Strain identifier

BacDive ID: 16229

Type strain: Yes

Species: Streptomyces griseus

Strain Designation: C1, 18-16, D 100, C1

Strain history: CIP <- 1997, IFO <- 1994, ATCC <- R.E. Gordon: strain IMRU 3463

NCBI tax ID(s): 1911 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 9396

BacDive-ID: 16229

DSM-Number: 40236

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, thermophilic, Gram-positive, antibiotic compound production, human pathogen

description: Streptomyces griseus C1 is an obligate aerobe, spore-forming, thermophilic human pathogen that produces antibiotic compounds and was isolated from soil.

NCBI tax id

  • NCBI tax id: 1911
  • Matching level: species

strain history

@refhistory
9396<- E. B. Shirling, ISP <- S. A. Waksman, IMRU
67770KCC S-0047 <-- Y. Okami (NIHJ 106) <-- IMRU 3463.
119747CIP <- 1997, IFO <- 1994, ATCC <- R.E. Gordon: strain IMRU 3463

doi: 10.13145/bacdive16229.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces griseus
  • full scientific name: Streptomyces griseus (Krainsky 1914) Waksman and Henrici 1948 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Actinomyces globisporus subsp. flavofuscus
    20215Actinomyces acrimycini
    20215Actinomyces setonii
    20215Actinomyces fimicarius
    20215Streptomyces setonii
    20215Streptomyces fimicarius
    20215Streptomyces globisporus subsp. flavofuscus
    20215Streptomyces baarnensis
    20215Streptomyces acrimycini
    20215Actinomyces griseus
    20215Streptomyces erumpens
    20215Streptomyces argenteolus
    20215Streptomyces flavofuscus
    20215Streptomyces caviscabies

@ref: 9396

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces griseus subsp. griseus

full scientific name: Streptomyces griseus subsp. griseus (Krainsky 1914) Pridham 1970

strain designation: C1, 18-16, D 100, C1

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
119747positiverod-shapedno

colony morphology

  • @ref: 119747

multimedia

  • @ref: 9396
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40236.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37968MEDIUM 116 - for Streptomyces, Nocardia, Streptosporangium and Mycobacterium chlorophenolicumyesDistilled water make up to (1000.000 ml);Agar (20.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g)
9396GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yesName: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/65
9396ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yesName: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled waterhttps://mediadive.dsmz.de/medium/84
119747CIP Medium 194yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=194
119747CIP Medium 116yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=116

culture temp

@refgrowthtypetemperaturerange
22953nogrowth45thermophilic
37968positivegrowth30mesophilic
9396positivegrowth28mesophilic
67770positivegrowth28mesophilic
119747positivegrowth10-37
119747nogrowth41thermophilic
119747nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119747
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes100

compound production

@refcompound
9396restriction endonuclease SgrI
9396streptomycin
9396cycloheximide
20216Streptomycin
20216Cycloheximid
20216Endonuclease SgrI

halophily

@refsaltgrowthtested relationconcentration
22953NaClpositivegrowth7 %(w/v)
119747NaClpositivegrowth0-8 %
119747NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H8), MK-9(H6), MK-9(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2295330089acetate+growth
2295316708adenine+hydrolysis
2295318305arbutin+builds acid from
2295316947citrate+growth
2295315824D-fructose+growth
2295312936D-galactose+growth
2295317634D-glucose+growth
2295317716lactose+growth
2295316899D-mannitol+growth
2295316024D-mannose+growth
2295316634raffinose+growth
2295316988D-ribose+growth
2295365327D-xylose+growth
2295315740formate+growth
2295317368hypoxanthine+hydrolysis
2295337657methyl D-glucoside+growth
2295317272propionate+growth
2295353424tween 20+hydrolysis
2295353426tween 80+hydrolysis
2295315318xanthine+hydrolysis
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837117151xylitol-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
11974716947citrate-carbon source
1197474853esculin+hydrolysis
119747606565hippurate-hydrolysis
11974717632nitrate-reduction
11974716301nitrite-reduction
11974717632nitrate-respiration

antibiotic resistance

  • @ref: 119747
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6777017076streptomycinyes
11974735581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11974715688acetoin+
11974717234glucose-

