Strain identifier
BacDive ID: 162
Type strain:
Species: Schaalia georgiae
Strain Designation: D145A-07
Strain history: CIP <- 1996, ATCC <- L.V. Moore: strain VPI D145A-7 <- W.E.C. Moore
NCBI tax ID(s): 1120942 (strain), 52768 (species)
General
@ref: 2851
BacDive-ID: 162
DSM-Number: 6843
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, colony-forming
description: Schaalia georgiae D145A-07 is an anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from human, healthy adult, gingival crevice.
NCBI tax id
NCBI tax id | Matching level |
---|---|
52768 | species |
1120942 | strain |
strain history
@ref | history |
---|---|
2851 | <- ATCC <- VPI, D145A-07 |
119602 | CIP <- 1996, ATCC <- L.V. Moore: strain VPI D145A-7 <- W.E.C. Moore |
doi: 10.13145/bacdive162.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Actinomycetales
- family: Actinomycetaceae
- genus: Schaalia
- species: Schaalia georgiae
- full scientific name: Schaalia georgiae (Johnson et al. 1990) Nouioui et al. 2018
synonyms
- @ref: 20215
- synonym: Actinomyces georgiae
@ref: 2851
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Actinomycetaceae
genus: Schaalia
species: Schaalia georgiae
full scientific name: Schaalia georgiae (Johnson et al. 1990) Nouioui et al. 2018
strain designation: D145A-07
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
23355 | positive | 3.3-6.1 µm | 0.9-1.0 µm | rod-shaped | no | |
69480 | no | 93.732 | ||||
69480 | positive | 100 | ||||
119602 | positive | rod-shaped | no |
colony morphology
@ref | colony size | colony color | colony shape | medium used | hemolysis ability |
---|---|---|---|---|---|
23355 | 1.0 mm | white | circular | peptone-yeast extract-glucose broth | |
23355 | 1 mm | opaque | circular | ||
119602 | 1 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
2851 | BHI/2 MEDIUM (DSMZ Medium 217) | yes | https://mediadive.dsmz.de/medium/217 | Name: BHI/2 MEDIUM (DSMZ Medium 217) Composition: Brain heart infusion 37.0 g/l Casein hydrolysate 10.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water |
23355 | peptone-yeast extract-glucose broth | yes | ||
23355 | peptone-yeast extract medium | yes | ||
39089 | MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria | yes | Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml) | |
119602 | CIP Medium 10 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
2851 | positive | growth | 37 | mesophilic |
23355 | growth | 37 | mesophilic | |
23355 | positive | growth | 37 | mesophilic |
39089 | positive | growth | 37 | mesophilic |
119602 | positive | growth | 37-41 | |
119602 | no | growth | 10 | psychrophilic |
119602 | no | growth | 25 | mesophilic |
119602 | no | growth | 30 | mesophilic |
culture pH
- @ref: 23355
- ability: positive
- type: growth
- pH: 4.5-7.8
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
2851 | anaerobe | |
23355 | facultative anaerobe | |
69480 | anaerobe | 94.568 |
119602 | facultative anaerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
23355 | no | |
69481 | no | 97 |
69480 | no | 99.998 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
119602 | NaCl | positive | growth | 0-2 % |
119602 | NaCl | no | growth | 4 % |
119602 | NaCl | no | growth | 6 % |
119602 | NaCl | no | growth | 8 % |
119602 | NaCl | no | growth | 10 % |
murein
- @ref: 2851
- murein short key: A21.11
- type: A5ß L-Orn-L-Lys-D-Glu
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23355 | 27613 | amygdalin | - | builds acid from |
23355 | 22599 | arabinose | - | builds acid from |
23355 | 17057 | cellobiose | - | builds acid from |
23355 | 4853 | esculin | - | builds acid from |
23355 | 17268 | myo-inositol | - | builds acid from |
23355 | 29864 | mannitol | - | builds acid from |
23355 | 37684 | mannose | - | builds acid from |
23355 | 6731 | melezitose | - | builds acid from |
23355 | 28053 | melibiose | - | builds acid from |
23355 | 16634 | raffinose | - | builds acid from |
23355 | 17814 | salicin | - | builds acid from |
23355 | 30911 | sorbitol | - | builds acid from |
23355 | 17992 | sucrose | - | builds acid from |
23355 | 28017 | starch | - | hydrolysis |
23355 | 17632 | nitrate | - | reduction |
23355 | 30089 | acetate | + | builds acid from |
23355 | 15740 | formate | + | builds acid from |
23355 | 24996 | lactate | + | builds acid from |
23355 | 29864 | mannitol | + | builds acid from |
23355 | 15361 | pyruvate | + | builds acid from |
23355 | 16634 | raffinose | + | builds acid from |
23355 | 30031 | succinate | + | builds acid from |
23355 | 28757 | fructose | + | builds acid from |
23355 | 17234 | glucose | + | builds acid from |
23355 | 28087 | glycogen | + | builds acid from |
23355 | 17716 | lactose | + | builds acid from |
23355 | 17306 | maltose | + | builds acid from |
23355 | 26546 | rhamnose | + | builds acid from |
