Strain identifier

BacDive ID: 16220

Type strain: Yes

Species: Streptomyces morookaensis

Strain Designation: SF-337

Strain history: CIP <- 2003, JCM <- 1983, KCC <- IFO <- SAJ <- ISP, Streptoverticillium morookaense <- ATCC <- Meiji Seika Kaisha Ltd: strain SF-337

NCBI tax ID(s): 1970 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9602

BacDive-ID: 16220

DSM-Number: 40503

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, antibiotic compound production

description: Streptomyces morookaensis SF-337 is an obligate aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 1970
  • Matching level: species

strain history

@refhistory
9602<- E.B. Shirling, ISP (Streptoverticillium morookaense) <- ATCC <- Kusaba & Co., SF-337
67770KCC S-0673 <-- Meiji Seika Kaisha, Ltd.; SF-337.
121146CIP <- 2003, JCM <- 1983, KCC <- IFO <- SAJ <- ISP, Streptoverticillium morookaense <- ATCC <- Meiji Seika Kaisha Ltd: strain SF-337

doi: 10.13145/bacdive16220.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces morookaensis
  • full scientific name: Streptomyces morookaensis corrig. (Locci and Schofield 1989 ex Niida et al. 1963) Witt and Stackebrandt 1991
  • synonyms

    @refsynonym
    20215Streptoverticillium morookaense
    20215Streptomyces morookaense

@ref: 9602

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces morookaense

full scientific name: Streptomyces morookaense (Locci and Schofield 1989) Witt and Stackebrandt 1991

strain designation: SF-337

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
121146positivefilament-shapedno

colony morphology

@refcolony colorincubation periodmedium usedhemolysis ability
19813Brown10-14 daysISP 2
1981310-14 daysISP 3
1981310-14 daysISP 4
1981310-14 daysISP 5
19813Beige10-14 daysISP 6
19813Brown10-14 daysISP 7
1211461

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19813yesAerial MyceliumWhiteISP 2
19813yesAerial MyceliumISP 3
19813yesAerial MyceliumSparse greyISP 4
19813yesAerial MyceliumSparse whiteISP 5
19813noISP 6
19813yesAerial MyceliumSparse whiteISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9602GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19813ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19813ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19813ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19813ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19813ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19813ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33897MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
9602STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf
121146CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
9602positivegrowth28mesophilic
19813positiveoptimum28mesophilic
33897positivegrowth30mesophilic
67770positivegrowth28mesophilic
121146positivegrowth25-37mesophilic
121146nogrowth10psychrophilic
121146nogrowth41thermophilic
121146nogrowth45thermophilic

Physiology and metabolism

tolerance

  • @ref: 19813
  • compound: Lysozyme
  • percentage: 1

oxygen tolerance

  • @ref: 121146
  • oxygen tolerance: obligate aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
19813Formation of spore chains (verticillati)(Ve), spore surface smoothsporeyes
69481yes100
69480yes100

compound production

@refcompound
96028 azaguanine
9602blasticidin S
9602nucleotide pyrophosphokinase

halophily

@refsaltgrowthtested relationconcentration
19813NaClpositivemaximum5 %
121146NaClpositivegrowth0-4 %
121146NaClnogrowth6 %
121146NaClnogrowth8 %
121146NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1981362968cellulose+/-
1981316634raffinose+/-
1981326546rhamnose+/-
1981328757fructose+
1981329864mannitol+
1981317268myo-inositol+
1981318222xylose+/-
1981317992sucrose+/-
1981322599arabinose+/-
1981317234glucose+/-
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
12114616947citrate-carbon source
1211464853esculin+hydrolysis
121146606565hippurate-hydrolysis
12114617632nitrate+reduction
12114616301nitrite-reduction
12114617632nitrate-respiration

antibiotic resistance

  • @ref: 121146
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
67770634868-azaguanineyes
6777015353blasticidin Syes
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
12114635581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
12114615688acetoin-
12114617234glucose-

