Strain identifier

BacDive ID: 16340

Type strain: Yes

Species: Streptomyces thermocarboxydus

Strain Designation: AT37

Strain history: CIP <- 1998, Seung Bum Kim, Univ. Newcastle, Dept. Agri. & Environ. Sci., United Kingdom: strain AT37 <- C. Falconer

NCBI tax ID(s): 59299 (species)

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General

@ref: 11650

BacDive-ID: 16340

DSM-Number: 44293

keywords: 16S sequence, Bacteria, obligate aerobe, spore-forming, thermophilic, Gram-positive, rod-shaped

description: Streptomyces thermocarboxydus AT37 is an obligate aerobe, spore-forming, thermophilic bacterium that builds an aerial mycelium and was isolated from allotment garden soil.

NCBI tax id

  • NCBI tax id: 59299
  • Matching level: species

strain history

@refhistory
11650<- S.B. Kim (M. Goodfellow), AT37
67770S. B. Kim AT37 <-- C. Falconer.
123504CIP <- 1998, Seung Bum Kim, Univ. Newcastle, Dept. Agri. & Environ. Sci., United Kingdom: strain AT37 <- C. Falconer

doi: 10.13145/bacdive16340.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces thermocarboxydus
  • full scientific name: Streptomyces thermocarboxydus Kim et al. 1998

@ref: 11650

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces thermocarboxydus

full scientific name: Streptomyces thermocarboxydus Kim et al. 1998

strain designation: AT37

type strain: yes

Morphology

cell morphology

  • @ref: 123504
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
19648Black grey10-14 daysISP 2
19648Beige10-14 daysISP 3
1964810-14 daysISP 4
1964810-14 daysISP 5
1964810-14 daysISP 6
1964810-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19648yesAerial MyceliumPlatinum greyISP 2
19648yesAerial MyceliumISP 3
19648yesAerial MyceliumSignal greyISP 4
19648yesAerial MyceliumWhiteISP 5
19648yesAerial MyceliumWhiteISP 6
19648yesAerial MyceliumISP 7

multimedia

  • @ref: 11650
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44293.jpg
  • caption: Medium 65 45°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11650ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
11650GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
32753MEDIUM 129 - for Pseudonocardia, Nocardia, Skermania and Streptomyces seoulensis, Streptomyces stramineusyesDistilled water make up to (1000.000 ml);Agar (20.000 g);Glucose (10.000g);Yeast extract (1.000 g);Beef extract (1.000 g);Casamino acids (2.000 g)
19648ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19648ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19648ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19648ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19648ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19648ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
123504CIP Medium 129yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=129

culture temp

@refgrowthtypetemperaturerange
11650positivegrowth45thermophilic
19648positiveoptimum45thermophilic
32753positivegrowth45thermophilic
67770positivegrowth45thermophilic
123504positivegrowth25-45
123504nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123504
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 19648
  • spore description: Formation of spore chains (retinaculiapetri), spore surface warty
  • type of spore: spore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
19648NaClpositivemaximum5 %
123504NaClpositivegrowth0-4 %
123504NaClnogrowth6 %
123504NaClnogrowth8 %
123504NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1964817234glucose+
1964822599arabinose+
1964817992sucrose+
1964818222xylose-
1964817268myo-inositol-
1964829864mannitol-
1964828757fructose-
1964826546rhamnose-
1964816634raffinose-
1964862968cellulose-
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose+builds acid from
6837116443D-tagatose+builds acid from
6837162318D-lyxose+builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose+builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
1235044853esculin-hydrolysis
123504606565hippurate-hydrolysis
12350417632nitrate+reduction
12350416301nitrite-reduction
12350417632nitrate-respiration

metabolite production

  • @ref: 123504
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12350415688acetoin-
12350417234glucose-

enzymes

@refvalueactivityec
123504oxidase-
123504beta-galactosidase+3.2.1.23
123504alcohol dehydrogenase-1.1.1.1
123504gelatinase+/-
123504amylase+
123504caseinase-3.4.21.50
123504catalase+1.11.1.6
123504tween esterase-
123504gamma-glutamyltransferase-2.3.2.2
123504lysine decarboxylase-4.1.1.18
123504ornithine decarboxylase-4.1.1.17
123504phenylalanine ammonia-lyase-4.3.1.24
123504tryptophan deaminase-
123504urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123504-+---+-----+-----+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123504----+--------+-------------------------++++-----+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123504+++++----+++++-+++++++++--+----+++++-----+------++----+---+++++-+-------++-+-++++---+-+-++++-++++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11650allotment garden soilNewcastle upon TyneUnited KingdomGBREurope
67770Allotment garden soilUnited KingdomGBREurope
123504Environment, Allotment soilUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Garden
#Environmental#Terrestrial#Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
116501Risk group (German classification)
196481Risk group (German classification)
1235041Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces thermocarboxydus DSM 44293 16S ribosomal RNA gene, complete sequenceU944901500ena59299
20218Streptomyces thermocarboxydus gene for 16S rRNA, partial sequence, strain: NBRC 16323AB2499261453ena59299

GC content

@refGC-contentmethod
1165070.9
6777070.5-71.3high performance liquid chromatography (HPLC)

External links

@ref: 11650

culture collection no.: DSM 44293, IFO 16323, NBRC 16323, JCM 10368, CGMCC 4.1883, CIP 105545, KCTC 9937, NCIMB 13531, NRRL B-24316, VTT E-011979

straininfo link

  • @ref: 85395
  • straininfo: 50552

literature

  • topic: Phylogeny
  • Pubmed-ID: 25672321
  • title: Isolation and characterization of Cr(VI)-reducing actinomycetes from estuarine sediments.
  • authors: Terahara T, Xu X, Kobayashi T, Imada C
  • journal: Appl Biochem Biotechnol
  • DOI: 10.1007/s12010-015-1501-x
  • year: 2015
  • mesh: Actinobacteria/classification/*genetics/*isolation & purification/metabolism, *Biodegradation, Environmental, Chromium/*metabolism/toxicity, Geologic Sediments/microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Soil Microbiology, Soil Pollutants/metabolism/toxicity
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11650Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44293)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44293
19648Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44293.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32753Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17576
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
85395Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50552.1StrainInfo: A central database for resolving microbial strain identifiers
123504Curators of the CIPCollection of Institut Pasteur (CIP 105545)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105545