Strain identifier
BacDive ID: 16403
Type strain:
Species: Streptomyces drozdowiczii
Strain Designation: M7a
Strain history: CIP <- 2003, L. Teixeira , Brazil Univ., Rio de Janeiro, Brazil: strain M7a
NCBI tax ID(s): 1302724 (strain), 202862 (species)
General
@ref: 10639
BacDive-ID: 16403
DSM-Number: 41857
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic
description: Streptomyces drozdowiczii M7a is an obligate aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil .
NCBI tax id
NCBI tax id | Matching level |
---|---|
202862 | species |
1302724 | strain |
strain history
@ref | history |
---|---|
10639 | <- G. P. Manfio; CBMAI 498 |
33171 | 2003, L. Teixeira , Brazil Univ., Rio de Janeiro, Brazil: strain M7a |
67770 | CIP 107837 <-- L. Teixeira M7a. |
118974 | CIP <- 2003, L. Teixeira , Brazil Univ., Rio de Janeiro, Brazil: strain M7a |
doi: 10.13145/bacdive16403.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces drozdowiczii
- full scientific name: Streptomyces drozdowiczii Semêdo et al. 2004
@ref: 10639
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces drozdowiczii
full scientific name: Streptomyces drozdowiczii Semêdo et al. 2004
strain designation: M7a
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | no | 91.32 | ||
69480 | 99.737 | positive | ||
118974 | no | positive | rod-shaped |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
18725 | Grey brown (8019) | 10-14 days | ISP 2 |
18725 | Grey brown (8019) | 10-14 days | ISP 3 |
18725 | Grey brown (8019) | 10-14 days | ISP 4 |
18725 | Cream (9001) | 10-14 days | ISP 5 |
18725 | Beige (1001) | 10-14 days | ISP 6 |
18725 | Beige (1001) | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
18725 | yes | Aerial Mycelium | Basalt grey (7012) | ISP 2 |
18725 | yes | Aerial Mycelium | Basalt grey (7012) | ISP 3 |
18725 | yes | Basalt grey (7012) | ISP 4 | |
18725 | yes | Aerial Mycelium | Beige (1001) | ISP 5 |
18725 | yes | Aerial Mycelium | Beige (1001) | ISP 6 |
18725 | yes | Aerial Mycelium | Signal grey (7004) | ISP 7 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
10639 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
18725 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
18725 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
18725 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
18725 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
18725 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
18725 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
33171 | MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum | yes | Distilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g) | |
10639 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://mediadive.dsmz.de/medium/84 | Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water |
10639 | STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) | yes | https://mediadive.dsmz.de/medium/252 | Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water |
118974 | CIP Medium 57 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
10639 | positive | growth | 28 | mesophilic |
18725 | positive | optimum | 28 | mesophilic |
33171 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
118974 | positive | growth | 25-41 | |
118974 | no | growth | 10 | psychrophilic |
118974 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 118974
- oxygen tolerance: obligate aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
30024 | yes | |
69481 | yes | 100 |
69480 | no | 99.971 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
118974 | NaCl | positive | growth | 0-2 % |
118974 | NaCl | no | growth | 4 % |
118974 | NaCl | no | growth | 6 % |
118974 | NaCl | no | growth | 8 % |
118974 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
30024 | 15963 | ribitol | + | carbon source |
30024 | 22599 | arabinose | + | carbon source |
30024 | 17057 | cellobiose | + | carbon source |
30024 | 28757 | fructose | + | carbon source |
30024 | 28260 | galactose | + | carbon source |
30024 | 37684 | mannose | + | carbon source |
30024 | 28053 | melibiose | + | carbon source |
30024 | 17814 | salicin | + | carbon source |
30024 | 17992 | sucrose | + | carbon source |
30024 | 26986 | threonine | + | carbon source |
30024 | 4853 | esculin | + | hydrolysis |
30024 | 17632 | nitrate | + | reduction |
118974 | 16947 | citrate | - | carbon source |
118974 | 4853 | esculin | + | hydrolysis |
118974 | 606565 | hippurate | - | hydrolysis |
118974 | 17632 | nitrate | + | reduction |
118974 | 16301 | nitrite | - | reduction |
118974 | 17632 | nitrate | - | respiration |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
30024 | 16136 | hydrogen sulfide | yes |
118974 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
118974 | 15688 | acetoin | - | |
118974 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
30024 | gelatinase | + | |
67770 | cellulase | 3.2.1.4 | |
118974 | oxidase | - | |
118974 | beta-galactosidase | - | 3.2.1.23 |
118974 | alcohol dehydrogenase | - | 1.1.1.1 |
118974 | gelatinase | + | |
118974 | amylase | + | |
118974 | DNase | - | |
118974 | caseinase | + | 3.4.21.50 |
