643Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41863)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41863
19824Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41863.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32070Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2831728776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
85453Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297705.1StrainInfo: A central database for resolving microbial strain identifiers