Strain identifier

BacDive ID: 16338

Type strain: Yes

Species: Streptomyces violaceoruber

Strain Designation: FH 2456

Strain history: KCC S-1065 <-- MS 1481 <-- CBS 103.21 <-- H. W. Wollenweber.

NCBI tax ID(s): 68277 (species)

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General

@ref: 10484

BacDive-ID: 16338

DSM-Number: 41704

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces violaceoruber FH 2456 is a spore-forming, mesophilic bacterium that builds an aerial mycelium.

NCBI tax id

  • NCBI tax id: 68277
  • Matching level: species

strain history

@refhistory
10484<- Hoechst AG, FH 2456 <- CBS
67770KCC S-1065 <-- MS 1481 <-- CBS 103.21 <-- H. W. Wollenweber.

doi: 10.13145/bacdive16338.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces violaceoruber
  • full scientific name: Streptomyces violaceoruber (Waksman and Curtis 1916) Pridham 1970 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Actinopycnidium caeruleum
    20215Actinomyces tricolor
    20215Actinomyces anthocyanicus
    20215Actinomyces violaceolatus
    20215Streptomyces humiferus
    20215Streptomyces anthocyanicus
    20215Streptomyces tricolor
    20215Streptomyces violaceolatus
    20215Streptomyces roseodiastaticus
    20215Actinomyces violaceusruber
    20215Actinomyces coelescens
    20215Actinomyces roseodiastaticus
    20215Streptomyces coelescens

@ref: 10484

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces tricolor

full scientific name: Streptomyces tricolor (Wollenweber 1920) Waksman 1961 emend. Lanoot et al. 2004

strain designation: FH 2456

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19529Black10-14 daysISP 2
19529Red10-14 daysISP 3
19529Red10-14 daysISP 4
19529Red10-14 daysISP 5
1952910-14 daysISP 6
1952910-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19529yesAerial MyceliumWhiteISP 2
19529yesAerial MyceliumGreyISP 3
19529yesAerial MyceliumWhiteISP 4
19529yesAerial MyceliumGreyISP 5
19529noISP 6
19529yesAerial MyceliumGreyISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10484GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19529ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19529ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19529ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19529ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19529ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19529ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
10484STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
10484ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
10484positivegrowth28mesophilic
19529positiveoptimum28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19529
  • spore description: Formation of spore chains: spirales, spore surface: smooth
  • type of spore: spore
  • spore formation: yes

halophily

  • @ref: 19529
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1952917234glucose+
1952922599arabinose-
1952917992sucrose-
1952918222xylose-
1952917268myo-inositol-
1952929864mannitol-
1952928757fructose-
1952926546rhamnose-
1952916634raffinose-
1952962968cellulose-
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase+4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19529++-----+--+

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
104841Risk group (German classification)
195291Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces tricolor gene for 16S ribosomal RNA, partial sequence, strain: JCM 5065D44434121ena68277
20218Streptomyces tricolor 16S rRNA gene, type strain LMG 20328AJ7813801442ena68277
20218Streptomyces tricolor gene for 16S rRNA, partial sequence, strain: NBRC 15461AB1846871450ena68277

Genome sequences

  • @ref: 67770
  • description: Streptomyces tricolor NRRL B-16925
  • accession: GCA_002155905
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 68277

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno97.941no
gram-positiveyes89.339no
anaerobicno99.211no
halophileno94.025yes
spore-formingyes94.798yes
glucose-utilyes90.168yes
aerobicyes93.707no
thermophileno97.706no
motileno93.067no
glucose-fermentno91.847no

External links

@ref: 10484

culture collection no.: DSM 41704, CBS 103.21, IFO 15461, JCM 5065, KCC S-1065, NBRC 15461, NRRL B-16925, NBRC 103112

straininfo link

  • @ref: 85393
  • straininfo: 50058

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10484Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41704)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41704
19529Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41704.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
85393Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50058.1StrainInfo: A central database for resolving microbial strain identifiers