Strain identifier

BacDive ID: 16301

Type strain: Yes

Species: Streptomyces carpinensis

Strain Designation: 70-6-2, M 619

Culture col. no.: DSM 43835, ATCC 27116, IFO 14214, IMET 43558, JCM 3301, KCC A-0301, NBRC 14214, NRRL B-16921, RIA 982, VKM Ac-1300, VKM Ac-657

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General

@ref: 11319

BacDive-ID: 16301

DSM-Number: 43835

keywords: spore-forming, Bacteria, 16S sequence, genome sequence, mesophilic

description: Streptomyces carpinensis 70-6-2 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

strain history: <- KCC <- S. T. Williams, M 619 <- J. O. Falcao de Morais, 70-6-2

doi: 10.13145/bacdive16301.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Streptomycetales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces carpinensis
  • full scientific name: Streptomyces carpinensis (Falcao de Morais et al. 1971) Goodfellow et al. 1986
  • synonyms

    • @ref: 20215
    • synonym: Elytrosporangium carpinense

@ref: 11319

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces carpinensis

full scientific name: Streptomyces carpinensis (Falcão de Morais et al. 1971) Goodfellow et al. 1986

strain designation: 70-6-2, M 619

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19962Beige (1001)10-14 daysISP 2
19962Black10-14 daysISP 3
19962Yellow10-14 daysISP 4
19962Green10-14 daysISP 5
19962Black10-14 daysISP 6
19962Beige10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19962yesAerial MyceliumGreyISP 2
19962yesAerial MyceliumGreyISP 3
19962yesAerial MyceliumGreyISP 4
19962yesAerial MyceliumGreyISP 5
19962yesAerial MyceliumWhiteISP 6
19962yesAerial MyceliumWhiteISP 7

multimedia

  • @ref: 11319
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43835.jpg
  • caption: Medium 65 28°C
  • license or copyright: (C) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11319GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
19962ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19962ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19962ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19962ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19962ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19962ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
11319positivegrowth28mesophilic
19962positiveoptimum28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19962
  • spore description: Formation of spore chains (rectiflixibilis), spore surface smooth
  • spore formation: yes

metabolite utilization

@refChebi-IDmetaboliteutilization activity
1996262968cellulose-
1996216634raffinose-
1996226546rhamnose-
1996228757fructose+
1996229864mannitol+
1996217268myo-inositol-
1996218222xylose-
1996217992sucrose-
1996222599arabinose+
1996217234glucose+

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19962++++--+--++

Isolation, sampling and environmental information

isolation

  • @ref: 11319
  • sample type: soil
  • geographic location: Carpina, Pernambuco
  • country: Brazil
  • origin.country: BRA
  • continent: Middle and South America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
113191Risk group (German classification)
199621German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces carpinensis gene for 16S rRNA, partial sequenceAB122728564ena66369
20218Streptomyces carpinensis gene for 16S ribosomal RNA, partial sequence, strain: JCM 3301D43982120ena66369
20218Streptomyces carpinensis gene for 16S rRNA, partial sequence, strain: NBRC 14214AB1845741476ena66369
20218Streptomyces carpinensis strain NRRL B-16921 16S ribosomal RNA gene, partial sequenceAY9999081285ena66369
20218Streptomyces carpinensis strain NRRL B-16921 16S ribosomal RNA gene, partial sequenceDQ0266641327ena66369

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces carpinensis NRRL B-16921GCA_002154505scaffoldncbi66369
66792Streptomyces carpinensis strain NRRL B-1692166369.3wgspatric66369

External links

@ref: 11319

culture collection no.: DSM 43835, ATCC 27116, IFO 14214, IMET 43558, JCM 3301, KCC A-0301, NBRC 14214, NRRL B-16921, RIA 982, VKM Ac-1300, VKM Ac-657

straininfo link

@refpassport
20218http://www.straininfo.net/strains/168811
20218http://www.straininfo.net/strains/104931
20218http://www.straininfo.net/strains/104932
20218http://www.straininfo.net/strains/168812
20218http://www.straininfo.net/strains/168813
20218http://www.straininfo.net/strains/168814
20218http://www.straininfo.net/strains/333705
20218http://www.straininfo.net/strains/168815
20218http://www.straininfo.net/strains/168816
20218http://www.straininfo.net/strains/168817
20218http://www.straininfo.net/strains/168818

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11319Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43835)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43835
19962Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43835.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)