Strain identifier

BacDive ID: 16359

Type strain: Yes

Species: Streptomyces stramineus

Strain Designation: LL-B01208

Strain history: CIP <- 1998, D.P. Labeda, Microb. Prop. Res., Peoria, IL, USA

NCBI tax ID(s): 173861 (species)

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General

@ref: 10568

BacDive-ID: 16359

DSM-Number: 41783

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped, antibiotic compound production

description: Streptomyces stramineus LL-B01208 is an obligate aerobe, mesophilic, Gram-positive bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 173861
  • Matching level: species

strain history

@refhistory
10568<- IFO <- NRRL <- D. P. Labeda
351821998, D.P. Labeda, Microb. Prop. Res., Peoria, IL, USA
67770NRRL 12292 <-- D. P. Labeda LL-BO1208.
123325CIP <- 1998, D.P. Labeda, Microb. Prop. Res., Peoria, IL, USA

doi: 10.13145/bacdive16359.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces stramineus
  • full scientific name: Streptomyces stramineus Labeda et al. 1997

@ref: 10568

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces stramineus

full scientific name: Streptomyces stramineus Labeda et al. 1997

strain designation: LL-B01208

type strain: yes

Morphology

cell morphology

  • @ref: 123325
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
18720Saffron yellow (1017)10-14 daysISP 2
18720Zinc yellow (1018)10-14 daysISP 3
18720Zinc yellow (1018)10-14 daysISP 4
18720Beige (1001)10-14 daysISP 5
18720Brown beige (1011)10-14 daysISP 6
18720Brown beige (1011)10-14 daysISP 7
123325

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
18720yesAerial MyceliumZinc yellow (1018)ISP 2
18720yesAerial MyceliumZinc yellow (1018)ISP 3
18720yesAerial MyceliumZinc yellow (1018)ISP 4
18720noISP 5
18720noISP 6
18720noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10568GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18720ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18720ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18720ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18720ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18720ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18720ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
35182MEDIUM 129 - for Pseudonocardia, Nocardia, Skermania and Streptomyces seoulensis, Streptomyces stramineusyesDistilled water make up to (1000.000 ml);Agar (20.000 g);Glucose (10.000g);Yeast extract (1.000 g);Beef extract (1.000 g);Casamino acids (2.000 g)
10568ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
10568STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
10568N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://mediadive.dsmz.de/medium/554Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
123325CIP Medium 129yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=129

culture temp

@refgrowthtypetemperaturerange
10568positivegrowth28mesophilic
18720positiveoptimum28mesophilic
35182positivegrowth30mesophilic
67770positivegrowth28mesophilic
123325positivegrowth10-41
123325nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123325
  • oxygen tolerance: obligate aerobe

compound production

@refcompound
10568bleomycin-like antibiotics
10568phleomycin
10568LL-B01208
67770Antibiotics LL-BO1208α and LL-BO1208&beta

halophily

@refsaltgrowthtested relationconcentration
123325NaClpositivegrowth0-6 %
123325NaClnogrowth8 %
123325NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis
1233254853esculin-hydrolysis
123325606565hippurate-hydrolysis
12332517632nitrate-reduction
12332516301nitrite-reduction
12332517632nitrate-respiration

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
12332535581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole-
12332515688acetoin-
12332517234glucose-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase-3.2.1.23
123325oxidase-
123325beta-galactosidase-3.2.1.23
123325alcohol dehydrogenase-1.1.1.1
123325gelatinase+
123325amylase+
123325DNase-
123325caseinase+3.4.21.50
123325catalase+1.11.1.6
123325tween esterase+
123325gamma-glutamyltransferase-2.3.2.2
123325lecithinase-
123325lipase-
123325lysine decarboxylase-4.1.1.18
123325ornithine decarboxylase-4.1.1.17
123325phenylalanine ammonia-lyase-4.3.1.24
123325tryptophan deaminase-
123325urease-3.5.1.5
68382esterase lipase (C 8)+
68382valine arylamidase-
68382trypsin+3.4.21.4
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18720+-++---++++---+-++-
123325-++-+-++--+-----+---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18720-++++-+---+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
10568soil (grassland)South DakotaUSAUSANorth America
67770SoilSDUSAUSANorth America
123325Environment, Grassland soilSouth DakotaUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Grassland
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_6264.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_91;97_97;98_1662;99_6264&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AY999928
  • Sequence Identity:
  • Total samples: 166
  • soil counts: 112
  • aquatic counts: 2
  • animal counts: 49
  • plant counts: 3

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
105681Risk group (German classification)
187201German classification
1233251Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces stramineus gene for 16S rRNA, partial sequence, strain: NBRC 16131AB1847201474ena173861
20218Streptomyces stramineus strain NRRL 12292 16S ribosomal RNA gene, partial sequenceAY9999281317ena173861

GC content

@refGC-contentmethod
1056875
6777075thermal denaturation, midpoint method (Tm)

External links

@ref: 10568

culture collection no.: DSM 41783, CIP 105398, IFO 16131, JCM 10649, NBRC 16131, NRRL 12292

straininfo link

  • @ref: 85414
  • straininfo: 50117

literature

  • topic: Phylogeny
  • Pubmed-ID: 9226907
  • title: Streptomyces stramineus sp. nov., a new species of the verticillate streptomycetes.
  • authors: Labeda DP, Lechevalier MP, Testa RT
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-47-3-747
  • year: 1997
  • mesh: DNA, Bacterial/analysis, Microscopy, Electron, Scanning, Phenotype, Streptomyces/chemistry/*classification/ultrastructure
  • topic2: Phenotype

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10568Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41783)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41783
18720Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41783.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35182Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17413
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
85414Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50117.1StrainInfo: A central database for resolving microbial strain identifiers
123325Curators of the CIPCollection of Institut Pasteur (CIP 105398)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105398