Strain identifier
BacDive ID: 16327
Type strain:
Species: Streptomyces laurentii
Strain history: KCC S-1063 <-- MS 1477 <-- ATCC 31255 <-- SC 9895.
NCBI tax ID(s): 39478 (species)
General
@ref: 10467
BacDive-ID: 16327
DSM-Number: 41684
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic
description: Streptomyces laurentii DSM 41684 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.
NCBI tax id
- NCBI tax id: 39478
- Matching level: species
strain history
@ref | history |
---|---|
10467 | <- ATCC <- E. R. Squibb and Sons Inc., SC9895 |
67770 | KCC S-1063 <-- MS 1477 <-- ATCC 31255 <-- SC 9895. |
doi: 10.13145/bacdive16327.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces laurentii
- full scientific name: Streptomyces laurentii Trejo et al. 1979 (Approved Lists 1980)
@ref: 10467
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces laurentii
full scientific name: Streptomyces laurentii Trejo et al. 1979 emend. Nouioui et al. 2018
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | no | 91.615 | |
69480 | 100 | positive |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19523 | Red | 10-14 days | ISP 2 |
19523 | Rose | 10-14 days | ISP 3 |
19523 | Red | 10-14 days | ISP 4 |
19523 | Rose | 10-14 days | ISP 5 |
19523 | Brown | 10-14 days | ISP 6 |
19523 | Red | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
19523 | yes | Aerial Mycelium | Rose | ISP 2 |
19523 | yes | Aerial Mycelium | Rose | ISP 3 |
19523 | yes | Aerial Mycelium | Rose | ISP 4 |
19523 | yes | Aerial Mycelium | Rose | ISP 5 |
19523 | no | ISP 6 | ||
19523 | yes | Aerial Mycelium | Rose | ISP 7 |
multimedia
- @ref: 10467
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_41684.jpg
- caption: Medium 65 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
10467 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
19523 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
19523 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
19523 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
19523 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
19523 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
19523 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
10467 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://mediadive.dsmz.de/medium/84 | Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water |
10467 | STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) | yes | https://mediadive.dsmz.de/medium/252 | Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
10467 | positive | growth | 28 | mesophilic |
19523 | positive | optimum | 26 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
@ref | spore description | type of spore | spore formation | confidence |
---|---|---|---|---|
19523 | Formation of spore chains: rectiflixibilis, spore surface: smooth | spore | yes | |
69481 | yes | 100 | ||
69480 | yes | 100 |
compound production
- @ref: 10467
- compound: thiostrepton
halophily
- @ref: 19523
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 2.5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
19523 | 62968 | cellulose | - | |
19523 | 16634 | raffinose | - | |
19523 | 26546 | rhamnose | - | |
19523 | 28757 | fructose | +/- | |
19523 | 29864 | mannitol | - | |
19523 | 17268 | myo-inositol | +/- | |
19523 | 18222 | xylose | + | |
19523 | 17992 | sucrose | +/- | |
19523 | 22599 | arabinose | - | |
19523 | 17234 | glucose | + | |
68368 | 5291 | gelatin | + | hydrolysis |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | + | hydrolysis |
68368 | 16947 | citrate | + | assimilation |
68368 | 18257 | ornithine | + | degradation |
68368 | 25094 | lysine | + | degradation |
68368 | 29016 | arginine | + | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
67770 | 29693 | thiostrepton | yes |
68368 | 15688 | acetoin | yes |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | + | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68368 | gelatinase | + | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | + | 3.5.1.5 |
68368 | ornithine decarboxylase | + | 4.1.1.17 |
68368 | lysine decarboxylase | + | 4.1.1.18 |
68368 | arginine dihydrolase | + | 3.5.3.6 |
68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
---|---|---|---|---|---|---|---|---|---|---|---|
19523 | + | + | + | + | + | - | + | - | - | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
10467 | soil |
67770 | Soil |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_17744.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_109;98_766;99_17744&stattab=map
- Last taxonomy: Streptomyces
- 16S sequence: AB184669
- Sequence Identity:
- Total samples: 1989
- soil counts: 1114
- aquatic counts: 48
- animal counts: 54
- plant counts: 773
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
10467 | 1 | Risk group (German classification) |
19523 | 1 | Risk group (German classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Streptomyces laurentii gene for 16S ribosomal RNA, partial sequence, strain: JCM 5063 | D44432 | 120 | ena | 39478 |
20218 | Streptomyces laurentii 16S rRNA gene, type strain LMG 19959 | AJ781342 | 1478 | ena | 39478 |
20218 | Streptomyces laurentii gene for 16S rRNA, partial sequence, strain: NBRC 15422 | AB184669 | 1472 | ena | 39478 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Streptomyces laurentii ATCC 31255 | GCA_002355495 | complete | ncbi | 39478 |
66792 | Streptomyces laurentii strain ATCC 31255 | 39478.5 | plasmid | patric | 39478 |
66792 | Streptomyces laurentii strain ATCC 31255 | 39478.4 | complete | patric | 39478 |
66792 | Streptomyces laurentii ATCC 31255 | 2563366701 | draft | img | 39478 |
66792 | Streptomyces laurentii ATCC 31255 | 2563366700 | draft | img | 39478 |
GC content
- @ref: 67770
- GC-content: 72.3
- method: genome sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
flagellated | no | 97.476 | no |
gram-positive | yes | 89.076 | no |
anaerobic | no | 98.773 | no |
aerobic | yes | 87.774 | no |
halophile | no | 96.155 | no |
spore-forming | yes | 93.458 | yes |
glucose-util | yes | 90.921 | yes |
thermophile | no | 97.217 | yes |
motile | no | 94.101 | no |
glucose-ferment | no | 85.988 | no |
External links
@ref: 10467
culture collection no.: DSM 41684, ATCC 31255, JCM 5063, KCC S-1063, PCM 2368, BCRC 16804, CGMCC 4.1919, IFO 15422, NBRC 15422
straininfo link
- @ref: 85383
- straininfo: 39963
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Enzymology | 9339547 | A conjugative linear plasmid in Streptomyces laurentii ATCC31255. | Kinoshita-Iramina C, Kitahara M, Doi K, Ogata S | Biosci Biotechnol Biochem | 10.1271/bbb.61.1469 | 1997 | Anti-Bacterial Agents/biosynthesis, Blotting, Southern, Conjugation, Genetic/*genetics, Culture Media, Electrophoresis, Polyacrylamide Gel, Exonucleases/metabolism, Plasmids/*genetics, Restriction Mapping, Spores, Bacterial/genetics, Streptomyces/enzymology/*genetics/growth & development, Thiostrepton/biosynthesis | Genetics |
Biotechnology | 21107477 | Heterologous production of thiostrepton A and biosynthetic engineering of thiostrepton analogs. | Li C, Zhang F, Kelly WL | Mol Biosyst | 10.1039/c0mb00129e | 2010 | Anti-Bacterial Agents/*biosynthesis, Biotechnology/*methods, Cloning, Molecular, Multigene Family/genetics/physiology, Streptomyces/genetics/*metabolism, Thiostrepton/*analogs & derivatives/*biosynthesis | Enzymology |
Genetics | 27257211 | Complete Genome Sequence of Thiostrepton-Producing Streptomyces laurentii ATCC 31255. | Doi K, Fujino Y, Nagayoshi Y, Ohshima T, Ogata S | Genome Announc | 10.1128/genomeA.00360-16 | 2016 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
10467 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41684) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-41684 | |||
19523 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM41684.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
85383 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID39963.1 | StrainInfo: A central database for resolving microbial strain identifiers |