Strain identifier

BacDive ID: 16386

Type strain: Yes

Species: Streptomyces hebeiensis

Strain history: CIP <- 2003, W.J. Li, Yunnan Univ., Kunming, China: strain YIM 001

NCBI tax ID(s): 229486 (species)

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General

@ref: 10620

BacDive-ID: 16386

DSM-Number: 41837

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-negative, coccus-shaped

description: Streptomyces hebeiensis DSM 41837 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 229486
  • Matching level: species

strain history

@refhistory
10620<- W.-J. Li, YIM
382462003, W.J. Li, Yunnan Univ., Kunming, China: strain YIM 001
67770CIP 107974 <-- W.-J. Li YIM 001.
119853CIP <- 2003, W.J. Li, Yunnan Univ., Kunming, China: strain YIM 001

doi: 10.13145/bacdive16386.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces hebeiensis
  • full scientific name: Streptomyces hebeiensis Xu et al. 2004

@ref: 10620

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces hebeiensis

full scientific name: Streptomyces hebeiensis Xu et al. 2004

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
30065negativecoccus-shapedno
119853positivefilament-shapedno

colony morphology

@refcolony colorincubation periodmedium used
18724Brown green (6008)10-14 daysISP 2
18724Brown green (6008)10-14 daysISP 3
18724Reed green (6013)10-14 daysISP 4
18724Reed green (6013)10-14 daysISP 5
18724Beige (1001)10-14 daysISP 6
18724Red brown (8012)10-14 daysISP 7
119853

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
18724yesAerial MyceliumReed green (6013)ISP 2
18724yesAerial MyceliumReed green (6013)ISP 3
18724yesAerial MyceliumReed green (6013)ISP 4
18724yesAerial MyceliumReed green (6013)ISP 5
18724yesAerial MyceliumWhiteISP 6
18724yesAerial MyceliumWhiteISP 7

pigmentation

  • @ref: 30065
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10620GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18724ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18724ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18724ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18724ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18724ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18724ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
38246MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
10620ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
10620STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
119853CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
10620positivegrowth28mesophilic
18724positiveoptimum28mesophilic
30065positivegrowth27-37mesophilic
30065positiveoptimum32mesophilic
38246positivegrowth30mesophilic
67770positivegrowth28mesophilic
119853positivegrowth25-41
119853nogrowth10psychrophilic
119853nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
30065aerobe
119853obligate aerobe

spore formation

  • @ref: 30065
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
30065NaClpositivegrowth04-07 %
30065NaClpositiveoptimum5.5 %
119853NaClpositivegrowth0-6 %
119853NaClnogrowth8 %
119853NaClnogrowth10 %

observation

@refobservation
30065aggregates in chains
67770quinones: MK-9(H6), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3006522599arabinose+carbon source
3006528757fructose+carbon source
3006528260galactose+carbon source
300655291gelatin+carbon source
3006517754glycerol+carbon source
3006527570histidine+carbon source
30065182404-hydroxy-L-proline+carbon source
3006517716lactose+carbon source
3006517306maltose+carbon source
3006529864mannitol+carbon source
3006537684mannose+carbon source
3006516634raffinose+carbon source
3006526546rhamnose+carbon source
3006530911sorbitol+carbon source
3006517992sucrose+carbon source
3006518222xylose+carbon source
3006517632nitrate+reduction
11985316947citrate+carbon source
1198534853esculin+hydrolysis
119853606565hippurate+hydrolysis
11985317632nitrate-reduction
11985316301nitrite-reduction
11985317632nitrate-respiration

antibiotic resistance

  • @ref: 119853
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 119853
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11985315688acetoin-
11985317234glucose-

enzymes

@refvalueactivityec
30065gelatinase+
119853oxidase-
119853beta-galactosidase+3.2.1.23
119853alcohol dehydrogenase-1.1.1.1
119853gelatinase+
119853amylase+
119853DNase+
119853caseinase+3.4.21.50
119853catalase+1.11.1.6
119853tween esterase-
119853gamma-glutamyltransferase+2.3.2.2
119853lecithinase-
119853lipase-
119853lysine decarboxylase-4.1.1.18
119853ornithine decarboxylase-4.1.1.17
119853phenylalanine ammonia-lyase-4.3.1.24
119853protease+
119853tryptophan deaminase-
119853urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase+3.2.1.21
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18724+---++-++++-+-++++-
119853++--+---+++-+--+-+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119853+++++-+--++++-+++++-++++--+----+-+---+---+-++----++--+++---++------------+-+---++-------++++-++----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
10620soilHebei provinceChinaCHNAsia
67770SoilHebei ProvinceChinaCHNAsia
119853Environment, SoilHebeiChinaCHNAsia2000

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_6969.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_103;97_336;98_2914;99_6969&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AB249956
  • Sequence Identity:
  • Total samples: 210
  • soil counts: 158
  • aquatic counts: 19
  • animal counts: 10
  • plant counts: 23

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
106201Risk group (German classification)
187241German classification
1198531Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces hebeiensis gene for 16S rRNA, partial sequence, strain: NBRC 101006AB2499561469ena229486
10620Streptomyces hebeiensis 16S ribosomal RNA gene, partial sequenceAY2775291520ena229486

GC content

@refGC-contentmethod
3006571.4
6777071.4thermal denaturation, midpoint method (Tm)

External links

@ref: 10620

culture collection no.: DSM 41837, CCTCC AA 203005, CIP 107974, NBRC 101006, YIM 001, JCM 12696, NBRC 100914

straininfo link

  • @ref: 85436
  • straininfo: 132030

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15143015Streptomyces hebeiensis sp. nov.Xu P, Li WJ, Wu WL, Wang D, Xu LH, Jiang CLInt J Syst Evol Microbiol10.1099/ijs.0.02897-02004Base Composition, Carbohydrate Metabolism, Cell Wall/chemistry, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Microscopy, Electron, Scanning, Molecular Sequence Data, Phenotype, Phospholipids/analysis, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification/metabolismMetabolism
Phylogeny19196762Streptomyces alni sp. nov., a daidzein-producing endophyte isolated from a root of Alnus nepalensis D. Don.Liu N, Wang H, Liu M, Gu Q, Zheng W, Huang YInt J Syst Evol Microbiol10.1099/ijs.0.65769-02009Alnus/*microbiology, Isoflavones/*metabolism, Molecular Sequence Data, Phylogeny, Plant Roots/microbiology, RNA, Ribosomal, 16S/genetics, Species Specificity, Streptomyces/*classification/genetics/isolation & purification/*physiologyGenetics
Phylogeny27582375Streptomyces tremellae sp. nov., isolated from a culture of the mushroom Tremella fuciformis.Wen ZQ, Chen B, Li X, Li BB, Li CH, Huang QH, Zhang QH, Dai WH, Jiang YJInt J Syst Evol Microbiol10.1099/ijsem.0.0014642016*Agaricales, Bacterial Typing Techniques, Base Composition, *Basidiomycota, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
10620Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41837)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41837
18724Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41837.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
30065Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604126425
38246Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5544
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
85436Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID132030.1StrainInfo: A central database for resolving microbial strain identifiers
119853Curators of the CIPCollection of Institut Pasteur (CIP 107974)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107974