Strain identifier

BacDive ID: 16403

Type strain: Yes

Species: Streptomyces drozdowiczii

Strain Designation: M7a

Strain history: CIP <- 2003, L. Teixeira , Brazil Univ., Rio de Janeiro, Brazil: strain M7a

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 10639

BacDive-ID: 16403

DSM-Number: 41857

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic

description: Streptomyces drozdowiczii M7a is an obligate aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil .

NCBI tax id

NCBI tax idMatching level
202862species
1302724strain

strain history

@refhistory
10639<- G. P. Manfio; CBMAI 498
331712003, L. Teixeira , Brazil Univ., Rio de Janeiro, Brazil: strain M7a
67770CIP 107837 <-- L. Teixeira M7a.
118974CIP <- 2003, L. Teixeira , Brazil Univ., Rio de Janeiro, Brazil: strain M7a

doi: 10.13145/bacdive16403.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces drozdowiczii
  • full scientific name: Streptomyces drozdowiczii Semêdo et al. 2004

@ref: 10639

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces drozdowiczii

full scientific name: Streptomyces drozdowiczii Semêdo et al. 2004

strain designation: M7a

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no91.32
6948099.737positive
118974nopositiverod-shaped

colony morphology

@refcolony colorincubation periodmedium used
18725Grey brown (8019)10-14 daysISP 2
18725Grey brown (8019)10-14 daysISP 3
18725Grey brown (8019)10-14 daysISP 4
18725Cream (9001)10-14 daysISP 5
18725Beige (1001)10-14 daysISP 6
18725Beige (1001)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
18725yesAerial MyceliumBasalt grey (7012)ISP 2
18725yesAerial MyceliumBasalt grey (7012)ISP 3
18725yesBasalt grey (7012)ISP 4
18725yesAerial MyceliumBeige (1001)ISP 5
18725yesAerial MyceliumBeige (1001)ISP 6
18725yesAerial MyceliumSignal grey (7004)ISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10639GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18725ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18725ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18725ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18725ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18725ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18725ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33171MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
10639ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
10639STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
118974CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
10639positivegrowth28mesophilic
18725positiveoptimum28mesophilic
33171positivegrowth30mesophilic
67770positivegrowth28mesophilic
118974positivegrowth25-41
118974nogrowth10psychrophilic
118974nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 118974
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
30024yes
69481yes100
69480no99.971

halophily

@refsaltgrowthtested relationconcentration
118974NaClpositivegrowth0-2 %
118974NaClnogrowth4 %
118974NaClnogrowth6 %
118974NaClnogrowth8 %
118974NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3002415963ribitol+carbon source
3002422599arabinose+carbon source
3002417057cellobiose+carbon source
3002428757fructose+carbon source
3002428260galactose+carbon source
3002437684mannose+carbon source
3002428053melibiose+carbon source
3002417814salicin+carbon source
3002417992sucrose+carbon source
3002426986threonine+carbon source
300244853esculin+hydrolysis
3002417632nitrate+reduction
11897416947citrate-carbon source
1189744853esculin+hydrolysis
118974606565hippurate-hydrolysis
11897417632nitrate+reduction
11897416301nitrite-reduction
11897417632nitrate-respiration

metabolite production

@refChebi-IDmetaboliteproduction
3002416136hydrogen sulfideyes
11897435581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11897415688acetoin-
11897417234glucose-

enzymes

@refvalueactivityec
30024gelatinase+
67770cellulase3.2.1.4
118974oxidase-
118974beta-galactosidase-3.2.1.23
118974alcohol dehydrogenase-1.1.1.1
118974gelatinase+
118974amylase+
118974DNase-
118974caseinase+3.4.21.50
118974catalase+1.11.1.6
118974tween esterase+
118974gamma-glutamyltransferase+2.3.2.2
118974lecithinase-
118974lipase+
118974lysine decarboxylase-4.1.1.18
118974ornithine decarboxylase-4.1.1.17
118974protease+
118974tryptophan deaminase-
118974urease-3.5.1.5
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18725+++-+----++-+-++++-
118974-++++----+--+--+++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118974+++++-++++++++-++-+-++-++------+-----------------+----++---++--------------+---++---+-+-++++-++-+++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
10639soil (Mata Atlantica Forest)Rio de JaneiroBrazilBRAMiddle and South America
67770Forest soilRio de JaneiroBrazilBRAMiddle and South America
118974Environment, SoilMata AtlanticaBrazilBRAMiddle and South America1994

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
106391Risk group (German classification)
187251German classification
1189741Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces drozdowiczii gene for 16S rRNA, partial sequence, strain: NBRC 101007AB2499571468ena202862
20218Streptomyces drozdowiczii strain NRRL B-24297 16S ribosomal RNA gene, partial sequenceEF6540971500ena202862
30024Streptomyces drozdowiczii 16S ribosomal RNA gene, partial sequenceAF5291991191nuccore202862

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces drozdowiczii JCM 13580GCA_001313325contigncbi1302724
66792Streptomyces drozdowiczii JCM 135801302724.4wgspatric1302724
66792Streptomyces drozdowiczii JCM 135802734481941draftimg1302724

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno90.638no
gram-positiveyes91.044no
anaerobicno98.911no
halophileno90.61no
spore-formingyes65.121yes
thermophileno96.083yes
glucose-utilyes89.675no
aerobicyes93.854no
flagellatedno97.897no
glucose-fermentno82.027no

External links

@ref: 10639

culture collection no.: DSM 41857, CCT 5476, CIP 107837, NBRC 101007, NRRL B-24297, CBMAI 498, JCM 13580, CBMAI 0498

straininfo link

  • @ref: 85450
  • straininfo: 132800

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15280309Streptomyces drozdowiczii sp. nov., a novel cellulolytic streptomycete from soil in Brazil.Semedo LTAS, Gomes RC, Linhares AA, Duarte GF, Nascimento RP, Rosado AS, Margis-Pinheiro M, Margis R, Silva KRA, Alviano CS, Manfio GP, Soares RMA, Linhares LF, Coelho RRRInt J Syst Evol Microbiol10.1099/ijs.0.02844-02004Amino Acids/analysis, Bacterial Typing Techniques, Brazil, Cell Wall/chemistry, Cellulose/*metabolism, DNA, Bacterial/chemistry, DNA, Ribosomal/chemistry, Fatty Acids/analysis, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, *Soil Microbiology, Streptomyces/*classification/cytology/*isolation & purification/metabolismMetabolism
Phylogeny21478394Streptomyces brevispora sp. nov. and Streptomyces laculatispora sp. nov., actinomycetes isolated from soil.Zucchi TD, Kim BY, Kshetrimayum JD, Weon HY, Kwon SW, Goodfellow MInt J Syst Evol Microbiol10.1099/ijs.0.029991-02011Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Microbial Sensitivity Tests, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/chemistry/*classification/genetics/*isolation & purificationGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
10639Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41857)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41857
18725Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41857.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
30024Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604126388
33171Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5392
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
85450Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID132800.1StrainInfo: A central database for resolving microbial strain identifiers
118974Curators of the CIPCollection of Institut Pasteur (CIP 107837)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107837