23355 | 33942 | ribose | + | builds acid from |
23355 | 28017 | starch | + | builds acid from |
23355 | 27082 | trehalose | + | builds acid from |
23355 | 18222 | xylose | + | builds acid from |
23355 | 53426 | tween 80 | + | growth |
23355 | 17754 | glycerol | +/- | fermentation |
23355 | 4853 | esculin | +/- | hydrolysis |
23355 | 5291 | gelatin | +/- | hydrolysis |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | 24265 | gluconate | - | builds acid from |
68371 | 18403 | L-arabitol | - | builds acid from |
68371 | 18333 | D-arabitol | - | builds acid from |
68371 | 18287 | L-fucose | - | builds acid from |
68371 | 28847 | D-fucose | - | builds acid from |
68371 | 16443 | D-tagatose | - | builds acid from |
68371 | 62318 | D-lyxose | - | builds acid from |
68371 | 28066 | gentiobiose | - | builds acid from |
68371 | 17151 | xylitol | - | builds acid from |
68371 | 16634 | raffinose | - | builds acid from |
68371 | 6731 | melezitose | - | builds acid from |
68371 | 15443 | inulin | - | builds acid from |
68371 | 28053 | melibiose | - | builds acid from |
68371 | 17716 | lactose | - | builds acid from |
68371 | 17057 | cellobiose | - | builds acid from |
68371 | 17814 | salicin | - | builds acid from |
68371 | 4853 | esculin | - | builds acid from |
68371 | 18305 | arbutin | - | builds acid from |
68371 | 27613 | amygdalin | - | builds acid from |
68371 | 59640 | N-acetylglucosamine | - | builds acid from |
68371 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
68371 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
68371 | 17924 | D-sorbitol | - | builds acid from |
68371 | 16899 | D-mannitol | - | builds acid from |
68371 | 17268 | myo-inositol | - | builds acid from |
68371 | 16813 | galactitol | - | builds acid from |
68371 | 62345 | L-rhamnose | - | builds acid from |
68371 | 17266 | L-sorbose | - | builds acid from |
68371 | 16024 | D-mannose | - | builds acid from |
68371 | 12936 | D-galactose | - | builds acid from |
68371 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
68371 | 15963 | ribitol | - | builds acid from |
68371 | 65328 | L-xylose | - | builds acid from |
68371 | 65327 | D-xylose | - | builds acid from |
68371 | 16988 | D-ribose | - | builds acid from |
68371 | 30849 | L-arabinose | - | builds acid from |
68371 | 17108 | D-arabinose | - | builds acid from |
68371 | 17113 | erythritol | - | builds acid from |
68371 | 17754 | glycerol | - | builds acid from |
119602 | 16947 | citrate | - | carbon source |
119602 | 4853 | esculin | - | hydrolysis |
119602 | 606565 | hippurate | - | hydrolysis |
119602 | 17632 | nitrate | - | reduction |
119602 | 16301 | nitrite | - | reduction |
119602 | 17632 | nitrate | + | respiration |
68380 | 16199 | urea | - | hydrolysis |
68380 | 29016 | arginine | - | hydrolysis |
68380 | 16024 | D-mannose | - | fermentation |
68380 | 29985 | L-glutamate | - | degradation |
68380 | 17632 | nitrate | - | reduction |
68380 | 27897 | tryptophan | - | energy source |
antibiotic resistance
- @ref: 119602
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
23355 | 30031 | succinate | yes |
23355 | 24996 | lactate | yes |
23355 | 15740 | formate | yes |
23355 | 30089 | acetate | yes |
119602 | 35581 | indole | no |
68380 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | indole test | voges-proskauer-test | methylred-test |
---|---|---|---|---|---|
68380 | 35581 | indole | - | ||
119602 | 15688 | acetoin | - | ||
119602 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
23355 | catalase | - | 1.11.1.6 |
23355 | urease | - | 3.5.1.5 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68380 | serine arylamidase | + | |
68380 | glutamyl-glutamate arylamidase | - | |
68380 | histidine arylamidase | + | |
68380 | alanine arylamidase | + | 3.4.11.2 |
68380 | tyrosine arylamidase | + | |
68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68380 | leucine arylamidase | + | 3.4.11.1 |
68380 | phenylalanine arylamidase | + | |
68380 | proline-arylamidase | + | 3.4.11.5 |
68380 | L-arginine arylamidase | + | |
68380 | alkaline phosphatase | - | 3.1.3.1 |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | alpha-fucosidase | - | 3.2.1.51 |
68380 | glutamate decarboxylase | - | 4.1.1.15 |
68380 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68380 | beta-glucuronidase | - | 3.2.1.31 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | beta-glucosidase | - | 3.2.1.21 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | alpha-galactosidase | - | 3.2.1.22 |
68380 | arginine dihydrolase | - | 3.5.3.6 |
68380 | urease | - | 3.5.1.5 |
119602 | oxidase | - | |
119602 | beta-galactosidase | + | 3.2.1.23 |
119602 | alcohol dehydrogenase | - | 1.1.1.1 |
119602 | gelatinase | - | |
119602 | amylase | + | |
119602 | DNase | - | |
119602 | caseinase | - | 3.4.21.50 |