enzymes

@refvalueactivityec
119747oxidase-
119747beta-galactosidase+3.2.1.23
119747alcohol dehydrogenase-1.1.1.1
119747gelatinase+
119747amylase+
119747DNase+
119747caseinase+3.4.21.50
119747catalase+1.11.1.6
119747tween esterase+
119747gamma-glutamyltransferase+2.3.2.2
119747lecithinase+
119747lipase+
119747lysine decarboxylase-4.1.1.18
119747ornithine decarboxylase-4.1.1.17
119747phenylalanine ammonia-lyase-4.3.1.24
119747tryptophan deaminase-
119747urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119747-+++-+---+-----+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119747---------------------+/---+/-----------+/-+/--+/-----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119747+++++----+++---++-++-+----+----+-+---+-----------++--++-+--++-----------+--+-+-++---+-+-++++-++-++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
9396soil
24939contaminated sediment of RiverLianjiang River at GuiyuChinaCHNAsia
119747Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
9396yesyes1Risk group (German classification)
1197471Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces griseus subsp. griseus gene for 16S rRNA, partial sequence, strain: NBRC 12875AB1842111458ena67263
20218Streptomyces griseus 16S ribosomal RNA gene, partial sequenceAF0567111537ena1911
20218Streptomyces griseus subsp. griseus strain DSM40236 16S-23S intergenic spacer region, partial sequenceAF363492300ena67263
20218Streptomyces griseus subsp. griseus ISP 5236 16S ribosomal RNA gene, partial sequenceAY0943711448ena67263
20218Streptomyces griseus strain DSM 40236 16S ribosomal RNA gene, partial sequenceGU383152779ena1911
24939Sphingobium sp. C1 16S ribosomal RNA gene, partial sequenceKR7122721452nuccore1673076
9396Streptomyces griseus 16S ribosomal RNA gene, partial sequenceAY2076041490ena1911
67770Streptomyces griseus subsp. griseus gene for 16S rRNA, partial sequence, strain: NBRC 15744AB1846991465ena67263
67770S.griseus 16S, 23S, and 5S rRNA gene sequenceM763886869ena67263
67770S.griseus 16S, 23S, and 5S rRNA gene sequenceX554356869ena67263
67770S.griseus genes for 16S rRNA, 23S rRNA & 5S rRNAX614786869ena

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces griseus strain DSM 402361911.11wgspatric1911
66792Streptomyces griseus strain NCTC130331911.17wgspatric1911
66792Streptomyces griseus NCTC 130332897015371draftimg1911
66792Streptomyces griseus DSM 402362634166340draftimg1911
66792Streptomyces griseus griseus DSM 40236651716785draftimg67263
67770Streptomyces griseus DSM 40236GCA_900105705contigncbi1911
67770Streptomyces griseus subsp. griseus NRRL B-2682GCA_001723125scaffoldncbi67263
67770Streptomyces griseus NCTC13033GCA_900460065contigncbi1911

GC content

@refGC-contentmethod
2295371-72
6777072.2genome sequence analysis
6777072high performance liquid chromatography (HPLC)
6777071thermal denaturation, midpoint method (Tm)
6777071fluorimetric

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno93.73no
gram-positiveyes90.598no
anaerobicno99.1no
aerobicyes94.23no
halophileno93.502no
spore-formingyes94.69no
glucose-utilyes88.344yes
flagellatedno97.871no
thermophileno99.355no
glucose-fermentno86.901no

External links

@ref: 9396

culture collection no.: DSM 40236, ATCC 23345, ATCC 23921, CBS 905.68, ETH 4289, IFO 12875, IMRU 3463, ISP 5236, KCC S-0047, KCC S-0644, NBRC 12875, NCTC 13033, RIA 1176, Waksman 18-16, JCM 4047, BCRC 13478, CBS 160.45, CCT 4836, CCUG 11104, CECT 3330, CFBP 4546, CGMCC 4.1419, CIP 105124, HAMBI 2315, IAM 12311, IFO 15744, JCM 4644, KACC 20084, KCTC 9080, KCTC 9135, LMG 19302, MTCC 4734, NBIMCC 2442, NBRC 15744, NCIMB 13023, NRRL B-2682, PCM 2331, VKM Ac-800, VTT E-072676, VTT E-991424

