Strain identifier

BacDive ID: 16301

Type strain: Yes

Species: Streptomyces carpinensis

Strain Designation: 70-6-2, M 619

Strain history: KCC A-0301 <-- S. T. Williams M 619 <-- J. O. Falcão de Morais 70-6-2.

NCBI tax ID(s): 66369 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 11319

BacDive-ID: 16301

DSM-Number: 43835

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces carpinensis 70-6-2 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 66369
  • Matching level: species

strain history

@refhistory
11319<- KCC <- S. T. Williams, M 619 <- J. O. Falcao de Morais, 70-6-2
67770KCC A-0301 <-- S. T. Williams M 619 <-- J. O. Falcão de Morais 70-6-2.

doi: 10.13145/bacdive16301.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces carpinensis
  • full scientific name: Streptomyces carpinensis (Falcão de Morais et al. 1971) Goodfellow et al. 1986
  • synonyms

    • @ref: 20215
    • synonym: Elytrosporangium carpinense

@ref: 11319

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces carpinensis

full scientific name: Streptomyces carpinensis (Falcão de Morais et al. 1971) Goodfellow et al. 1986

strain designation: 70-6-2, M 619

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19962Beige (1001)10-14 daysISP 2
19962Black10-14 daysISP 3
19962Yellow10-14 daysISP 4
19962Green10-14 daysISP 5
19962Black10-14 daysISP 6
19962Beige10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19962yesAerial MyceliumGreyISP 2
19962yesAerial MyceliumGreyISP 3
19962yesAerial MyceliumGreyISP 4
19962yesAerial MyceliumGreyISP 5
19962yesAerial MyceliumWhiteISP 6
19962yesAerial MyceliumWhiteISP 7

multimedia

  • @ref: 11319
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43835.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11319GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19962ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19962ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19962ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19962ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19962ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19962ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
11319GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
11319positivegrowth28mesophilic
19962positiveoptimum28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19962
  • spore description: Formation of spore chains (rectiflixibilis), spore surface smooth
  • spore formation: yes

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1996262968cellulose-
1996216634raffinose-
1996226546rhamnose-
1996228757fructose+
1996229864mannitol+
1996217268myo-inositol-
1996218222xylose-
1996217992sucrose-
1996222599arabinose+
1996217234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate-assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19962++++--+--++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11319soilCarpina, PernambucoBrazilBRAMiddle and South America
67770SoilCarpina, PernambucoBrazilBRAMiddle and South America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_5549.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_91;97_287;98_384;99_5549&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AY999908
  • Sequence Identity:
  • Total samples: 710
  • soil counts: 642
  • aquatic counts: 29
  • animal counts: 35
  • plant counts: 4

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
113191Risk group (German classification)
199621German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces carpinensis gene for 16S rRNA, partial sequenceAB122728564ena66369
20218Streptomyces carpinensis gene for 16S ribosomal RNA, partial sequence, strain: JCM 3301D43982120ena66369
20218Streptomyces carpinensis gene for 16S rRNA, partial sequence, strain: NBRC 14214AB1845741476ena66369
20218Streptomyces carpinensis strain NRRL B-16921 16S ribosomal RNA gene, partial sequenceAY9999081285ena66369
20218Streptomyces carpinensis strain NRRL B-16921 16S ribosomal RNA gene, partial sequenceDQ0266641327ena66369

Genome sequences

  • @ref: 67770
  • description: Streptomyces carpinensis NRRL B-16921
  • accession: GCA_002154505
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 66369

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno98.259no
gram-positiveyes87.427no
anaerobicno98.994no
halophileno93.994no
spore-formingyes93.677yes
glucose-utilyes89.457yes
aerobicyes94.102no
thermophileno98.405yes
motileno93.816no
glucose-fermentno90.353no

External links

@ref: 11319

culture collection no.: DSM 43835, ATCC 27116, IFO 14214, IMET 43558, JCM 3301, KCC A-0301, NBRC 14214, NRRL B-16921, RIA 982, VKM Ac-1300, VKM Ac-657, BCRC 16854, CGMCC 4.1947, KCTC 9128

straininfo link

  • @ref: 85358
  • straininfo: 38819

literature

  • topic: Phylogeny
  • Pubmed-ID: 33415367
  • title: Streptomyces cupreus sp. nov., an antimicrobial producing actinobacterium isolated from Himalayan soil.
  • authors: Maiti PK, Mandal S
  • journal: Arch Microbiol
  • DOI: 10.1007/s00203-020-02160-y
  • year: 2021
  • mesh: DNA, Bacterial/genetics, India, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Species Specificity, *Streptomyces/classification/genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11319Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43835)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43835
19962Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43835.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
85358Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38819.1StrainInfo: A central database for resolving microbial strain identifiers