118974 | catalase | + | 1.11.1.6 |
118974 | tween esterase | + | |
118974 | gamma-glutamyltransferase | + | 2.3.2.2 |
118974 | lecithinase | - | |
118974 | lipase | + | |
118974 | lysine decarboxylase | - | 4.1.1.18 |
118974 | ornithine decarboxylase | - | 4.1.1.17 |
118974 | protease | + | |
118974 | tryptophan deaminase | - | |
118974 | urease | - | 3.5.1.5 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | alpha-mannosidase | + | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | Control |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
18725 | + | + | + | - | + | - | - | - | - | + | + | - | + | - | + | + | + | + | - | |
118974 | - | + | + | + | + | - | - | - | - | + | - | - | + | - | - | + | + | + | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
118974 | + | + | + | + | + | - | + | + | + | + | + | + | + | + | - | + | + | - | + | - | + | + | - | + | + | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | + | + | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | + | + | - | - | - | + | - | + | - | + | + | + | + | - | + | + | - | + | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
10639 | soil (Mata Atlantica Forest) | Rio de Janeiro | Brazil | BRA | Middle and South America | |
67770 | Forest soil | Rio de Janeiro | Brazil | BRA | Middle and South America | |
118974 | Environment, Soil | Mata Atlantica | Brazil | BRA | Middle and South America | 1994 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Forest |
#Environmental | #Terrestrial | #Soil |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
10639 | 1 | Risk group (German classification) |
18725 | 1 | German classification |
118974 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Streptomyces drozdowiczii gene for 16S rRNA, partial sequence, strain: NBRC 101007 | AB249957 | 1468 | ena | 202862 |
20218 | Streptomyces drozdowiczii strain NRRL B-24297 16S ribosomal RNA gene, partial sequence | EF654097 | 1500 | ena | 202862 |
30024 | Streptomyces drozdowiczii 16S ribosomal RNA gene, partial sequence | AF529199 | 1191 | nuccore | 202862 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Streptomyces drozdowiczii JCM 13580 | GCA_001313325 | contig | ncbi | 1302724 |
66792 | Streptomyces drozdowiczii JCM 13580 | 1302724.4 | wgs | patric | 1302724 |
66792 | Streptomyces drozdowiczii JCM 13580 | 2734481941 | draft | img | 1302724 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
motile | no | 90.638 | no |
gram-positive | yes | 91.044 | no |
anaerobic | no | 98.911 | no |
halophile | no | 90.61 | no |
spore-forming | yes | 65.121 | yes |
thermophile | no | 96.083 | yes |
glucose-util | yes | 89.675 | no |
aerobic | yes | 93.854 | no |
flagellated | no | 97.897 | no |
glucose-ferment | no | 82.027 | no |
External links
@ref: 10639
culture collection no.: DSM 41857, CCT 5476, CIP 107837, NBRC 101007, NRRL B-24297, CBMAI 498, JCM 13580, CBMAI 0498
straininfo link
- @ref: 85450
- straininfo: 132800
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 15280309 | Streptomyces drozdowiczii sp. nov., a novel cellulolytic streptomycete from soil in Brazil. | Semedo LTAS, Gomes RC, Linhares AA, Duarte GF, Nascimento RP, Rosado AS, Margis-Pinheiro M, Margis R, Silva KRA, Alviano CS, Manfio GP, Soares RMA, Linhares LF, Coelho RRR | Int J Syst Evol Microbiol | 10.1099/ijs.0.02844-0 | 2004 | Amino Acids/analysis, Bacterial Typing Techniques, Brazil, Cell Wall/chemistry, Cellulose/*metabolism, DNA, Bacterial/chemistry, DNA, Ribosomal/chemistry, Fatty Acids/analysis, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, *Soil Microbiology, Streptomyces/*classification/cytology/*isolation & purification/metabolism | Metabolism |
Phylogeny | 21478394 | Streptomyces brevispora sp. nov. and Streptomyces laculatispora sp. nov., actinomycetes isolated from soil. | Zucchi TD, Kim BY, Kshetrimayum JD, Weon HY, Kwon SW, Goodfellow M | Int J Syst Evol Microbiol | 10.1099/ijs.0.029991-0 | 2011 | Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Microbial Sensitivity Tests, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/chemistry/*classification/genetics/*isolation & purification | Genetics |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed | ID_cross_reference |
---|---|---|---|---|---|---|---|
10639 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41857) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-41857 | ||||
18725 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM41857.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | ||
30024 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28776041 | 26388 | ||
33171 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/5392 | |||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||||
68382 | Automatically annotated from API zym | ||||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
85450 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID132800.1 | StrainInfo: A central database for resolving microbial strain identifiers | ||||
118974 | Curators of the CIP | Collection of Institut Pasteur (CIP 107837) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107837 |