straininfo link

  • @ref: 85290
  • straininfo: 13853

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism4629704Chromogenesis mirabilis in Streptomyces griseus.Arai T, Mikami YAppl Microbiol10.1128/am.24.5.768-771.19721972Agar, Amino Acids/pharmacology, Catechol Oxidase/metabolism, Cell-Free System, Culture Media, Cysteine/pharmacology, Dihydroxyphenylalanine, Melanins/*biosynthesis, Oxidoreductases/metabolism, Peptones/pharmacology, Species Specificity, Spectrophotometry, Stereoisomerism, Streptomyces/enzymology/growth & development/*metabolism, Streptomyces griseus/enzymology/growth & development/metabolism, Tritium, Tyrosine/metabolismEnzymology
Metabolism7026523Self-resistance of a Streptomyces which produces istamycins.Yamamoto H, Hotta K, Okami Y, Umezawa HJ Antibiot (Tokyo)10.7164/antibiotics.34.8241981Aminoglycosides/biosynthesis/pharmacology, Anti-Bacterial Agents/biosynthesis/*pharmacology, Drug Resistance, Microbial, Escherichia coli/metabolism, Peptide Biosynthesis, *Peptides, Ribosomes/metabolism, Streptomyces/*drug effects/metabolismPathogenicity
Enzymology7691689Gene organization and primary structure of a ribosomal RNA gene cluster from Streptomyces griseus subsp. griseus.Kim E, Kim H, Hong SP, Kang KH, Kho YH, Park YHGene10.1016/0378-1119(93)90510-a1993Base Sequence, Cloning, Molecular, DNA, Bacterial, DNA, Ribosomal, Molecular Sequence Data, *Multigene Family, Nucleic Acid Conformation, RNA Processing, Post-Transcriptional, RNA, Bacterial/chemistry/*genetics/metabolism, RNA, Ribosomal/chemistry/*genetics/metabolism, Sequence Homology, Nucleic Acid, Streptomyces griseus/*genetics, Transcription, GeneticGenetics
Enzymology8800814Evidence of a role for NAD+-glycohydrolase and ADP-ribosyltransferase in growth and differentiation of Streptomyces griseus NRRL B-2682: inhibition by m-aminophenylboronic acid.Penyige A, Deak E, Kalmanczhelyi A, Barabas GMicrobiology (Reading)10.1099/13500872-142-8-19371996Bacterial Proteins/isolation & purification/metabolism, Boronic Acids/*pharmacology, Enzyme Inhibitors/*pharmacology, NAD/metabolism, NAD+ Nucleosidase/antagonists & inhibitors/isolation & purification/*metabolism, Poly(ADP-ribose) Polymerase Inhibitors, Poly(ADP-ribose) Polymerases/isolation & purification/*metabolism, Spores, Bacterial, Streptomyces griseus/drug effects/enzymology/*growth & developmentMetabolism
Enzymology9720038Membrane-bound and extracellular beta-lactamase production with developmental regulation in Streptomyces griseus NRRL B-2682.Deak E, SzaboA I, Kalmaczhelyi A, Gal Z, Barabas G, Penyige AMicrobiology (Reading)10.1099/00221287-144-8-21691998Acetylmuramyl-Alanyl-Isoglutamine/pharmacology, Amino Acid Sequence, Anti-Bacterial Agents/pharmacology, Extracellular Space/*enzymology/microbiology, Membrane Proteins/*metabolism, Molecular Sequence Data, Mutation, N-Acetylmuramoyl-L-alanine Amidase/metabolism, Penicillin G/pharmacology, Peptide Fragments/pharmacology, *Peptides, Peptidoglycan/pharmacology, Protease Inhibitors/pharmacology, Spores, Bacterial/drug effects/enzymology, Streptomyces griseus/drug effects/*enzymology/physiology, beta-Lactamases/*biosynthesis/isolation & purificationMetabolism
Phylogeny19060058Taxonomic study of a chromomycin-producing strain and reclassification of Streptomyces cavourensis subsp. washingtonensis as a later synonym of Streptomyces griseus.Wu X, Wen Y, Qian C, Li O, Fang H, Chen WInt J Syst Evol Microbiol10.1099/ijs.0.65628-02008Chromomycins/*metabolism, Classification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Species Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Streptomyces/chemistry/*classification/genetics/*physiology, Streptomyces griseus/chemistry/*classification/genetics/*physiologyGenetics
Metabolism22556031Characterization of new class III lantibiotics--erythreapeptin, avermipeptin and griseopeptin from Saccharopolyspora erythraea, Streptomyces avermitilis and Streptomyces griseus demonstrates stepwise N-terminal leader processing.Voller GH, Krawczyk JM, Pesic A, Krawczyk B, Nachtigall J, Sussmuth RDChembiochem10.1002/cbic.2012001182012Alanine/*analogs & derivatives/chemistry/genetics/metabolism, Amino Acid Sequence, Anti-Bacterial Agents/*chemistry/metabolism, Bacteriocins/*chemistry/genetics/metabolism, Genes, Bacterial, Mass Spectrometry, Molecular Sequence Data, Saccharopolyspora/*chemistry/genetics/metabolism, Streptomyces/*chemistry/genetics/metabolism, Streptomyces griseus/*chemistry/genetics/metabolism, Sulfides/*chemistry/metabolismGenetics
Phylogeny31174336Different Secondary Metabolite Profiles of Phylogenetically almost Identical Streptomyces griseus Strains Originating from Geographically Remote Locations.Sottorff I, Wiese J, Lipfert M, Preusske N, Sonnichsen FD, Imhoff JFMicroorganisms10.3390/microorganisms70601662019

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9396Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40236)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40236
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22953Zhiheng Liu, Yanlin Shi, Yamei Zhang, Zhihong Zhou, Zhitang Lu, Wei Li, Ying Huang, Carlos Rodríguez, Michael Goodfellow10.1099/ijs.0.63654-0Classification of Streptomyces griseus (Krainsky 1914) Waksman and Henrici 1948 and related species and the transfer of 'Microstreptospora cinerea' to the genus Streptomyces as Streptomyces yanii sp. nov.IJSEM 55: 1605-1610 200516014489
37968Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17109
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
85290Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13853.1StrainInfo: A central database for resolving microbial strain identifiers
119747Curators of the CIPCollection of Institut Pasteur (CIP 105124)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105124