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
121146oxidase-
121146beta-galactosidase-3.2.1.23
121146alcohol dehydrogenase-1.1.1.1
121146gelatinase-
121146amylase+
121146DNase-
121146caseinase-3.4.21.50
121146catalase+1.11.1.6
121146tween esterase-
121146gamma-glutamyltransferase+2.3.2.2
121146lecithinase+
121146lipase+
121146lysine decarboxylase-4.1.1.18
121146ornithine decarboxylase-4.1.1.17
121146phenylalanine ammonia-lyase-4.3.1.24
121146protease+
121146tryptophan deaminase-
121146urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121146-+++++++++++---++++-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19813------+--+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121146+++++----++--------+-----------+++-------------------------++-----------++-+---++---+-+--+++-++-+++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
9602soil
67770Soil, orange orchardOomura, Nagasaki Pref.JapanJPNAsia
121146Environment, Soil at orange orchardOomura, NagasakiJapanJPNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
96021Risk group (German classification)
198131Risk group (German classification)
1211461Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces morookaense strain DSM 40503 16S ribosomal RNA gene, partial sequenceAY9998421420ena1970
20218Streptomyces morookaense gene for 16S ribosomal RNA, partial sequence, strain: JCM 4673D44300120ena1970
20218Streptomyces morookaense 16S rRNA gene, type strain LMG 20074TAJ7813491478ena1970
20218Streptomyces morookaense gene for 16S rRNA, partial sequence, strain: NBRC 13416AB1848781477ena1970

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces morookaense JCM 4793GCA_014656115scaffoldncbi1970
66792Streptomyces morookaense DSM 40503GCA_013030945contigncbi1970
66792Streptomyces morookaense strain DSM 405031970.3wgspatric1970
66792Streptomyces morookaense strain JCM 47931970.4wgspatric1970

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedno98.295no
gram-positiveyes87.89no
anaerobicno99.137no
aerobicyes89.12no
halophileno94.034no
spore-formingyes92.671no
glucose-utilyes90.87no
thermophileno95.877no
motileno92.648no
glucose-fermentno89.079no

External links

@ref: 9602

culture collection no.: DSM 40503, ATCC 19166, CBS 717.72, IFO 13416, ISP 5503, NBRC 13416, RIA 1377, JCM 4673, BCRC 15169, CGMCC 4.1986, CIP 108145, JCM 4793, KCTC 19944, NRRL B-12429, VKM Ac-1916, KCC S-0793

straininfo link

  • @ref: 85281
  • straininfo: 45613

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology9538199Purification and characterization of a puromycin-hydrolyzing enzyme from blasticidin S-producing Streptomyces morookaensis.Nishimura M, Matsuo H, Nakamura A, Sugiyama MJ Biochem10.1093/oxfordjournals.jbchem.a0219291998Aminopeptidases/antagonists & inhibitors/chemistry/isolation & purification, Enzyme Inhibitors/pharmacology, Hydrolysis, Metals, Heavy/pharmacology, Molecular Sequence Data, Nucleosides/biosynthesis, Puromycin/*metabolism, Streptomyces/*enzymology, Substrate SpecificityGenetics
Enzymology16503293Molecular cloning and characterization of gene encoding novel puromycin-inactivating enzyme from blasticidin S-producing Streptomyces morookaensis.Nishimura M, Ikeda K, Sugiyama MJ Biosci Bioeng10.1263/jbb.101.632006Amino Acid Sequence, Aminopeptidases/biosynthesis/*chemistry/*genetics, Cloning, Molecular, Escherichia coli/genetics/metabolism, *Genes, Bacterial, Molecular Sequence Data, Nucleosides/biosynthesis, Puromycin/*metabolism, Streptomyces/*enzymology/genetics, Substrate SpecificityMetabolism
Phylogeny20173013Streptomyces lacticiproducens sp. nov., a lactic acid-producing streptomycete isolated from the rhizosphere of tomato plants.Zhu HH, Yao Q, Yang SZ, Li ZK, Guo JInt J Syst Evol Microbiol10.1099/ijs.0.019125-02010Anti-Infective Agents/metabolism, China, Cluster Analysis, Culture Media/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Lycopersicon esculentum/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Spores, Bacterial/cytology, Streptomyces/*classification/genetics/*isolation & purification/physiologyGenetics
Genetics32732231Draft Genome Sequence of Streptomyces morookaense DSM 40503, an 8-Azaguanine-Producing Strain.Hei Y, Li Z, Zhou Y, Hu C, Gao JM, Qi JMicrobiol Resour Announc10.1128/MRA.00518-202020

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9602Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40503)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40503
19813Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40503.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33897Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5735
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
85281Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45613.1StrainInfo: A central database for resolving microbial strain identifiers
121146Curators of the CIPCollection of Institut Pasteur (CIP 108145)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108145