119602 | catalase | - | 1.11.1.6 |
119602 | tween esterase | - | |
119602 | gamma-glutamyltransferase | - | 2.3.2.2 |
119602 | lecithinase | - | |
119602 | lipase | - | |
119602 | lysine decarboxylase | - | 4.1.1.18 |
119602 | ornithine decarboxylase | - | 4.1.1.17 |
119602 | phenylalanine ammonia-lyase | - | 4.3.1.24 |
119602 | tryptophan deaminase | - | |
119602 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119602 | - | - | + | + | - | + | - | - | - | - | - | - | - | + | - | + | - | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119602 | - | - | - | - | - | - | - | - | - | - | +/- | +/- | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | +/- | - | - | +/- | +/- | - | - | - | +/- | +/- | - | - | +/- | - | - | - | - | - | - | - | - | +/- |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2851 | - | - | - | +/- | - | +/- | - | - | - | - | - | +/- | - | - | - | - | - | + | + | +/- | + | + | - | + | + | +/- | + | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent |
---|---|---|---|---|
2851 | human, healthy adult, gingival crevice | |||
51268 | Human,healthy adult,gingival crevice | |||
119602 | Human, Healthy subgingival plaque | United States of America | USA | North America |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Human | |
#Host Body-Site | #Oral cavity and airways | #Gingiva |
taxonmaps
- @ref: 69479
- File name: preview.99_6373.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_224;96_1617;97_1934;98_2378;99_6373&stattab=map
- Last taxonomy: Schaalia georgiae
- 16S sequence: X80413
- Sequence Identity:
- Total samples: 14712
- soil counts: 158
- aquatic counts: 197
- animal counts: 14246
- plant counts: 111
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
2851 | 1 | Risk group (German classification) |
119602 | 2 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 20218
- description: A. georgiae 16S rDNA, type strain DSM 6843T
- accession: X80413
- length: 1422
- database: ena
- NCBI tax ID: 52768
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Schaalia georgiae DSM 6843 | GCA_000429245 | scaffold | ncbi | 1120942 |
66792 | Actinomyces georgiae DSM 6843 | 1120942.4 | wgs | patric | 1120942 |
66792 | Actinomyces georgiae DSM 6843 | 2523231019 | draft | img | 1120942 |
GC content
@ref | GC-content | method |
---|---|---|
2851 | 65.0 | thermal denaturation, midpoint method (Tm) |
23355 | 68.00 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 97 | no |
motile | no | 93.922 | yes |
flagellated | no | 97.904 | no |
gram-positive | yes | 94.217 | yes |
anaerobic | yes | 66.586 | no |
aerobic | no | 96.193 | no |
halophile | no | 92.421 | no |
spore-forming | no | 92.581 | yes |
thermophile | no | 79.086 | yes |
glucose-util | yes | 90.104 | no |
glucose-ferment | yes | 83.835 | no |
External links
@ref: 2851
culture collection no.: DSM 6843, ATCC 49285, CCUG 32935, VPI D145A-07, CIP 104749, VPI D145A-7
straininfo link
- @ref: 69844
- straininfo: 42400
literature
- topic: Phylogeny
- Pubmed-ID: 2397195
- title: Actinomyces georgiae sp. nov., Actinomyces gerencseriae sp. nov., designation of two genospecies of Actinomyces naeslundii, and inclusion of A. naeslundii serotypes II and III and Actinomyces viscosus serotype II in A. naeslundii genospecies 2.
- authors: Johnson JL, Moore LV, Kaneko B, Moore WE
- journal: Int J Syst Bacteriol
- DOI: 10.1099/00207713-40-3-273
- year: 1990
- mesh: Actinomyces/*classification/genetics, DNA, Bacterial/isolation & purification/metabolism, Nucleic Acid Hybridization, Serotyping
- topic2: Enzymology
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
2851 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6843) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-6843 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
23355 | J. L. Johnson, Lillian V. H. Moore, Beverly Kaneko, W. E. C. Moore | 10.1099/00207713-40-3-273 | Actinomyces georgiae sp. nov., Actinomyces gerencseriae sp. nov., Designation of Two Genospecies of Actinomyces naeslundii, and Inclusion of A. naeslundii serotypes II and III and Actinomyces viscosus serotype II in A. naeslundii Genospecies 2 | IJSEM 40: 273-286 1990 | 2397195 | |
39089 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/16692 | ||||
51268 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 32935) | https://www.ccug.se/strain?id=32935 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68371 | Automatically annotated from API 50CH acid | |||||
68380 | Automatically annotated from API rID32A | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
69844 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42400.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
119602 | Curators of the CIP | Collection of Institut Pasteur (CIP 104749) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104749 |