Strain identifier

BacDive ID: 162366

Type strain: Yes

Species: Haloarcula sebkhae

Strain Designation: SWO25

Strain history: A. Carre-Mlouka; CNRS-MNHN, France; SWO25 <-- N. Imadalou-Idres; Univ. of Bejaia, Algeria.

NCBI tax ID(s): 932660 (species)

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General

@ref: 67770

BacDive-ID: 162366

keywords: genome sequence, 16S sequence, Archaea, aerobe, mesophilic, Gram-negative, coccus-shaped, colony-forming

description: Haloarcula sebkhae SWO25 is an aerobe, mesophilic, Gram-negative archaeon that forms circular colonies and was isolated from Salt lake of Ouargla.

NCBI tax id

  • NCBI tax id: 932660
  • Matching level: species

strain history

  • @ref: 67770
  • history: A. Carre-Mlouka; CNRS-MNHN, France; SWO25 <-- N. Imadalou-Idres; Univ. of Bejaia, Algeria.

doi: 10.13145/bacdive162366.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Halobacteria
  • order: Halobacteriales
  • family: Haloarculaceae
  • genus: Haloarcula
  • species: Haloarcula sebkhae
  • full scientific name: Haloarcula sebkhae Barreteau et al. 2019

@ref: 67770

domain: Archaea

phylum: Euryarchaeota

class: Halobacteria

order: Halobacteriales

family: Haloarculaceae

genus: Haloarcula

species: Haloarcula sebkhae

full scientific name: Haloarcula sebkhae Barreteau et al. 2019

strain designation: SWO25

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotilityconfidence
67819negative1-3 µmcoccus-shapedno
67819rod-shaped
125439negative96.5

colony morphology

  • @ref: 67819
  • colony size: 2-6 mm
  • colony color: red
  • colony shape: circular
  • incubation period: 7-14 days
  • medium used: Halobacteria DSMZ medium 372

Culture and growth conditions

culture temp

@refgrowthtypetemperature
67770positivegrowth37
67819positivegrowth25-55
67819positiveoptimum35-45

culture pH

@refabilitytypepHPH range
67819positivegrowth5-9alkaliphile
67819positiveoptimum7-8

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
67819aerobe
125439aerobe91.3

spore formation

  • @ref: 125439
  • spore formation: no
  • confidence: 95.3

halophily

@refsaltgrowthtested relationconcentration
67819NaClpositivegrowth1.7-5.1 M
67819NaClpositiveoptimum4.3-5.1 M

observation

@refobservation
67770quinones: MK-8
67819optimum growth occurs with 0.05-0.25 M Mg2+.
67819Anaerobic growth was observed in the presence of nitrate and DMSO, but not in the presence of L-arginine.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6781918420magnesium(2+)-required for growth
6781917632nitrate+reduction
6781917632nitrate+builds acid from
678195291gelatin+hydrolysis
6781928017starch+hydrolysis
6781953426tween 80+hydrolysis
67819casein-hydrolysis
6781916551D-trehalose+carbon source
6781917057cellobiose+carbon source
6781917992sucrose+carbon source
6781917925alpha-D-glucose+carbon source
6781915824D-fructose+carbon source
6781912936D-galactose+carbon source
6781917924D-sorbitol+carbon source
6781917754glycerol+carbon source
6781916634raffinose-carbon source
6781936219alpha-lactose-carbon source
6781916024D-mannose-carbon source
6781929991L-aspartate-carbon source
6781918019L-lysine+carbon source
6781965327D-xylose+carbon source
6781916988D-ribose+carbon source
6781965327D-xylose+builds acid from
6781916988D-ribose+builds acid from
6781917634D-glucose+builds acid from
6781912936D-galactose+builds acid from
6781917924D-sorbitol+builds acid from
6781917754glycerol+builds acid from
6781927082trehalose-builds acid from
6781915824D-fructose-builds acid from
6781917992sucrose-builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6781928368novobiocinyesyes5 µg (disc)
67819338412(-)-anisomycinyesyes50 µg (disc)
6781928971ampicillinyesyes30 µg (disc)
6781928669bacitracinyesyes10 µg (disc)
6781948923erythromycinyesyes15 µg (disc)
678197507neomycinyesyes30 µg (disc)
6781917698chloramphenicolyesyes30 µg (disc)
6781928077rifampicinyesyes5 µg (disc)

metabolite production

  • @ref: 67819
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
67819catalase+1.11.1.6
67819cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic locationenrichment cultureenrichment culture compositionenrichment culture temperature
67770Salt lake of OuarglaAlgeriaDZAAfrica
67819salt water sample (pH 8.1, 4.8 M NaCl)AlgeriaDZAAfrica1997sebkha of Ouargla in the Saharaisolation medium1 % (w/v) peptone (Merck), 0.1 % (w/v) meat extract, 0.2 % (w/v) yeast extract, 4.3 M NaCl, pH 7.2. The medium was solidified with 2 % agar.37

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
67770Haloarcula sebkhae from Algeria 16S ribosomal RNA (rrnB) gene, partial sequenceHQ6417471441nuccore932660
67819Haloarcula sebkhae from Algeria 16S ribosomal RNA (rrnA) gene, partial sequenceHQ8445271441nuccore932660

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Haloarcula sebkhae JCM 19018GCA_014647415scaffoldncbi932660
66792Haloarcula sebkhae strain JCM 19018932660.3wgspatric932660

GC content

@refGC-contentmethod
6777066.1high performance liquid chromatography (HPLC)
6781961.1high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno78.006yes
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no85.468yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes78.023no
125438spore-formingspore-formingAbility to form endo- or exosporesno80.536no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno83.121yes
125438motile2+flagellatedAbility to perform flagellated movementno85.029yes
125439BacteriaNetspore_formationAbility to form endo- or exosporesno95.3
125439BacteriaNetmotilityAbility to perform movementyes54.2
125439BacteriaNetgram_stainReaction to gram-stainingnegative96.5
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthaerobe91.3

External links

@ref: 67770

culture collection no.: JCM 19018, CIP 110583

literature

  • topic: Phylogeny
  • Pubmed-ID: 30628879
  • title: Haloarcula sebkhae sp. nov., an extremely halophilic archaeon from Algerian hypersaline environment.
  • authors: Barreteau H, Vandervennet M, Guedon L, Point V, Canaan S, Rebuffat S, Peduzzi J, Carre-Mlouka A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003211
  • year: 2019
  • mesh: Algeria, Base Composition, DNA, Archaeal/genetics, Fatty Acids/chemistry, Haloarcula/*classification/isolation & purification, Lakes/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, *Saline Waters, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67819Hélène Barreteau, Manon Vandervennet, Laura Guédon, Vanessa Point, Stéphane Canaan, Sylvie Rebuffat, Jean Peduzzi, Alyssa Carré-MloukaHaloarcula sebkhae sp. nov., an extremely halophilic archaeon from Algerian hypersaline environment10.1099/ijsem.0.003211IJSEM 69: 732-738 2019
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG