Strain identifier

BacDive ID: 16343

Type strain: Yes

Species: Streptomyces thermocarboxydovorans

Strain Designation: AT52, AT 52

Strain history: CIP <- 1998, Seung Bum Kim, Univ. Newcastle, Dept. Agri. & Environ. Sci., United Kingdom: strain AT 52 <- C. Falconner

NCBI tax ID(s): 59298 (species)

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General

@ref: 11653

BacDive-ID: 16343

DSM-Number: 44296

keywords: 16S sequence, Bacteria, spore-forming, thermophilic, Gram-positive, rod-shaped

description: Streptomyces thermocarboxydovorans AT52 is a spore-forming, thermophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from allotment garden soil.

NCBI tax id

  • NCBI tax id: 59298
  • Matching level: species

strain history

@refhistory
11653<- S.B. Kim (M. Goodfellow), AT52
67770S. B. Kim AT52 <-- C. Falconer.
123503CIP <- 1998, Seung Bum Kim, Univ. Newcastle, Dept. Agri. & Environ. Sci., United Kingdom: strain AT 52 <- C. Falconner

doi: 10.13145/bacdive16343.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces thermocarboxydovorans
  • full scientific name: Streptomyces thermocarboxydovorans Kim et al. 1998

@ref: 11653

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces thermocarboxydovorans

full scientific name: Streptomyces thermocarboxydovorans Kim et al. 1998

strain designation: AT52, AT 52

type strain: yes

Morphology

cell morphology

  • @ref: 123503
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
20043Dahlia yellow (1033)10-14 daysISP 2
20043Colorless10-14 daysISP 3
20043Colorless10-14 daysISP 4
20043Colorless10-14 daysISP 5
20043Colorless10-14 daysISP 6
20043Colorless10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
20043yesAerial MyceliumWhiteISP 2
20043yesAerial MyceliumWhiteISP 3
20043yesAerial MyceliumWhiteISP 4
20043yesAerial MyceliumWhiteISP 5
20043noISP 6
20043yesAerial MyceliumWhiteISP 7

multimedia

  • @ref: 11653
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44296.jpg
  • caption: Medium 248 45°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11653STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
20043ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20043ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20043ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20043ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20043ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20043ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
38562MEDIUM 129 - for Pseudonocardia, Nocardia, Skermania and Streptomyces seoulensis, Streptomyces stramineusyesDistilled water make up to (1000.000 ml);Agar (20.000 g);Glucose (10.000g);Yeast extract (1.000 g);Beef extract (1.000 g);Casamino acids (2.000 g)
11653OAT FLAKES AGAR WITH YEAST EXTRACT (DSMZ Medium 248)yeshttps://mediadive.dsmz.de/medium/248Name: OAT FLAKES AGAR WITH YEAST EXTRACT (DSMZ Medium 248) Composition: Agar 20.0 g/l Oat flakes 20.0 g/l Yeast extract 2.0 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water
11653BENNETT'S MODIFIED MEDIUM (P. AGRAWAL, UNPUBLISHED) (DSMZ Medium 894)yeshttps://mediadive.dsmz.de/medium/894Name: BENNETT'S MODIFIED MEDIUM (P. AGRAWAL, UNPUBLISHED) (DSMZ Medium 894) Composition: Agar 15.0 g/l Lab-Lemco beef extract 10.0 g/l Glucose 10.0 g/l Tryptose 2.0 g/l Yeast extract 2.0 g/l CaCO3 0.1 g/l Starch 0.1 g/l CoCl2 Ferric ammonium citrate Distilled water
123503CIP Medium 129yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=129

culture temp

@refgrowthtypetemperaturerange
11653positivegrowth45thermophilic
20043positiveoptimum45thermophilic
38562positivegrowth45thermophilic
67770positivegrowth45thermophilic
123503positivegrowth25-45
123503nogrowth10psychrophilic

Physiology and metabolism

spore formation

  • @ref: 20043
  • spore description: Formation of spore chains (rectiflixibilis), spore surface smooth
  • spore formation: yes

halophily

  • @ref: 20043
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2004317234glucose-
2004322599arabinose-
2004317992sucrose-
2004318222xylose-
2004317268myo-inositol-
2004329864mannitol+
2004328757fructose+
2004326546rhamnose-
2004316634raffinose-
2004362968cellulose-
1235034853esculin-hydrolysis
123503606565hippurate-hydrolysis
12350317632nitrate+reduction
12350316301nitrite-reduction

metabolite production

  • @ref: 123503
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12350315688acetoin-
12350317234glucose-

enzymes

@refvalueactivityec
123503oxidase-
123503beta-galactosidase+3.2.1.23
123503alcohol dehydrogenase-1.1.1.1
123503gelatinase+/-
123503amylase+
123503DNase+
123503caseinase+3.4.21.50
123503catalase+1.11.1.6
123503tween esterase+
123503gamma-glutamyltransferase+2.3.2.2
123503lecithinase-
123503lipase-
123503lysine decarboxylase-4.1.1.18
123503ornithine decarboxylase-4.1.1.17
123503phenylalanine ammonia-lyase-4.3.1.24
123503protease-
123503tryptophan deaminase-
123503urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123503-+++++++-+++-+---+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11653allotment garden soilNewcastle upon TyneUnited KingdomGBREurope
67770Allotment garden soilUnited KingdomGBREurope
123503Environment, Allotment soilUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Garden
#Environmental#Terrestrial#Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
116531Risk group (German classification)
200431German classification
1235031Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
11653Streptomyces thermocarboxydovorans DSM 44296 16S ribosomal RNA gene, complete sequenceU944891502ena59298
11653Streptomyces thermocarboxydovorans gene for 16S rRNA, partial sequence, strain: NBRC 16324AB2499291484ena59298

GC content

@refGC-contentmethod
1165374.7
6777074.7high performance liquid chromatography (HPLC)

External links

@ref: 11653

culture collection no.: DSM 44296, IFO 16324, NBRC 16324, JCM 10367, CIP 105544, KCTC 9938, NCIMB 13530, NRRL B-24317

straininfo link

  • @ref: 85398
  • straininfo: 50555

Reference

@idauthorscataloguedoi/urltitle
11653Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44296)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44296
20043Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44296.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
38562Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17575
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
85398Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50555.1StrainInfo: A central database for resolving microbial strain identifiers
123503Curators of the CIPCollection of Institut Pasteur (CIP 105544)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105544