Strain identifier

BacDive ID: 16347

Type strain: Yes

Species: Streptomyces labedae

Strain Designation: Sanchez-Marroquin A-24

Strain history: NRRL B-5616 <-- ISP 5059 <-- IMRU 3737 <-- Sanchez-Marroquin A-24.

NCBI tax ID(s): 285569 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 10311

BacDive-ID: 16347

DSM-Number: 41446

keywords: spore-forming, Bacteria, 16S sequence, mesophilic

description: Streptomyces labedae Sanchez-Marroquin A-24 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 285569
  • Matching level: species

strain history

doi: 10.13145/bacdive16347.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptomycetales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces labedae
  • full scientific name: Streptomyces labedae Lacey 1987

@ref: 10311

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces labedae

full scientific name: Streptomyces labedae Lacey 1987

strain designation: Sanchez-Marroquin A-24

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19498Yellow olive10-14 daysISP 2
19498Signal brown10-14 daysISP 3
19498Grey yellow10-14 daysISP 4
19498Signal brown10-14 daysISP 5
19498Sand yellow10-14 daysISP 6
19498Signal brown10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19498yesAerial MyceliumOyster white (1013)ISP 2
19498yesAerial MyceliumOyster white (1013)ISP 3
19498yesAerial MyceliumOyster white (1013)ISP 4
19498yesAerial MyceliumOyster white (1013)ISP 5
19498yesAerial MyceliumOyster white (1013)ISP 6
19498yesAerial MyceliumOyster white (1013)ISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10311GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://bacmedia.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 12.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19498ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19498ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19498ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19498ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19498ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19498ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
10311ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://bacmedia.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
10311STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://bacmedia.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l CaCO3 1.998 g/l (NH4)2SO4 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
10311positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19498
  • spore description: Formation of spore chains (retinaculiapetri), spore surface warty
  • type of spore: spore
  • spore formation: yes

halophily

  • @ref: 19498
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1949862968cellulose+
1949816634raffinose+
1949826546rhamnose+
1949828757fructose+
1949829864mannitol+
1949817268myo-inositol+
1949818222xylose-
1949817992sucrose+
1949822599arabinose+
1949817234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19498++++++-+-++++-++++-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19498+++++-+--++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
10311soil
67770SoilMexico CityMexicoMEXNorth America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
103111Risk group (German classification)
194981Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces sp. A24 gene for 16S ribosomal RNA, partial sequenceAB8562981501ena741658
20218Streptomyces sp. A00024 16S ribosomal RNA gene, partial sequenceEF690231467ena452238
20218Streptomyces sp. A24 16S ribosomal RNA gene, partial sequenceGU4794411443ena741658
20218Streptomyces labedae strain IFO 15864 16S ribosomal RNA gene, partial sequenceAY9998681432ena285569
20218Streptomyces labedae gene for 16S rRNA, partial sequence, strain: NBRC 15864AB1847041454ena285569
20218Streptomyces labedae gene for 16S ribosomal RNA, partial sequence, strain: NBRC 15864AB9156311008ena285569

External links

@ref: 10311

culture collection no.: DSM 41446, CBS 525.64, IFO 15864, IMRU 3737, ISP 5059, JCM 9381, NBRC 15864, NRRL B-5616, CGMCC 4.1916

straininfo link

@refpassport
20218http://www.straininfo.net/strains/167025
20218http://www.straininfo.net/strains/230561
20218http://www.straininfo.net/strains/185115
20218http://www.straininfo.net/strains/753669
20218http://www.straininfo.net/strains/167026
20218http://www.straininfo.net/strains/167027
20218http://www.straininfo.net/strains/335764
20218http://www.straininfo.net/strains/167028
20218http://www.straininfo.net/strains/167029

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23355697Streptomyces wuyuanensis sp. nov., an actinomycete from soil.Zhang X, Zhang J, Zheng J, Xin D, Xin Y, Pang HInt J Syst Evol Microbiol10.1099/ijs.0.047050-02013Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/analysisGenetics
Phylogeny24306769Streptomyces tunisiensis sp. nov., a novel Streptomyces species with antibacterial activity.Slama N, Mankai H, Ayed A, Mezhoud K, Rauch C, Lazim H, Barkallah I, Gtari M, Limam FAntonie Van Leeuwenhoek10.1007/s10482-013-0086-z2013*Antibiosis, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Methicillin-Resistant Staphylococcus aureus/*growth & development, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Spores, Bacterial/cytology, Streptomyces/*classification/genetics/isolation & purification/*physiology, TunisiaGenetics
Phylogeny28174421Streptomyces euryhalinus sp. nov., a new actinomycete isolated from a mangrove forest.Biswas K, Choudhury JD, Mahansaria R, Saha M, Mukherjee JJ Antibiot (Tokyo)10.1038/ja.2017.32017DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, India, Lipids/*chemistry, Phylogeny, RNA, Ribosomal, 16S/*genetics, Random Amplified Polymorphic DNA Technique, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Streptomyces/classification/genetics/*isolation & purification, *WetlandsEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10311Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41446)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41446
19498Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41446.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym